• IMMUNE NETWORK
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• 제6권3호
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• pp.117-122
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• 2006

Background: Caveolin, a family of integral membrane proteins are a principal component of caveolae membranes. In this study, we investigated the effect of p38 kinase on differentiation and on inflammatory responses in sodium nitroprusside (SNP)-treated chondrocytes. Methods: Rabbit articular chondrocytes were prepared from cartilage slices of 2-week-old New Zealand white rabbits by enzymatic digestion. SNP was used as a nitric oxide (NO) donor. In this experiments measuring SNP dose response, primary chondrocytes were treated with various concentrations of SNP for 24h. The time course of the SNP response was determined by incubating cells with 1mM SNP for the indicated time period $(0{\sim}24h)$. The cyclooxygenase-2 (COX-2) and type II collagen expression levels were determined by immunoblot analysis, and prostaglandin $E_2\;(PGE_2)$ assay was used to measure the COX-2 activity. The tyrosine phosphorylation of caveolin-1 was determined by immunoblot analysis and immunostaining. Results: SNP treatment stimulated tyrosine phosphorylation of caveolin-1 and activation of p38 kinase. SNP additionally caused dedifferentiation and inflammatory response. We showed previously that SNP treatment stimulated activation of p38 kinase and ERK-1/-2. Inhibition of p38 kinase with SB203580 reduced caveolin-1 tyrosine phosphorylation and COX-2 expression but enhanced dedifferentiation, whereas inhibition of ERK with PD98059 did not affect caveolin-1 tyrosine phosphorylation levels, suggesting that ERK at least is not related to dedifferentiation and COX-2 expression through caveolin-1 tyrosine phosphorylation. Conclusion: Our results indicate that SNP in articular chondrocytes stimulates dedifferentiation and inflammatory response via p38 kinase signaling in association with caveolin-1 phosphorylation.

• KIEAE Journal
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• 제15권5호
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• pp.47-57
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• 2015

Purpose: The purpose of this study is to set the type of 'the New-hanok type Public Buildings' through a case study for the hanok public buildings completed after 2000 years, and to analyze planned properties of the type. This is significant Establishing legal status of 'the New-hanok type Public Buildings' and seeing review of application possibilities of the type for providing a systematic government support measures of 'the New-hanok type public buildings' when models developing future. Method: Method of research is the first to examine the current laws and established the definition and legal status of 'the New-hanok type Public Buildings'. Followed by Setting the type classification criteria as to classify the type of 'the New-hanok type public buildings' and research architectural overview of selected cases by Literature, Internet searches, etc. After systematizing of the types classification of analysis cases, Characteristics of the type of the building structure looks catch classify in spatial structure, function, beauty. Finally, review application possibilities of the type for systematic government support measures establish when models developing of 'the New-hanok type Public Buildings' through a comprehensive analysis. Result: Selected cases were categorized as 3 types according by structural standard based on the core concept of 'the New-hanok type Public Buildings' set in this study. This can be divided into 'Wooden Structure type' and 'Composite structure - Convergence type' and 'Composite structure - juxtaposed type', 'Wooden Structure type' was re-classified by divided into '(1)Traditional Korean Wooden Structure' and '(2)Laminated Wood Wooden Structure'.

• 한국수산과학회지
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• 제36권5호
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• pp.528-534
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• 2003

The 2-D hydraulic experimental results for the submerged rubble-mound structure, we have been concerned with the slability/function characteristics of the structures by the effects of wave force, scour/deposition at the toe and the wave transmission ratio at the lee-side sea. So, to investigate the variation characteristics of the wave transmission ratio which depended on a geometrical structure of the submerged breakwater profiles, the critical conditions for the depth of submergence and crest width were obviously presented. In summary, the results lead us to the conclusion that the wave control capabilities of submerged breakwaters by the variation of the submergence depth is higher than about 4 times the degree at the efficiency than the that of crest width. The destruction of the covering block at the crest generated at the region which was located between the maximum and minimum damage curve, and it's maximum damage/failure station from the toe of the structure was $0.2\;L_s.$ As the wave transmission coefficient and the slope of the structure increase, the damage/failure ratio and the maximum scour depth at the toe was extended, respectively. When the maximum scour depth happened, the destruction of the covering block which was located at the toe generated at the front of the submerged rubble-mound breakwater. Finally, it was found from the results that the optimization of the structure may be obtained by the efficient decision of the submergence depth and crest width in the permissible range of the wave transmission ratio.

• 한국미생물·생명공학회지
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• 제20권2호
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• pp.169-177
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• 1992

토양으로부터 alkaline protease 생성능이 강한 Streptomyces griseus HC-1141을 분리하였으며, 효소생산의 최적 배양조건은 0.5 casein, 0.05 ammonium chloride, 0.1 ferrous sulfate, 2.0의 lactose, pH 8.0에서 84시간 배양했을 때이다. 효소의 정제는 ammonium sulfate 침전, DEAE-cellulose ion exchange chromatography, Sephadex G-150 gel filtration, crystallization으로 하여 53.23배 정제할 수 있었으며 polyacrylamide gel 전기영동상 단일밴드를 나타내었다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2009년도 춘계 학술발표회
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• pp.579-584
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• 2009

Arrangement of the facilities for improving harbor functions depends on sea and land conditions such as the ship's arrival and departure conditions, waves and tide. And the plan and the size of the facilities depend much on harbor and marine environment condition such as cargo quantity, ship size, ship traffic and seawater circulation. Among these, waves have so much effect on a breakwater design that it is the most important to understand their characteristics and to apply them to breakwater design. Therefore, to analyze the effect of waves characteristics over a rubble mound breakwater, we have calculated wave pressure by using numerical analysis at each tide level and have analyzed the effect of wave pressure on structure stability by conducting the stability analysis with the wave pressure. As a result, it is found that during low and mean tide level time the biggest wave pressure is estimated near calm water level. But during high tide time, the biggest wave pressure is estimated in front of capping. And the stability analysis indicates also that a structure is most unstable when low tide time wave pressure is acting on. After reviewing the stability of a structure by applying vertical and horizon wave forces, it is concluded that safety factor is lower than ordinary time(max. about 15%), is also reviewed when designing a rubble mound breakwater.

• Bulletin of the Korean Chemical Society
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• 제24권1호
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• pp.49-54
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• 2003

TiO₂nanoparticles were synthesized using the metallorganic chemical vapor deposition process. Particles with and without metal ion dopants were obtained. X-ray photoelectron and energy dispersive X-ray spectroscopic measurements confirmed the stoichiometry of the TiO₂nanoparticles. X-ray diffraction patterns showed a polycrystalline anatase structure of TiO₂. Transmission electron microscopy revealed that these particles are of nanoscale dimensions. Exact particle size and size distribution analyses were carried out by dynamic light scattering. The average particle size was determined to be 22 nm. The nanosize particles provided large surface area for photocatalysis and a large number of free surface-charge carriers, which are crucial for the enhancement of photocatalytic activity. To improve the photocatalytic activity, metal ions, including transition metal ions $(Pd^{2+},\;Pt^{4+},\;Fe^{3+})$ and lanthanide ion $(Nd^{3+})$ were added to pure TiO₂nanoparticles. The effects of dopants on photocatalytic kinetics were investigated by the degradation of 2-chlorophenol under an ultraviolet light source. The results showed that the TiO₂nanoparticles with the metal ion dopants have higher photocatalytic activity than undoped TiO₂. The $Nd^{3+}$ ion of these dopant metal ions showed the highest catalytic activity. The difference in the photocatalytic activity with different dopants is related to the different ionic radii of the dopants.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.973-976
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• 2008

염해환경에 건설되는 콘크리트 구조물은 염소이온과 같은 유해이온이 외부로부터 콘크리트 중으로 침투, 확산하여 철근을 부식시킴으로써 구조물의 내구성을 크게 저하시키기 때문에 구조물의 내구성 평가 또는 설계시 중요한 고려사항이 된다. 현재 설계에서 사용되는 확산계수는 각 나라마다 제시된 근거가 다르므로, 직접 비교 검토하는 것이 곤란할 뿐만 아니라 동일 환경조건에서 동일 배합의 콘크리트에 대한 수명예측 결과가 크게 상이한 결과를 초래하게 된다. 본 연구는 염해환경하 콘크리트의 염해 내구성능 품질관리를 적용하기 위해서 현장에서 적용할 수 외국에서 규격화된 시험방법들을 비교 검토하고 이들 시험 방법 가운데 품질관리에 적합한 시험방법을 선정하여 재령별 확산 특성과 침지 시험 결과를 비교하여 분석하였다. 슬래그 미분말을 혼입한 콘크리트의 경우 침지시험(NT Build 443)과 촉진시험(NT Build 492)의 상관성을 분석한다면, 이들 토대로 콘크리트 배합의 품질관리 기준 확산계수를 도출할 수 있을 것으로 판단된다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.885-888
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• 2008

본 연구에서는 도로공사의 교량 구조물 적용에 적합한 고성능 콘크리트의 배합도출을 목적으로 고로슬래그미분말을 사용한 고성능 콘크리트의 기초 물성을 평가하였다. 굳지않은 콘크리트의 유동성은 W/B에 상관없이 고로슬래그 미분말의 혼입율이 증가할수록 목표 슬럼프플로우를 만족시키기 위한 고성능감수제의 첨가율은 저하되는 것으로 나타났으며, 목표 공기량을 만족시키기 위한 AE제의 첨가량의 변화는 Plain 콘크리트와 동등한 것으로 나타났다. 고로슬래그 미분말의 경우 높은 혼입율로 인하여 수화반응이 지연되기 때문에 최고온도가 약 10시간 후에 도달되는 것으로 나타났으며, 혼입율 증가에 따른 단열 상승온도는 혼입율 45%까지 수화열 저감효과가 작게 나타났으나 혼입율 60%에서는 수화열이 약 $8^{\circ}C$가량 저감되었다. 이는 수화열이 작은 고로슬래그 미분말의 분량이 많아진 것에 기인된 것으로 사료된다. 압축강도 특성은 W/B=26, 30%에서는 고로슬래그 미분말 혼입율이 증가할수록 압축강도가 Plain 콘크리트에 비하여 약 10MPa가량 크게 증가되는 것으로 나타났지만, W/B=34%에서는 압축강도가 Plain 콘크리트와 거의 같은 수준으로 나타남으로써, 낮은 물결합재비에서 고로슬래그 미분말을 사용할 경우 압축강도 증진에 효과가 있는 것으로 사료된다.

• BMB Reports
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• 제40권1호
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• pp.95-99
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• 2007

Bovine coding region single nucleotide polymorphisms located proximal to quantitative trait loci were identified to facilitate bovine QTL fine mapping research. A total of 692,763 bovine SNPs was extracted from 39,432 UniGene clusters, and 53,446 candidate SNPs were found to be a depth >3. In order to validate the in silico SNPs experimentally, 186 animals representing 14 breeds and 100 mixed breeds were analyzed. Genotyping of 40 randomly selected candidate SNPs revealed that 43% of these SNPs ranged in frequency from 0.009 to 0.498. To identify non-synonymous SNPs and to correct for possible frameshift errors in the ESTs at the predicted SNP positions, we designed a program that determines coding regions by protein-sequence referencing, and identified 17,735 nsSNPs. The SNPs and bovine quantitative traits loci informations were integrated into a bovine SNP data: BcSNPdb (http://snugenome.snu.ac.kr/BtcSNP/). Currently there are 43 different kinds of quantitative traits available. Thus, these SNPs would serve as valuable resources for exploiting genomic variation that influence economically and agriculturally important traits in cows.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2002년도 추계학술대회 논문집
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• pp.100-107
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• 2002

This study investigates the seasonal variation and spatial distribution characteristics of pollutant load, as executing the quantity valuation of pollutant load inflowing into Yeong-Il bay from on-land including the Hyeong-san river. Annual total pollutant generating rate from Yeong-Il bay region are 202ton to BOD, 620ton to SS, 42ton to T-N, 16ton to T-P respectively, if expressly point out, pollutant generating rate from the Hyeong-san river is the greatest, which BOD ratio is 78.2%, SS 88.5%, T-N 62.5%, T-P 73.1%. As calculating Tank model with input value of daily precipitation and evaporation of 2001 year in drainage basin of the Hyeong-san river, Estimated result of the annual total river discharge effluencing from this river is $830{\times}106m^3$. As result to estimating annual total effluence rate outflowing at the rivers from each drainage basins, annual total inflow pollutant rate are BOD 10,633ton, SS 19,302ton, T-N 15,369ton, T-P 305ton. The III basin which is population congestion region of the Pohang-city drain away a good many pollutant load than the V basin including the Neang-Chun with wide drainage area. Especially, a great many T-N than T-P inflow into Yeong-Il bay. The accumulation of pollutant load effluenced from on-land will happen on at the inner coast region of Yeong-Il bay, finally we would make a prediction that the water quality will take a bad turn.