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http://dx.doi.org/10.1007/s11816-009-0113-8

Identification of RAPD markers linked to sex determination in guggal [Commiphora wightii (Arnott.)] Bhandari  

Samantaray, Sanghamitra (Directorate of Medicinal and Aromatic Plants Research (DMAPR))
Geetha, K.A. (Directorate of Medicinal and Aromatic Plants Research (DMAPR))
Hidayath, K.P. (Directorate of Medicinal and Aromatic Plants Research (DMAPR))
Maiti, Satyabrata (Directorate of Medicinal and Aromatic Plants Research (DMAPR))
Publication Information
Plant Biotechnology Reports / v.4, no.1, 2010 , pp. 95-99 More about this Journal
Abstract
Decamer RAPD primers were tested on dioeceious and hermaphrodite plants of Commiphora wightii to identify sex-specific molecular markers. Sixty different random decamer primers were screened out of which only three primers were found to be associated with sex expression. A ~1,280-bp fragment from the primer OPN06 was found to be present in all the female individuals. Another primer OPN 16 produced a unique ~400-bp amplification product in only hermaphrodite individuals. The third marker, OPA20 amplified a ~1,140-bp fragment from female and hermaphrodite DNAs, but failed to do so from the male plant DNAs.
Keywords
Commiphora wightii; Dioecious; Hermaphrodite; RAPD markers; Bulk segregant analysis;
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