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http://dx.doi.org/10.5657/fas.2002.5.1.054

Sequence Analysis of iap Gene PCR Products using Listeria monocytogenes Serotypes  

Kang Sun-Mo (Department of Microbiology, Pukyong National University)
Kang Ji-Hee (Department of Microbiology, Pukyong National University)
Lee Myung-Suk (Department of Microbiology, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.5, no.1, 2002 , pp. 54-58 More about this Journal
Abstract
The polymerase chain reaction (PCR) amplification technique was used for comparison of Listeria monocytogenes serotypes. PCR primers for the fragment of invasion-associated protein (iap) gene were highly specific for all the serotypes of L. monocytogenes. Other Listeria spp., such as Listeria ivanovii and Listeria innocua were not produced the PCR fragments by above primer set. The nucleotide sequences of PCR products showed high homologies in comparison of all the isolated serotypes except unknown type II-2. The deduced amino acid sequences of the PCR products also showed similar to one another. The various region of the PCR products, called a Thr-Asn repeat region was presented. All of isolated L. monocytogenes serotypes possessed 16 to 20 Thr-Asn repeats.
Keywords
Listeria monocytogenes; iap Gene; Serotype; PCR; Sequence analysis;
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