• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.213-225
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• 2016

To reduce attrition in drug development, it is crucial to consider the development and implementation of translational phenotypic assays as well as decipher diverse molecular mechanisms of action for new molecular entities. High-throughput fluorescence and confocal microscopes with advanced analysis software have simplified the simultaneous identification and quantification of various cellular processes through what is now referred to as high-content screening (HCS). HCS permits automated identification of modifiers of accessible and biologically relevant targets and can thus be used to detect gene interactions or identify toxic pathways of drug candidates to improve drug discovery and development processes. In this review, we summarize several HCS-compatible, biochemical, and molecular biology-driven assays, including immunohistochemistry, RNAi, reporter gene assay, CRISPR-Cas9 system, and protein-protein interactions to assess a variety of cellular processes, including proliferation, morphological changes, protein expression, localization, post-translational modifications, and protein-protein interactions. These cell-based assay methods can be applied to not only 2D cell culture but also 3D cell culture systems in a high-throughput manner.

• Animal cells and systems
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• 제7권2호
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• pp.133-137
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• 2003

Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast-based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.

• 한국미생물생명공학회소식지:생물산업
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• 제12권3호
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• pp.45-49
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• 1999

• 대한의생명과학회지
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• 제12권1호
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• pp.35-42
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• 2006

Alloimmunization to red blood cell (RBC) antigens may cause a delayed hemolytic transfusion reactions (DHTR) and a delayed serologic transfusion reactions (DSTR). In the present study, the frequency of alloimmunization and its clinical significance were evaluated. Also, transfusions were correlated with antibody formation. Alloimmunization rate was 0.63%. Alloimmunization rate in multiple transfused patients was 24.5%. The most common clinically significant alloantibodies of alloimmunized patients were found to be Rh antibodies (52.6%). Nine patients out of 38 (23.7%) became undetectable after the first detection. To be positive at antibody screening test after RBC transfusion was mean transfused numbers: 3.7 units, mean transfused periods: 56 days, mean transfused frequencies: 1.7 times. The results from antibody specificity and RBC transfusions were comparatively analyzed and it shows that Rh system antibodies were longer than other antibodies (P<0.05). In case of disease group, malignant diseases was longer than other diseases (P<0.05). In order to prevent the formation of RBC alloimmunization, irregular antibody screening tests were performed at propriety intervals in multiple transfused patients.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3521-3526
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• 2016

Digital breast tomosynthesis (DBT) as a breast cancer screening modality, through generation of three-dimensional images during standard mammographic compression, can reduce interference from breast tissue overlap, increasing conspicuity of invasive cancers while concomitantly reducing false-positive results. We here conducted a systematic review on previous studies to synthesize the evidence of DBT efficacy, eventually 18 articles being included in the analysis. The most commonly emerging topics were advantages of DBT screening tool in terms of recall rates, cancer detection rates and cost-effectiveness, preventing unnecessary burdens on women and the healthcare system. Further research is needed to evaluate the potential impact of DBT on longer-term outcomes, such as interval cancer rates and mortality, to better understand the broader clinical and economic implications of its adoption.

• 품질경영학회지
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• 제25권4호
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• pp.57-70
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• 1997

Consider a production system where all manufactured products are screened through automatic inspection equipment. The products for which the measured value of quality characteristic is larger than the lower screening specification limit (SL) and smaller than the u, pp.r screeing specification limit(SU) are accepted. Those smaller than SL are reworked and those larger than SU are excluded from the process. Assuming that the quality characteristic is normally distributed with known variance, this paper suggests cost models which involve inspection cost, production cost, rework cost, exclusion cost and quality cost, and presents the methods of finding the optimal values of the screening specification limits and the process target. Numerical example is given to demonstrate the a, pp.icability of the cost models suggested in this paper.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1988년도 전기.전자공학 학술대회 논문집
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• pp.879-882
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• 1988

This paper describes a fast security analysis techique for voltage security assessment. The new method identifies the location of buses with potential voltage problems and thereby defines a voltage-sensitive subnetwork for contingency screening. The efficieny of this method is derived from the use of a voltage subnetwork to drastically reduce the number of bus voltages to be solved; and subsequently from the use of compensation techniques and sparse-vectors methods for screening. Results demonstrating the effectiveness of the method on the IEEE-14 bus model system are presented.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 추계학술대회 논문집 전력기술부문
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• pp.73-76
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• 2001

This paper presents a new systematic contingency selection, screening and ranking method for on-line transient security assessment. Transient stability of a particular generator is influenced most by fault near it. Fault at the transmission lines adjacent to the generators are selected as contingency. Two screening methods are developed using the sensitivity of modal synchronizing torque coefficient and computing an approximate critical clearing time(CCT) without time simulation. The first method, which considers only synchronizing power, may mislead in some cases since it does not consider the acceleration power. The approximate CCT method, which consider both the acceleration and deceleration power, worked well. Finally the Single Machine Equivalent(SIME) method is implemented using IPLAN of PSS/E for detailed stability analysis.

• 산업경영시스템학회지
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• 제35권4호
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• pp.1-9
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• 2012

Decisions on reliability screening rules and burn-in policies are determined based on the estimated reliability. The variability in a semiconductor manufacturing process does not only causes quality problems but it also makes reliability estimation more complicated. This study investigates the nonuniformity characteristics of integrated circuit reliability according to defect density distribution within a wafer and between wafers then develops optimal burn-in policy based on the estimated reliability. New reliability estimation model based on yield information is developed using a spatial stochastic process. Spatial defect density variation is reflected in the reliability estimation, and the defect densities of each die location are considered as input variables of the burn-in optimization. Reliability screening and optimal burn-in policy subject to the burn-in cost minimization is examined, and numerical experiments are conducted.

• 전자통신동향분석
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• 제34권2호
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• pp.73-82
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• 2019

Airport is shifted airport 1.0 to airport 4.0 called smart airport and services paradigm is changed into direction to point the customer targeted benefits. Smart airports make use of integrated Internet of Things components to provide added-value services. By integrating smart components, airports are being exposed to a larger attack surface and new attack vectors. Self-services such as web or mobile check-in, self check-in/tagging/back drop/boarding, etc. should be strengthened to make airport processes smarter, and technologies such as automatic immigration, smart security search, and automatic AI-based baggage search should be applied. In this paper, we describe the necessity and importance of smart airports and next generation security screening technology. Further, we describe a walk through-type smart security screening system.