• The Korean Journal of Mycology
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• v.32 no.2
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• pp.71-78
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• 2004

This study was conducted to investigate the diversity and occurrence frequency of ectomycorrhizal fruit bodies by planting sites from June 2000 to October 2001. A total of 3 classes 3 subclasses 8 orders 22 families 41 genera and 72 species (including two varieties) including saprophytic and ectomycorrhizal fungi was investigated. The mushrooms are classified into 9 families 21 genera and 48 species in Agaricales, 5 families 11 genera and 13 species in Aphllophorales, 3 families 3 genera and 4 species in Heterobasidiomycetes and 5 families 6 genus and 7 species in Gasteromycetdae. A total of 7 families 11 genera 30 species (2,451 ea.) of ectomycorrhizal mushroom was investigated. The occurrence frequency of mushrooms was 1,225, 179 and 130 times for Laccaria vinaceoavellanea, Amanita longistriata and Laccaria amethystea, respectively. The mushroom occurrence of ectomycorrhizal fungi was closely related to climatic conditions such as high air temperature, relative humidity and lots of rainfall from July to August. Diversity and distribution of ectomycorrhizal fungi by plots were very different because of variable local environments and different host plants in experimental plots. Laccaria vinaceoavellanea has showed very low host range of plant specificity because of mushroom occurrence in only Quercus sp. and Amanita longistriata, Russula bella and Inocybe sp. have showed wide host range of plant specificity because of mushroom occurrence in coniferous and broadleaved trees. The environment which has a favorable influence of mushroom occurrence was soil pH, organic matter and T/N ratio of soil enviromental and humidity of climatic environment.

• Development and Reproduction
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• v.15 no.2
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• pp.121-131
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• 2011

The aim of this study was to investigate the egg development and the color change of Amphiprion melanopus, by the live food and thyroid hormone. After fertilizing, it took 168 h until hatching. The size of hatching larvae were $3.5{\pm}0.3$ mm, and some individuals had small yolk sac. As reaching the particular age, they underwent the color change (metamorphosis) - Days after hatching (DAH) 10: black, DAH 15~20: the 3 white stripes appear, DAH 30: the dorsal & the caudal white stripe begin to disappear. DAH 90: body color become red and orange, adult: cinnamon body color and a white stripe on head. - The group fed enriched Artemia with Tigriopus japonicus grew the faster than with only Artemia. The fin coloration of the one fed the only T. japonicus has a vivid red and orange color. $T_3$-treated group (4 ppm) grew the faster than the others (0, 2, 6 ppm). The size of the 6 ppm group was the smallest. The results suggest T. japonicus can be used as a supplement with rotifer and Artemia for the juvenile development and coloration. $T_3$ is considered to be involved in the metamorphosis and color formation of Amphipirion sp.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.3
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• pp.63-79
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• 2020

The present study was to investigate the growth response and nutrient absorption in three willow species (Salix gracilistyla, S. koreensis and S. chaenomeloides) treated with nitrogen source (NH₄⁺:NO₃^- rate; 200:0, 150:50, 100:100, 50:150, 200:0) for a period of 90days. The height, dry weight and chlorophyll contents of three species of willows were found to be highest at 50:150 (NH₄⁺:NO₃^-) treatment. NO₃^--N was more effective than NH₄⁺-N in the early growth of three species of willows. The increase in percentage of NO₃^--N handling, T-N, NO₃^--N in plant tissue increased. The analysis of C, N and mineral content in various parts revealed that the amounts of C, N, K, Ca and Mg were higher in leaves than those in the stems and the roots. However, the amount of NO₃^--N and P were higher in roots than those in the leaves and the stems. Salix koreensis was excellent, followed by S. chaenomeloides and S. gracilistyla in absorption of nitrate nitrogen. Higher percentage of NO₃^--N, the amounts of T-N, NO₃^--N, P₂O₅, K, Na, Ca, and pH in soil were decreased.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.15 no.2
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• pp.191-202
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• 2011

Objectives: The questionnaires of body condition were developed to increase golf performance and to find psychological factors in junior golfer. This study was how much golf performances got improved after acupuncture treatment. Methods: October $28^{th}$, $29^{th}$ 2010 and January 2011, 130 junior golfers were taken 8 questionnaires. Sixty junior golfer of them were selected, twenty of them were taken acupuncture treatment spontaneously and forty of them were not taken it. The acupoints were K10, H3, Sp3, H7. The statistics was used paired student t-test of before and after treatment and correlates analysis for analyzing relationship with questionnaires. Results: 1. The result of reliability among questionnaires was signified. 2. About questionnaires, golf score was related with satisfied shots, to endure hungry and about digestion. (p<0.5) 3. Anxiety was related with pain in ribs, about sleep, about bowl movement and about digestion. 4. It was obtained Regression equation of 41.9 % prediction between anxiety and 4 questionnaires. 5. The result of paired T-Test, the difference was $2.45{\oplus}4.69$ between $1^{st}$ score and $2^{nd}$ score in acupuncture treated group. (p<0.05) Conclusions: This study was important to improve golf performance with Asian medicine. Further clinical research is necessary to develop more delicate analyses and questionnaires. Also through the diagnosis and corresponding treatments based on the Asian Medicine, the ways of improving golf performance should be developed.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.8
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• pp.1179-1187
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• 2001

In fish both humoral and cell mediated immune responses have been reported whereas antibodies recognizing specific cellular populations have not yet been developed except for ones recognizing surface Ig molecules on B lymphocytes. Our aim was to develop and characterize monoclonal antibodies (Mabs) specific for the immune-related cells. Mabs were produced by fusion of myeloma cells (SP2/0) with Balb/c mouse spleen cells previously sensitized against Israeli carp (I. carp) kidney mononuclear cells. We obtained 44 Mabs positively reacting with I. carp kidney mononuclear cells and partially characterized 7 Mabs in the morphological and mitogen-based proliferative aspects. Fluorescence-activated cell sorter (FACS) analysis against I. carp kidney cells by using 7 different Mabs showed 80.3% for ICK 17-4, 65.1% for ICK 2-3, 64.1% for ICK 25-1, 67.5% for lCK 22-1, 70.8% for ICK 16-2, 76.8% for ICK 13-2, 79.7% for ICK II-I. Panning method was used for the isolation of Mabs specific mononuclear carp spleen cells followed by Wright's stain. The stained cell populations were identified as monocytes (ICK 17-4, ICK 2-3, ICK 25-1, ICK 22-1 and ICK 16-2), lymphocytes (ICK 11-1), and a mixed cell population of monocytes and lymphocytes (ICK 13-2). In cell proliferation assay, monocytes purified by ICK 17-4, 2-3 and 22-1 efficiently responded to Con A and PHA, while ones separated by ICK 25-1 did not react with any mitogens. Lymphocytes isolated by ICK 11-1, though it is not known whether they are T or B cells, were more responsive to Con A than PHA or LPS, suggesting that fish immune cells are somewhat different from mammalian cells in responding to mammalian T or B cell mitogens.

• International journal of advanced smart convergence
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• v.11 no.4
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• pp.96-103
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• 2022

This paper presents the analysis of increasing the resolution of True-Time-Delay (TTD) by 0.5-bit for phased-array antenna system which is one of the Multiple-Input and Multiple Output (MIMO) technologies. For the analysis, a 5.5-bit True-Time Delay (TTD) integrated circuit is designed and analyzed in terms of beam steering performance. In order to increase the number of effective bits, the designed 5.5-bit TTD uses Single Pole Triple Throw (SP3T) and Double Pole Triple Throw (DP3T) switches, and this method can minimize the circuit area by inserting the minimum time delay of 0.5-bit. Furthermore, the circuit mostly maintains the performance of the circuit with the fully added bits. The idea of adding 0.5-bit is verified by analyzing the relation between the number of bits and array elements. The 5.5-bit TTD is designed using 0.18 ㎛ RF CMOS process and the estimated size of the designed circuit excluding the pad is 0.57×1.53 mm². In contrast to the conventional phase shifter which has distortion of scanning angle known as beam squint phenomenon, the proposed TTD circuit has constant time delays for all states across a wide frequency range of 4 - 20 GHz with minimized power consumption. The minimum time delay is designed to have 1.1 ps and 2.2 ps for the 0.5-bit option and the normal 1-bit option, respectively. A simulation for beam patterns where the 10 phased-array antenna is assumed at 10 GHz confirms that the 0.5-bit concept suppresses the pointing error and the relative power error by up to 1.5 degrees and 80 mW, respectively, compared to the conventional 5-bit TTD circuit.

• The Korean Journal of Pesticide Science
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• v.4 no.4
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• pp.35-39
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• 2000

In order to develop the indicators of environmental impact of pesticide, its actual usage in paddy rice was surveyed, and usage trends of individual pesticides were evaluated. The tendency of pesticide use indicated insecticide 43%, herbicide 29%, fungicide 27% and top ranking item in insecticide, herbicide and fungicide was carbofuran, molinate + pyrazosulfuran-ethyl, IBP. The usage statistics of formulation types showed GR>DP>WP>EC>FG>SP. Pesticide usage(a.i.) per hectare was 7.13kg and total usage for paddy rice was estimated at 8,387 M/T. In the result of comparison of fact-usage with pesticide consumption reported in 1998, the fitness was 94.7% for fungicide, 84.3% for insecticide, 77.8% for herbicide. The result of monitoring pesticide residue of unpolished-rice sampled from farm house of survey indicated 0.14 ppm(BPMC), 0.16 ppm(Isoprocarb), 0.17 ppm(Isoprothiolane). In case of rice straw, the residue level was 0.27 ppm(Isoprothiolane), 0.28 ppm(IBP), 0.39 ppm(Carbofuran). The residue levels of pesticides were below MRLs.

• Parasites, Hosts and Diseases
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• v.35 no.1
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• pp.55-62
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• 1997

The molecular weight 30 kDa membrane protein of Toxoplusma Sondii (Toxoplasma 30 kDa) apparently conserved in most strains of T. gondii and sera of infected hosts. The present study aimed to elucidate Toxoplasmc 30 kDa as a useful diagnotic antigen for serodiagnisis of toxoplasmosis by ELISA and for induction of protective immunity. Murine spleen cells immunized with the membrane antigen of T. gondii were fused with mouse Sp2/0-Ag 14 myeloma cells. Out of 8 clones selected, five were IgG2b, the others belonged to IgG 1 and IgG2a. The 30 kDa antigen was distributed mainly on the surface membrane of tachyzoites by indirect fluorescence method. Murine peritoneal macrophages which were activated by 30 kDa antigen produced more amounts of NO2 compared with crude antigen-treated group, however there were no significant differences in toxoplamacidal activity between the two groups. Higher specificity of Toxoplosma 30 kDa antigen was recognized for serodiagnosis of toxoplasmosis than the crude antigen. From these results, ToxopLasmo 30 kDa antigen enhances the cytotoxic effect of macrophages as well as a more reliable means for the serodiagnosis of toxoplasmosis by ELISA. Key words: Toxoplosma gondii, 30 kDa antigen (p30), mouse, serodiagnosis, macrophage, cytotoxicity.

• Applied Biological Chemistry
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• v.15 no.1
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• pp.27-33
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• 1972

The alkaline protease was isolated from the culture material of monascus sp. on wheat bran culture. The crude purification of this enzyme was extracted with distilled water and precipitated with ammonium sulfate of 0.5 saturation. And, the activity of this enzyme was determind very strongly by folin's colorimetric method. The optimal pH of this enzyme was ranging from pH 10 to 13 and the optimal temperature was $50^{\circ}C$. The pH stability was ranging from pH 5 to 12 and the enzyme activity was not inactivated by heat treatment in lower temperature than $40^{\circ}C$. The enzyme was protected from heat denature by the treatment of $Pb^#$, $Ba^#$, $Co^#$, $Zn^#$, and $Cu^#$, but was inactivated with $Hg^#$, $Fe^#$ strongly. Moreover, one of these metal ions, the cupper ion, has a strong protective activity on enzyme heat denature. And, it was not effected by treatment of EDTA.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.11
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• pp.1591-1598
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• 2004

To isolate the $\beta$-galactosidase producing thermophilic bacteria, samples of mud and water were collected from hot springs of avolcanic area near Golden Springs in New Zealand. Among eleven isolated strains, the strain of KNOUC112 produced the highest amounts of $\beta$-galactosidase at 40 h incubation time (0.013 unit). This strain was aerobic, asporogenic bacilli, immobile, gram negative, catalase positive, oxidase positive, and pigment producing. Optimum growth was at 70-72$^{\circ}C$, pH 7.0-7.2, and it could grow in the presence of 3% NaCl. The main fatty acids of cell components were iso-15:0 (30.26%), and iso-17:0 (31.31%). Based on morphological and biochemical properties and fatty acid composition, the strain could be identified as genus Thermus, and finally as Thermus thermophilus by phylogenetic analysis based on 16S rRNA sequence. So the strain is designated as Thermus thermophilus KNOUC112. A gene from Thermus thermophilus KNOUC112 encoding $\beta$-galactosidase was amplified by PCR using redundancy primers prepared based on the structure of $\beta$-galactosidase gene of Thermus sp. A4 and Thermus sp. strain T2, cloned and expressed in E. coli JM109 DE3. The gene of Thermus thermophilus KNOUC112 $\beta$-galactosidase(KNOUC112$\beta$-gal) consisted of a 1,938 bp open reading frame, encoding a protein of 73 kDa that was composed of 645 amino acids. KNOUC112$\beta$-gal was expressed as dimer and trimer in E. coli JM109 (DE3) via pET-5b.