Browse > Article

Expression of Human Interleukin-ll and Granulocyte-Macrophage Colony-Stimulating Factor in Transgenic Plants  

LEE BO-YE (School of Life Sciences and Biotechnology, Korea University)
LEE JEONG-HYUN (School of Life Sciences and Biotechnology, Korea University)
YOON HOON-SEOK (School of Life Sciences and Biotechnology, Korea University)
KANG KYUNG HO (National Institute of Crop Science, Rural Development Administration)
KIM KYUNG-NAM (Department of Molecular Biology, Sejong University)
KIM JAE-HONG (School of Life Sciences and Biotechnology, Korea University)
KIM JU-KON (Division of Bioscience and Bioinformatics, Myongji University)
KIM JEONG-KOOK (School of Life Sciences and Biotechnology, Korea University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.6, 2005 , pp. 1304-1309 More about this Journal
Abstract
The production of therapeutic proteins for human diseases in plants results in many economic benefits, including reduced risk of animal virus contamination, high yields, and reduced production and storage costs. Human cytokines, interleukin-11 (hlL-11) and granulocyte-macrophage colony-stimulating factor (hGM-CSF), cDNAs were introduced into rice or tobacco, using either the maize ubiquitin promoter or the 35S promoter. The primary hIL-11 transgenic rice plants exhibited stunted growth and a sterile phenotype, whereas the hIL-11 transgenic tobacco plants did not. This suggests that hIL-11 expression in rice disrupts the normal growth and development of the plant. The regeneration efficiency of rice calli transformed with hGM-CSF was found to be approximately a quarter of that seen with the hIL-11, suggesting that hGM-CSF expression is more deleterious to the regeneration of rice calli than is hIL-11. However, the surviving hGM-CSF transgenic rice plants exhibited a normal phenotype of growth. Therefore, it appears that only those transgenic rice lines that expressed the human cytokines in small quantities were able to survive the selection process.
Keywords
Human cytokines; interleukin-11; GM-CSF; transgenic plant; rice; tobacco;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Daniell, H., S. J. Streatfield, and K. Wycoff. 2001. Medical molecular farming: Production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci. 6: 1360- 1385
2 Fischer, R., E. Stoger, S. Schillberg, P. Christou, and R. M. Twyman. 2004. Plant-based production of biopharmaceuticals. Curr. Opin. Plant Biol. 7: 152-158   DOI   ScienceOn
3 Harmegnies, D., X. M. Wang, P. Vandenbussche, A. Leon, P. Vusio, J. Grotzinger, Y. Jacques, E. Goormaghtigh, B. Devreese, and J. Content. 2003. Characterization of a potent human interleukin-11 agonist. 2003. Biochem. J. 375: 23- 32   DOI   ScienceOn
4 Menassa, R., W. Kennette, V. Nguyen, R. Rymerson, A. Jevnikar, and J. J. Brandle. 2004. Subcellular targeting of human interleukin-10 in plants. J. Biotechnol. 108: 179- 183   DOI   ScienceOn
5 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 15: 473-497   DOI
6 Shin, Y. J., S. H. Hong, T. H. Kwon, Y. S. Jang, and M. S. Yang. 2003. High level of expression of recombinant human granulocyte-macrophage colony-stimulating factor in transgenic rice cell suspension culture. Biotechnol. Bioeng. 82: 778-783   DOI   ScienceOn
7 Stoger, E., C. Vaquero, E. Torres, M. Sack, L. Nicholson, J. Drossard, S. Williams, D. Keen, Y. Perrin, P. Christou, and R. Fischer. 2000. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol. Biol. 42: 583-590   DOI   ScienceOn
8 Wong, G. G., J. S. Witek, P. A. Temple, K. M. Wilkens, A. C. Leary, D. P. Luxenberg, S. S. Jones, E. L. Brown, R. M. Kay, E. C. Orr, C. Shoemaker, D. W. Golde, R. J. Kaufman, R. M. Hewick, E. A .Wang, and S. C. Clark. 1985. Human GM-CSF: Molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science 228: 810-815   DOI   PUBMED
9 Yang, D. D., F. Guo, B. Liu, N. Huang, and S. C. Watkins. 2003. Expression and localization of human lysozyme in the endosperm of transgenic rice. Planta 216: 597-603
10 Barton, V. A., M. A. Hall, K. R. Hunson, and J. K. Heath. 2000. Interleukn-11 signals through the formation of a hexameric receptor complex. J. Biol. Chem. 275: 36197- 36203   DOI   ScienceOn
11 Holsters, M., D. de Waele, A. Depicker, E. Messens, M. Van Montagu, and J. Schell. 1978. Transfection and transformation of A. tumefaciens. Mol. Gen. Genet. 163: 181-187   DOI   ScienceOn
12 Lim, Y.-Y., S.-M. Park, Y.-S. Jang, M.-S. Yang, and D.-H. Kim. 2003. Production of a functional mouse interferon from recombinant Saccharomyces cerevisiae. J. Microbiol.Biotechnol. 13: 537-543
13 Cheon, B. Y., H. J. Kim, K. H. Oh, S. C. Bahn, J. H. Ahn, J. W. Choi, S. H. Ok, J. M. Bae, and J. S. Shin. 2004. Overexpression of human erythropoietin (EPO) affects plant morphologies: Retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis. Transgenic Res. 13: 541-549   DOI   ScienceOn
14 Kim, E., D. Kim, H. Y. Hwang, J. Yoon, Y. Yoon, and K. Baek. 2004. High-level expression of recombinant human interleukin-2 in Chinese Hamster Ovary cells using the expression system containing transcription terminator. J. Microbiol. Biotechnol. 14: 810-816
15 Kim, S. Y., C. H. Lee, K. J. Kim, and Y. S. Kim. 2001. Expression of the functional recombinant interleukin-16 in E. coli and mammalian cell lines. J. Microbiol. Biotechnol. 11: 234-241
16 Lee, H.-S., M.-Y. Jang, J.-H. Choi, Y.-Y. Lee, H.-B. Park, Y.-S. Lee, S.-W. Kim, and M.-J. Ahn. 2003. Proliferation, apoptosis, and telomerase activity in human cord blood CD34+ cells cultured with combinations of various cytokines. J. Microbiol. Biotechnol. 13: 422-428
17 Cantrell, M. A., D. Anderson, D. P. Cerretti, V. Price, K. McKereghan, R. J. Tushinski, D. Y. Mochizuki, A. Larsen, K. Grabstein, S. Gillis, and D. Cosman. 1985. Cloning, sequence, and expression of a human granulocyte macrophage colony-stimulating factor. Proc. Natl. Acad. Sci. USA 82: 6250-6254
18 Giddings, G., G. Allison, D. Brooks, and A. Carter. 2000. Transgenic plants as factories for biopharmaceuticals. Nat. Biotechnol.18: 1151-1155   DOI   ScienceOn
19 Sardana, R. K., Z. Alli, A. Dudani, E. Tackaberry, M. Panahi, M. Narayanan, P. Ganz, and I. Altosaar. 2002. Biological activity of human granulocyte-macrophage colony-stimulating factor is maintained in a fusion with seed glutelin peptide. Transgenic Res. 11: 521-531   DOI   ScienceOn
20 Schillberg, S., N. Emans, and R. Fischer. 2002. Antibody molecular farming in plants and plant cells. Phytochem. Rev. 1: 45-54   DOI   ScienceOn
21 Hoekema, A., P. J. Hooykaas, and R. A. Schilperoort. 1984. Transfer of the octopine T-DNA segment to plant cells mediated by different types of Agrobacterium tumor- or root-inducing plasmids: Generality of virulence systems. J. Bacteriol. 158: 383-385
22 Ditta, G., S. Stanfield, D. Corbin, and D. R. Helinski. 1980. Broad host range cloning system for gram negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc.Natl. Acad. Sci. USA 77: 7347-7351
23 Kim, N. S., K. H. Chang, B. S. Chung, S. H. Kim, J. H. Kim, and G. M. Lee. 2003. Characterization of humanized antibody produced by apoptosis-resistant CHO cells under sodium butyrate-induced condition. J. Microbiol. Biotechnol. 13: 926-936
24 Jang, I.-C., B. H. Nahm, and J.-K. Kim. 1999. Subcellular targeting of green fluorescent protein to plastids in transgenic rice plants provides a high-level expression system. Mol. Breed. 5: 453-461   DOI   ScienceOn
25 Du, X. and D. A. Williams. 1997. Interleukin-11; Review of molecular, cell biology, and clinical use. Blood 89: 3897- 3908
26 Paul, S. R., F. Bennett, J. A. Calvetti, K. Kelleher, C. R. Wood, R. M. Jr. O'Hara, A. C. Leary, B. Silbley, S. C. Clark, D. A. Williams, and Y. C. Yang. 1990. Molecular cloning of a cDNA encoding interleukin-11, a stromal cell-derived lymphopoietic and hematopoietic cytokine. Proc. Natl.Acad. Sci. USA 87: 7512-7516
27 Lee, S., J.-S. Jeon, K.-H. Jung, and G. An. 1999. Binary vectors for efficient transformation of rice. J. Plant Biol. 42: 310-316   DOI   ScienceOn
28 Zhang, B., Y. H. Yang, Y. M. Lin, Q. Rao, G. G. Zheng, and K. F. Wu. 2003. Expression and production of bioactive human interleukin-18 in transgenic tobacco plants. Biotechnol. Lett. 25: 1629-1635   DOI   ScienceOn
29 Dalton, R. and S. Diego. 2004. California edges towards farming drug-producing rice. Nature 428: 591
30 Gutierrez-Ortega, A., F. Avila-Moreno, L. J. Saucedo- Arias, C. Sanchez-Torres, and M. A. Gomez-Lim. 2004. Expression of a single-chain human interleukin-12 gene in transgenic tobacco plants and functional studies. Biotechnol. Bioeng. 85: 734-740   DOI   ScienceOn
31 Lee, F., T. Yokota, T. Otsuka, L. Gemmell, N. Larson, J. Luh, K. Arai, and D. Rennick. 1985. Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells. Proc. Natl. Acad. Sci. USA 82: 4360-4364