Studies on the alkaline protease produced from Monascus sp.

Monascus 속(屬) 균주(菌株)가 생성(生成)하는 Alkaline Protease에 관(關)한 연구(硏究)

The alkaline protease was isolated from the culture material of monascus sp. on wheat bran culture. The crude purification of this enzyme was extracted with distilled water and precipitated with ammonium sulfate of 0.5 saturation. And, the activity of this enzyme was determind very strongly by folin's colorimetric method. The optimal pH of this enzyme was ranging from pH 10 to 13 and the optimal temperature was $50^{\circ}C$. The pH stability was ranging from pH 5 to 12 and the enzyme activity was not inactivated by heat treatment in lower temperature than $40^{\circ}C$. The enzyme was protected from heat denature by the treatment of $Pb^#$, $Ba^#$, $Co^#$, $Zn^#$, and $Cu^#$, but was inactivated with $Hg^#$, $Fe^#$ strongly. Moreover, one of these metal ions, the cupper ion, has a strong protective activity on enzyme heat denature. And, it was not effected by treatment of EDTA.

본(本) Monascus속(屬) 균주(菌株)를 Wheat bran 배지(培地)에 배양(培養)하여 여기서 얻은 Alkaline protease의 효소학적(酵素學的) 성질(性質)은 다음과 같다. 1) 본(本) Protease의 최적(最適) pH는 $10{\sim}12$에 위치(位置)하고 있으며 2) 최적온도(最適溫度)는 $50^{\circ}C$ 이었으며 3) 안정(安定) pH는 $5{\sim}12$에 위치(位置)하며 4) 본(本) Protease는 $40^{\circ}C$ 이하(以下)에서 안정(安定)하였으며 5) 금속(金屬) Ion 중(中) $Hg^#$와 $Fe^#$는 조해적(阻害的)으로 작용(作用)하여 $Pb^#$, $Ba^#$, $Co^#$, $Zn^#$, $Cu^#$등은 보호작용(保護作用)을 나타내었고 이 중(中) $Cu^#$는 $10^{-2}$ 농도(濃度)에서 본(本) protease의 실활(失活)을 강(强)하게 보호(保護)함을 알았다. 6) E.D.T.A는 본(本) Protease에 대(對)해서 아무 영향을 주지 않음을 알았다.