• 멤브레인
• /
• 제26권1호
• /
• pp.26-31
• /
• 2016

폴리카프로락톤(PCL)에 NaCl을 혼합한 용액을 블레이드법에 의하여 막형태로 제조한 후 NaCl을 추출하는 염출법을 이용하여 조직공학적으로 사용할 3차원 다공망을 갖는 멤브레인 형태의 지지체를 제조하였다. 본 연구에서는 성형된 멤브레인의 건조조건과, NaCl 입자의 크기, NaCl의 혼합량을 각각 다르게 하여 제조하였다. 별도로 제작한 고분자용액 공급장치를 이용하여 PCL/클로로포름($CHCl_3$) 용액에 NaCl 입자가 균일하게 혼합된 용액을 유리판에 분주하여 필름 어플리케이터를 사용하여 블레이드법에 의한 멤브레인을 제조하였다. 멤브레인 지지체에는 NaCl 입자에 의한 거대기공과 거대기공을 이루는 구조벽에서는 $CHCl_3$의 증발에 의한 미세기공이 함께 복합적으로 상호 연결되어 형성되었다.

• Macromolecular Research
• /
• 제9권5호
• /
• pp.267-276
• /
• 2001

In order to endow with new bioactive functionality from demineralized bone particle (DBP) as natural source to poly(L-lactide) (PLA) synthetic biodegradable polymer, porous DBP/PLA as natural/synthetic composite scaffolds were prepared and compared by means of the emulsion freeze drying and solvent casting/salt leaching methods for the possibility of the application of tissue engineered bone and cartilage. For the emulsion freeze drying method, it was observed that the pore size decreased in the order of 79$\mu\textrm{m}$ (PLA control) > 47$\mu\textrm{m}$ (20% of DBP) > 23 $\mu\textrm{m}$ (40% of DBP) > 15$\mu\textrm{m}$ (80% of DBP). Porosities as well as specific pore areas decreased with increasing the amount of DBR. It can be explained that DBP acts like emulsifier resulting in stabilizing water droplet in emulsion. For the solvent casting/salt leaching method, a uniform distribution of well interconnected pores from the surface to core region were observed the pore size of 80 ∼70 $\mu\textrm{m}$ independent with DBP amount. Porosities as well as specific pore areas also were almost same. For pore size distribution by the mercury intrusion porosimeter analysis between the two methods, the pore size distribution of the emulsion freeze drying method was broader than that of the solvent casting/salt leaching method due to the mechanism of emulsion formation. Scaffolds of PLA alone, DBP/PLA of 40 and 80%, and DBP powder were implanted on the back of athymic nude mouse to observe the effect of DBP on the induction of cells proliferation by hematoxylin and eosin staining for 8 weeks. It was observed that the effect of DBP/PLA scaffolds on bone induction are stronger than PLA scaffolds, even though the bone induction effect of DBP/PLA scaffold might be lowered than only DBP powder, that is to say, in the order of DBP only > DBP/PLA scaffolds of 40 and 80% DBP > PLA scaffolds only for osteoinduction activity. In conclusion, it seems that DBP plays an important role for bone induction in DBP/PLA scaffolds for the application of tissue engineering area.

• 한국약용작물학회:학술대회논문집
• /
• 한국약용작물학회 2008년도 춘계학술발표회
• /
• pp.97-98
• /
• 2008

• 한국농공학회지
• /
• 제30권4호
• /
• pp.127-133
• /
• 1988

This study was performed to obtain the basic data analyzing salt movement and desalinization effects, and two different desalinization experiments through leaching and rinsing were carried out, using samples of silt loam soil and silty clay loam soil collected in reclaimed tidelands. The relationships between the electrical conductivity of saturation extract and the electrical conductivity at various dilutions, and the correlations between electrical conductivity, total salt concentration, exchangeable sodium percentage and pH during the desalinization of reclaimed tidelands, were analyzed by the statistical method. The results obtained from this study were summarized as follows: 1.The sample soils used in this study were saline-sodic soils in accordance with the USDA classi- fication system of salt affected soils. 2.The electrical conductivity of saturation extract could be estimated conveniently, using the electrical conductivity of extract from various different soil-water suspensions. 3.The total salt concentration could be expressed in the electrical conductivity, but there was a little difference by soil textures. 4.The regression analysis showed that the relationship between the electrical conductivity of saturation extract and the exchangeable sodium percentage during the desalinization of reclaimed lands could be described by a linear regression equation. 5.The value of pH showed a tendency to increase according as the exchangeable sodium percentage decreased during the desalinization of reclaimed tidelands.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
• /
• pp.421-424
• /
• 2000

Cartilage injuries are frequent nowadays. The previous surgical treatment of cartilage defect was limited. Another approach in the treatment of cartilage injuries is the use of reconstitute cartilage consisting of chondrocytes cultured in suitable biodegradable scaffolds. Current studies have demonstrated the compatibility of chondrocytes with different biomaterials and the chondrogenesis in various types of porous scaffolds. The cell ingrowth into the porous scaffolds is modulated by initial cell loading efficiency. Therefore, well-interconnected pore structure and even pore distribution of the scaffolds are essential for efficient cell seeding. According to our previous work, well-interconnected macroporous scaffolds can be prepared by gas-foaming/salt-leaching method using ammonium bicarbonate salt as porogen additives. In this work, primary chondrocytes were cultured in PLGA 65/35 scaffolds fabricated by using our method. Cells seeded in the scaffolds showed well distribution by agitated seeding method. Histochemical staining of proteoglycans present in the scaffolds was used to visualize the chondrocyte ingrowth in the scaffolds. At 3 weeks, the population of chondrocytes was increased for the most part of the scaffolds, and extra cellular matrix (ECM) secretion was increased as culture periods progressed.

• 폴리머
• /
• 제29권2호
• /
• pp.198-203
• /
• 2005

용융 압출 발포에 의하여 폴리락틱산(PLLA) 지지체를 제조하고 발포 조건이 지지체의 구조와 기계적 특성에 미치는 영향을 살펴보고 이를 염 추출법에 의하여 제조된 지지체와 비교하였다. 발포제를 함유한 PLLA 용융체가 압출기 및 다이에서 체류하는 시간이 발포제의 무게를 최대로 감소시키는 시간과 일치하여야 최적의 PLLA 지지체의 구조를 얻을 수 있음을 확인하였다. PLLA지지체의 구조를 유지하기 위해서는 발포제의 함량을 $10\;wt\%$ 이하로 조절해야 하며 PLLA 지지체의 다공도는 PLLA의 압출기에서의 체류시간에 가장 큰 영향을 받음을 알 수 있었다. 용융 압출 발포에 의하여 얻어진 지지체는 일반적으로 염 추출법에 의하여 제조된 지지체보다 다소 낮은 다공도를 갖지만 발포 조건의 조절에 의하여 적절한 다공 크기와 다공의 연결성을 가지며 동시에 우수한 기계적 특성을 가져 경조직 재생(hard tissue regeneration)용으로 사용이 가능할 것으로 사료된다.

• 한국농공학회지
• /
• 제20권2호
• /
• pp.4695-4707
• /
• 1978

The objeative of this study is to study how to rapidly convert tidal land into cultivable land. The study of a rapid, reasonable desalinization method is conducted at Namyang tidal land which represents soil texture of tidal lands along the south west costa larea of Korea. Therefore, Researches were made at many Pilots in order to find a way of high efficiency of leaching with simpler facilities and cheaper costs. The results of study are briefly summarized as follaws: 1. Subdrainage efficieny is 35%. This is a Poorly drained area, and needs longer leaching desalinization period. 2. The efficieny of desalinization in P.V.C 16 meters plot is the same as that of mole drainage 2 meters plot. P.V.C 4 meters plot has desalinization effect as much as two times compared to P.V.C 16 meters plot. 3. Because the soil texture is silty-clay, desalinization in non-treated plot of sub-drainage and surface drainage desalinization take three times longer period in comparision with P.V.C 4 meters plot. 4. As to the desalinization rate of soluble salt in the soil, the efficieny of desalinization of the topsoil in P.V.C plots was 50% higher than that of mole drainage plot and about 170% higher than that of non-treated plot. In the deep soil salt accumulation at topsoil was observed in non-treated and mole drainage plots, but efficiency in P.V.C polt is about 40 times as high as that of mole drainage and non-treated plot. 5. As to the results of use gypsum and lime as sub-drainage soil improver, gypsum was 60% more efficieny than lime in the continuously inundated plot and 44% in the intermittently inundated plot. The efficieny of gypsum and lime in the intermittently flooded plot is 35% and 42% higher than that of continuously flooded plot reapeaticee1y.

• 한국임상수의학회지
• /
• 제33권6호
• /
• pp.332-339
• /
• 2016

Polycaprolactone (PCL) scaffold have been developed as an alternative to natural donor tissue to repair a large osteochondral defect. The objective of this study is to evaluate efficacy and biocompatibility of bilayer PCL scaffold implanted for osteochondral repair in rabbit. Twenty-two male New Zealand White rabbits were used in this animal experiment. Rabbits were divided into three groups. Experimental surgery was carried out under general anesthesia. Osteochondral defects (5 mm diameter and 5 mm deep) were made in the center of the patellar groove using a 5 mm diameter biopsy punch. In group I (3D plotting) and group II (salt-leaching), the scaffold was implanted using the press-fitted technique into the defect. In control group, after osteochondral defect was created, the defect was left without implant. After four and eight weeks, rabbits were sacrificed and the defects were evaluated by macro -and microscopical methods. There were not found animal death and severe inflammatory evidence during the experimental periods. There were no significant differences between the experimental groups in gross evaluation. However the group I scored significantly higher than group II at 8 weeks in histological evaluation (P < 0.05). The 3-D plotting PCL scaffold was more suitable method for reconstruction of osteochondral defect than a salt-leaching PCL scaffold.

• 공업화학
• /
• 제9권3호
• /
• pp.407-412
• /
• 1998

본 연구는 불탄산염 희토광류(bastnasite)의 황산화반응과 수침물을 수행함으로써, 불탄산염 희토류광의 최적 침출조건을 고찰하고자 하였으며, 또한 수침출용액으로부터 산도조절법에 의하여 세륨을 기타 희토류원소로부터 분리하고자 하였다. Bastnasite 정광의 황산화 반응과 수침출의 최적 조건은 bastnasite 정광대비 황산의 첨가량은 당량비 2.5이었으며, 소성온도는 $600^{\circ}C$, 소성시간은 2시간이었으며, 수침출시 광액농도는 9.1%이었다. 이러한 조건에서 희토류산화물의 침출률은 약 93%이었다. Bastnasite 정광 황산화분해 반응산물의 수침출 결과 얻은 용액으로부터 산도조절법에 의한 세륨 회수시, 산화제로 과산화수소수 2당량을 사용하여 침출용액의 pH 5에서 산화 그리고 다시 용액의 pH를 2로 낮추어 세륨을 세륨복염 및 세륨수산화물로 침전 회수하고, 다시 과산화수소수 1당량으로 산화침전을 반복하는 다단 산화 침전을 수행한 결과 회수율 60%, 세륨 품위 80% 이상의 세륨수산화물을 제조할 수 있었다.

• 한국농공학회지
• /
• 제31권2호
• /
• pp.92-103
• /
• 1989

This study was performed in order to produce the basic data for devising irrigation project and desalinization countermeasure through analyzing salt movement and desalinzation effects. The Desalinization experiments with water management practices were carried out, using the soil samples collected in New Manguem tideland. The changes of electrical conductivity, exchangeable sodium percentage, pH and hydraulic conductivity during the desalinization experiments, and the correlations between various factors influencing desalinization, were analyzed by the statistical method. The results obtained from this study were summarized as follows: 1. The sample soils used in this study were salin-sotlic soils with the high electrical conductivity and the high exchangeable sodium percentage, and the soil texture was silt loam. 2. A large amount of the soluble salts was removed in the begining of desalinization experiments. The initial electrical conductivity and the initial exchangeable sodium percentage decreased considerably in the beginning, and were gradually slow in the rate of decrease 3. The value of pH showed a tendency to increase during the desalinization and were little by little slow in the rate of increase, and could be estimated by the regression equation. 4. The initial hydraulic conductivity were raised greatly with gypsum treatment and the penmeability was maintained adequately, The hydraulic conductivity and the leaching time elapsed during the desalinization could be estimated by the regression equation. 5. The water requirement for desalinization with various water management practices could be estimated for a given electrical conductivity, exchangeable sodium percentage, and pH reading respectively.