• IMMUNE NETWORK
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• v.6 no.3
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• pp.117-122
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• 2006

Background: Caveolin, a family of integral membrane proteins are a principal component of caveolae membranes. In this study, we investigated the effect of p38 kinase on differentiation and on inflammatory responses in sodium nitroprusside (SNP)-treated chondrocytes. Methods: Rabbit articular chondrocytes were prepared from cartilage slices of 2-week-old New Zealand white rabbits by enzymatic digestion. SNP was used as a nitric oxide (NO) donor. In this experiments measuring SNP dose response, primary chondrocytes were treated with various concentrations of SNP for 24h. The time course of the SNP response was determined by incubating cells with 1mM SNP for the indicated time period $(0{\sim}24h)$. The cyclooxygenase-2 (COX-2) and type II collagen expression levels were determined by immunoblot analysis, and prostaglandin $E_2\;(PGE_2)$ assay was used to measure the COX-2 activity. The tyrosine phosphorylation of caveolin-1 was determined by immunoblot analysis and immunostaining. Results: SNP treatment stimulated tyrosine phosphorylation of caveolin-1 and activation of p38 kinase. SNP additionally caused dedifferentiation and inflammatory response. We showed previously that SNP treatment stimulated activation of p38 kinase and ERK-1/-2. Inhibition of p38 kinase with SB203580 reduced caveolin-1 tyrosine phosphorylation and COX-2 expression but enhanced dedifferentiation, whereas inhibition of ERK with PD98059 did not affect caveolin-1 tyrosine phosphorylation levels, suggesting that ERK at least is not related to dedifferentiation and COX-2 expression through caveolin-1 tyrosine phosphorylation. Conclusion: Our results indicate that SNP in articular chondrocytes stimulates dedifferentiation and inflammatory response via p38 kinase signaling in association with caveolin-1 phosphorylation.

• KIEAE Journal
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• v.15 no.5
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• pp.47-57
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• 2015

Purpose: The purpose of this study is to set the type of 'the New-hanok type Public Buildings' through a case study for the hanok public buildings completed after 2000 years, and to analyze planned properties of the type. This is significant Establishing legal status of 'the New-hanok type Public Buildings' and seeing review of application possibilities of the type for providing a systematic government support measures of 'the New-hanok type public buildings' when models developing future. Method: Method of research is the first to examine the current laws and established the definition and legal status of 'the New-hanok type Public Buildings'. Followed by Setting the type classification criteria as to classify the type of 'the New-hanok type public buildings' and research architectural overview of selected cases by Literature, Internet searches, etc. After systematizing of the types classification of analysis cases, Characteristics of the type of the building structure looks catch classify in spatial structure, function, beauty. Finally, review application possibilities of the type for systematic government support measures establish when models developing of 'the New-hanok type Public Buildings' through a comprehensive analysis. Result: Selected cases were categorized as 3 types according by structural standard based on the core concept of 'the New-hanok type Public Buildings' set in this study. This can be divided into 'Wooden Structure type' and 'Composite structure - Convergence type' and 'Composite structure - juxtaposed type', 'Wooden Structure type' was re-classified by divided into '(1)Traditional Korean Wooden Structure' and '(2)Laminated Wood Wooden Structure'.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.5
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• pp.528-534
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• 2003

The 2-D hydraulic experimental results for the submerged rubble-mound structure, we have been concerned with the slability/function characteristics of the structures by the effects of wave force, scour/deposition at the toe and the wave transmission ratio at the lee-side sea. So, to investigate the variation characteristics of the wave transmission ratio which depended on a geometrical structure of the submerged breakwater profiles, the critical conditions for the depth of submergence and crest width were obviously presented. In summary, the results lead us to the conclusion that the wave control capabilities of submerged breakwaters by the variation of the submergence depth is higher than about 4 times the degree at the efficiency than the that of crest width. The destruction of the covering block at the crest generated at the region which was located between the maximum and minimum damage curve, and it's maximum damage/failure station from the toe of the structure was $0.2\;L_s.$ As the wave transmission coefficient and the slope of the structure increase, the damage/failure ratio and the maximum scour depth at the toe was extended, respectively. When the maximum scour depth happened, the destruction of the covering block which was located at the toe generated at the front of the submerged rubble-mound breakwater. Finally, it was found from the results that the optimization of the structure may be obtained by the efficient decision of the submergence depth and crest width in the permissible range of the wave transmission ratio.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.169-177
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• 1992

An alkaline protease producing microorganism was isolated from soil and identified as Streptomyces griseus HC-1141. The optimum culture condition of Streptomyces griseus HC- 1141 for the production of alkaline protease was as follows; 0.5% casein, 0.05% ammonium chloride, 0.1% ferrous sulfate. 2.0% lactose, pH 8.0 and 84 hrs. The enzyme was purified about 53 folds by ammonium sulfate treatment, DEAE-cellulose ion exchange chromatography and gel filtratioo on Sephadex G-150. The homogeneity of the purified enzyme was verified by polyacrylamide gel electrophoresis. The molecular weight was estimated to be 31,000 on sodium dodecyl sulfate polyacrylamide gel electrophoresis. This enzyme consists of glycine and glutamic acid as major amino acids. The N-terminal and C-terminal residues of the alkaline protease were leucine and histidine respectively.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.03a
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• pp.579-584
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• 2009

Arrangement of the facilities for improving harbor functions depends on sea and land conditions such as the ship's arrival and departure conditions, waves and tide. And the plan and the size of the facilities depend much on harbor and marine environment condition such as cargo quantity, ship size, ship traffic and seawater circulation. Among these, waves have so much effect on a breakwater design that it is the most important to understand their characteristics and to apply them to breakwater design. Therefore, to analyze the effect of waves characteristics over a rubble mound breakwater, we have calculated wave pressure by using numerical analysis at each tide level and have analyzed the effect of wave pressure on structure stability by conducting the stability analysis with the wave pressure. As a result, it is found that during low and mean tide level time the biggest wave pressure is estimated near calm water level. But during high tide time, the biggest wave pressure is estimated in front of capping. And the stability analysis indicates also that a structure is most unstable when low tide time wave pressure is acting on. After reviewing the stability of a structure by applying vertical and horizon wave forces, it is concluded that safety factor is lower than ordinary time(max. about 15%), is also reviewed when designing a rubble mound breakwater.

• Bulletin of the Korean Chemical Society
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• v.24 no.1
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• pp.49-54
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• 2003

TiO₂nanoparticles were synthesized using the metallorganic chemical vapor deposition process. Particles with and without metal ion dopants were obtained. X-ray photoelectron and energy dispersive X-ray spectroscopic measurements confirmed the stoichiometry of the TiO₂nanoparticles. X-ray diffraction patterns showed a polycrystalline anatase structure of TiO₂. Transmission electron microscopy revealed that these particles are of nanoscale dimensions. Exact particle size and size distribution analyses were carried out by dynamic light scattering. The average particle size was determined to be 22 nm. The nanosize particles provided large surface area for photocatalysis and a large number of free surface-charge carriers, which are crucial for the enhancement of photocatalytic activity. To improve the photocatalytic activity, metal ions, including transition metal ions $(Pd^{2+},\;Pt^{4+},\;Fe^{3+})$ and lanthanide ion $(Nd^{3+})$ were added to pure TiO₂nanoparticles. The effects of dopants on photocatalytic kinetics were investigated by the degradation of 2-chlorophenol under an ultraviolet light source. The results showed that the TiO₂nanoparticles with the metal ion dopants have higher photocatalytic activity than undoped TiO₂. The $Nd^{3+}$ ion of these dopant metal ions showed the highest catalytic activity. The difference in the photocatalytic activity with different dopants is related to the different ionic radii of the dopants.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.973-976
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• 2008

Recently, it is increasingly reported that the deterioration of concrete structure under marine environments is due to diffusion and penetration of chloride ions. It is very important to estimate the diffusion coefficient of chloride ion in concrete. Estimation methods of chloride diffusivity by concentration difference is time-consuming. Therefore, chloride diffusivity of concrete is mainly conducted by electrically accelerated method, which is accelerating the movement of chloride ion by potential difference. However, there has not been any proper method for field quality control to closely determine the diffusion coefficient of chloride ion through accelerated tests using potential difference. In this paper, the various test methods for determination of chloride diffusion coefficient in concrete were investigated through comparison accelerated tests. From the results of estimated diffusion coefficient of chloride ion, relationship between the ponding test and acceleration test was examined.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.885-888
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• 2008

In recent years, the terminology "High-Performance Concrete(HPC)" has been introduced into the construction industry. Most high-performance concretes have a high cementitious content and a low water-cementitious material ratio. The proportions of the individual constituents vary depending on local preferences and local materials. Therefore, many trial batches are usually necessary before a successful mix is developed. The objective of this experiments is to investigate the fundamental properties of high performance concrete based binary cimentitious materials such as ordinary portland cement and ground granulated blast furnace slag. The results from the study will be utilized as the basic data and guideline in making standard mixproportions and the manufacture, construction work and quality control of HPC

• BMB Reports
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• v.40 no.1
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• pp.95-99
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• 2007

Bovine coding region single nucleotide polymorphisms located proximal to quantitative trait loci were identified to facilitate bovine QTL fine mapping research. A total of 692,763 bovine SNPs was extracted from 39,432 UniGene clusters, and 53,446 candidate SNPs were found to be a depth >3. In order to validate the in silico SNPs experimentally, 186 animals representing 14 breeds and 100 mixed breeds were analyzed. Genotyping of 40 randomly selected candidate SNPs revealed that 43% of these SNPs ranged in frequency from 0.009 to 0.498. To identify non-synonymous SNPs and to correct for possible frameshift errors in the ESTs at the predicted SNP positions, we designed a program that determines coding regions by protein-sequence referencing, and identified 17,735 nsSNPs. The SNPs and bovine quantitative traits loci informations were integrated into a bovine SNP data: BcSNPdb (http://snugenome.snu.ac.kr/BtcSNP/). Currently there are 43 different kinds of quantitative traits available. Thus, these SNPs would serve as valuable resources for exploiting genomic variation that influence economically and agriculturally important traits in cows.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2002.10a
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• pp.100-107
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• 2002

This study investigates the seasonal variation and spatial distribution characteristics of pollutant load, as executing the quantity valuation of pollutant load inflowing into Yeong-Il bay from on-land including the Hyeong-san river. Annual total pollutant generating rate from Yeong-Il bay region are 202ton to BOD, 620ton to SS, 42ton to T-N, 16ton to T-P respectively, if expressly point out, pollutant generating rate from the Hyeong-san river is the greatest, which BOD ratio is 78.2%, SS 88.5%, T-N 62.5%, T-P 73.1%. As calculating Tank model with input value of daily precipitation and evaporation of 2001 year in drainage basin of the Hyeong-san river, Estimated result of the annual total river discharge effluencing from this river is $830{\times}106m^3$. As result to estimating annual total effluence rate outflowing at the rivers from each drainage basins, annual total inflow pollutant rate are BOD 10,633ton, SS 19,302ton, T-N 15,369ton, T-P 305ton. The III basin which is population congestion region of the Pohang-city drain away a good many pollutant load than the V basin including the Neang-Chun with wide drainage area. Especially, a great many T-N than T-P inflow into Yeong-Il bay. The accumulation of pollutant load effluenced from on-land will happen on at the inner coast region of Yeong-Il bay, finally we would make a prediction that the water quality will take a bad turn.