• 한국환경생태학회지
• /
• 제32권1호
• /
• pp.1-22
• /
• 2018

본 연구의 목적은 백두대간 중 깃대배기봉부터 청화산까지 분포하는 관속식물상을 조사 및 분석하여 우리나라 산림생태계의 보전과 관리를 위한 기초자료 제공에 있으며, 연구결과를 요약하면 다음과 같다. 전체 조사구간의 식물상은 103과 379속 623종 4아종 121변종 23품종 등 771분류군이며, A구간은 377분류군, B구간은 395분류군, C구간은 278분류군, D구간은 325분류군, E구간은 534분류군, F구간은 406분류군이다. 희귀식물은 모데미풀, 도깨비부채, 꽃창포, 천마 등 32분류군이며, 멸종위기종(CR)은 1분류군, 위기종(EN)은 1분류군, 취약종(VU)은 11분류군, 약관심종(LC)은 18분류군, 자료부족종(DD)은 1분류군이다. 한국특산식물은 무늬족도리풀, 요강나물, 노랑갈퀴, 노각나무, 지리대사초, 두메꿩의밥 등 26분류군이다. 식물구계학적 특정식물은 총 143분류군이며, 이 중 V등급은 3분류군, IV등급은 12분류군, III등급은 41분류군, II등급은 42분류군, I등급은 45분류군이다. 귀화식물은 소리쟁이, 가죽나무, 붉은서나물, 개망초, 왕포아풀 등 41분류군이며, 생태계교란식물은 애기수영, 가시박, 돼지풀, 미국쑥부쟁이 등 4분류군이다. 기후변화 적응 대상식물은 43분류군이며, 특산식물계는 14분류군, 남방계 식물은 2분류군, 북방계 식물은 27분류군으로 나타났다.

• 한국환경생태학회지
• /
• 제25권4호
• /
• pp.447-469
• /
• 2011

문경 봉암사 일대의 식물상은 99과 303속 468종 1아종 70변종 13품종으로 총 552종류가 확인되었다. 식물상 중 IUCN 평가기준에 따른 희귀식물은 고란초, 너도바람꽃, 개회향, 구상난풀, 꼬리진달래, 참배암차즈기, 천마 등 12종류가 확인되었다. 특산식물은 무늬족도리, 진범, 점현호색, 청괴불나무, 벌개미취, 분취 등 11종류가 확인되었다. 식물구계학적 특정식물은 V등급에 고란초, 천마 등 2종류, IV등급에 승마, 등, 가는잎향유 등 5종류, III등급에 물박달나무, 당조팝나무, 청시닥나무, 산앵도나무, 개회나무 등 10종류, II등급에 돌양지꽃, 노랑제비꽃, 큰참나물, 왕쌀새, 박새 등 7종류, I등급에 거미고사리, 개비자나무, 박달나무, 대팻집나무, 피나무, 물고추나물, 숫잔대 등 24종류로 총 48종류(전체 552종류의 관속식물 중 약 8.7%에 해당)가 확인되었다. 귀화식물은 끈끈이대나물, 큰달맞이꽃, 컴프리, 붉은서나물 등 21종류가 확인되었으며, 귀화율은 552종류의 관속식물 중 3.8%로 분석되었다. 봉암사 일대에서 가장 중요한 식물군은 희귀식물인 고란초, 너도바람꽃, 천마, 수정난풀, 구상난풀, 꼬리진달래 및 중층 습원식물인 개회향, 꽃창포 등이 있으며, 외래종은 봉암사 경내의 식재종, 대상지안의 조림 및 인공림, 이입된 귀화식물 등으로 구분하였다.

• Journal of Plant Biotechnology
• /
• 제42권3호
• /
• pp.154-160
• /
• 2015

The plant breeding technology was developed with genetic engineering. Many researchers and breeders have turned from traditional breeding to molecular breeding. Genetically modified organisms (GMO) were developed via molecular breeding technology. Currently, molecular breeding technologies facilitate efficient plant breeding without introducing foreign genes, in virtue by of gene editing technology. Gene targeting (GT) via homologous recombination (HR) is one of the best gene editing methods available to modify specific DNA sequences in genomes. GT utilizes DNA repair pathways. Thus, DNA repair systems are controlled to enhance HR processing. Engineered sequence specific endonucleases were applied to improve GT efficiency. Engineered sequence specific endonucleases like the zinc finger nuclease (ZFN), TAL effector nuclease (TALEN), and CRISPR-Cas9 create DNA double-strand breaks (DSB) that can stimulate HR at a target site. RecQl4, Exo1 and Rad51 are effectors that enhance DSB repair via the HR pathway. This review focuses on recent developments in engineered sequence specific endonucleases and ways to improve the efficiency of GT via HR effectors in plants.

• 식물병연구
• /
• 제21권3호
• /
• pp.180-185
• /
• 2015

In the present work, a pair of primers specific to Tomato yellow leaf curl virus (TYLCV) was designed to allow specific amplification of DNA fragments from any TYLCV isolates using an extensive alignment of the complete genome sequences of TYLCV isolates deposited in the GenBank database. A pair of primers which allows the specific amplification of tomato ${\beta}$-tubulin gene was also analyzed as an internal PCR control. A duplex PCR method with the developed primer sets showed that TYLCV could be directly detected from the leaf crude sap of infected tomato plants. In addition, our developed duplex PCR method could determine PCR errors for TYLCV diagnosis, suggesting that this duplex PCR method with the primer sets is a good tool for specific and sensitive TYLCV diagnosis. The developed duplex PCR method was further verified from tomato samples collected from some farms in Korea, suggesting that this developed PCR method is a simple and reliable tool for rapid and large-scale TYLCV detections in tomato plants.

• Journal of Plant Biotechnology
• /
• 제44권1호
• /
• pp.1-11
• /
• 2017

Evolutionary studies conducted on NAC (NAM, ATAF1&2, and CUC2) genes for all major groups of land plants, indicate the presence of the NAC subfamilies, even in the early land plants. The varied roles played by NAC proteins in plant growth and development range from the formation of shoot apical meristem, floral organ development, reproduction, lateral shoot development, and defense responses to biotic and abiotic stresses. Considering the value and importance of solanaceous crops, the study of NAC proteins in these plants needs to be intensified. This will help to identify and functionally characterize their promoters, which will subsequently aid in engineering plants with improved performance under stressful conditions. In this review, the functionally characterized NAC transcription factors specific to tomato, potato, tobacco, chili pepper and eggplant (aubergine) are summarized, clearly indicating their biological functions in the defense mechanism of the plants, against biotic and abiotic stresses.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2010년도 정기총회 및 춘계학술발표회
• /
• pp.5-5
• /
• 2010

Selection of specific medicinal sources as well as bioactive compounds is important for the preparation of medicine and related products with good quality. It is necessary to pay close attention for choosing correct medicinal sources, particularly in case of medicinal plants, because of their diversity, which can affect the quality and efficacy of medicine. Discrimination of plants based on morphological or genetic characteristics has been used as a conventional classification method of pharmaceutical sources so far; however, more need demands more general methods for accurate quality assessment of medicinal plants. In this study, ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) technique applied to this metabolic profiling is a powerful tool due to its higher sensitivity, resolution, and speed compared to conventional HPLC technique. The metabolite profiling of several medicinal plants including Panax ginseng was carried out using UPLC/Q-TOF MS and total metabolites were then subsequently applied to various statistical tools to compare the patterns. The developed metabolomics tool with UPLC/Q-TOF MS successfully identified and classified the samples tested according to their origins.

• Plant Biotechnology Reports
• /
• 제4권1호
• /
• pp.95-99
• /
• 2010

Decamer RAPD primers were tested on dioeceious and hermaphrodite plants of Commiphora wightii to identify sex-specific molecular markers. Sixty different random decamer primers were screened out of which only three primers were found to be associated with sex expression. A ~1,280-bp fragment from the primer OPN06 was found to be present in all the female individuals. Another primer OPN 16 produced a unique ~400-bp amplification product in only hermaphrodite individuals. The third marker, OPA20 amplified a ~1,140-bp fragment from female and hermaphrodite DNAs, but failed to do so from the male plant DNAs.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제29권1호
• /
• pp.93-119
• /
• 2014

Tropical tasar silkworm, Antheraea mylitta (Lepidoptera: Saturniidae) is a polyphagous silk producing forest silkworm of commercial importance in India. Forest dependent people rear its larvae on different forestry host plants twice or thrice in a year for small household income. Larvae of A. mylitta feeds on many forest tree species, but always show a great degree of selectivity as a function of its behavioural responses to physical structure and chemical features of the host plants. Cocoon crop of A. mylitta is influenced by heterogeneity of tasar food plants and climatic conditions of the habitat. The role of host plants, temperature, humidity, rainfall, photoperiod and climatic variables on the growth and development of insects have clearly been demonstrated. This article entails an in-depth analysis on ecological and nutritional aspects of A. mylitta, which may provide selective information to researcher and forest managers, who are particularly associated with livelihood improvement of the poor people in forested area through location specific forest insect industry.

• 한국자원식물학회지
• /
• 제28권4호
• /
• pp.475-486
• /
• 2015

This survey was conducted in order to investigate the vascular plants of Hak Reservoir Valley in the DMZ Area, Korea. The vascular plants were collected six times (from April 2014 to November 2014) and consisted a total of 238 taxa; 69 families, 172 genera, 206 species, 2 subspecies, 26 varieties and 4 forms. The observed rare plant species as designated by Korea Forest Service were 3 taxa including Carex capricornis Meinsh. ex Maxim., Acorus calamus L., Penthorum chinense Pursh. Endemic plant species were 2 taxa including Salix koriyanagi Kimura, Clematis brachyura Maxim. 17 taxa were listed as specific plant species based on phytogeographical in the investigated area. 25 taxa were recorded as naturalized plants, and their Naturalization Ratio and Urbanization Index were recorded as 10.46%, and 7.78%, respectively. Of the 238 taxa listed; 105 were pasturing plants, 92 edible plants, 76 medicinal plants, 24 ornamental plants, 6 stain plants, 4 timber plants, 2 fiber plants, 2 industrial plants and 55 unknown plants.

• The Korean Journal of Ecology
• /
• 제18권1호
• /
• pp.43-54
• /
• 1995

Effects of environmental factors of N, P, pH, moisture, temperature and oxygen on growth and nitrogen fixation activity of kummerowia striate (Thunb.) Schindler seedling, bearing symbiotic root nodules, were quantitatively analyzed during the growing period. The specific nitrogenase activity (ARA) of nodules showed the maximum value of 187 μmol C₂H₄g fr wt-1 h-1 6 weeks after seeds were germinated. The total nitrogenase activities per plant attained as 1.56, 0.85, 0.09 and 4.0, 1.11, 0.04 μmol C₂H₄hr-1, respectively for the treatments of 1, 3 and 5 mM NO₃￣and NH₄＋ on the 60th day. While the plant grown in N-free media for 20 days after treatments of 5 mM NH₄+for 40 days resulted in 30 mg fr wt of nodule formation and exhibited the relative activities of 152％ and 162％ for total and specific ARA in comparison with those of control plant grown with N-free for 60 days. Total biomass and ARA was by 70％ and 86％ lower in N and P deficiency, respectively. The N and P deficient plot showed 70％ and 86％ decreases of total biomass and ARA in comparison with those of control. The plant grown with N-free for 20 days after pretreatment with N and P free media for 40 days showed the relative values of 77％, 118％ and 150％, respectively for nodule biomass, total and specific ARA in comparison with those of control. The treatment with acid or alkali gradients resulted in significant decreases of nodule biomass and ARA. The optimum temperature and pO₂for ARA were 30°C and 40 kPa, respectively. Two peaks of diurnal variation appeared at 11:00 and 23:00 o'clocks by the continuous light condition. The plants with water stress by temporary wilting point rsulted in 95~97％ inhibition for nodule respiration, transpiration and specific ARA. Transpiration and ARA ware recovered to 88％ and 38％ of those of water unstressed plants, respectively, 6 hours after the plants were rewatered from water stressed condition.