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SPR-based Antibody-Antigen Interaction for Real Time Analysis of Carbamate Pesticide Residues  

Yang, Gil-Mo (Department of Biological Engineering, University of Missouri-Columbia)
Kang, Suk-Won (National Institute of Agricultural Engineering, Rural Development Administration)
Publication Information
Food Science and Biotechnology / v.17, no.1, 2008 , pp. 15-19 More about this Journal
Abstract
This research was conducted to develop a quick and sensitive method of detecting carbamate residues using the immobilization of antibody-antigen interactions with surface plasmon resonance (SPR). We have used commercialized surface plasmon resonance equipment (Biacore 3000). The antibody used for the immunoassay was specific for glutathione-s-transferase (GST) and the antigens included several carbamate pesticides (carbofuran, carbaryl, and benfuracarb). When antigens were applied to the protein GST, the detection limit was 2 ng/mL of carbamate pesticide. The fabricated protein GST maintained its activity for over 200 measurements. Thus we determined that the SPR biosensors could detect the specific reversible binding of a reactant in solution to a binding partner immobilized on the surface of the sensor and allow real-time detection and monitoring.
Keywords
biosensor; surface plasmon resonance (SPR); carbamate; pesticide residue;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
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