• Title/Summary/Keyword: SW06

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Interactions between Indole-3-acetic Acid Producing Acinetobacter sp. SW5 and Growth of Tomato Plant (Indole-3-acetic acid를 생성하는 Acinetobacter sp. SW5와 토마토 식물 간의 상호작용)

  • Kwon, Hyeok-Do;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.302-307
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    • 2014
  • Many rhizobacteria can promote plant growth through various direct or indirect mechanisms, and their production of phytohormones such as indole-3-acetic acid (IAA) may have pronounced effects on growth and development of plants. Rhizobacterial strain isolated from rhizosphere of foxtail (Setaria viridis), Acinetobacter sp. SW5 produced 118.1 mg/L of IAA and 4.5 mg/L of gibberellin ($GA_3$) in brain heart broth medium at 2 and 1 day of incubation, respectively. In a pot test the lengths of stem and root and fresh weight of the germinated tomato seedlings treated with Acinetobacter sp. SW5 significantly increased by 26.3, 33.3, and 105.3%, respectively compared to those of the uninoculated control in 12 weeks of cultivation. When the root exudate secreted from tomato seedlings was analyzed by HPLC, 3.75 ng mg tomato $root^{-1}$ of tryptophan which is an IAA precursor was detected. Acinetobacter sp. SW5 could produce $4.06{\mu}M$ of IAA from root exudate from 8 tomato seedlings. Together with the capability of growth of Acinetobacter sp. SW5 in the tomato root exudates, this IAA secreted by bacteria might contribute to enhance the growth of tomato plants.

Time-domain Geoacoustic Inversion of Short-range Acoustic Data with Fluctuating Arrivals (시변동이 있는 근거리 음향신호의 시간영역 지음향학적 역산)

  • Park, Cheolsoo;Seong, Woojae;Gerstoft, Peter;Hodgkiss, William S.
    • The Journal of the Acoustical Society of Korea
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    • v.32 no.4
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    • pp.308-316
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    • 2013
  • A set of experiments (Shallow Water 2006, SW06) was carried out in shallow water near the New Jersey shelf break in summer 2006. Significant fluctuations in direct and surface reflected arrivals were observed from the chirp data (1100~2900 Hz) measured on a vertical line array. This paper presents a geoacoustic inverssion technique for short-range acoustic data with fluctuating arrivals and inversion results of experimental data. In order to reduce effects of random sea surface on the inversion, the acoustic energy back-propagated from the array to the source through direct and bottom-reflected paths is defined as the objective function. A multi-step inversion scheme is applied to the data using VFSR (Very Fast Simulated Reannealing) optimization technique. The inversion results show a source depth oscillation period equal to the measured ocean surface wave period. The inverted bottom sound speed is 1645 m/s and is similar to that estimated by other work at the same site.

Research on the Teaching Building-blocks in Elementary Geometry Class using 3D Visualization SW (3D Visualization SW를 활용한 초등학교 쌓기나무 도형교육에 관한 연구)

  • Bae, Hun Joong;Kim, Jong-seong
    • The Journal of the Korea Contents Association
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    • v.17 no.6
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    • pp.71-80
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    • 2017
  • The standards for achievement levels for building blocks in elementary geometry class is to enhance spatial cognitive ability through practices describing shape patterns of building blocks observed from different directions. However, most of building block in the textbook is described from only one perspective. Even worse, some examples in the textbook are almost impossible to observe in the real world. Contrary to this, simulated views by Wings3D has shown that each box may look quite differently from different angles let alone the size of each box. Using Wings3D, it is also very easy to build different types of building blocks with various levels of difficulty in the virtual space. Based on these results, in this study, 3D visualization SW is suggested as a potential pedagogical tool for the elementary geometry class to help kids perceive objects in space more precisely. We have shown that 3D visualization SW such as Wings3D could be a powerful, compact 3D SW for most of subjects which are covered in elementary geometry education. Wings3D has another advantage of economic open source SW fully compatible with school PCs.

벤젠 이합체와 그 치환체의 양자역학을 통한 π-π interaction의 계산

  • Jo, Ji-Seong;Cho, Art.
    • Proceeding of EDISON Challenge
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    • 2014.03a
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    • pp.385-397
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    • 2014
  • 신약을 개발하거나 단백질 구조를 예측하는데 Molecular Mechanics (MM)의 방법을 사용한다. 하지만, MM 만으로는 자연현상에서 일어나는 결과를 정확하게 기술하기 어렵다. 본 연구는 기존의 MM 방법으로는 정확히 예측이 불가능한 비 공유결합 중 하나인 ${\pi}-{\pi}$ interaction을 양자역학 계산을 통해 정확한 예측이 가능한지 보았다. ${\pi}-{\pi}$ interaction 이란 생체 내, 의약 화합물에서 발견되는 결합이기 때문에, 단백질과 결합하는 구조의 예측이 중요하다고 할 수 있다. 본 실험은 ${\pi}-{\pi}$ interaction을 갖는 Sandwich, T shape, 그리고 Parallel displaced 세 가지 모형을 가지고 양자역학 계산을 수행하였다. 양자역학 계산은 DFT의 세가지 함수 M06_2X, M05_2X, B3LYP를 이용하였다. 실험결과에서 세 가지 함수가 각기 다른 결과를 보였는데, M06_2X의 결과에서 ${\pi}-{\pi}$ interaction을 더 정확하게 계산하였다. 이러한 결과를 바탕으로, 양자역학의 방법을 통해 MM에서는 예측이 불가능한 ${\pi}-{\pi}$ interaction을 계산 할 수 있고 이 부분을 고려하여 화합물 간의 결합구조를 예측을 향상시킬 수 있다.

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수용액중에서 모노에탄올아민의 CO2 흡수반응의 반응자유에너지의 밀도범함수 계산모델.

  • Jang, Yong-Hun;Kim, Yong-Jun;Wi, E-Hwan;Lee, Sang-Yeon
    • Proceeding of EDISON Challenge
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    • 2014.03a
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    • pp.53-61
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    • 2014
  • 온실가스 증가로 인한 지구온난화 문제가 범세계적인 문제로 대두되고 있는 가운데, 특히 온실가스 중 약 76%이상을 차지하는 이산화탄소를 흡수하기 위한 흡수제 개발에 여러 국가들이 심혈을 기울이고 있다. 그 중 이산화탄소 흡수제로 가장 상용화 되어 있는 모노에탄올아민(monoethanolamine, MEA)은 분자량이 작아 몰 농도비에 따른 이산화탄소의 흡수에 유리하고 반응속도가 빠르다는 장점이 있으나, 재생에 필요한 에너지가 높다는 단점이 존재한다. 수용액중에서 MEA가 $CO_2$를 흡수하는 반응의 반응자유에너지는 반응메카니즘을 이해하는데 가장 기본적인 도구이다. 본 연구에서는 B3LYP, M06-2X의 밀도범함수를 이용하여 MEA의 $CO_2$ 흡수반응의 반응자유에너지를 계산하는 계산모델을 선정하였다. 수용액에서 MEA가 $CO_2$를 흡수하는 반응의 반응자유에너지를 밀도범함수를 이용하여 계산할 때는, 수용액상태에서 화학종의 분자구조를 최적화하는 것이 필요하였다. 또한 M06-2X 밀도범함수가 B3LYP 밀도범함수보다 좋은 결과를 주었으며, 분산보정을 하는 것이 보다 좋은 결과를 주었다.

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Particle Swarm Optimization Using Adaptive Boundary Correction for Human Activity Recognition

  • Kwon, Yongjin;Heo, Seonguk;Kang, Kyuchang;Bae, Changseok
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.8 no.6
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    • pp.2070-2086
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    • 2014
  • As a kind of personal lifelog data, activity data have been considered as one of the most compelling information to understand the user's habits and to calibrate diagnoses. In this paper, we proposed a robust algorithm to sampling rates for human activity recognition, which identifies a user's activity using accelerations from a triaxial accelerometer in a smartphone. Although a high sampling rate is required for high accuracy, it is not desirable for actual smartphone usage, battery consumption, or storage occupancy. Activity recognitions with well-known algorithms, including MLP, C4.5, or SVM, suffer from a loss of accuracy when a sampling rate of accelerometers decreases. Thus, we start from particle swarm optimization (PSO), which has relatively better tolerance to declines in sampling rates, and we propose PSO with an adaptive boundary correction (ABC) approach. PSO with ABC is tolerant of various sampling rate in that it identifies all data by adjusting the classification boundaries of each activity. The experimental results show that PSO with ABC has better tolerance to changes of sampling rates of an accelerometer than PSO without ABC and other methods. In particular, PSO with ABC is 6%, 25%, and 35% better than PSO without ABC for sitting, standing, and walking, respectively, at a sampling period of 32 seconds. PSO with ABC is the only algorithm that guarantees at least 80% accuracy for every activity at a sampling period of smaller than or equal to 8 seconds.

Use Case Points Estimation for the Software Cost Appraisal (소프트웨어 개발비 감정을 위한 유스케이스 점수 추정)

  • Kwon, Ki-Tae
    • Journal of Software Assessment and Valuation
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    • v.16 no.1
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    • pp.27-36
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    • 2020
  • The software development cost appraisal is treated as a part of the program completion appraisal, and the software engineering methodology is applied. In particular, software cost estimation techniques have been actively applied. For more information about the software development costs calculation, we can refer to the "SW cost estimation guide". Although successful appraisal of a number of development costs based on the guide has been processed, but a number of cases requiring discussion of appraisal results have been discovered. In this study, we propose a use case-based size estimation method to maintain the accuracy and consistency of size estimation. As a result of performing performance evaluation of the proposed method in an environment similar to the development cost appraisal case, it was proved that the accuracy was improved over the existing function points method.

A Virtual Battlefield Situation Dataset Generation for Battlefield Analysis based on Artificial Intelligence

  • Cho, Eunji;Jin, Soyeon;Shin, Yukyung;Lee, Woosin
    • Journal of the Korea Society of Computer and Information
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    • v.27 no.6
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    • pp.33-42
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    • 2022
  • In the existing intelligent command control system study, the analysis results of the commander's battlefield situation questions are provided from knowledge-based situation data. Analysis reporters write these results in various expressions of natural language. However, it is important to analyze situations about information and intelligence according to context. Analyzing the battlefield situation using artificial intelligence is necessary. We propose a virtual dataset generation method based on battlefield simulation scenarios in order to provide a dataset necessary for the battlefield situation analysis based on artificial intelligence. Dataset is generated after identifying battlefield knowledge elements in scenarios. When a candidate hypothesis is created, a unit hypothesis is automatically created. By combining unit hypotheses, similar identification hypothesis combinations are generated. An aggregation hypothesis is generated by grouping candidate hypotheses. Dataset generator SW implementation demonstrates that the proposed method can be generated the virtual battlefield situation dataset.

Propolis from the Stingless Bee Trigona incisa from East Kalimantan, Indonesia, Induces In Vitro Cytotoxicity and Apoptosis in Cancer Cell lines

  • Kustiawan, Paula M;Phuwapraisirisan, Preecha;Puthong, Songchan;Palaga, Tanapat;Arung, Enos T;Chanchao, Chanpen
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.15
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    • pp.6581-6589
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    • 2015
  • Background: Previously, stingless bee (Trigona spp.) products from East Kalimantan, Indonesia, were successfully screened for in vitro antiproliferative activity against human cancer derived cell lines. It was established that propolis from T. incisa presented the highest in vitro cytotoxicity against the SW620 colon cancer cell line (6% cell survival in $20{\mu}g/mL$). Materials and Methods: Propolis from T. incisa was extracted with methanol and further partitioned with n-hexane, ethyl acetate and methanol. The in vitro cytotoxicity of the extracts was assessed by the MTT assay against human colon (SW620), liver (Hep-G2), gastric (KATO-III), lung (Chago) and breast (BT474) cancer derived cell lines. The active fractions were further enriched by silica gel quick column, absorption and size exclusion chromatography. The purity of each fraction was checked by thin layer chromatography. Cytotoxicity in BT-474 cells induced by cardanol compared to doxorubicin were evaluated by MTT assay, induction of cell cycle arrest and cell death by flow cytometric analysis of propidium iodide and annexin-V stained cells. Results: A cardol isomer was found to be the major compound in one active fraction (F45) of T. incisa propolis, with a cytotoxicity against the SW620 ($IC_{50}$ of $4.51{\pm}0.76{\mu}g/mL$), KATO-III (IC50 of $6.06{\pm}0.39{\mu}g/mL$), Hep-G2 ($IC_{50}$ of $0.71{\pm}0.22{\mu}g/mL$), Chago I ($IC_{50}$ of $0.81{\pm}0.18{\mu}g/mL$) and BT474 (IC50 of $4.28{\pm}0.14{\mu}g/mL$) cell lines. Early apoptosis (programmed cell death) of SW620 cells was induced by the cardol containing F45 fraction at the $IC_{50}$ and $IC_{80}$ concentrations, respectively, within 2-6 h of incubation. In addition, the F45 fraction induced cell cycle arrest at the G1 subphase. Conclusions: Indonesian stingless bee (T. incisa) propolis had moderately potent in vitro anticancer activity on human cancer derived cell lines. Cardol or 5-pentadecyl resorcinol was identified as a major active compound and induced apoptosis in SW620 cells in an early period (${\leq}6h$) and cell cycle arrest at the G1 subphase. Thus, cardol is a potential candidate for cancer chemotherapy.

DFT 방법을 이용한 벤젠 삼합체 π-π interaction의 양자역학 계산

  • Jeong, Hyeon-Su;Park, Gi-Cheol;Cho, Art.
    • Proceeding of EDISON Challenge
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    • 2014.03a
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    • pp.399-408
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    • 2014
  • 신약을 개발하거나 단백질 구조를 예측하는데 Molecular Mechanics (MM)의 방법을 사용한다. 하지만, MM 만으로는 자연현상에서 일어나는 결과를 정확하게 기술하기 어렵다. 본 연구는 기존의 MM 방법으로는 정확히 예측이 불가능한 비 공유결합 중 하나인 ${\pi}-{\pi}$ interaction을 양자역학 계산을 통해 정확한 예측이 가능한지 알아보았다. ${\pi}-{\pi}$ interaction이란 생채 내, 의약 화합물에서 발견되는 결합이기 때문에, 단백질과 결합하는 구조의 예측에 중요하다고 할 수 있다. 본 실험은 ${\pi}-{\pi}$ interaction을 갖는 Sandwich, T shape, 그리고 Parallel displaced 세 가지 모형과 각각의 모형 아래에 분자를 하나 더 쌓은 모형을 추가하여 양자역학 재산을 수행하였다. 양자역학 계산은 DFT의 세가지 함수 M06_2X, M05_2X, B3LYP를 이용하였다. 실험결과에서 세 가지 함수가 각기 다른 결과를 보였는데, 상대적으로 B3LYP의 경우에는 세가지 모델에서 모두 제대로 된 에너지 변화를 계산하지 못하였으며, M06_2X와 M05_2X의 결과에서는 거리에 따른 ${\pi}-{\pi}$ interaction 에너지의 변화를 정확하게 계산하였다. 이러한 결과를 바탕으로, 양자역학의 방법을 통해 MM에서는 예측이 불가능한 ${\pi}-{\pi}$ interaction을 계산 할 수 있고 이 부분을 고려하여 화합물 간의 결합구조를 예측을 향상시킬 수 있다.

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