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Comparison of the Efficiency from Raw and Processed Corns by Five Different DNA Extraction Methods  

Lee, Hun-Hee (Team of Nutrition Evaluation, Korea Food and Drug Administration)
Song, Hee-Sung (Institute of Life Sciences & Resources, Kyung Hee University)
Kim, Jae-Hwan (Institute of Life Sciences & Resources, Kyung Hee University)
Lee, Woo-Young (Team of Nutrition Evaluation, Korea Food and Drug Administration)
Lee, Soon-Ho (Team of Nutrition Evaluation, Korea Food and Drug Administration)
Park, Sun-Hee (Team of Nutrition Evaluation, Korea Food and Drug Administration)
Park, Hye-Kyung (Team of Nutrition Evaluation, Korea Food and Drug Administration)
Kim, Hae-Yeong (Institute of Life Sciences & Resources, Kyung Hee University)
Publication Information
Applied Biological Chemistry / v.48, no.4, 2005 , pp. 331-334 More about this Journal
Abstract
In this study, the effects of five extraction methods for raw and processed corns were compared with respect to the integrity, yields and quality of DNA extracted from them and the results were assessed by PCR analysis. From the comparison of five extraction methods, DNA integrity showed a similar pattern. Amounts of genomic DNA obtained from the five extraction methods varies from $0.25{\mu}g\;to\;234{\mu}g$ per 1 g sample. The DNA yield extracted with CTAB method and DNeasy Plant Maxi kit is greater than that obtained from other extraction methods. These results would be applicable for the selection of an adequate extraction method for specific samples.
Keywords
DNA extraction; GM Maize; PCR;
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