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http://dx.doi.org/10.5010/JPB.2007.34.1.031

D-amino Acid Oxidase (DAO) Gene as a Novel Selection Marker for Plant Transformation  

Lim, Sun-Hyung (National Institute of Agricultural Biotechnology, RDA)
Woo, Hee-Jong (National Institute of Agricultural Biotechnology, RDA)
Lee, Si-Myung (National Institute of Agricultural Biotechnology, RDA)
Jin, Yong-Moon (National Institute of Agricultural Biotechnology, RDA)
Cho, Hyun-Suk (National Institute of Agricultural Biotechnology, RDA)
Publication Information
Journal of Plant Biotechnology / v.34, no.1, 2007 , pp. 31-36 More about this Journal
Abstract
Though higher plants car not metabolize D-amino acid, many prokaryotes and eukaryotes have the D-amino acid metabolism. Therefore, we transformed tobacco plants with D-amino acid oxidase (DAO), which can metabolize D-amino acid, and confirmed that transgenic tobacco plants might metabolize D-amino acid. Transgenic tobacco plants were survived a high concentration of D-serine, however non-transgenic plants were not grown on D-serine medium. From Southern and Northern blot analysis, transgenic tobacco plants selected on D-serine medium were confirmed by insert and expression of transgene. $T_{1}$ tobacco seeds derived $T_{0}$ tobacco plants selfing were grown on D-serine medium and showed normal phenotype compared to wild tobacco plants. Transgenic tobacco plants displayed the metabolic capability of D-serine. Therefore, we suggested that DAO is useful selectable marker gene for plant transformation.
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