• Journal of Applied Biological Chemistry
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• 제43권4호
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• pp.240-245
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• 2000

Two genes encoding maltooligosyltrehalose synthase (SaMTS) and maltooligosyltrehalose trehalohydrolase (SaMTH) were isolated from a hyperthermophilic microorganism, Sulfolobus acidocaldarius (ATCC 49462). ORFs of the SaMTS and SaMTH genes are 2,163 and 1,671 bp long and encode 720 and 556 amino acid residues, respectively. A bifunctional fusion enzyme (SaMTSH) was constructed through the gene fusion of SaMTS and SaMTH. Recombinant SaMTS, SaMTH, and SaMTSH fusion enzyme were overexpressed in E. coli BL21. SaMTS and SaMTH produced trehalose and maltotriose from maltopentaose in a sequential reaction. SaMTSH fusion enzyme catalyzed the sequential reaction in which the formation of maltotriosyltrehalose was followed by hydrolysis leading to the synthesis of trehalose and maltotriose. The SaMTSH fusion enzyme showed the highest activity at pH 5.0-5.5 and $70-75^{\circ}C$. SaMTS, SaMTH, and SaMTSH fusion enzyme were active in soluble starch, which resulted in the production of trehalose.

• International journal of advanced smart convergence
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• 제10권3호
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• pp.214-224
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• 2021

We was confirmed that the antler extract increases the expression of marker genes expressed in the process of bone formation, and that the effect on the increase in the expression of the gene is further increased by fermentation of the antler extract. In addition, the mouse model in which menopausal was induced by ovary extraction significantly reduced the movement distance and exercise time of mice compared to the control group. But the decrease was somewhat alleviated by the administration of the antler extract, and completely restored when the fermented antler extract was administered. In the menopause-induced mice, the body weight ratio of heart, liver, and spleen weights increased compared to the control group, but the antler extract and the antler ferment extract restored the body weight ratio of various organ weights to the level of the control group in the menopause-induced mice. Consequently, this has led to mitigating changes in the metabolism affected by menopause.

• Journal of Ginseng Research
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• 제44권3호
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• pp.496-505
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• 2020

Background: Panax ginseng is a marvelous herbal remedy for all ailments of body. That may be why it is called Panax, which means "cure for all". Melanin is a pigment that gives color to our skin; however, increased melanin production can lead to tumor formation. Human exposure to ultraviolet B radiation has increased extensively owing to the increased sunlight due to global warming. Consequently, a phenomenon called photoaging has been observed for all skin colors and types. As a result of this phenomenon, a set of enzymes called matrix metalloproteinases, which serve as degradation enzymes for extracellular matrix proteins, mainly collagen, is increased, causing depletion of collagen and resulting in early wrinkle formation. Methods: Therefore, in our study, we used the murine melanoma cell line B16/F10 to study the inhibition of melanogenesis by Korean Red Ginseng (KRG) extract in vitro and HRM-2 hairless mice exposed to artificial ultraviolet B to examine the efficacy of KRG in vivo. We prepared a 3% red ginseng extract cream and evaluated its effects on human skin. Results: Our results demonstrated that KRG induced potent suppression of tyrosinase activity and melanin production in B16/F10 cells; moreover, it reduced the transcription and translation of components involved in the melanin production pathway. In the in vivo experiments, KRG potently suppressed the expression of matrix metalloproteinases, reduced wrinkle formation, and inhibited collagen degradation. On human skin, ginseng cream increased skin resilience and skin moisture and enhanced skin tone. Conclusion: Therefore, we conclude that KRG is an excellent skin whitening and antiaging product.

• 한국조경학회지
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• 제37권4호
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• pp.86-93
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• 2009

병산서원을 대상으로 강학공간이자 내부 공간의 핵심인 강당영역과 외부지향의 매개공간이며 유상공간인 만대루를 중심으로 경관구조를 살피고 그 구도적 경관특성을 원심적 외부경관과 구심적 내부경관적 성향으로 구분하여 경관 이미지와 선호 요인을 비교 분석한 본 연구의 주요 결과는 다음과 같다. 병산서원의 중심공간인 입교당은 표고 약 85m 지점에 입지하고 있으며 전면에 노출된 병산(屛山)의 같은 높이까지 약 365m 이격되어 있다. 경관 매개요소인 낙동강의 폭은 백사장을 포함하여 약 240m 정도를 보이며, 병산의 경사도는 평균 $50^{\circ}$가 넘어 상당히 위압적 앙시각(仰視角)으로 지각된다. 만대루 중심에서 보여지는 병산의 앙각은 $10.5^{\circ}$를 이루고 있으며, 좌측 병산 최고봉은 약 $16^{\circ}$ 정도의 각도로 조망되는 경관구조를 이루고 있다. 한편, 입교당을 중심으로 한 강당군으로의 내부지향적 조망경관에 대한 시각 이미지 평가에서는 깨끗한 1.98, 아름다운 2.16, 친근한 2.20, 질서있는 2.21, 정적인 2.81 등의 변인이 높은 평가를 받아 경관적으로 단아하며 아름다운 공간이미지를 구축하고 있다. 만대루를 투사하고 낙동강변을 건너 병산까지 펼쳐지는 외부지향경관에 대한 이미지에 있어서는 열린 1.70, 넓은 1.78, 아름다운 1.96, 조화된 1.81, 질서있는 1.86 등의 변인들이 매우 높은 평가를 받아 틀에 넣어 조직화됨으로써 오히려 자연과 합치된 아름다운 경관으로 평가되었다. 병산서원 내부지향 및 외부지향 공간의 시각적 이미지를 설명하는 인자는 특이성인자, 심미성인자, 개방성인자, 자연성인자 등 모두 4개의 인자군으로 공간이미지가 함축되어 분석되었고 전체 변량 중 이들 인자군의 설명력은 55.90%이다. 병산서원 전체경관에 대한 시각선호에 가장 영향을 높게 미치는 변인은 심미성인자 및 자연성인자로 밝혀져 전통적 공간의 조망계획시 특이성이나 개방성의 창출보다는 심미성을 바탕으로 한 적절한 경관 노출과 차폐를 통한 자연성 확보가 시각적 즐거움을 창출하는데 보다 긴요함을 발견하였다.

• 한국전통조경학회지
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• 제34권4호
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• pp.37-50
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• 2016

창옥병의 지명 오류에 대한 문제의식을 바탕으로 고지도, 옛시문, 옛그림 등의 문헌자료조사와 암각 및 조망특성에 대한 현장조사 그리고 인터뷰를 통해 창옥병의 위치를 비정하고, 이곳의 향유주체인 사암 박순과 그가 경영한 배견와 및 별업 이양정의 공간 구조와 정원적 면모를 고찰한 결과는 다음과 같다. 창옥병은 옥병서원과 일체감을 갖는 공간역으로 옛그림과 고지도 그리고 암각문의 존재로 볼 때 옥병서원 바로 앞의 석벽임을 추정할 수 있다. 이 지역과 현재 창옥병으로 알려진 오가리석벽과는 직선거리로 불과 약 460m거리에 위치하고 있어 광의의 창옥병 경관영향권으로 볼 수 있다. 시문에 등장하는 "창옥병급산금(蒼玉屛及散襟)"이란 표현에서도 산금대라 암각된 병풍바위가 바로 협의의 창옥병임이 유추된다. 또한 오가리석벽에서는 단 1점의 암각도 확인되지 않는 반면 옥병서원 전면부 암벽 도처에는 사암 박순이 명명하고 주문하여 새긴 11개소의 암각 중 8개의 경물명과 제영시가 필첩(筆帖)처럼 밀집되어 있음은 옥병서원 앞의 강벽(江壁)이 의심할 여지없는 창옥병인 것으로 비정된다. '수월정신송균절조(水月精神松筠節調)'암각은 창옥병의 향유주체였던 사암의 고매한 인격과 덕행을 함축하는 창옥병의 상징언어이자 이곳의 장소 요체(要諦)로, 기존 알려진 것과는 달리 우암 송시열의 필적으로 바로잡아야 한다. 한편 사암의 정원유적 창옥병은 왼쪽으로부터 산금대-수경대-청학대-백학대의 4개 대로 구성되어 있다. 산금대 후면에는 본제인 배견와를, 백학대 좌측의 기암석벽 위에는 별업 이양정을 조성하여 멀리 오가리석벽의 장관(壯觀)을 관망하는 조망구도를 갖도록 계획되었다. 사대(四臺)) 좌측 가장 높은 곳에 입지한 이양정은 사암의 별업이자 독서처이자 장구지소(杖屨之所)이며 그 앞 너럭바위에 존재하는 와준(窪樽)은 사암의 술풍류를 반영한 정원시설로 비정된다.

• International Journal of Advanced Culture Technology
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• 제7권4호
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• pp.125-136
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• 2019

The radish skin and radish greens are an edible part of the radish. But they are removed before eating the radish and used as a byproduct or an animal feed material because of their tough and rough texture. Melanin is a pigment that gives colour to our skin. But increased production of melanin can turn into benign or malignant tumours. These days due to global warming, the amount of Ultra violet (UVB) rays has been extensively increased with sunlight. Due to this, a phenomenon called exogenous photo aging is widely observed for all skin colour and types. As a result of this phenomenon, a set of enzymes called matrix metalloproteinases (MMP's) that serves as degradation enzymes for extracellular matrix proteins mainly collagen is increased, causing depletion in collagen and resulting in early wrinkles formation. Therefore in our study we used the murine melanoma cell line B16/F10 to study the melanogenesis inhibition by Heated radish extract (HRE) in vitro and we used HRM-2 hair less mice exposed to artificial UVB for checking the efficacy of Heated radish extract in vivo. Furthermore, we prepared a 3% Heated radish extract (HRE) cream and checked its effects on human skin. Our results have clearly demonstrated that Heated radish extract (HRE) have potently suppressed the tyrosinase activity and melanin production in B16/F10 cells. It had also reduced the expression of components involved in melanin production pathway both transcriptionally and transitionally. In in vivo studies, HRE had potently suppressed the expression of MMP's and reduced the wrinkle formation and inhibited collagen degradation. Moreover, on human skin, ginseng cream increased the resilience, skin moisture and enhanced the skin tone. Therefore in light of these findings, we conclude that HRE is an excellent skin whitening and antiaging product.

• 대한화장품학회지
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• 제43권4호
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• pp.329-335
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• 2017

Triterpenoid, saponin, 전분 등이 함유되어 있는 것으로 알려진 Adenophorae radix (A. radix)의 뿌리 추출물을 이용하여 각질형성세포의 분화와 피부장벽기능에 대한 연구를 수행하였다. A. radix의 뿌리 추출물은 CV-1 세포를 이용하여 $PPAR{\alpha}$ 발현을 살펴본 결과, Wy-14,643 $0.5-1.0{\mu}M$ 수준의 발현양을 나타내었다. 인체 각질형성 세포주(HaCaT)와 각질형성세포(nomal human keratinocyte)에 대한 각질형성능(cornified envelop formation, CE)은 대조군에 비해 통계적으로 유의한 증가를 보였다. HaCaT 세포에 A. radix의 뿌리 추출물 처리하였을 때, transglutaminase (TGase-1)의 유의적 증가를 보였다. A. radix의 뿌리 추출물을 함유한 간단한 화장품 제형을 약 2주간에 걸쳐 임상시험을 실시한 결과, TEWL의 유의적 감소와 수분량의 증가를 살펴볼 수 있었으며, 하박 내측에서 지질을 추출하여 세라마이드를 분석한 결과 통계적으로 유의한 증가를 관찰할 수 있었다. 이를 통하여 A. radix의 뿌리 추출물을 건조피부나 아토피 등의 피부질환과 관련된 질환의 예방 및 치료제로 사용될 수 있을 것이다.

• 한국식품영양학회지
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• 제29권3호
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• pp.307-312
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• 2016

This study was performed to investigate the effects of Acer tegmentosum Maxim. extract (ATE) on non-alcoholic fatty liver in Sprague Dawley (SD) rats. During oral administration of ATE, non-alcoholic fatty liver was induced by treatment with DL-ethionine. The lipid, total cholesterol (T-CHO) and malondialdehyde (MDA) in the liver tissue of ATE-fed rats showed lower levels, as compared to ATE-unfed rats. In ATE-fed rats, the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and ${\gamma}$-glutamyl transferase (GGT) were lower than the case of ATE-unfed rats. Oil red staining of the liver showed that the lipid deposits were decreased by feeding ATE. These results strongly indicated that ATE has positive effects of protection against non-alcoholic fatty liver formation.

• 한국컴퓨터정보학회논문지
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• 제24권4호
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• pp.129-136
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• 2019

The purpose of this study was to provide basic data necessary for the development of clothing that can improve the satisfaction of body shape of elderly men by examining subjective characteristics and characteristics of perception type of older men's body shape. Using the Q methodology, I investigated and analyzed the process of formation and main characteristics of subjectivity of various types of recognition that elderly men express about their body shape. There were four types of perception of body shape in elderly men: flat hip and bird legs skinny body, back bent and abdominal obese body, thick neck and upper body development body, forward cervical neck and protruding abdomen obese body. The actual body shape of elderly men was classified according to the bust, waist circumference, hip circumference, and body part angle. But recognition body shape of elderly men was classified by visual factors like the abdomen, the angle of the neck, the inclination of the back, the slope of the waist, and the degree of obesity. It is necessary to consider the trend of silver fashion which is increasing in demand. In addition, it is necessary to develop clothing patterns of comfortable fit through ergonomic study on the physical characteristics of older men. Consideration should be given to the design and pattern that can reduce the difference between the ideal body shape and the body shape recognized by older men.

• 패션비즈니스
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• 제27권5호
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• pp.78-92
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• 2023

Fashion trends are actively disseminated through social media, which influences both their propagation and consumption. This study explored how users perceive subversive basic fashion in social media videos, by examining the associated concepts and characteristics. In addition, the factors contributing to the style's social media dissemination were identified and its distinctive features were analyzed. Through text mining analysis, 80 keywords were selected for semantic network and CONCOR analysis. TF-IDF and N-gram results indicate that subversive basic fashion involves transformative design techniques such as cutting or layering garments, emphasizing the body with thin fabrics, and creating bold visual effects. Topic modeling suggests that this fashion forms a subculture that resists mainstream norms, seeking individuality by creatively transforming the existing garments. CONCOR analysis categorized the style into six groups: forward-thinking unconventional fashion, bold and unique style, creative reworking, item utilization and combination, pursuit of easy and convenient fashion, and contemporary sensibility. Consumer actions, linked to social media, were shown to involve easily transforming and pursuing personalized styles. Furthermore, creating new styles through the existing clothing is seen as an economic and creative activity that fosters network formation and interaction. This study is significant as it addresses language expression limitations and subjectivity issues in fashion image analysis, revealing factors contributing to content reproduction through user-perceived design concepts and social media-conveyed fashion characteristics.