• Proceedings of the Botanical Society of Korea Conference
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• 1999.04a
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• pp.18-18
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• 1999

We characterized a cDNA clone for a low molecular weight heat-shock protein (LMW HSP) from tobacco named TLHS-l. Nucleotide sequence determination of TLHS-1 identified an open reading frame for 159 amino acids. To the upstream of the open reading frame, a sequence of 124 nucleotides was determined. To the 3' downstream of the open reading frame, 212 nucleotides were identified which carried poly(A)-tail. Comparison of the open reading frame and hydropathy plot of TLHS-1 with the previously reported class I LMW HSPs showed high identity which classified TLHS-1 as a class I LMW HSP cDNA clone. We proposed that there are six consensus regions in class I LMW HSPs. RNA blot hybridization for TLHS-1 showed a typical expression pattern of heat-shock-inducible gene from three common tobacco cultivars. The open reading frame of TLHS-1 was overexpressed in Escherichia coli. TLHS-1 protein confers thermal protection of other proteins in vitro and in vivo. Thermal induced aggregation of citrate synthase was reduced by purified TLHS-1 protein, and thermal death rate at $50^{\circ}C$ was reduced in E. coli expressing TLHS-l. From these data, we can expect that TLHS-1 acts as a molecular chaperone.perone.

• Nuclear Engineering and Technology
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• v.56 no.1
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• pp.141-146
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• 2024

Station blackout (SBO) risk is one of the most significant contributors to nuclear power plant risk. In this paper, the sequence probability formulas derived by the convolution approach are compared with those derived by the conventional event tree/fault tree (ET/FT) approach for the SBO situation in which emergency diesel generators fail to start. The comparison identifies what makes the ET/FT approach more conservative and raises the issue regarding the mission time of a turbine-driven auxiliary feedwater pump (TDP), which suggests a possible modeling improvement in the ET/FT approach. Monte Carlo simulations with up-to-date component reliability data validate the convolution approach. The sequence probability of an alternative alternating current diesel generator (AAC DG) failing to start and the TDP failing to operate owing to battery depletion contributes most to the SBO risk. The probability overestimation of the scenario in which the AAC DG fails to run and the TDP fails to operate owing to battery depletion contributes most to the SBO risk overestimation determined by the ET/FT approach. The modification of the TDP mission time renders the sequence probabilities determined by the ET/FT approach more consistent with those determined by the convolution approach.

• Microbiology and Biotechnology Letters
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• v.22 no.2
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• pp.134-142
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• 1994

The neucleotide sequences of the xylA gene encoding $\beta $-xylosidase of Bacillus stearothermophilus and is its flanking regions were datermined. Three open reading frame(ORFs) were found, one of which(ORF1) appeared to code for the $\beta $-xylosidase. The 1830 base pair ORF1 encoded 609 amino acids starting from a TTG initiation codon. The molecular weight deduced from the nucleotide sequence(68 KD) was in agreement with that estimated by SDS-polyacrylamide gel electrophoresis of the purified enzyme(66 KD). The Shine-Dalgarno sequence(5'-AGGAGG-3') was found 11 bp upstream of the initiation codon. Further 15 bp upstream, there observed a potential transcription initiation signals. The putative -10 sequence(CATAAT) and -35 sequence(TTGTTA) coresponded closely to the consensus sequences for Bacillus subtilis RNA polymerase with major sigma factor. The guanine-plus-cytosine content of the coding region of the xylA gene was 56mol% while that of the third position of the codons was 63 mol%. Based on the comparison with the amino acid sequences of several other carbohydrate degrading enzymes, two conserved regions, possibly participating in the catalytic mechamism of $\beta $-xylosidase xylA, were identified in 278-298 and 329-350 regions of the translated xylA gene. The nucleotide sequence of the xylA was found to exhibit no homology to any other genes so far reproted.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.27 no.2
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• pp.62-68
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• 2013

The dynamic voltage restorer (DVR) is an effective protection device for wind turbine generators based on doubly-fed induction generator (DFIG) that is operated under unbalanced voltage dip conditions. The compensating voltages of the DVR depend on the voltage dips and on the influence of the zero sequence component. The zero sequence component results in high insulation costs and asymmetry in terminal voltages. This paper proposes the use of a proportional-resonant controller in stationary reference frames for controlling zero sequence components in the DVR to protect the DFIG during unbalanced voltage dips. To enhance the proposed control method, a comparison is carried out between two cases: with and without using the control of a zero sequence component. Simulation results are presented to verify the effectiveness of the proposed control method by using the Psim simulation program.

• Fisheries and Aquatic Sciences
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• v.5 no.1
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• pp.54-58
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• 2002

The polymerase chain reaction (PCR) amplification technique was used for comparison of Listeria monocytogenes serotypes. PCR primers for the fragment of invasion-associated protein (iap) gene were highly specific for all the serotypes of L. monocytogenes. Other Listeria spp., such as Listeria ivanovii and Listeria innocua were not produced the PCR fragments by above primer set. The nucleotide sequences of PCR products showed high homologies in comparison of all the isolated serotypes except unknown type II-2. The deduced amino acid sequences of the PCR products also showed similar to one another. The various region of the PCR products, called a Thr-Asn repeat region was presented. All of isolated L. monocytogenes serotypes possessed 16 to 20 Thr-Asn repeats.

• Journal of Welding and Joining
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• v.16 no.6
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• pp.104-112
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• 1998

In the previous study, the countermeasure for welding deformation of bracket tilt is through up through experimental inspection for total process including welding process. For completeness of systematic examination of parts having sensitivity on welding deformation, the comparison and feedback between the result through simulation of welding process and experimental data is needed. In other words, it is necessary to control welding deformation that construct the prediction system for welding deformation through comparison and tuning with experimental data. In the present study, the application of FEA on welding process of bracket tilt with susceptibility to deformation is made and deformation behavior through change of welding sequence is focused on. It is used to improve the exactness of deformation analysis that three dimensional analysis for moving heat source, activated and deactivated bead element, and volume heat flux etc.

• Bulletin of the Korean Mathematical Society
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• v.51 no.4
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• pp.957-964
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• 2014

In this paper, we prove that there is no branch point in the Lorentz space (M, d) which is the limit space of a sequence {($M_{\alpha},d_{\alpha}$)} of compact globally hyperbolic interpolating spacetimes with $C^{\pm}_{\alpha}$-properties and curvature bounded below. Using this, we also obtain that every maximal timelike geodesic in the limit space (M, d) can be expressed as the limit curve of a sequence of maximal timelike geodesics in {($M_{\alpha},d_{\alpha}$)}. Finally, we show that the limit space (M, d) satisfies a timelike triangle comparison property which is analogous to the case of Alexandrov curvature bounds in length spaces.

• Plant Resources
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• v.1 no.2
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• pp.92-97
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• 1998

An isolate of barley yellow mosaic virus(BaYMV-HN) obtained from Haenam, Korea was compared with two BaYMV strains. BaYMV-Ⅱ-1 from Japan and BaYMV-G from Germany. The sequence of the 3'-terminal 3817nucleotides[excluding the poly (A) tail] of RNA 1 of BaYMV-HN was determined to start within a long open reading frame coding for a part of the NIa-VPg polymerase(26 amino acids). NIa-Pro polymerase (343 amino acids), NIb polymerase(528 amino acids) and the entire capsid protein(297 amino acids), which is followed by a noncoding region(NCR) of 235 nucelotides. In the partial ORFs, BaYMV-HN shows higher sequence homology with BaYMV-Ⅱ-1(99.5%) than BaYMV-G(92.7%). The 3' non-coding regions of BaYMV-HN(235nt) shows higher nucleotide sequence homology with BaYMV-G(235nt)(99.6%) than BaYMV-Ⅱ-1(231nt)(97.0%). The 3' NIa-Pro protein sequence of BaYMV-HN shows higher amino acid sequence homology with BaYMV-Ⅱ-1(95.0%) than BaYMV-G(93.6%), but, NIb protein sequence of BaYMV-HN shows same all amino acid sequence. The capsid protein sequence of BaYMV-HN(297aa) shows same with BaYMV-Ⅱ-1, and shows higher nucleotide sequence homology with BaYMV-UK (from United Kingdom)(97.3%) than BaYMV-G(96.9%) and G2(96.9%). Difference of capsid protein amino acid were 0-9 between the Japan, United Kingdom and Germany and were 2-6 between all Korean isolates. Many of the amino acid differences are located in the N-terminal regions of the capsid proteins from 1 to 74 amino acid positions.

• The KIPS Transactions:PartC
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• v.10C no.7
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• pp.899-914
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• 2003

It is generally accepted that self-similar (or fractal) processes may provide better models for teletraffic in modern telecommunication networks than Poisson Processes. If this is not taken into account, it can lead to inaccurate conclusions about performance of telecommunication networks. Thus, an important requirement for conducting simulation studies of telecommunication networks is the ability to generate long synthetic stochastic self-similar sequences. Three generators of pseudo-random self-similar sequences, based on the FFT〔20〕, RMD〔12〕 and SRA methods〔5, 10〕, are compared and analysed in this paper. Properties of these generators were experimentally studied in the sense of their statistical accuracy and times required to produce sequences of a given (long) length. While all three generators show similar levels of accuracy of the output data (in the sense of relative accuracy of the Horst parameter), the RMD- and SRA-based generators appear to be much faster than the generator based on FFT. Our results also show that a robust method for comparative studies of self-similarity in pseudo-random sequences is needed.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.90-90
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• 2002

In this study, we report the cloning of the porcine UPII genomic DNA, which contains a putative full-length open reading frame encoding the UPII protein. A comparison of the porcine UPII gene coding sequence with the previously published mouse UPII sequence demonstrates that only the exon sequences are partially conserved. Northern and immunohistochemical analyses show that the porcine UPII gene is expressed only in the urothelium and that the protein specifically localizes to urothelial superficial cells. (omitted)