• Journal of ILASS-Korea
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• v.26 no.4
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• pp.212-218
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• 2021

The present study aims to develop the Surface Plasmon Resonance (SPR) imaging system facilitating the real-time measurement of the concentration of evaporating binary mixture droplet (BMD). We introduce the theoretical background of the SPR imaging technique and its methodology for concentration measurement. The SPR imaging system established in the present study consists of a LED light source, a polarizer, a lens, and a band pass filter for the collimated light of a 589 nm wavelength, and a CCD camera. Based on the Fresnel multiple-layer reflection theory, SPR imaging can capture the change of refractive index of evaporating BMD. For example, the present study exhibits the visualization process of ethylene glycol (EG)-water (W) BMD and measures real-time concentration change. Since the water component is more volatile than the ethylene glycol component, the refractive index of EG-W BMD varies with its mixture composition during BMD evaporation. We successfully measured the ethylene glycol concentration within the evaporating BMD by using SPR imaging.

• Proceedings of the Korea Institutes of Information Security and Cryptology Conference
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• 2003.12a
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• pp.655-659
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• 2003

시스템의 안전성을 평가하기 위해 안전성 문제 해결 도구인 SPR［1］을 이용하여 보안 모델을 프롤로그 기반의 명세 언어인 SPSL로 기술하여 안전성을 검증한다. 보안 모델은 시스템의 3가지 컴포넌트, 시스템 보안 상태(system security states), 접근 통제 규칙(access control rules), 그리고 보안 기준(security criteria)으로 구성된다. 본 논문에서는 보안 모델의 보안 기준에 NTFS의 다중 사용 권한을 적용하여 명세하고 안전성 문제 해결방법을 제시하고자 한다.

• Food Science and Biotechnology
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• v.17 no.1
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• pp.15-19
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• 2008

This research was conducted to develop a quick and sensitive method of detecting carbamate residues using the immobilization of antibody-antigen interactions with surface plasmon resonance (SPR). We have used commercialized surface plasmon resonance equipment (Biacore 3000). The antibody used for the immunoassay was specific for glutathione-s-transferase (GST) and the antigens included several carbamate pesticides (carbofuran, carbaryl, and benfuracarb). When antigens were applied to the protein GST, the detection limit was 2 ng/mL of carbamate pesticide. The fabricated protein GST maintained its activity for over 200 measurements. Thus we determined that the SPR biosensors could detect the specific reversible binding of a reactant in solution to a binding partner immobilized on the surface of the sensor and allow real-time detection and monitoring.

• 기계와재료
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• v.23 no.3
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• pp.138-146
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• 2011

자동차 차체 경량화를 위해 알루미늄 스페이스 프레임 구조가 개발되고 있으나, 관재결합이 필요하기 때문에 기존의 저항 점용접이 적용되기 어렵다. 또한, 멤버와 멤버의 연결부에서는 철강재난 고강도 재료의 사용이 요구되므로 이종재료 접합기술이 필요하다. 알루미늄 및 이종재료 접합방법으로는 볼트체결, 클린칭, SPR 접합, 접착재 등이 있으나, SPR 접합은 기계적인 결합방법의 하나로, 일반 리벳공정과는 달리 별도의 홀이 필요없기 때문에 자동화에 용이하며 작업시간도 빠르다. 리벳의 압입 방식으로 판재의 열변형이 거의 없고 친환경적인 공법으로 사용되고 있으며, 소음이 적고, 용접이 불가능한 이종재료의 결합도 가능하다. 무엇보다 자동차 양산용 장비 적용이 용이하기 때문에 기존의 저항 점용접을 대체하기 편리하다. 따라서, 본 글에서는 알루미늄 차체 부품 접합을 위한 SPR 접합공법에 대한 국내외 기술개발 동향을 분석하고, 한국기계연구원에서의 최근 기술개발 내용을 소개하고자 한다.

• Journal of Sensor Science and Technology
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• v.25 no.4
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• pp.243-246
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• 2016

The characteristics of a bimetallic surface plasmon resonance (SPR) chip were investigated to detect a tumor biomarker, carcinoembryonic antigen (CEA). The linewidth and the tangential slope of the reflectance curve of the bimetallic SPR chip was compared with those of the reflectance curve of a conventional gold (Au) SPR chip. The changes in reflectance in response to the variation in CEA in the critical concentration range were analyzed at an angle where the tangential slope of the reflectance curve was maximum. From linear regression analysis, the sensitivity of the bimetallic SPR chip with respect to the CEA in critical concentration was obtained.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.4
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• pp.795-800
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• 2009

Surface plasmon resonance (SPR) has been widely used for biological and chemical sensing applications. The present study investigates numerically the optical characteristics for the single Au film and bimetallic Ag/Au film SPR configurations by using the multiple beam interference matrix (MBIM) method. We use the prism coupling method, especially Kretschmann configuration for excitation of surface plasmon wave (SPW). The estimated results of reflectance, phase shift and magnetic field intensity enhancement factor are provided for finding out the optimum configuration with high sensitivity for SPR measurement. As a result, the optimum thicknesses are found to be 52 nm for a single Au film and 5 nm to 36 nm for bimetallic Ag-Au film. From the comparison of full width half maximum (FWHM) values for reflectance, phase shift, and enhancement of magnetic field intensity, it is concluded that the highest sensitivity can be obtained when using the phase shift for SPR sensor.

• Journal of Microbiology and Biotechnology
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• v.17 no.6
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• pp.1031-1035
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• 2007

Prostate specific $antigen-{\alpha}_1-antichymotrypsin$ was detected by a double-enhancement strategy involving the exploitation of both colloidal gold nanoparticles (AuNPs) and precipitation of an insoluble product formed by HRP-biocatalyzed oxidation. The AuNPs were synthesized and conjugated with horse-radish peroxidase-PSA polyclonal antibody by physisorption. Using the protein-colloid for SPR-based detection of the PSPJACT complex showed their enhancement as being consistent with other previous studies with regard to AuNPs enhancement, while the enzyme precipitation using DAB substrate was applied for the first time and greatly amplified the signal. The limit of detection was found at as low as 0.027 ng/ml of the PSA/ACT complex (or 300 fM), which is much higher than that of previous reports. This study indicates another way to enhance SPR measurement, and it is generally applicable to other SPR-based immunoassays.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.11a
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• pp.778-783
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• 2003

The problem considered in this paper is about the collocation of sensor and actuator for the active control of sound and vibration. It is well-known that a point collocated sensor-actuator pair offers an unconditional stability with very high performance when it is used with a direct velocity feedback (DVFB) control, because the pair has strictly positive real (SPR) property. In order to utilize this SPR characteristics, a matched piezoelectric sensor and actuator pair is considered, but this pair suffers from the in-plane motion coupling problem with the out-of$.$plane motion due to the piezo sensor and actuator interaction. This coupling phnomenon limits the stability and performance of the matched pair with DVFB control. As a new alternative, a point sensor and piezoelectric actuator pair is also considered, which provides SPR property in all frequency range except at the first resonance in very low frequency. This non-SPR resonance could be minimized by applying a phase lag compensator.

• Korean Journal of Veterinary Service
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• v.30 no.2
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• pp.205-209
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• 2007

A protein chip based on surface plasmon resonance (SPR) imaging was developed for measuring classical swine fever virus (CSFV) antibody using a recombinant gp55 protein as an antigen. The diagnostic potential of SPR imaging for detecting antibodies to the CSFV gp55 protein was compared with that of a enzyme -linked immunosorbent assay (ELISA) using 70 pig sera. There was a strong positive correlation between the SPR imaging and ELISA (n=70, r=0.916, p<0.01). Therefore, the SPR imaging, which is a label-free and high-through put method, is expected to be a valuable tool in the serodiagnosis of CSFV.

• Proceedings of the KSME Conference
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• 2005.11a
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• pp.85.2-85.2
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• 2005