• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.13-17
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• 2004

To improve transformation efficiency, the callus of Lilium lancifolium Thunb. were bombarded by particles coated with pIG 121 Hm which include NPT II and GUS genes, and then cocultivated with Agrobacterium tumefaciens EHA101 which contain pIG121Hm binary vector, carrying neomycin phosphotransferase (NPT II) and $\beta$-Glucuronidase (GUS) genes. Three days after cocultivation with Agrobacterium tumefaciens and particle bombardment, the callus clusters were transferred to MS medium containing 1mg/L 2,4-D, 0.1mg/L BAP, 100mg/L kanamycin and 200mg/L carbenicillin. Four weeks after transfer to the selection medium, GUS expression was detected and PCR analysis revealed the presence of NPT II fragment of the expected size (700 bp) in the transformed callus. The GUS expression from Agrobacterium-mediated transformants after particle bombardment increased to over 3-folds compared with that of callus cocultivated with Agrobacterium tumefaciens without particle bombardment. The callus clusters containing kanamycin resistant gene were transferred to MS medium containing 1mg/L NAA and 1mg/L BAP. Somatic embryos were developed in four weeks and microbulbs expressing GUS were formed.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.1 no.1
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• pp.29-46
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• 1991

The study was conducted with 611 Industrial Health officer for 2 years(1998 : 394person, 1989 : 217 person). The purpose of this study was to measure efficiency of the Industrial Health Officer course (use of questionaire) also, in the furure, we would like to make a standard on the management of Industrial Health Officer (software & Hardware) The result are as follows: 1. the response of satisfaction was revealed highly 51 % on the Curriculum. 2. The response of high qualities was revealed highly 92% in the content of textbook. 3. The response of practical program was revaled highly 67.7% in composition of curriculum. 4. The response of high-class instructor was revealed highly on the selection of instructor. 5. the response of a proper term was revealed almost trainees on the education term. 6. When industrial hygienist conducted their Health task, difficulty point, first, the lack of top manager on the health-management in industries, second, take a hold coccurently with another task third, not secure theirs status in industries. 7. almost of Industial Health Officer want to taken highly practical education program, a field of industrial ventilation, measurment of working enviroment. 8. They wanted to taken a practical program that they could conduct handling they-self measuring device, in order to enhance the effect of hygiene education. 9. They responsed size of ideal class that the number of Industrial Health Officer was less than 20 person.

• Journal of the Korean Society of Groundwater Environment
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• v.5 no.1
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• pp.30-36
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• 1998

For successful soil flushing process selection of appropriate flushing reagents is essential. Futhermore, obtaining operating parameters for site remediation application through various bench-scale tests is also important. In this research a series of organic acids (acetic, citric, oxalic, and succinic acids) were tested for flushing capability. Copper-contaminated natural soil was used as a test medium, and flushing experiments were performed with batch system. All the organic acids used did not provide effective flushing conditions at concentration of 1 mM. At the acid concentration of 50 and 100 mM copper was removed efficiently although 50 and 100 mM did not show any significant differences in removal efficiencies. Citric acid and oxalic acid removed copper more efficiently than the others, and especially, citric acid showed over 87% of removal efficiency of copper at near neutral pH of 5 to 7. Speciation of extracted copper using GEOCHEM simulation showed majority of extracted copper existed as complexed with organic acids and only small portion of organic acids were complexed with copper indicating promising application of soil flushing with organic acid to heavy metal-contaminated site remediation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.4
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• pp.281-285
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• 2005

Pod dehiscence (PD), defined as the opening of pods along both the dorsal and ventral sutures, causes the seed to shatter in the field before harvesting and results in loss of seed yields. However, breeding for resistance to PD is difficult due to the complicated genetic behavior and environmental interaction. The objective of the present research was to analyze the genetic behavior of PD for improving the breeding efficiency of resistance to PD in soybean. PD after oven-drying the sampled pod at $40^{\circ}C$ for 24 hours was the most reliable to predict the degree of PD tested in the field. Keunolkong, a dehiscent parent, was crossed with non-dehiscent parents, Sinpaldalkong and Iksan 10. Using their $F_1\;and\;F_2$ seeds, PD was measured after oven drying the pod at $40^{\circ}C$ for 24 hours. The gene conferring PD behaved in different manners depending on the genetic populations. In the Keunolkong$\times$Sinpaldalkong population, PD seemed to be governed by single major recessive gene and minor genes, while several genes were probably involved in the resistance to pod dehiscence in the Keunolkong$\times$Iksan 10 population. Heritability for PD estimated in F2 population showed over $90\%$ in the two populations. High heritability of PD indicated that selection for resistance to PD should be effective in a breeding program. In addition, genetic mapping of quantitative locus (QTL) for PD in both populations may reveal that genes conferring PD are population-specific.

• Journal of the Korean Society of Clothing and Textiles
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• v.41 no.1
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• pp.184-195
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• 2017

Baseball is a life sport that gives vitality to people. However, currently available leg guards do not have a variety of sizes and it is very difficult to purchase a leg guard that is suitable for the size of a specific consumer. Therefore, this study investigated the size system of a leg guard sold in the market and suggested a grading deviation suitable for the legs of Korean males. The results of the study were as follows. First, most brands were sold only in three size systems as children's, teenagers, and adults. Adult size systems were not subdivided. Second, Korean male legs were classified into Group 1 (large girth and height and lower body muscle) and Group 2 (small girth and height and low underbody muscle). Third, the size system is based on vertical items such as height and mid-thigh circumference. Each group produced 10 sizes. The smallest height or the largest height was also found to be about 4.5 to 5.0cm smaller or larger than the sample size for each group. The total length of the leg guard was about 2.5cm when the height was increased or decreased by one size. It was confirmed that the deviation of the circumference of the mid-thigh should be about 2.0cm larger or smaller than the sample size even if the height is the same. The deviation of the knee circumference and ankle circumference was smaller than the other circumference. In conclusion, the dimension combinations of the leg guard must be set differently to provide a leg guard with high size suitability according to leg type.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1304-1309
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• 2005

The production of therapeutic proteins for human diseases in plants results in many economic benefits, including reduced risk of animal virus contamination, high yields, and reduced production and storage costs. Human cytokines, interleukin-11 (hlL-11) and granulocyte-macrophage colony-stimulating factor (hGM-CSF), cDNAs were introduced into rice or tobacco, using either the maize ubiquitin promoter or the 35S promoter. The primary hIL-11 transgenic rice plants exhibited stunted growth and a sterile phenotype, whereas the hIL-11 transgenic tobacco plants did not. This suggests that hIL-11 expression in rice disrupts the normal growth and development of the plant. The regeneration efficiency of rice calli transformed with hGM-CSF was found to be approximately a quarter of that seen with the hIL-11, suggesting that hGM-CSF expression is more deleterious to the regeneration of rice calli than is hIL-11. However, the surviving hGM-CSF transgenic rice plants exhibited a normal phenotype of growth. Therefore, it appears that only those transgenic rice lines that expressed the human cytokines in small quantities were able to survive the selection process.

• Applied Biological Chemistry
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• v.48 no.4
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• pp.331-334
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• 2005

In this study, the effects of five extraction methods for raw and processed corns were compared with respect to the integrity, yields and quality of DNA extracted from them and the results were assessed by PCR analysis. From the comparison of five extraction methods, DNA integrity showed a similar pattern. Amounts of genomic DNA obtained from the five extraction methods varies from $0.25{\mu}g\;to\;234{\mu}g$ per 1 g sample. The DNA yield extracted with CTAB method and DNeasy Plant Maxi kit is greater than that obtained from other extraction methods. These results would be applicable for the selection of an adequate extraction method for specific samples.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.2
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• pp.37-45
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• 2015

Typically, water quality sampling takes place intermittently since sample collection and following analysis requires substantial cost and efforts. Therefore regression models (or rating curves) are often used to interpolate water quality data. LOADEST has nine regression models to estimate water quality data, and one regression model needs to be selected automatically or manually. The nine regression models in LOADEST and auto-selection by LOADEST were evaluated in the study. Suspended solids data were collected from forty-nine stations from the Water Information System of the Ministry of Environment. Suspended solid data from each station was divided into two groups for calibration and validation. Nash-Stucliffe efficiency (NSE) and coefficient of determination ($R_2$) were used to evaluate estimated suspended solid loads. The regression models numbered 1 and 3 in LOADEST provided higher NSE and $R_2$, compared to the other regression models. The regression modes numbered 2, 5, 6, 8, and 9 in LOADEST provided low NSE. In addition, the regression model selected by LOADEST did not necessarily provide better suspended solid estimations than the other regression models did.

• Journal of Internet Computing and Services
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• v.6 no.4
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• pp.21-35
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• 2005

Software product-lines is the software development paradigm to attain the rapid development of quality applications by customizing the reconfigurable components and composing them based on predefined software architectures. Recently various methodologies for the component-based product lines are proposed, but these don't provide the specific implementation techniques of the components in terms of variability resolution mechanism. In other hand, the several approaches to implement the component supporting the variabilities resolution are developed, but these don't define the systematic analysis and design method considering the variabilities from the initial phase. This paper proposes the integration of PLUS, the one of product line methodologies extending UML modeling, and component code generation technique in order to increase the efficiency of producing the specific product in the software product lines. In this paper, the component has the hierarchical architecture consisting of the implementation elements, and each implementation elements are implemented as XSLT scripts. The codes of the components are generated from the feature selection. Using the microwave oven product lines as case study, the development process for the reconfigurable components supporting the automatic variability resolution is described.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.7
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• pp.926-935
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• 2015

The efficiency of genome-wide association analysis (GWAS) depends on power of detection for quantitative trait loci (QTL) and precision for QTL mapping. In this study, three different strategies for GWAS were applied to detect QTL for carcass quality traits in the Korean cattle, Hanwoo; a linkage disequilibrium single locus regression method (LDRM), a combined linkage and linkage disequilibrium analysis (LDLA) and a $BayesC{\pi}$ approach. The phenotypes of 486 steers were collected for weaning weight (WWT), yearling weight (YWT), carcass weight (CWT), backfat thickness (BFT), longissimus dorsi muscle area, and marbling score (Marb). Also the genotype data for the steers and their sires were scored with the Illumina bovine 50K single nucleotide polymorphism (SNP) chips. For the two former GWAS methods, threshold values were set at false discovery rate <0.01 on a chromosome-wide level, while a cut-off threshold value was set in the latter model, such that the top five windows, each of which comprised 10 adjacent SNPs, were chosen with significant variation for the phenotype. Four major additive QTL from these three methods had high concordance found in 64.1 to 64.9Mb for Bos taurus autosome (BTA) 7 for WWT, 24.3 to 25.4Mb for BTA14 for CWT, 0.5 to 1.5Mb for BTA6 for BFT and 26.3 to 33.4Mb for BTA29 for BFT. Several candidate genes (i.e. glutamate receptor, ionotropic, ampa 1 [GRIA1], family with sequence similarity 110, member B [FAM110B], and thymocyte selection-associated high mobility group box [TOX]) may be identified close to these QTL. Our result suggests that the use of different linkage disequilibrium mapping approaches can provide more reliable chromosome regions to further pinpoint DNA makers or causative genes in these regions.