• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.111-118
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• 2011

For the strain improvement of the industrial polyploid yeast strain through hybridization and protoplast fusion, a dominant selection marker other than a recessive marker such as the auxotrophic marker was required for the selection of the resulting hybrids. In the present investigation, the aureobasidin A resistant gene was tested in relation to whether it can be used as the dominant selectable marker for the isolation of hybrids of the yeast Saccharomyces. The plasmid pAUR112, carrying the gene responsible for resistance to aureobasidin A, was introduced into the haploid yeast strain K114/YIp. From the rare-mating between polyploid C6 and haploid K114/YIp carrying pAUR112, many hybrids were obtained from the agar medium containing 0.5 ${\mu}g$/ml of aureobasidin A. The hybrids exhibited characteristics derived from both of the parental strains; and the cell sizes of the hybrids were larger than those of the parental strains. These results showed that the aureobasidin A resistant gene could be successfully used as the dominant selectable marker for the isolation of yeast hybrids resulting from rare-mating.

• Journal of Plant Biotechnology
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• v.50
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• pp.63-69
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• 2023

This study includes the transformation of genes such as ORE7, the increase of gene expression, and the use of the bar gene as a selectable marker that shows herbicide resistance with Agrobacterium tumefaciens using hypocotyls from the oilseed rape "Youngsan" cultivar. To establish an Agrobacterium transformation system for the production of oilseed rape with a high-yield trait, infection time and co-cultivation period with Agrobacterium were tested. Therefore, when hypocotyls from the oilseed rape "Youngsan" cultivar were infected with Agrobacterium for 20 min and co-cultivated for 3 days, approximately 32-36 putatively transformed hypocotyls with shoots including roots survived from 100 inoculated hypocotyls after 4 weeks of transformation on a selection medium containing 20 mg/L of phosphinothricin (PPT) as a selectable agent. Additionally, a PCR assay was performed to confirm the insertion of target genes and showed the presence of the ORE7 gene as a high-yielding trait and the bar gene as a selectable marker. Treatment with 0.5% (v/v) Basta solution as a selectable agent for 6 days with leaves from transformed oilseed rape expressed the bar gene. Therefore, this study can contribute to the development of special oilseed rapes containing agriculturally useful traits such as herbicide resistance, drought tolerance, high yielding traits, and high oleic acid content.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2007.11a
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• pp.285-286
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• 2007

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.10a
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• pp.413-414
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• 2009

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2012.10a
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• pp.185-186
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• 2012

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.134-134
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• 2003

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2010.05a
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• pp.395-396
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• 2010

• Journal of the Korean Institute of Telematics and Electronics C
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• v.36C no.7
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• pp.17-26
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• 1999

In this paper, Bit selectable and Bi-directional Interface Device is described, which can communicate data with the peripheral devices. Specially, an algorithm of truth-table comparison that synthesizes the pulse-type sequential circuit pulse has been proposed to design the Interrupt Generator, and implemented in designing the Interrupt Register. Also, a description of the asynchronous design method is given to remove the clock skew phenomenon, and the output asynchronous control method which finds the optimal clock and controls all the enable signal of the output pins at the same time is presented. Using this technique interface ports have delay time of less-than 0.7ns.

• Journal of Biosystems Engineering
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• v.31 no.1 s.114
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• pp.39-45
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• 2006

Fundamental informations on performance of sets of solenoid valve and nozzle were necessary to develop a target selectable spraying system. The experiments were performed for the sets of solenoid valves and flat spraying nozzles at 3 levels of the operating pressures, and the obtained results are as follows; Rise time of the system pressure to reach up to 90% of the operating pressure after the valve turned was affected by nozzle size and operating pressure. Maximum overshoot of the system pressure was about 170% not affected by the sets of valve and nozzle and operating pressure. The system pressure after its settle down within 5% of the set pressure showed good uniformity in any condition of the experiment as less as 2.3% of its coefficient of variation. Time requirement to lower the system pressure down to 50% after the valve power turned off was affected by valve and nozzle set and operating pressure.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.15 no.2
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• pp.140-144
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• 2004

The selectable dual band LNA usually uses common source transistor pair each input of which is selectively driven at a different frequency in a series resonant form. This paper analyzes the degradation in noise figures of the MOSFET common source pair with series resonance when it is driven concurrently at both inputs with different frequencies as a concurrent dual band LNA. Results of analysis will be compared with the measured noise figures of CMOS LNA with double inputs fabricated in 0.18 $\mu\textrm{m}$ CMOS process. Additionally, analyzing the contributions of FET channel noise and source noise from the LNA operating in the other band, this paper proposes optimum matching topology which minimizes the added noises for concurrent operation.