Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Sequence Analysis of iap Gene PCR Products using Listeria monocytogenes Serotypes

  • Kang Sun-Mo (Department of Microbiology, Pukyong National University) ;
  • Kang Ji-Hee (Department of Microbiology, Pukyong National University) ;
  • Lee Myung-Suk (Department of Microbiology, Pukyong National University)
  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The polymerase chain reaction (PCR) amplification technique was used for comparison of Listeria monocytogenes serotypes. PCR primers for the fragment of invasion-associated protein (iap) gene were highly specific for all the serotypes of L. monocytogenes. Other Listeria spp., such as Listeria ivanovii and Listeria innocua were not produced the PCR fragments by above primer set. The nucleotide sequences of PCR products showed high homologies in comparison of all the isolated serotypes except unknown type II-2. The deduced amino acid sequences of the PCR products also showed similar to one another. The various region of the PCR products, called a Thr-Asn repeat region was presented. All of isolated L. monocytogenes serotypes possessed 16 to 20 Thr-Asn repeats.

Keywords

References

  1. Allerberger, F., M. Dierich, G. Petranyi, M. Lalic and A. Bubert. 1997. Nongemolytic strains of Listeria monocyto-genes detected in milk products using V1DAS immuno-assay kit. Zentbl. Hyg., 200, 189-195
  2. Bubert, A., J. Riebe, N. Schnitzler, A. $Sch\ddot{o}nberg,$ W. Goebel and P. Schubert. 1997. Isolation of catalase-negative Listeria monocytogenes strains from listeriosis patients and their rapid identification by anti-p60 antibodies and/or PCR. J. Clin. Microbiol., 35, 179-183
  3. Bubert, A., S. Kohler and W. Goebel. 1992. The homologous and heterologous regions within the iap gene allow genes- and species- specific identification of Listeria spp. by polymerase chain reaction. Appl. Environ. Microbiol., 58, 2625-2632
  4. Datta, A.R. 1994. Factor controlling expression of virulence genes in Listeria monocytoeenes. Food Microbiol., 11, 123-129 https://doi.org/10.1006/fmic.1994.1016
  5. Elsner, H.A., I. Soboaka, A. Bubert- H. Albrecht, R. Laufs and D. Mack. 1996. Catalase-negative Listeria monocyto-genes causing lethal sepsis and meningitis in an adult heatologic patient. Eur. J. Clin. Microbiol. Infect. Dis., 15, 965-967 https://doi.org/10.1007/BF01690521
  6. Farber, J.M. and P.I. Peterkin. 1991. Listena monocytogenes, a food-borne pathogen. Microbiol. Review, 55, 476-511
  7. Goebel, W., S. Kathanou, M. Kuhn, Z. Sokolovic, J. Kreft, S. Kohler, D. Funke, T. Chakraborty and M. Leimeister-Wachter. 1988. Hemolysin from Listena-biochemistry, genetics and function in pathogenesis. Infection, 16(Suppl.2), 149-156 https://doi.org/10.1007/BF01639739
  8. Gutekunst, K.A., B.P. Holloway and G.M. Carlone. 1992. DNA sequence heterogeneity in the gene encoding a 60 kilodalton extracellular protein of Listeria monocyto-genes and other Listeria species. Can. J. MicrobioL, 38, 865-870 https://doi.org/10.1139/m92-141
  9. $K\ddot{o}hler,$ S., M. Leimeister-Wachter, T. Chakraborty, F. Lott-speich and W. Gobel. 1990. The gene coding for protein p60 of Listeria monocytosenes and its use as a specific probe for Listeria monocytogenes. Infect. Immun., 58, 1943-1950
  10. Kuhn, M. and W. Goebel. 1989. Identification of an extra-cellular protein of Listeria monocytosenes possibly involved in intracellular uptake by mammalian cell. Infect. Immun., 57, 55-61
  11. Manzano, M., L. Cocolin, C. Cantoni and G. Comi. 1998. Arapid method for the identification and partial serotyping of Listeiia moaocytogenes in food by PCR and restric-tion enzyme analysis, Int. J. Food Microbiol., 42, 207 -212 https://doi.org/10.1016/S0168-1605(98)00086-5
  12. $N\phirrung,$ B., J.K. Andersen and J. Schlundt. 1999. Incidence and control of Listeria monocytogenes in foods in Den-mark. Inter. J. Food Microbiol., 53, 195-203 https://doi.org/10.1016/S0168-1605(99)00157-9
  13. Rasmussen, O.F., P. Skouboe, L. Dons, L. Rossen and J.E. Olsen. 1995. Listeiia monocytogenes exists in at least three evolutionary lines: Evidence from flagellin, invasiva associated protein and listeriolysin O genes. Microbiol., 141, 2053-2061 https://doi.org/10.1099/13500872-141-9-2053