• Journal of fish pathology
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• v.24 no.3
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• pp.297-305
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• 2011

A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.

• Korean Journal of Microbiology
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• v.30 no.2
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• pp.83-87
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• 1992

When the transposon is used as a selectable marker. the cotransduction frequency depends on the selection order of the markers. In this study. we adopted a mathematical approach to explain this phenomenon. At first, the formation of transducing particles were considered in five different configurations. Then the probability functions indicating the possibilities for a marker to be fixed were mathematically formulated on the basis of probability density concept. After actual values and useful assumptions were integrated into the Formula. resulting frequencies from theoretical calculations were compared with actual data. Such analysis let us conclude that the asymmetry in the frequency arose from the lack of homology required for homologous recombination due to the transposon insertion and from the suppression of recombination around the region where the transposon is inserted.

• Fashion & Textile Research Journal
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• v.8 no.4
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• pp.420-426
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• 2006

Korean prior studies on smart clothing seeking complete mixture of clothing and computer with diverse functions in the ubiquitous environment were so mere, while there have been aggressive activities to develop diverse smart clothing products led by the relevant industries and universities in foreign countries. Given prospective growth of the smart clothing market, there should be customers' evaluation of functions and utility of and sensitivity to smart clothing as well. This study conducted a questionnaire to Korean customers in their twenties regarding their perception and liking of and willingness to use smart clothing, and examined the needs of new functions of smart clothing. As a result, the customers in their twenties turned out to like well-designed and comfortable clothing, and be exposed to diverse and continuous computing environment. Also, they have relatively low perception but considerably high liking of smart clothing, indicating their high willingness to use smart clothing products. They cited 'selectable functions' and 'mobile and convenient ' as merits, and 'too expensive' and 'easy to wash' as demerits of smart clothing. In the respect, they proposed many interesting smart clothing ideas, such as 'clothing with automatic temperature-sensing function, which gets higher heat- insulating function by tightening the textile structure in winter and higher ventilating function by loosening the textile structure in summer.' We hope the results of this study will serve basic data for development of more efficient smart clothing in the future.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.44 no.8
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• pp.60-64
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• 2007

In this paper, we designed FFT for European DTV and implemented system with Stratix EP1S25F672C6 FPGA At the implemented FFT, we used SIC architecture. SIC architecture is composed of algorithm-specific processing element, RAM memory, registers, and a central or distributed control unit. Designed FFT was acceptable either 2K or 8K point FFT processing, and is selectable guard interval such as 1/4, 1/8, 1/16, 1/32. Consequently, it was suitable for the standard of DVB-T(Digital Terrestrial Video Transmission System) specification. It resulted in 12% of total logic gate and 53% of total memory bit in Stratix device.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.7 no.3
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• pp.254-262
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• 1996

In this paper, we present a polarizer that consists of three step rotary detents which can selectively convert linear polarzation into circular polarization and vice versa. For the design of the polarizer, the transmission line theory is applied to design the wa- veguide mode transducer for the modes to be smothly converted in waveguides, and a dielectric plate is inserted in circular waveguide for the conversion of a polarized wave with the angle of an inserted dielectric plate. Also, we simulated to obtain the optimum values of the transmission and the reflection coefficient characteristics at input and output port, and proved the propriety of the theory from the knowledge of measuring the constructed polarizer with the designed data.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.9 no.6
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• pp.63-68
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• 2009

In the whole information technology area, the protection of information from hacking or tapping becomes a very serious issue. Therefore, the more effective, convenient and secure methods are required to make the safe operation. Encryption algorithms are known to be computationally intensive. They consume a significant amount of computing resources such as CPU time and memory. In this paper we propose the scalable encryption scheme with four criteria, the compression of plaintext, variable size of block, selectable round and software optimization. We have tested our scheme by c++. Experimental results show that our scheme achieves the faster execution speed of encryption/decryption.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.25 no.9
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• pp.879-885
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• 2014

In this paper, we designed and fabricated wideband 3-axis isotropic antenna for the Electro-Magnetic Fields(EMFs) measurement. Each part of proposed 3-axis antenna has isotropic characteristics and arbitrary axis of proposed 3-axis antenna could be selectable using RF switch. Also, a resistor was inserted in each axis of proposed 3-axis antenna for improving antenna gain and noise suppression characteristics, and port impedance of each dipole antenna were matched by balun. For the performance verification of antenna, GTEM Cell which generates standard electromagnetic field was used for the derivation of antenna factor and receiver sensitivity. As a result, fabricated 3-axis isotropic antenna has receiver sensitivity of 0.12~4.2 mV/m and typical VSWR of 3.3:1 within a wide operation frequency range from 0.03 MHz to 3 GHz.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.31-36
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• 2007

Though higher plants car not metabolize D-amino acid, many prokaryotes and eukaryotes have the D-amino acid metabolism. Therefore, we transformed tobacco plants with D-amino acid oxidase (DAO), which can metabolize D-amino acid, and confirmed that transgenic tobacco plants might metabolize D-amino acid. Transgenic tobacco plants were survived a high concentration of D-serine, however non-transgenic plants were not grown on D-serine medium. From Southern and Northern blot analysis, transgenic tobacco plants selected on D-serine medium were confirmed by insert and expression of transgene. $T_{1}$ tobacco seeds derived $T_{0}$ tobacco plants selfing were grown on D-serine medium and showed normal phenotype compared to wild tobacco plants. Transgenic tobacco plants displayed the metabolic capability of D-serine. Therefore, we suggested that DAO is useful selectable marker gene for plant transformation.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.40 no.6
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• pp.61-70
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• 2003

In this paper, a transcoding algorithm for the Selectable Mode Vocoder (SMV) and the G.723.1 speech coder via direct parameter transformation is proposed. In contrast to the conventional tandem transcoding algorithm, the proposed algorithm converts the parameters of one coder to the other without going through the decoding and encoding process. The proposed algorithm is composed of four parts: the parameter decoding, line spectral pair (LSP) conversion, pitch period conversion, excitation conversion and rate selection. The evaluation results show that the proposed algorithm achieves equivalent speech quality to that of tandem transcoding with reduced computational complexity and delay.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.323-330
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• 2019

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.