• 폴리머
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• 제38권2호
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• pp.250-256
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• 2014

본 연구에서는 이축 스크류식 압출기를 이용하여 폴리프로필렌(PP)에 도전성 필러인 다중벽 탄소나노튜브 (MWNT)와 스테인레스강 단섬유(SSF)를 첨가하여 복합체를 제조하였으며, 이에 대한 표면저항 및 기계적 특성을 조사하였다. 표면저항을 측정한 결과 PP/MWNT에 소량의 SSF를 첨가하였을 때 더 낮은 MWNT 함량에서 percolation threshold가 나타났다. 그리고 제조된 복합체에 대한 인장시험 결과 순수 PP와 비교해서 파괴점 신장률은 감소하였으나 탄성률과 강도는 증가하였다. 또한 동역학분석을 통하여 MWNT와 SSF 복합체의 저장탄성률과 tan ${\delta}$에 미치는 영향을 조사하였으며, SEM을 이용하여 필러들의 모폴로지 및 복합체의 파단면을 관찰하였다.

• 농촌지도와개발
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• 제19권4호
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• pp.961-987
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• 2012

본 연구는 강소농의 개념 정립을 통해 사과를 대상으로 강소농의 공간적 분포특성과 결정요인을 분석하였으며, 2010년 농림어업총조사 자료를 활용하였다. 공간적 분포특성은 공간 클러스터링 기법을 이용하여 과수 강소농 가구가 어느 지역에 집중적으로 분포하는지를 분석하였다. 강소농과 비강소농의 이산적인 형태의 종속변인을 구성한 후, 프로빗 모형을 활용, 인구 경제적, 경영 특성 등의 독립변인을 통제한 상태에서 사과 강소농의 결정요인을 분석하였다. 분석결과, 2010년 현재 사과 강소농은 모두 1,529가구로 분석되었으며, 이들 가구들은 경상북도에 밀집하여 분포하는 것으로 드러났다. 강소농의 결정요인은 농가의 소득 결정요인과 유사한 결과를 보였다. 사과 주산지에 입지하고 있고, 정보화가 높은 농가일수록, 그리고 생산자 조직에 참여하고 있으며, 직접 판매를 하는 농가일수록 강소농일 확률이 높게 나타났다. 본 연구의 분석결과는 강소농 정책을 육성하는데 있어 반드시 요구되는 강소농의 특성을 파악하였다는 측면에서 향후 강소농 육성 정책에 다양한 기초자료를 제공할 수 있으리라 판단된다.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.598-606
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• 2001

A mathematical model is proposed that can depict the kinetics of simultaneous saccharification and fermentation (SSF) using steam-exploded wood(SEW) with a glucose- and cellobiose-fermenting yeast strain. Brettanomyces custersii. An expression to describe the reduction of the relative digestibility during the hydrolysis of the SEW is introduced in the hydrolysis model. The fermentation model also takes two new factors into account, that is, the effects of the inhibitory compounds present in the SEW hydrolysates on the microorganism and the fermenting ability of Brettanomyces custersii, which can use both glucose and cellobiose as carbon sources. The model equations were used to simulate the hydrolysis of the SEW, the fermentation of the SEW hydrolysates, and a batch SSF, and the results were compared with the experimental data. The model was found to be capable of representing ethanol production over a range of substrate concentrations. Accordingly, the limiting factors in ethanol production by SSF under the high concentration of the SEW were identified as the effect of inhibitory compounds present in the SEW, the enzyme deactivation, and a limitation in the digestibility based on the physical condition of the substrate.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1161-1168
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• 2009

Ethanol production by the simultaneous saccharification and fermentation (SSF) of low-value rice wine cake (RWC) without cooking was investigated. RWC is the filtered solid waste of fermented rice wine mash and contains 53% raw starch. For the SSF, the RWC slurry was mixed with the raw-starch-digesting enzyme of Rhizopus sp. and yeast, where the yeast strain was selected from 300 strains and identified as Saccharomyces cerevisiae KV25. The highest efficiency (94%) of ethanol production was achieved when the uncooked RWC slurry contained 23.03% starch. The optimal SSF conditions were determined as 1.125 units of the raw-starch-digesting enzyme per gram of RWC, a fermentation temperature of $30^{\circ}C$, slurry pH of 4.5, 36-h-old seeding culture, initial yeast cell number of $2{\times}10^7$ per ml of slurry, 17 mM of urea as the nitrogen additive, 0.25 mM of $Cu^{2+}$ as the metal ion additive, and a fermentation time of 90 h. Under these optimal conditions, the ethanol production resulting from the SSF of the uncooked RWC slurry was improved to 16.8% (v/v) from 15.1% (v/v) of pre-optimization.

• 농촌지도와개발
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• 제22권3호
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• pp.261-268
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• 2015

The Strong Small Farm (SSF) is small farm, but strong farm so that the SSF competes with farm of the world. Also, SSF means that farmer brings about management innovation through improvements of continuous competence and individual efforts. Ultimate goals of SSF are to improve competence of farm household by 20 percent and to increase farm household income by 10 percent. The purpose of this paper is to examine the factors affecting the improvement of competence of the Strong Small Farm. The major findings of this study are summarized as follows. First, the results indicated that competence of farm household was improved by 8.5 percent. Second, the findings showed that management plan report, implement report, precision management consulting and whether or not he or she was urban to rural returner had a significant impact on improvement of competence.

• KSBB Journal
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• 제30권3호
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• pp.125-131
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• 2015

Kluyveromyces marxianus is a well-known thermotolerant yeast. Although Saccharomyces cerevisiae is the most commonly used yeast species for ethanol production, the thermotolerant K. marxianus is more suitable for simultaneous saccharification and fermentation (SSF) processes. This is because enzymatic saccharification usually requires a higher temperature than that needed for the optimum growth of S. cerevisiae. In this study, we compared the fermentation patterns of S. cerevisiae and K. marxianus under various temperatures of fermentation. The results show that at a fermentation temperature of $45^{\circ}C$, K. marxianus exhibited more than two fold higher growth rate and ethanol production rate in comparison to S. cerevisiae. For SSF using starch or corn stover as the sole carbon source by K. marxianus, the high temperature ($45^{\circ}C$) fermentations showed higher enzymatic activities and ethanol production compared to SSF at $30^{\circ}C$. These results demonstrate the potential of the thermotolerant yeast K. marxianus for SSF in the industrial production of biofuels.

• KSBB Journal
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• 제18권1호
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• pp.14-18
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• 2003

본 연구에서는 제지 슬러지를 이용한 SSF 공정에 L..paracasei KLB58을 적용하여 젖산과 더불어 생균제용 균체를 경제적으로 대량 생산하고자 하였다. KLB58의 배양온도와 섬유소 가수분해 효소인 $\beta$-glucosidase의 농도를 조절하여 최적의 생산 조건을 확인해 본 결과, 37$^{\circ}C$ 에서 2 unit/ml의 $\beta$-glucosidase를 첨가하여 배양하였을 때 최대의 젖산과 균체를 생산하였다. 또한 $\beta$-glucosidase를 포함하지 않아도 상대적으로 많은 양의 젖산과 균체를 생산하였으므로, 이에 대한향후 연구가 기대된다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 The 6th International Symposium of East Asian Resources Recycling Technology
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• pp.106-110
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• 2001

Formation of stoichiometric lithium-, nickel-, and zinc- ferrites by calcining organo-metallic precursors a temperature below 40$0^{\circ}C$ is examined using DTA/TG, and XRD techniques. It attempts to simulate th immobilization of metal ions in industrial liquid influents (waste) through the synthesis of stoichiometric spinel ferrites (SSF). Two steps of the SSF formation during thermal treatments are noted. The transformation of magnetite to ${\gamma}$ - Fe$_2$O$_3$and subsequent first formation of SSF were observed at temperatures ranging from 200 to 45$0^{\circ}C$. Th formation of cation-containing ${\gamma}$-Fe$_2$O$_3$and subsequent second formation of the ferrite occurred at temperature ranges of < 45$0^{\circ}C$ and 500 to $650^{\circ}C$, depending on the heating rate used. Then the temperature range of 200t 45$0^{\circ}C$ is critical to the performance of the technique, because a calcination at the range would lead to a complete formation of SSF, avoiding the occurrences of ${\gamma}$-Fe$_2$O$_3$and ion-containing ${\gamma}$-Fe$_2$O$_3$. If not, so $\alpha$-Fe$_2$O$_3$would occur. And annealing at temperature above $650^{\circ}C$ must be employed by which solid-state reactio of $\alpha$-Fe$_2$O$_3$with metal ions (possibly metal oxides) to form SSF can be conducted.

• Journal of the Korean Wood Science and Technology
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• 제39권6호
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• pp.490-497
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• 2011

본 연구는 옥살산으로 전처리를 수행한 후 얻어진 옥수숫대를 이용하여 동시당화발효를 위한 최적조건을 탐색하였다. Pichia stipitis CBS 6054를 이용한 동시당화발효에서 독립변수인 반응온도($25.8{\sim}34.2^{\circ}C$)와 교반속도(80~220 rpm)에 대한 에탄올 생산량은 각각 99% 신뢰구간을 가졌다. 종속변수로 에탄올 생산량을 적용하였을 때 $30^{\circ}C$, 170 rpm에서 최대의 에탄올 생산을 예측할 수 있었다(22.5 g/L). 최적의 온도 및 교반속도에서 최적 질소원을 조사한 결과 yeast extract (1.25 g/L)와 urea (1.25 g/L)를 혼합하여 사용하였을 경우 에탄올 생산량은 증가하였으며 trace metal 성분과 비타민은 첨가하지 않았을 때 에탄올 생산이 촉진되었다. 동시당화 발효를 위한 $KH_2PO_4$, $MgSO_4{\cdot}7H_2O$의 최적 농도는 각각 1 g/L, 0.25 g/L로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제10권6호
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• pp.770-775
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• 2000

Several process parameters were studied to ascertain the effect on degradation of sugar cane bagasse in relation to the production of cellulase enzyme and reducing sugars by Solid Substrate Fermentation (SSF) and Submerged Culture Fermentation (SCF) of Aspergillus terreus SUK-1. The effect of air-flow rate (0-1.3 v/v/m), of different ratios of substrate weight to liquid volume (1:6, 1:10, 1:20, and 1:30 w/v, g/ml), scale-up effect (10, 20, and 100 times of 1:10 ration, w/v) and the effect of temperature (30, 40, 50, and $60^{\circ}C$) in SSF were studied. Air-flow rate of 1.0 v/v/m gave the highest enzyme activity (FPase 0.25 IU/ml, CMCase 1.24 IU/ml) and reducing sugars concentration (0.72 mg/ml). Experiment using 1:10 ratio (w/v) was found to support maximum cellulase activity (FPase 0.58 IU/ml, CMCase 1.97 IU/ml) and reducing sugar concentration (1.23 mg/ml). Scaling-up the ratio of 1:10(w/v) by a factor of 20 gave the highest cellulase activity (FPase 0.71 IU/ml, CMCase 2.25 IU/ml) and reducing sugar concentration (3.67 mg/ml). The optimum temperature for cellulase activity and reducing sugar production was $50^{\circ}C$(FPase 0.792 IU/ml, CMCase 2.25 IU/ml and 3.85 mg/ml for reducing sugar concentration). For SCF, the activity of cellulase enzyme and reducing sugar concentration was found to be lower than that obtained for SSF. The highest cellulase activity obtained in SCF was 50% lower than the highest cellulase activity in SSF, while for reducing sugar concentration, the highest concentration obtained in SCF was 90% lower than that obtained in SSF.