Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Development and evaluation of surface plasmon resonance imaging for the detection of antibodies against classical swine fever virus in swine

  • Cho, Ho-Seong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Tae-Uk (Jeoanam Livestock and Veterinary Science) ;
  • Park, Nam-Yong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University)
  • Published : 2007.06.30
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Abstract

A protein chip based on surface plasmon resonance (SPR) imaging was developed for measuring classical swine fever virus (CSFV) antibody using a recombinant gp55 protein as an antigen. The diagnostic potential of SPR imaging for detecting antibodies to the CSFV gp55 protein was compared with that of a enzyme -linked immunosorbent assay (ELISA) using 70 pig sera. There was a strong positive correlation between the SPR imaging and ELISA (n=70, r=0.916, p<0.01). Therefore, the SPR imaging, which is a label-free and high-through put method, is expected to be a valuable tool in the serodiagnosis of CSFV.

Keywords

References

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