• Title/Summary/Keyword: Scavenging System

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Scavenge of superoxide and hydrogen peroxide by bovine intact red blood cells (한우 무손상 적혈구의 superoxide 및 과산화수소 제거능력)

  • Cho, Jong-hoo;Park, Sang-youel
    • Korean Journal of Veterinary Research
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    • v.38 no.2
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    • pp.273-279
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    • 1998
  • The ability of bovine intact red blood cells to scavenge superoxide and hydrogen peroxide by superoxide dismutase, catalase and glutathione peroxidase was investigated. Intact red cells(up to 0.4%) suspensions did not inhibit ferricytochrome c reduction by superoxide in the superoxide generating system. On the other hand, intact red cell(0.4%) suspensions almost completely inhibit ferrocytochrome c oxidation by hydrogen peroxide. The ability of intact red cells to scavenge hydrogen peroxide was mainly attributed to either membrane bound catalase or glutathione peroxidase. The scavenge of hydrogen peroxide by 0.1~0.2% intact red cells showed a trend of dependence on mainly glutathione peroxidase. However, at blood cell concentration higher than 0.3%, the process depended upon peroxidase-independent scavengers like catalase. Enhancement of ferrocytochrome c oxidation by red cells treated with aminotriazole proved that the protection against hydrogen peroxide was due to catalase, while the protection in the presence of glutathione indicated scavenging effect of glutathione peroxidase against hydrogen peroxide.

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Anti-inflammatory and Immunomodulatory Effects of the Botanical Product $AMP-365^{TM}$ (천연물제제 마루플랜트$-AMP-365^{TM}$의 항염증 및 면역활성에 미치는 영향)

  • Shin, Eun-Myoung;Kim, Dong-Hyun;Kwon, Young-Bok;Kim, Yeong-Shik
    • Korean Journal of Pharmacognosy
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    • v.37 no.3
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    • pp.212-216
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    • 2006
  • The effect of aqueous extracts of medicinal plants $AMP-365^{TM}$ was tested for immune system regulating activity based on anti-inflammatory activity, anti-oxidant, macrophage proliferation and T-lymphocyte proliferation activity. $AMP-365^{TM}$ dose-dependently increased proliferation of RAW264.7 macrophage cells and its nitric oxide production as well as DPPH radical scavenging activity. On the other hand, T-lymphocyte proliferation activity was decreased on dose-dependent manner. Passive cutaneous anaphylaxis reaction was alleviated by 49% by administering 250 mg/kg of $AMP-365^{TM}$. The results suggest that $AMP-365^{TM}$ can be beneficial in the treatment of immediate allergic reactions as an adjuvant supplement material.

A STUDY ON THE PERFORMANCE OF KHAKI CAMPBELL, DESI AND KHAKI CAMPBELL X DESI DUCKS WITH AND WITHOUT EXTRA FEEDING UNDER RURAL CONDITION OF BANGLADESH

  • Rashid, N.;Barua, A.;Bulbul, S.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.4
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    • pp.375-378
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    • 1995
  • Three month old 600 ducklings of Khaki Campbell/KC Desi and KC X Desi were reared for a period of 13 months in the villages surrounding Bangladesh Agricultural University in order to study the economic traits of duck with and without extra feed to scavenging system of rearing. KC attained earlier sexual maturity followed by KC X Desi and Desi ducks and crossbred showed closeness to sire breed for this trait, KC X Desi found heavier and produce more eggs than their parental breeds. KC showed higher hatchability followed by KC X Desi and Desi ducks were more resistant followed by KC X Desi and KC to diseases. Extra feeding were found more beneficial to ducks irrespective of breed and types.

Schisandra Chinensis Inhibits Oxidative DNA Damage and Lipid Peroxidation Via Antioxidant Activity

  • Jeong, Jin-Boo;Jeong, Hyung-Jin
    • Korean Journal of Plant Resources
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    • v.22 no.3
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    • pp.195-202
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    • 2009
  • Schisandra chinensis have been traditionally used in Asia for the treatment of dyspnea, cough, mouth dryness, spontaneous diaphoresis, nocturnal diaphoresis, nocturnal emission, dysentery, insomnia and amnesia. The purpose of this study is to evaluate the protective effects of Schisandra chinensis on oxidative DNA damage and lipid peroxidation induced by ROS in non cellular and cellular system. DPPH radical, hydroxyl radical and hydrogen peroxide scavenging assay were used to measure the antioxidant activities. Phi X-174RF I plasmid DNA cleavage assay and intracellular DNA migration assay were used to evaluate the protective effect on oxidative DNA damage. MTT assay and lipid peroxidation assay were used for evaluating the protective effect on oxidative cell damage. It was found to scavenge DPPH radical, hydrogen peroxide and hydroxyl radical and it inhibited oxidative DNA damage, lipid peroxidation and cell death induced by hydroxyl radical. These data indicate that Schisandra chinensis possesses a spectrum of antioxidant and DNA-protective properties

Measurement on Vibration Mode of Tire Wheel as a Energy Source of Micro Power (초소형 동력 에너지원으로서의 타이어 휠 진동 모드 측정)

  • Shin, You-Hwan;Lee, Yoon-Pyo
    • Transactions of the Korean hydrogen and new energy society
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    • v.20 no.1
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    • pp.64-72
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    • 2009
  • In order to convert efficiently vibration energy of a car tire wheel into electrical power by using piezoelectric materials, the design of the materials must be performed for the dynamic characteristics of the piezoelectric materials to be matched with them of the vibration energy sources well. An accelerometer was installed on the tire wheel with a slip ring to investigate the dynamic mode of the wheel as one of the vibration energy sources. During road test, the measurement on the vibration mode of the tire wheel was performed with variations of car speed and road condition. The experimental data were analyzed details for availability as a micro power source for wireless TPMS(Tire Pressure Monitoring System).

Antioxidative Activity of Rice Grain using FI-CL and ESR Methods

  • Chung, Ill-Min;Kim, Kwang-Ho;Ahn, Joung-Kuk;Lee, Si-Kyung;Lee, Jin-Ohk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.5
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    • pp.411-415
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    • 2001
  • Fifty-four Korean native and 28 foreign rice varieties harvested in 1998 and 1999 were examined for antioxidative activity that is measured to a chemiluminescence and superoxide radical intensity, by the flow injection chemiluminescence (FI-CL) system and an electron spin resonance (ESR) spectrophotometer, respectively. In the chemiluminescence measurement by FI-CL, radical scavenger activity did not differ significantly among rice varieties between origin types of rice varieties, and between storage periods. GinSun and Hongchoengdo, colored rice exhibited high electron scavenging effect by ESR. Therefore, these results indicate that the pigments of rice varieties may play important antioxidative roles and that it may be possible to breed rice varieties with higher antioxidative potentials.

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Antioxidant Activity of Ethanolic Extract of Potato(Solanum tuberlosum) (감자(Solanum tuberlosum) 에탄올 추출물의 황산화 활성)

  • Choi Eun-Mi;Koo Sung-Ja
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.5
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    • pp.479-486
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    • 2004
  • 감자는 전세계적으로 섭취되는 주요 작물로서 우리나라에서는 감자의 생즙을 관절염 및 통증을 억제하는 민간요법으로 사용되어 왔다. 본 연구에서는 감자 에탄을 추출물의 항산화 작용을 검토하였다. 감자 에탄올 추출물(0.05~5 mg/mL) 은 1,1-diphenyl-2-picrylhydrazyl radical에 대하여 강한 free radical scavenger 활성을 나타내었다. 또한 감자 추출물(0.5 mg/mL)은 쥐간 균질액 에서 생성 된 TBARS의 양을 유의적으로 억제하였으며 (p<0.05) Fe/sup 2+//ascorbate induction system에서 감자 추출물(0.5~2 mg/mL)은 DNPH 반응으로 측정된 carbonyl 형성을 억제하였다. 항산화 활성과 마찬가지로 감자 추출물의 환원력은 0.5 mg/mL 농도에서 가장 높았고 이는 α-tocopherol의 경우와 비슷하였다. 이 결과는 감자 추출물이 항산화력을 통해 생체에서 작용할 수 있는 가능성을 제시한다.

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An Analysis on Charateristics of Separate Oiling to Reduce Oil Consumption for a 2 Stroke Free-Piston H2 Engine (오일 소모 저감을 위한 역단류 2행정 프리피스톤 수소기관의 분리 윤활 특성 해석)

  • Byun, Cahng-Hee;Baek, Dae-Ha;Lee, Jong-Tae
    • Transactions of the Korean hydrogen and new energy society
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    • v.22 no.6
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    • pp.794-799
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    • 2011
  • In order to reduce the oil consumption for a 2 stroke free piston hydrogen fueled engine, the behaviors of residual lubricant oil of the cylinder wall surface were visualized and oil mass emitted into exhaust port was measured by using research engine with cross-head and eccentric crankshaft. As the results, it was shown that characteristics of residual lubricant oil such as oil thickness and distribution were remarkably different from a conventional 4 stroke engine. It was also analyzed that these tendencies relied on the configuration and installed position of the exhaust port, piston pin boss and so on.

Metallothionein Induces Site-specific Cleavages in tRNAPhe

  • Seon, Jung-Yun;Koh, Moon-Joo
    • Bulletin of the Korean Chemical Society
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    • v.26 no.6
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    • pp.921-924
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    • 2005
  • It is known that metallothionein (MT) plays a role in the scavenging of free radicals, which is produced under various stress conditions. MT may function as an antioxidant that protects against oxidative damage of DNA, protein, and lipid induced by superoxide anion, hydrogen peroxide, hydroxyl radical, nitric oxide, and peroxynitrite. This study was undertaken to test the hypothesis that MT also protects from RNA damage induced by peroxynitrite, an important reactive nitrogen species that causes a diversity of pathological processes. A cell-free system was used. RNA damage was detected by the mobility of $tRNA^{Phe}$ in electrophoresis. Cleavages on tRNA were not induced by 3-morpholinosydnomine, which produces peroxynitrite directly. MT induced tRNA damage which was site specific.

Antioxidant effects of the Root of Vitis labrusca Water and Aqua-acupuncture Solution (포도근 열수 추출액 및 약침액이 항산화에 미치는 효과)

  • Lim Seong-Cheorl;Moon Jin-Young
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.1
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    • pp.76-82
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    • 2006
  • This study was purposed to investigated the antioxidant effects of water (VLWS) and aqua-acupuncture solution (VLAS) from root of Vitis labrusca. VLWS and VLAS was were assessed to determine the mechanisms of its antioxidant activities. The VLWS and VLAS exhibitde a concentration-dependent inhibition of DPPH free radicals. This VLWS-mediated antioxidant activity was similar to that L-ascorbic acid (vitamin C). In addition, the VLWS and VLAS showed dose-dependent free radical scavenging activity, including superoxide anions and hydroxyl radicals, using different assay systems. The VLWS and VLAS were also found to be strongly inhibited linoleic acid oxidation in a ferric thiocyanate assay (FTC) system. Finally, the VLWS and VLAS significantly prevented Fenton's reagent-induced DNA nicking.