• Title/Summary/Keyword: Safety standard

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Comparison of Culture, Conventional and Real-time PCR Methods for Listeria monocytogenes in Foods

  • Kim, Dong-Hyeon;Chon, Jung-Whan;Kim, Hyunsook;Kim, Hong-Seok;Choi, Dasom;Kim, Young-Ji;Yim, Jin-Hyeok;Moon, Jin-San;Seo, Kun-Ho
    • Food Science of Animal Resources
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    • v.34 no.5
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    • pp.665-673
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    • 2014
  • We compared standard culture methods as well as conventional PCR and real-time PCR for the detection of Listeria monocytogenes (L. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. No statistical differences were observed in sensitivity between the two selective media in all foods. In total, real-time PCR assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compared with standard culture methods. Conventional culture methods showed poor performance in detecting L. monocytogenes in food with high levels of background microflora, generating numerous false negative results. While the detection of L. monocytogenes in fresh cut vegetable by culture methods was hindered only by L. innocua, various background microflora, such as L. innocua, L. welshimeri, L. grayi, and Enterococcus faecalis appeared on the two selective media as presumptive positive colonies in raw beef indicating the necessity of improvement of current selective media. It appears that real-time PCR is an effective and sensitive presumptive screening tool for L. monocytogenes in various types of foods, especially foods samples with high levels of background microflora, thus complementing standard culture methodologies.

A Study on the Hazardousness Evaluation and the Inhalation Toxicity of Methylcyclohexane (메틸사이클로핵산 (methylcyclohexane)의 흡입독성과 유해성 평가)

  • Kim Hyeon-Yeong;Lee Sung-Bae;Kang Min-Gu;Song Si-Hwan
    • Environmental Analysis Health and Toxicology
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    • v.21 no.2 s.53
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    • pp.173-184
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    • 2006
  • From the harmfulness expectation test conducted through a toxicity anticipation program, methylcyclohexane turned out to be harmful and simulative, but no carcinogenicity was anticipated. In a four-hour acute inhalation toxicity test, the result showed that lethal concentration ($LC_{50}$) was 3,750 ppm (15,054 mg/L), which was identified as a harmful substance on the basis of the harmful substance classification standard $2 of the Industrial safety and health law. methylcyclohexane fell under the category $4(2,500 substance from the GHS standard acute toxicity harmfulness classification. Also, from subchronic inhalation toxicity test that included 6 hours a day, five days a week, and for 13 weeks, we could observe weight, activity, long term weight, blood and blood biochemical influence from the exposure of test substance. No-observed effect level (NOEL) was determined below $100{\sim}400ppm$ inboth male and female. This material falls under the Category 2 ($50{\sim}250ppm/6hours/90days$) in the GHS (Globally Harmonized System) standard trace long-term whole body toxicity repeated exposure, and can be classified as a harmful substance in accordance with the Industrial Safety and Health Law harmful substance standard $NOEL{\leq}0.5mg/L/6hr/90day$ (rat).

A Study on the Necessity of Korean Standard and Code of Safety for Diving System (잠수 시스템의 국내 표준 및 안전 기준 제정 필요성에 관한 연구)

  • Park, Jong-Yeob;Kang, Sin-Young
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.6
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    • pp.856-860
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    • 2011
  • The standard and code of safety for diving systems include requirements in the area of diving plant, personnel qualification, diving medicine and operation. In this study the necessity of Korean standard and code of safety for diving system is suggested through the review and comparison of the diving system regulations of domestic as well as foreign governments and other private organizations. Furthermore, by reviewing the status of oversea's diving organizations the proposition was made which can lead the commercial diving sector to international cooperation.

Construction of a knowledge-base for safety standards to support the design of household electrical appliances (가전제품의 설계지원을 위한 안전규격 지식베이스의 구축)

  • Lee, Hyo-Seop;Han, Soon-Hung
    • Journal of the Korean Society for Precision Engineering
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    • v.11 no.4
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    • pp.106-113
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    • 1994
  • Household electrical appliances should be designed to satisfy safety standards. An expert system is implemented to support the design process. The general-purpose expert system shell. ART-IM which is running under MS-DOS environment, is used to construct the knowledge-base. A set of rules has been extracted from the EN 60 335-1 that is British standard specification for the safety of household and similar electrical appliances. The main focus of this paper is on codes that have systematic and mormative structures. The internal structure of the safety standard is analysed to improve the process of rule extraction.

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Technical Specifications for Manufacturer Approval in Railway Safety Law (철도안전법의 제작자 승인 기술기준에 대한 연구)

  • Lee, Hwan-Deok;Jung, Won
    • Journal of Applied Reliability
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    • v.15 no.1
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    • pp.19-26
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    • 2015
  • The amended law of railway safety in Korea has recently come into effect in order to strengthen the railway safety management system. The new law, which took effect March 1, 2014, will implement stricter oversight of railway companies. As a result, a company that manufactures railroad system or components for domestic use must obtain an approval in accordance with the technical specifications of manufacturer approval. Although Korea had established the legal system in enforcing railway safety, the government wants the companies continue to develop the more improved safety systems until they gain competitive edge on the world class railway manufacturers. This paper presents an in-depth analysis of the technical specifications for manufacturer approval in International Railway Industry Standard (IRIS), which is the global standard. This paper also proposes measures and guidelines that would help Korean manufacturers those who want further develop their safety management systems, as a prerequisite for them to obtain the manufacturer approval.

A Study on Electromagnetic Compatibility and Safety Standards for Motorcycles (이륜자동차의 전자파 적합성과 안전기준에 관한 연구)

  • Woo, Hyungu;Yong, Boojoong
    • Journal of Auto-vehicle Safety Association
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    • v.11 no.1
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    • pp.17-22
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    • 2019
  • Nowadays many two-wheeled motorcycles are used in leisure and express delivery industries. But studies of the electromagentic compatibility on motorcycles have seldom been performed and published. This paper has studied the electromagnetic safety of motorcycles considering international and domestic regulations and standards. Also, a series of experiments have been performed to evaluate the level of electromagnetic compatibility of commercially available motorcycles. As a result, detailed experimental procedures are proposed to secure the electromagnetic safety and can be used to confirm or revise the Korea Motor Vehicle Safety Standards.

A Study on the Evaluation of the Pipe Fracture Characteristic (실배관 파괴특성 평가에 관한 연구)

  • Park Jae-Sil;Kim Young-Jin;Seok Chang-Sung
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.29 no.1 s.232
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    • pp.107-114
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    • 2005
  • In order to analyze the elastic-plastic fracture behavior of a structure, the fracture resistance curve of the material should be known first. The standard CT specimen was used to obtain the fracture resistance curves of a piping system. However, it is known that the fracture resistance curve by the standard CT specimen is very conservative to evaluate the integrity of a structure. Also the fracture resistance curve is effected by the specimen geometry and the dimensions because of the constraint effect. The objective of this paper is to be certain the conservativeness of the fracture resistance curve by the standard CT specimen and to provide an additional safety margin. For these, the fracture tests using a real pipe specimen and the standard CT specimen test were performed. A 4-point bending jig was manufactured for the pipe test and the direct current potential drop method was used to measure the crack extension and the length for the pipe test. Also finite element analyses were performed with a CT specimen and a pipe in order to prove the additional safety margin. From the result of tests and analyses of the pipe and the standard CT specimen, it was observed that the fracture analysis with the standard CT specimen is conservative and the additional safety margin was proved.

Reviewing the Operation of the Navy Safety Management System and Its Direction (해군 안전관리체계 운영에 대한 검토 및 발전 방향 고찰)

  • Jeongwoo Han;Kijae Kim;Wonyoung Lee;Kyoshik Park
    • Journal of the Society of Disaster Information
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    • v.19 no.4
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    • pp.859-868
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    • 2023
  • Purpose: In order to enhance safety, the Korea Navy is making efforts to introduce a safety and health management system and to develop a risk assessment system, while safety accidents keeps occurring. A development plan was proposed through an inspection of the Safety Management System(SMS). Method: To diagnose the Korea Navy's safety management system, changes in the safety environment, documentation system, and safety and health management system operation were reviewed, points to be improved were found, and improvement directions were derived. Result: There is a need for an SMS standard that can present the Korea Navy's safety policy and safety management direction, and a safety program that provides guidelines for implementing it in a standardized manner. To this end, we were able to confirm the feasibility of linking the requirements of the US Navy's SMS and the international standard ISO 45001 standard together with the Korea Navy's safety management system. Conclusion: The Korea Navy's safety management system needs to clearly present safety policies and standards, and have public confidence and effectiveness in safety work through a standard program that can implement them, and for this, the development of a Navy SMS is necessary.

A study on NCS based qualification plan development procedure of industrial safety field - Focused on the industrial safety, industrial health, nondestructive section- (산업안전 분야 NCS기반 자격설계 개발과정에 관한 연구 - 산업안전, 산업보건, 비파괴 분야를 중심으로 -)

  • Yi, Shin-jae;Yang, wook;Yoon, Young-Ju;Choi, Seo-Yeon
    • Journal of the Korea Safety Management & Science
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    • v.17 no.3
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    • pp.9-14
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    • 2015
  • The study is conducted in order to establish validity of industrial safety field's neo-qualification composition by suggesting reengineering procedure of NCS based industrial safety field's qualifying occupational plan. Industrial safety fields are first classified into great classification which are environment energy safety, and then into sub-classification which are industrial safety management, industrial health management, and nondestructive test. NCS based neo-qualification plan is deveopled into progress as following steps; First, planning NCS neo-qualification system. Second, presenting work education combined program certification standard. Third, developing NCS based neo-qualification assessment standard. Fourth, developing work education qualification prototype problem. The process of qualification plan was composed with Worksheet 14 and with 4phase being completing summary of each section, certification and development of assessment standard, and development of prototype problem. As a result, Industrial safety manager was redesigned into Industrial sfety manager and Construction Safety Manager, and Occupational Health Care Manager was redesigned into Occupational Health Care Manager and Industrial Health Manager. This study, in conclusion, suggests development of qualifying occupation that could be applied to the integrated management system and development of future industrial safety field's specialized neo-qualifying occupation.