Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of Human Interleukin-ll and Granulocyte-Macrophage Colony-Stimulating Factor in Transgenic Plants

  • LEE BO-YE (School of Life Sciences and Biotechnology, Korea University) ;
  • LEE JEONG-HYUN (School of Life Sciences and Biotechnology, Korea University) ;
  • YOON HOON-SEOK (School of Life Sciences and Biotechnology, Korea University) ;
  • KANG KYUNG HO (National Institute of Crop Science, Rural Development Administration) ;
  • KIM KYUNG-NAM (Department of Molecular Biology, Sejong University) ;
  • KIM JAE-HONG (School of Life Sciences and Biotechnology, Korea University) ;
  • KIM JU-KON (Division of Bioscience and Bioinformatics, Myongji University) ;
  • KIM JEONG-KOOK (School of Life Sciences and Biotechnology, Korea University)
  • Published : 2005.12.01
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Abstract

The production of therapeutic proteins for human diseases in plants results in many economic benefits, including reduced risk of animal virus contamination, high yields, and reduced production and storage costs. Human cytokines, interleukin-11 (hlL-11) and granulocyte-macrophage colony-stimulating factor (hGM-CSF), cDNAs were introduced into rice or tobacco, using either the maize ubiquitin promoter or the 35S promoter. The primary hIL-11 transgenic rice plants exhibited stunted growth and a sterile phenotype, whereas the hIL-11 transgenic tobacco plants did not. This suggests that hIL-11 expression in rice disrupts the normal growth and development of the plant. The regeneration efficiency of rice calli transformed with hGM-CSF was found to be approximately a quarter of that seen with the hIL-11, suggesting that hGM-CSF expression is more deleterious to the regeneration of rice calli than is hIL-11. However, the surviving hGM-CSF transgenic rice plants exhibited a normal phenotype of growth. Therefore, it appears that only those transgenic rice lines that expressed the human cytokines in small quantities were able to survive the selection process.

Keywords

References

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