• Asian-Australasian Journal of Animal Sciences
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• 제15권7호
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• pp.932-937
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• 2002

A partial genome scan using porcine microsatellites was carried out to detect quantitative trait loci (QTL) for backfat thickness (BFT) in a pig reference population. This population carried QTL on chromosomes 1, 13 and 18. The QTL on chromosome 1 was located between marker loci S0113 and SW1301. The QTL corresponded to very low density lipoprotein receptor gene (VLDLR) in location and in biological effects, suggesting that VLDLR might be a candidate gene. The QTL found on chromosome 13 was found between marker loci SWR1941 and SW864, but significance for the marker-trait association was inconsistent by using data with different generations. The QTL on chromosome 18 was discovered between markers S0062 and S0117, and it was in proximity of the regions where IGFBP3 and GHRHR were located. The porcine obese gene might be also a candidate gene for the QTL on chromosome 18. In order to understand genetic architecture of BFT better, fine mapping and positional comparative candidate gene analyses are necessary.

• 한국응용곤충학회지
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• 제37권2호
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• pp.187-191
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• 1998

새로운 Bacillus thuringiensis NT0423균주를 이용한 배양체계를 확립하고 대량생산을 위한 새로운 배양배지를 개발하였다 대두박과 밀기울의 다양한 성분비로 구성된 5종의 SW 배지는 기존의 인공합성배지인 GYS나 LB B. huringiensis 의 성장과 포자형성면에서 훨씬 우수한 결과를 보였고, 그중 대두박과 밀기율이 3:2의 비율로 구성된 SW32배지에서 전체 세포수가 3.9$\times$${10}^{8}$CFU/ml에 달했고, 대부분의 세포가 빠르게 포자를 형성하여 (3.7$\times$${10}^{8}$CFU/ml)가장 효율적이었다. 배양에 따른 배지의 pH는 최저 6.2에서 최고 7.3까지 변화하여 성장에 크게 영향을 미치지 않았고, 전체 배양액에서 대두박과 밀기울이 차지하는 비율이 4%일 경우, ml당 4.2$\times$${10}^{8}$CFU/ml의 포자가 형성되어 B. thuringiensis 의 성장에 가장 유리하였다. 교반플라스크와 5ι의 소규모 발효기를 이용한 B. thuringiensis 의 배양조건 확립에 의해 각각 최대 1$\times$${10}^{9}$CFU/ml과 5$\times$${10}^{10}$CFU/ml에 해당하는 많은 양의 균체를 회수할 수 있다.

• 미생물학회지
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• 제50권4호
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• pp.302-307
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• 2014

많은 근권세균들이 다양한 직간접적인 방법을 통해 식물생장을 촉진할 수 있으며 indole acetic acid (IAA) 같은 식물호르몬의 생산과 분비는 식물의 생장과 발달에 큰 영향을 미친다. 강아지풀의 근권에서 분리된 Acinetobacter sp. SW5는 BHB 배지에서 각각 2일과 1일 배양 시 118.1 mg/L의 IAA와 4.5 mg/L의 지베렐린을 생성하였다. 소규모 재배실험에서 토마토 유묘에 이 균주를 2주 간격으로 처리하면서 12주 재배 시 토마토 식물의 shoot와 뿌리 길이 및 습윤중량이 비접종 대조군과 비교할 때 통계적으로 유의하게 각각 26.3, 33.3과 105.3% 증가하였다. 토마토 유묘의 뿌리로부터 분비되는 삼출물을 HPLC로 분석한 결과 IAA의 전구물질인 아미노산 트립토판이 3.75 ng/mg tomato root 검출되었으며 Acinetobacter sp. SW5는 8개의 토마토 유묘뿌리 삼출물로부터 $4.06{\mu}M$의 IAA를 생성하였다. 토마토 뿌리 삼출물에서 Acinetobacter sp. SW5의 생장능과 더불어 이 세균에 의해 생성된 IAA가 토마토 식물의 생장을 촉진시키는데 관여했을 것으로 추정된다.

• 유기물자원화
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• 제25권3호
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• pp.55-62
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• 2017

본 연구는 해조류 첨가를 통한 음식물쓰레기의 소화효율 증대효과를 알아보기 위해 다양한 농도(2.5~10.0 g VS/L)와 혼합비율(FW:SW=100:0, 75:25, 50:50, 25:75, 0:100, VS 농도 기준)에서 회분식 실험을 수행하였다. 음식물쓰레기의 단일소화의 경우 농도가 증가함(2.5~10.0 g VS/L)에 따라 메탄전환율이 394, 377, 276, $49mL\;CH_4/g\;VS_{added}$로 감소하는 경향을 보였다. 음식물쓰레기에 해조류의 첨가 비율이 높아질수록 낮은 기질농도(2.5~5.0 g VS/L)에서는 메탄전환율이 감소하였으나(최대 15% 감소) 높은 기질농도(7.5~10.0 g VS/L)에서는 메탄전환율이 증가하였다(최대 240% 상승). 또한 음식물쓰레기와 해조류의 단일소화 시 발생된 메탄가스의 양을 기반으로 상승효과에 의한 메탄발생량을 계산한 결과, 2.5, 5.0 g VS/L에서는 상승효과가 없었고, 7.5, 10.0 g VS/L의 경우에는 해조류의 비율이 높아질수록 상승효과가 최대 25 % (= 상승효과에 의한 메탄발생량/혼합소화 시 실제 발생한 메탄발생량, 기질농도 10.0 g VS/L, 혼합비율 50:50)까지 상승하였다. 음식물쓰레기의 농도가 10.0 g VS/L로 높은 경우 단일소화 시(FW=SW=100:0) 유기산 축적량이 7,426 mg COD/L까지 증가하였고, 해조류를 첨가하면(FW:SW=50:50) 유기산 축적량이 2,346 mg COD/L로 감소하였다. 이는 비교적 생분해도가 낮은 해조류를 첨가함으로서 유기산 생산속도의 조절을 통해 유기산이 축적되는 것을 억제하여 상승효과가 발생한 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권8호
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• pp.1054-1058
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• 2000

The objective of this study was to determine the effect of draught stress on certain haemograms and ovarian activity and the influence of dietary supplementation on the negative effects of draught in cows. Blood parameters and ovarian activity were assessed in supplemented non-working (SNW), supplemented working (SW) and non-supplemented non-working (NSNW) cows. Body weights and body condition scores were recorded fortnightly. Blood samples were collected through jugular venipuncture in December, February and April to determine the contents of haematocrit, erythrocyte, haemoglobin and white blood cells. Ovarian palpations were carried out in October, January and April. The SW cows maintained body weights (p>0.05) during the monitoring period whereas both SNW and NSNW cows gained (p<0.05) body weights. Body condition scores were similar between SW and NSNW cows. Cows in the NSNW group had lower (p<0.05) haematocrit concentrations in April than both supplemented groups. In December, erythrocyte concentrations were similar (p>0.05) among all treatment groups. Haemoglobin concentrations were higher (p<0.05) in SW and SNW cows in February and April than in December. The SW cows had higher leucocyte contents (p<0.05) in February than the other groups of cows. All treatment groups showed similar (p>0.05) ovarian activity in January. However, the NSNW group showed a lower proportion (p<0.05) of cows that exhibited normal ovarian activity in April. The results suggest that dietary supplementation of cows increases haematocrit and haemoglobin contents. In addition, supplementary feeding during the period of draught power provision maintains ovarian activity in cows.

• 원예과학기술지
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• 제33권5호
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• pp.730-736
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• 2015

Tomato spotted wilt virus (TSWV) causes one of the most destructive viral diseases that threatens global tomato production. Sw-5b was reported as the resistance gene effective against TSWV. The objective of this research was to develop a single-nucleotide polymorphism (SNP) marker to distinguish tomato cultivars resistant to TSWV from susceptible cultivars for marker-assisted breeding. First, we determined genotypes for TSWV resistance in 32 commercial tomato cultivars using the previously reported Sw-5b gene-based marker. Then, DNA sequences of Sw-5b alleles in tomato cultivars showing resistant or susceptible genotypes were analyzed; a single SNP was found to distinguish tomato cultivars resistant to TSWV from susceptible cultivars. Based on the confirmed SNP, a SNP primer pair was designed. Using this new SNP sequence and high-resolution melting analysis, the same 32 tomato cultivars were screened. The results were perfectly correlated with those from screening with the Sw-5b gene-based marker. These results indicate that the SNP maker developed in this study will be useful for better tracking of resistance to TSWV in tomato breeding.

• 펄프종이기술
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• 제48권3호
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• pp.37-43
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• 2016

Various mechanical and chemical pretreatment methods including alkali treatment, pre-beating, enzyme treatment and oxidation treatment have been used to reduce the production energy of the microfibrillated cellulose (MFC). Among them, alkali swelling can be helpful to reduce the energy consumption because the internal bonding between fibrils could be weakened. In this study, dimethyl sulfoxide (DMSO) was used as a cosolvent to improve alkali pretreatment efficiency and the effects of NaOH concentration during NaOH-DMSO swelling on changes in fiber characteristics of softwood bleached kraft pulp (SwBKP) were elucidated. For alkali treatment in H2O-DMSO solvents, fiber length were decreased with increasing NaOH concentration while fiber width, curl and WRV were increased. WRV began to increase at 8% NaOH solution. In addition, above 8% concentration of NaOH, crystalline structure of pulp fibers converted from cellulose II to cellulose III by DMSO cosolvent. Comparing the previous results with this study, it was shown that DMSO cosolvent could promote swelling of pulp fibers and thus reduce NaOH concentration for the maximum swelling of fibers.

• 치과방사선
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• 제28권1호
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• pp.127-143
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• 1998

The author evaluated the effects of taxol, a microtubular inhibitor, as a possible radiation sensitizer and the production of prostaglandins on three human cancer cell lines(KB, RPMI-2650 and SW-13) and one murine cell line(L929). Each cell line was divided into four groups (control, taxol only, radiation only and combination of taxol and radiation). The treatment consisted of a single irradiation of 10Gy and graded doses (5, 50, 100, 200, 300, 500 nM) of taxol for a 24-h period. The cytotoxicity of taxol alone was measured at 1 day after(1-day group) and 4 days after(4-day group) the treatment. The survival ratio of cell was analyzed by MTT (3-(4,5-dimethylthiazol-2-yl) -2,5-dimethyl tetrazolium bromide) test. Prostaglandins(PGE2 and PGI2) were measured in the culture medium by a radioimmunoassay. The results obtained were as follows. 1. There was a significantly increased cytotoxicity of KB cells in 4-day group than those in I-day group. There was a high correlation between doses of taxol and cell viability in both groups(l-day group R=0.82741, 4-day group R=0.84655). 2. There was a significantly increased cytotoxicity of RPMI -2650 cells treated with high concentration of taxol in 4-day group than those in I-day group. Also there was a high correlation between doses of taxol and cell viability in 4-day group(R=0.93917). 3. There was a significantly increased cytotoxicity of SW-13 cells treated with high concentration of taxol in 4-day group than those in 1-day group. However no high correlation was observed between doses of taxol and cell viability in both groups(1-day group R=0.46362, 4-day group R=0.65425). 4. There was a significantly increased cytotoxicity of L929 cells treated with low concentration of taxol in 4-day group than those in 1-day group. At the same time, there was a low correlation between doses of taxol and cell viability in both groups(1-day group R=0.34237, 4-day group R=0.23381). 5. In I-day group of L929 cells, higher cytotoxicities were observed in the groups treated with 500 nM taxol than given 10 Gy radiation alone. L929 cells in I-day group alone showed a radiosensitizing effect by taxol.. 6. In addition to L929 cells, all cancer cells treated with a combination of taxol and radiation in 4-day group appeared to have some fragmented nuclei and to float on the medium. In addition, L929 cells appeared to be more confluent. 7. The level of PGE2 production was the highest in the contol KB cells. This appeared to increase in every experimental group of all three cancer cells except L929 cells. There was a significantly increased production of PGE2 in SW -13 cells treated with a combination taxol and radiation compared to the other experimental groups. 8. The level of PGE2 production in the control group of RPMI-Z650 cells was the highest. This appeared to increase in every experimental group of all cells except in SW-13 cells. This also increased significantly in RPMI-2650 cells treated with a combination of taxol and radiation compared to the other experimental groups.

• Journal of Microbiology and Biotechnology
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• 제25권6호
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• pp.903-909
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• 2015

Indole-3-acetic acid (IAA) production is a typical mechanism of plant growth promotion by some rhizobacteria. However, a functional genomic study is necessary to unravel the function and mechanism of IAA signaling during rhizobacteria-plant interactions. In this study, the expression of SlIAA1 and SlIAA9 among the auxin response genes in tomato was examined during the interaction between IAA-producing Acinetobacter guillouiae SW5 and tomato plants. When 3-day grown tomato seedlings were treated for 30 min with 10~100 µM of IAA produced by bacteria from tryptophan, the relative mRNA levels of SlIAA1 and SlIAA9 increased significantly compared with those of the control, demonstrating that IAA produced by this bacterium can induce the expressions of both genes. Inoculation of live A. guillouiae SW5 to tomato seedlings also increased the expressions of SlIAA1 and SlIAA9, with more mRNA produced at higher bacterial density. In contrast, treatment of tomato seedlings with dead A. guillouiae SW5 did not significantly affect the expression of SlIAA1and SlIAA9. When 3-day bacterial culture in tomato root exudates was administered to tomato seedlings, the relative mRNA level of SlIAA1 increased. This result indicated that the plant may take up IAA produced by bacteria in plant root exudates, which may increase the expression of the auxin response genes, with resulting promotion of plant growth.

• 전자공학회논문지SC
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• 제47권6호
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• pp.19-26
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• 2010

본 연구는 국내 무기체계 양산단계에서 적용되고 있는 ATE 장비의 SW 신뢰성 확보 및 양산단계에서의 관리를 위한 방안을 제안하였다. 국내 무기체계 획득분야 중 양산단계 ATE 장비의 개발 관리에 대한 프로세스 정립이 미흡하여 이를 개선을 위해 ATE의 정의에서부터 ATE 장비 신뢰성 확보 및 관리 방안의 선진국 사례, 국내 현황 등을 살펴보고 국내 현실에 맞는 ATE 장비의 신뢰성 검증 방안 및 개발 관리 분야에서 개선해야 할 점을 살펴보았다. ATE 장비의 신뢰성을 확보하기 위해서는 방산업체에서는 각종 ATE 개발 프로세스의 구축이 필요하며, 정부 품질보증기관은 양산단계 ATE 개발 관리를 위해 ATE 장비 개발 시 단계별 위험요소 식별, 위험관리 활동 및 개발완료단계에서 품질평가 활동을 통해 ATE 장비의 신뢰성을 확보해야 할 것이다.