Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antioxidant effects of the Root of Vitis labrusca Water and Aqua-acupuncture Solution

포도근 열수 추출액 및 약침액이 항산화에 미치는 효과

  • Lim Seong-Cheorl (Cardiovascular Medical Research Center and Dept. of AM-Pointology, College of Oriental Medicine, Dongguk University) ;
  • Moon Jin-Young (Cardiovascular Medical Research Center and Dept. of AM-Pointology, College of Oriental Medicine, Dongguk University)
  • 임성철 (심혈관계질환 연구.센터 동국대학교 한의과대학 경혈학교실) ;
  • 문진영 (심혈관계질환 연구.센터 동국대학교 한의과대학 경혈학교실)
  • Published : 2006.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was purposed to investigated the antioxidant effects of water (VLWS) and aqua-acupuncture solution (VLAS) from root of Vitis labrusca. VLWS and VLAS was were assessed to determine the mechanisms of its antioxidant activities. The VLWS and VLAS exhibitde a concentration-dependent inhibition of DPPH free radicals. This VLWS-mediated antioxidant activity was similar to that L-ascorbic acid (vitamin C). In addition, the VLWS and VLAS showed dose-dependent free radical scavenging activity, including superoxide anions and hydroxyl radicals, using different assay systems. The VLWS and VLAS were also found to be strongly inhibited linoleic acid oxidation in a ferric thiocyanate assay (FTC) system. Finally, the VLWS and VLAS significantly prevented Fenton's reagent-induced DNA nicking.

Keywords

References

  1. Barnen, A.L. Toxicological and biochemistry of BHA, BHT. J. Am. Oil Chem. Soc. 52, 59-64, 1975 https://doi.org/10.1007/BF02901825
  2. Chung, I.M., Kim, K.H and Ahn, J.K. Screening of Korean medicinal and food plants with antioxidant activity. Kor. J. of Med. Sci. 6, 311-322, 1998
  3. Hofeman, D.G. and Hoekstra, W.G. Protein against carbon tetrachloride induced lipid peroxidation in the rat by dietary vitamin E selenium and methionine as measured by ethan evolution. J. Nutr. 107, 667-670, 1977
  4. Yagi, K. Lipid peroxides and human disease. Chem. Phys. Lipids. 45, 337, 1987 https://doi.org/10.1016/0009-3084(87)90071-5
  5. Bishov, S.J., Masuoka, Y. and Kapsalis, J.G. Antioxidant effect of spices herbs and hydrolyzates in freeze dried model system synergistic action with synthtic antioxidants. J. Food Proc. Preserv. 1, 153-159, 1977 https://doi.org/10.1111/j.1745-4549.1977.tb00320.x
  6. Chang, S.S., Biserka, O.M., Olive, A.L. and Huang, C.L. Natural antioxidants from rosemary and sage. J. Food Sci. 42, 1102-1107, 1977 https://doi.org/10.1111/j.1365-2621.1977.tb12676.x
  7. Fukuda, Y., Osawa T., Kawagishi, S. and Namiki, H. Oxdative stability of foods fried with sesame oil. N. S. K. G. 38, 28-30, 1988
  8. Kim, S.W., Kim, E.S. and Kim, Y.S Studies on the polysaccharide extracted from Ganderma Lucidum. J. Kor. Soc. Food Sci. Natr. 24, 147-150, 1995
  9. Kim, Y.J., Kim, C.K. and Kwon, Y.J. Isolation of antioxidative components of Perillae semen. Kor. J. Food Sci. Technol. 29, 38-44, 1997
  10. Naom, M., Gestetner, B., Bondi, A. and Birk, Y. Antioxidantive and antihemolytie of sobean isoflavones. J. Agric. Food Chem. 24, 1174-1176, 1976 https://doi.org/10.1021/jf60208a029
  11. Lee, J.C., Kim, H.R., Kim, J. and Jang, Y.S. Antioxidant property of ethanol extract of the stem of Opuntia ficus- indica var. Saboten. J. Agric. Food Chem. 50, 6490-6496, 2002 https://doi.org/10.1021/jf020388c
  12. Lin, C.C., Wu, S.J., Chang, C.H. and Ng, L.T. Antioxidant activity of Cinnamomum cassia. Phytother. Res. 17, 726-730, 2003 https://doi.org/10.1002/ptr.1190
  13. Wei, Z., Bai, O., Richardson, J.S., Mousseau, D.D. and Li, X.M. Olanzapine protects PC12 cells from oxidative stress induced by hydrogen peroxide. J. of Neuro. Res. 73, 364-368, 2003 https://doi.org/10.1002/jnr.10668
  14. Rohrdanz, E., Bittner, A., Taran-Thi Q. and Kahl, R. The effect of quercetin on the mRNA expression of different antioxidant enzymes in hepatoma cells. Arch. Toxicol. 77, 506-510, 2003 https://doi.org/10.1007/s00204-003-0482-7
  15. 許 浚 : 東醫寶鑑, 서울, 법인문화사, p 3448-3449, 2005
  16. Marie-Veronique, C., Jayshreekumari, L.H., Sanaul-Haq, C., and Shazib, P. Chemopreventive agent resveratrol, a natural product from grapes, triggers CD95 signaling-dependent apoptosis in human tumor cells. Blood. 3, 996-1002, 1998
  17. Kai-Sheng, H., Mao, L. and Gui-Fang, C. Anti-inflammatory tetramers of resveratrol from the roots of Vitis amurensis and the conformations of the seven-membered ring in some oligostilbenes. Phytochemistry. 58, 357-362, 2001 https://doi.org/10.1016/S0031-9422(01)00224-2
  18. Yu-Ling, H., Wei-Jern, T., Chien-Chang, S. and Chien-Chih, C. Resveratrol derivatives from the roots of Vitis thunbergii. J. Nat. Prod. 68, 217-220, 2005 https://doi.org/10.1021/np049686p
  19. 錢百炎 : 中草藥主射劑, 上海, 上海科學技術出版社. pp 71-132, 1981
  20. Gyamfi, M.A., Yonamine, M. and Aniya, Y. Free-radical scavenging action of medicinal herbs from Ghana. Gen. Pharmacol. 32, 661-667, 1999 https://doi.org/10.1016/S0306-3623(98)00238-9
  21. Gotoh, N. and Niki, E. Rates of interactions of superoxide with vitamin E, vitamin C and related compounds as measured by chemiluminescence. Biochim. Biophys. Acta. 1115, 201-207, 1992 https://doi.org/10.1016/0304-4165(92)90054-X
  22. Halliwell, B., Gutteridge. J.M.C. and Aruoma, O.I. The deoxyribose method: simple "test-tube" assay for determination of rate constants for reaction of hydroxyl radicals. Anal. Biochem. 165, 215-219, 1987 https://doi.org/10.1016/0003-2697(87)90222-3
  23. Osawa, T., and Namiki, M. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agric. Biolog. Chem. 45, 735-740, 1981 https://doi.org/10.1271/bbb1961.45.735
  24. 沈吉浩 : 老化의 槪念과 豫防, 서울, 成文出版社, pp 15-17, 1987
  25. Ames, B.N., Cahcart, R., Schwiers, E. and Hochstein, P. Uric acid provides an antioxidant defense in humans against oxidant and radical-caused aging and cancer. A Hypothesis. Proc. Natl. Acad. Sci. 78, 6858-6862, 1981
  26. Aruoma, O.I., Kaur, H. and Halliwell, B. Oxygen free radicals and human diease. J. Roy. Soc. Health. 111, 172-177, 1991 https://doi.org/10.1177/146642409111100506
  27. Pendurthi U.R., Williams J.T. and Rao, L.V. Resveratrol, a polyphenolic compound found in wine, inhibits tissue factor expression in vascular cells : A possible mechanism for the cardiovascular benefits associated with moderate consumption of wine. Arterioscler Thromb. Vasc. Biol. 19, 419-426, 1999 https://doi.org/10.1161/01.ATV.19.2.419
  28. Philip M. The use of the stable free radical diphenylricrylhydrazyl (DPPH) for estimating antioxidant. Songklanakarin J. Sci. Technol. 26, 211-219, 2004
  29. Abdalla, A.E and Roozen, J.P. Effect of plant extracts on the oxidative stability of sunflower oil and emulsion. Food Chem. 64, 323-329, 1999 https://doi.org/10.1016/S0308-8146(98)00112-5
  30. 大柳善疹 : SOD よ活性酸素調節濟よの 藥理作用 よ 臨床應用, 東京, 日本醫學館, p 9, 1989