• Title/Summary/Keyword: Seaweed alginate

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Preparation of Oligosaccharides from Alginic Acid by Enzymic Hydrolysis (효소분해에 의한 알긴산 올리고당류의 제조)

  • Joo, Dong-Sik;Lee, Jung-Suck;Park, Jung-Je;Cho, Soon-Yeong;Kim, Hee-Kyung;Lee, Eung-Ho
    • Korean Journal of Food Science and Technology
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    • v.28 no.1
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    • pp.146-151
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    • 1996
  • For the purpose of production of oligosaccharides from alginates, a bacterium was isolated from seaweed, and then an enzyme which degraded alginates was obtained from the bacterium. A specific activity of the enzyme was shown in G-rich block and Na-alginate (Wako Co.) as a result of reaction between the enzyme and six types of alginates (G-rich block, M-rich block and 4 commercial Na-alginate). Degradation products were prepared from the Na-alginate (Wako Co.) by the enzyme. The oligosaccharides were fractioned by Sephadex G-25 and Bio-gel P-2 and identified on a thin layer chromatography (TLC). Degree of polymerization (DP) of the oligosaccharides was shown from 2.6 to 7.5.

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Effects of Water-Soluble Polysaccharides from Tott on Lipid Absorption and Animal Body Weight (톳 유래 수용성 다당류의 Lipase 저해활성과 지질 및 체중변화에 미치는 영향)

  • Baek, Gu;Goo, Bon Geun;Ahn, Byung Jae;Park, Jae Kweon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.4
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    • pp.556-562
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    • 2013
  • We examined the anti-obesity effects of water-soluble polysaccharides (WSP-A) extracted from the seaweed Hijikia fusiforme (Tott in Korean). The extracted alginate-like polysaccharide (verified by FT-IR and HPAEC-PAD analysis) was examined in a lipase inhibition assay and animal experiments. WSP-A inhibited lipase up to 30%, with over 80% of the initial activity retained until the 1 hour reaction in vitro. There was a 30% loss in the rate of weight gain in rats fed a high-fat diet. WSP-A therefore seems to serve as a healthy weight loss agent by inhibiting lipases, thus preventing the absorption of fat in the body.

Isolation of Seaweed Hydrolytic Strains from Microfloras in Rice field Ditch Water (논두렁 물의 미생물군으로부터 해조분해능을 갖는 균주의 분리)

  • KIM Hae-Sub;CHOI Ok-Soo;KANHG Dong-Soo;PARK Uk-Min;BAEK Seung-Han;BAE Tae-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.6
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    • pp.563-567
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    • 2003
  • Various bacterial strains were isolated from rice field ditch water, and their seaweed degrading activities were investigated. They were incubated in a liquid medium of sea tangle (Laminaria iaponica) and sea mustard (Undaria pinnatifida) powder for 3 weeks. Ratios of reduced sugar to total sugar of the liquid medium were measured once a week. Ratios of reduced sugar to total sugar of 27A311, 27C221 27A111 and 27B121 strains were highest. Accordingly, these four strains were incubated in 3 different liquid media of sodium alginate, sea tangle powder, and sea mustard powder for 3 or 4 weeks. The ratios of reduced sugar to total sugar and cell growth were measured once a week. Cell growth was higher in 27A111 and 27C221. Ratios of reduced sugar to total sugar was higher for 27C221 in the liquid mediums with sodium alginate and sea mustard powder, and for 27A111 in the liquid medium with sea tangle powder.

Comparison of Alginic Acid Yields and Viscosity by Different Extraction Conditions from Various Seaweeds (Laminaria religiosa, Hizikia fusiforme, and Undaria pinnatifida) (추출조건에 따른 해조류의 알긴산 수율과 점도)

  • 윤미옥;이승철;임종환;김정목
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.747-752
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    • 2004
  • Alginates were extracted from the Laminaria religiosa, Undaria pinnatifida, and Hizikia fusiforme by using four different extraction methods and compared the yields of alginate. Acid-alkali soluble alginate (AASA) extraction method from Undaria pinnatifida resulted in the best yield of alginate among the seaweeds. The optimal condition for extracting alginate from Laminaria religiosa was 0.4 N H$_2$SO$_4$ and 3% NaCO$_3$ concentrations at the AASA extraction method. The alginate yields of hot water extractable material (HWEM) water soluble alginate (WSA), alkali soluble alginate (ASA) and AASA in Hizikia fusiforme were 18.6, 4.7, 22.5 and 26.5%, respectively. The alginates manufactured by the WSA extraction method showed more bright color than those of the ASA and AASA extraction methods. The alginate prepared by the ASA extraction method from Hizikia fusiforme showed the higher viscosity than that of the ASA extraction method. The molecular weight of the alginate from Hizikia fusiforme was 33.3 kDa to 121.6 kDa depending on the extraction method.

Microbial Modification of Extracellular Polysaccharides

  • Jin Woo Lee
    • Journal of Life Science
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    • v.9 no.1
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    • pp.69-80
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    • 1999
  • Some trials to alter the structure of extracellular polysaccharides by means of biotransformation and microbial modification have been reported. Seaweed alginate was acetylated by intact and resting cells of Pseudomonas syringae ATCC 19304. Glucose analogs such as 3-O-methyl-D-glucose used as sole carbon sources was directly incorporated into curdlan by agrobacterium sp. ATCC 31749. The 2-amino-2-deoxy-D-glucose (glucosamine)and 2-acetamido-2-deoxy-D-glucose (N-acetylglucosamine) were incorporated into microbial cellulose by Acetobacter xylinum ATCC 10245. The changed monomeric composition in pullulan by Aureobasidium pullulans ATCC 42023 as well as zooglan by Zoogoea ramigera ATCC 25935 was another effect of glucose analogs used a carbon source. There was no effect of glucose analogs found in polysacharide-7 (PS-7) produced by Beijerinckia indica. ATCC 21423.

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Biosorption of Metal Ions by Seaweed Alginate, Polyguluronate, and Polymannuronate (알긴산, 폴리글루론산 및 폴리만뉴론산에 의한 금속이온의 흡착)

  • Jung, Dae-Young;Son, Chang-Woo;Kim, Sung-Koo;Kim, Yi-Joon;Chung, Chung-Han;Lee, Jin-Woo
    • Journal of Life Science
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    • v.19 no.5
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    • pp.553-560
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    • 2009
  • Based on $P_{1/2}$ values, relative affinities of alginate, polyguluronate, and polymannuronate for metal ions are, in order, as follows; 1) seaweed alginate: $Cu^{2+}$ > $Cd^{2+}$ > $Pb^{2+}$ > $Fe^{3+}$ >> $Zn^{2+}$ > $Sr^{2+}$ > $Ca^{2+}$ > $Co^{2+}$ >> $Cr^{6+}$ > $Mn^{2+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$, 2) polyguluronate: $Cd^{2+}$ > $Cu^{2+}$ > $Pb^{2+}$ > $Fe^{3+}$ >> $Ca^{2+}$ > $Sr^{2+}$, $Zn^{2+}$, $Co^{2+}$ >> $Mn^{2+}$ > $Cr^{6+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$, and 3) polymannuronate: $Cd^{2+}$, $Cu^{2+}$ > $Fe^{3+}$ > $Pb^{2+}$ > $Ca^{2+}$ > $Zn^{2+}$ > $Sr^{2+}$ > $Co^{2+}$ > $Cr^{6+}$ >> $Mn^{2+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$. Amounts of the metal ions, $Cd^{2+}$, $Cu^{2+}$, $Fe^{3+}$, $Pb^{2+}$, and $Zn^{2+}$, bound to 1 g of seaweed alginate, were measured as $363.5{\pm}45.0$, $226.3{\pm}9.2$, $1,299.4{\pm}$81.3, 500.7${\pm}$27.7, and 165.9${\pm}$11.4 mg, respectively. Amounts of the metal ions, $Cd^{2+}$, $Cu^{2+}$, $Fe^{3+}$, $Pb^{2+}$, and $Zn^{2+}$, bound to 1g of polyguluronate, were 354.5${\pm}$26.5, 177.6${\pm}$8.7, 1,288.6${\pm}$60.1, 424.0${\pm}$7.4, and 140.2${\pm}$28.5 mg, respectively, whereas those bound to 1 g of polymannuronate were 329.0${\pm}$10.3, 206.9${\pm}$1.9, 1,635.6${\pm}$11.1, 419.8${\pm}$12.6, and 251.0${\pm}$49.1 mg, respectively. Due to its higher solubility than alginate and higher affinity for metal ions than polyguluronate, polymannuronate can be used for bioremediation or biosorption of toxic and/or noble metal ions.

Development of Value-Added Products Using Seaweeds (해조류 가공식품 및 부산물을 이용한 제품 개발)

  • Park, Yang-Kyun;Kang, Seong-Gook;Jung, Soon-Teck;Kim, Dong-Han;Kim, Seon-Jae;Pak, Jae-In;Kim, Chang-Hyeug;Rhim, Jong-Whan;Kim, Jung-Mook
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.3
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    • pp.133-141
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    • 2007
  • There are produced more than 600,000 tons of seaweeds every year along the coast of the Korea. Jeonnam province, south-west coast area, of Korea is producing 93% of total amounts of seaweeds. The laver, sea mustard, and tangleweed maintain stability in the output and has been exported as a simple product processing through drying or salting. It was evaluated the low value-added products and limited the expansion for the consumption of seaweeds. The seaweeds contains 40-60% carbohydrate and structurally different compared with land plant. The dietary fiber from seaweeds has been known the function of facilitating the bowl movement, excretion the heavy metal in the body, lowering the blood cholesterol level, anti-coagulant of blood, and anticancer. Especially, brown algae including sea mustard, seaweed fusiforme, and tangleweed contains alginic acid, laminarin, mannitol, fucoidan which are lowering the blood cholesterol level, lowering blood pressure, and fusion of blood clot. Agar-agar, carrageenan, and porphyran compound in red algae are known to antimutagenicity and anticoagulant function. In spite of potential of seaweed as a main bio-resource, there are lack of research to facilitate the consumption with its functional properties and consumers are unsatisfied with simple processing products. Also, the seaweed by-product dump into the sea and cause pollution of the seawater. Therefore, there are needed the scheme to promote the consumption of seaweeds. The development of value-added products, finding functional properties from seaweeds, development the functional feed for animal using seaweed by-products, and utilization of unused algae for food or other industrial uses will increase fisherman's income as well as serve as an aid for the people health due to its functional properties. Using by-product of seaweed and unexploited seaweed are needed to development of bio-degradable food packaging material and functional feed for animal.

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Enhanced GABA content from sodium alginate-induced Sparassis latifolia influences dendrite development in primary cortical neurons (해조류 기반 엘리시터 처리에 의한 꽃송이버섯의 GABA 함량 증가 및 흥분성 신경세포의 수상돌기 발달 억제)

  • Choi, Moon-Hee;Ki, SungHwan;Lee, Seong-Eun;Lee, GumHwa;Shin, Hyun-Jae
    • Journal of Mushroom
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    • v.17 no.4
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    • pp.275-283
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    • 2019
  • Sparassis latifolia is a fungus abundant in β-glucan and amino acids and is highly valued as a medicinal mushroom. Among amino acids, γ-aminobutyric acid (GABA) is a free amino acid and has biological effects, such as increase/decrease of hypertension, improvement of cerebral blood flow, and prevention of dementia. In this study, biological elicitors were used to increase bioactive substances as a biofortification method. Sodium alginate extracted from seaweed (Sargassum horneri, Sargassum fulvellum, Sargassum fusiforme) were used as the elicitor. The levels of β-glucan and GABA in the mycelium and fruiting body grown by adding the elicitor to the medium were investigated. Addition of sodium alginate positively affected GABA production and negatively affected the β-glucan production in these fungi. Sodium alginates extracted from S. fulvellum induced the highest increase in GABA in the mycelium and fruiting bodies. Moreover, we investigated the effects of the extracts from mycelium and fruiting bodies on dendrite development in primary cortical neurons. We found that the extract from the fruiting bodies of sodium alginate treated fungi with increased levels of GABA inhibited the dendrite outgrowth of excitatory neurons, but not inhibitory neurons.

Elucidation of Anti-Tumor Initiator and Promoter Derived from Seaweed-4: Desmutagenic Principles of Ecklonia stolonifera Extracts against Carcinogenic Heterocyclic Amines (해조류 중의 Anti-Tumor Initiator 및 Promoter의 해석-4: 발암성 Heterocyclic Amine에 대한 곰피 추출물 중의 돌연변이원성 억제인자)

  • 김선봉;박영범;안종관;유승재;박덕천;김인수;박영호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.3
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    • pp.537-542
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    • 1998
  • The present study was performed to elucidate desmutagenic principles from Ecklonia stolonifera extracts against 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine(PhIP) and 2-amino-3,8-dime-thylimidazo[4,5-f]duinoxaline(MeIQx) with Salmonella/mammalian-microsome mutagenicity test. Alginate, phenols, chlorophyll and carotenoids from Ecklonia stolonifera were extracted and their desmutagenicities were assayed. Alginate hydroysates showed desmutagenic activities against PhIP and MeIQx at high level dose. Phenol fractions and bromophenol showed desmutagenic activity of about MeIQx at high level dose. Phenol fractions and bromophenol showed desmutagenic activity of about 90% per 0.5mg against PhIP and MeIQx. Chlorophyllin among chlorophyll derivatives exhibited remarkable desmutagenic activities of 92.9% and 82.7% at 20uM against PhIP and MeIQx, respectively. Carotenoids, such as lutein and $\alpha$-cryptoxanthin isolated from Ecklonia stolonifera exerted also high desmutagenic activity. Major desmutagenic substances from Ecklonia stolonifera are considered to be chlorophyllin, phenols, lutein, $\alpha$-cryptoxanthin and low molecular alginates.

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Determination of Sodium Alginate in Processed Food Products Distributed in Korea

  • Yang, Hyo-Jin;Seo, Eunbin;Yun, Choong-In;Kim, Young-Jun
    • Journal of Food Hygiene and Safety
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    • v.36 no.6
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    • pp.474-480
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    • 2021
  • Sodium alginate is the sodium salt of alginic acid, commonly used as a food additive for stabilizing, thickening, and emulsifying properties. A relatively simple and universal analysis method is used to study sodium alginate due to the complex pretreatment process and extended analysis time required during the quantitative method. As for the equipment, HPLC-UVD and Unison US-Phenyl column were used for analysis. For the pretreatment condition, a shaking apparatus was used for extraction at 150 rpm for 180 minutes at room temperature. The calibration curve made from the standard sodium alginate solution in 5 concentration ranges showed that the linearity (R2) is 0.9999 on average. LOD and LOQ showed 3.96 mg/kg and 12.0 mg/kg, respectively. Furthermore, the average intraday and inter-day accuracy (%) and precision (RSD%) were 98.47-103.74% and 1.69-3.08% for seaweed jelly noodle samples and 99.95-105.76% and 0.59-3.63% for sherbet samples, respectively. The relative uncertainty value was appropriate for the CODEX standard with 1.5-7.9%. To evaluate the applicability of the method developed in this study, the sodium alginate concentrations of 103 products were quantified. The result showed that the detection rate is highest from starch vermicelli and instant fried noodles to sugar processed products.