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Development and evaluation of surface plasmon resonance imaging for the detection of antibodies against classical swine fever virus in swine  

Cho, Ho-Seong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University)
Lee, Tae-Uk (Jeoanam Livestock and Veterinary Science)
Park, Nam-Yong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University)
Publication Information
Korean Journal of Veterinary Service / v.30, no.2, 2007 , pp. 205-209 More about this Journal
Abstract
A protein chip based on surface plasmon resonance (SPR) imaging was developed for measuring classical swine fever virus (CSFV) antibody using a recombinant gp55 protein as an antigen. The diagnostic potential of SPR imaging for detecting antibodies to the CSFV gp55 protein was compared with that of a enzyme -linked immunosorbent assay (ELISA) using 70 pig sera. There was a strong positive correlation between the SPR imaging and ELISA (n=70, r=0.916, p<0.01). Therefore, the SPR imaging, which is a label-free and high-through put method, is expected to be a valuable tool in the serodiagnosis of CSFV.
Keywords
Classical swine fever; Protein chip; Surface plasmon resonance imaging;
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1 Moennig V, Floegel-Niesmann G, Greiser- Wilke I. 2003. Clinical signs and epidemiology of classical swine fever: a review of new knowledge. Vet J 165(1) : 11-20   DOI   ScienceOn
2 Pringle CR. 1999. Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999. Arch Viral 144(10): 421-429   DOI   ScienceOn
3 Hulst MM, Westra DF, Wensvoort G, et al. 1993. Glycoprotein E1 of hog cholera virus expressed in insect cells protects swine from hog cholera. J Virol 67(9) : 5435-5442
4 Jung JM, Shin YB, Kim MG, et al. 2004. A fusion protein expression analysis using surface plasmon resonance imaging. Anal Biochem 330(2) : 251-256   DOI   ScienceOn
5 Lin M, Trottier E, Mallory M. 2005. Enzyme-linked immunosorbent assay based on a chimeric antigen bearing antigenic regions of structural proteins Ems and E2 for serodiagnosis of classical swine fever virus infection. Clin Diagn Lab Immunol 12(7): 877-881
6 Park BK, Park YH, Seo KS. 2000. Relation between lymphocyte subpopulations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex. J Vet Sci 1( 1) : 49-52
7 Rumenapf T, Stark R, Meyers G, et al. 1991. Structural proteins of hog cholera virus expressed by vaccinia virus: further characterization and induction of protective immunity. J Virol 65(2) : 589-597
8 Cho HS, Park SJ, Park NY. 2006. Serodiagnostic comparison of enzymelinked immunosorbent assay and surface plasmon resonance for the detection of antibody to porcine circovirus type 2. Can J Vet Res 70(4) : 263-268
9 Kim TJ, Cho HS, Park NY, et al. 2006. Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibody titres of Mycoplasma liyopneumoniae. J Vet Med B Infect Dis Vet Public Health 53(2) : 87-90   DOI   ScienceOn
10 Steiner G. 2004. Surface plasmon resonance imaging. Anal Bioanal Chem 379(3) : 328-331   DOI
11 Cho HS, Park NY. 2006. Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibodies of classical swine fever. J Vet Med Sci 68(12): 1327-1329   DOI   ScienceOn
12 Clavijo A, Lin M, Riva J. et al. 2001. Application of competitive enzyme-linked immunosorbentassay for the serologic diagnosis of classical swine fever virus infection. J Vet Diagn Invest 13(4): 357-360   DOI   ScienceOn