• Title/Summary/Keyword: SOX5

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miR-133a-3p and miR-145-5p co-promote goat hair follicle stem cell differentiation by regulating NANOG and SOX9 expression

  • Jian Wang;Xi Wu;Liuming Zhang;Qiang Wang;Xiaomei Sun;Dejun Ji;Yongjun Li
    • Animal Bioscience
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    • v.37 no.4
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    • pp.609-621
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    • 2024
  • Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR-145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145-5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.

Co-combustion of RPF in the Coal Power plant (석탄보일러에서 각종 RDF혼소시 다이옥신 농도 조사)

  • Choi, Yeon-Seok;Choi, Hang-Seok;Kim, Seock-Joon;Yoon, Kyoon-Duk
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.05a
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    • pp.452-455
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    • 2008
  • The co-combustion of coal and three kinds of RDFs(Refuse Plastic Fuel) mixture has been experimented in a commercially operating CFB coal boiler respectively and the pollutant emissions such as SOx, NOx, TSP and dioxine were measured at the stack. The mixing ratio with coal was 7.5% RPF, 7.5% RDF and 10% SDF respectively. During co-combustion, dioxine emission level was very low and SOx, NOx and TSP were decreased comparing the single coal combustion. Emitting dioxine concentration was proportioned to the chlorine content of RDF. These RPF, SDF and RDF could be determined to be a good alternative fuel of general coal.

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항만구역 내 선박 배기가스 산출량 연구 - 서산 대산항 컨테이너 부두를 중심으로 -

  • Kim, Hwa-Yeong
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2018.11a
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    • pp.357-357
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    • 2018
  • 국제해사기구(IMO)에서는 황산화물(SOx)에 대한 배출 규제를 강화하고 있다. 2020년 1월 1일부터는 황함유량 0.5% 이하 선박연료유 사용을 의무화하고 있다. 그리고 온실가스 배출량 모니터링을 2019년 1월 1월부터 시행하여 총톤수 5천톤 이상 선박은 연료유 사용량을 의무적으로 보고해야 한다. 또한 배출통제구역(Emission Control Area, ECA)이 확대되고 있으며 지역별로도 저유황유 사용 의무화를 도입하는 항만이 증가하고 있다. 이와 같이 항만구역에서 선박 배기가스 배출 규제를 강화하고 있다. 본 연구에서는 컨테이너 물동량이 증가하고 있는 서산 대산항 컨테이너 부두를 중심으로 항만구역에서 배출되는 배기가스를 산출하였다.

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Identification of a Gene for Aerobic Growth with a SoxS Binding Sequence in Escherichia coli by Operon Fusion Techniques

  • Lee, Yong-Chan;Kwon, Hyung-Bae;Lee, Sang-Ho;Kwon, Hye-Won;Sung, Ha-Chin;Kim, Joon;Choe, Mu-Hyeon
    • Journal of Microbiology and Biotechnology
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    • v.11 no.6
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    • pp.1115-1119
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    • 2001
  • Eight Escherichia coli cells with aerobic growth deflects were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created transcriptional fusion to lacZY. Two of these mutants, CLIO and CLl2, were irradiated with UV to obtain specialized transducing phages. The phages that took out the neighboring chromosomal DNA of the related gene responsible for deflective aerobic growth were identified. The in vivo cloned chromosomal sequence revealed that the mutated gene of CLIO was located at min 34.5 on the Escherichia coli linkage map and 1,599,515 on the physical map. The physical map indicated that there were 7 cistrons in the operon. We named this operon oxg10. The promoter sequence of oxg10 exhibited a possible binding site far SoxS, a transcriptional regulator that activates the transcription of various SoxRS regulon genes. Transferring the oxg10:: ${\lambda}placMu53$ mutation into the wild-type strain, RZ4500, resulted in the inhibition of normal aerobic growth, while the salute mutation in strain MO inhibited aerobic cell growth completely. The full operon sequences of oxg10 were cloned from the Excherichia coli genomic library. The mutated gene of CLl2 was identified to be a sucA gene encoding the ${\alpha}$-ketoglutarate dehydrogenase El component in the TCA cycle.

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The number of primitive endoderm cells in the inner cell mass is regulated by platelet-derived growth factor signaling in porcine preimplantation embryos

  • Jong-Nam Oh;Mingyun Lee;Gyung Cheol Choe;Dong-Kyung Lee;Kwang-Hwan Choi;Seung-Hun Kim;Jinsol Jeong;Chang-Kyu Lee
    • Animal Bioscience
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    • v.36 no.8
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    • pp.1180-1189
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    • 2023
  • Objective: Discovering the mechanism of cell specification is important to manipulate cellular lineages. To obtain lineage-specific cell lines, the target lineage needs to be promoted, and counterpart lineages should be suppressed. Embryos in the early blastocyst stage possess two different cell populations, the inner cell mass (ICM) and trophectoderm. Then, cells in the ICM segregate into epiblasts (Epi) and primitive endoderm (PrE). PrE cells in embryos show specific expression of platelet-derived growth factor (PDGF) and its receptor, PDGF receptor A (PDGFRA). In this study, we suppressed PDGF signaling using two methods (CRISPR/Cas9 injection and inhibitor treatment) to provide insight into the segregation of embryonic lineages. Methods: CRISPR/Cas9 RNAs were injected into parthenogenetically activated and in vitro fertilized embryos. The PDGF receptor inhibitor AG1296 was treated at 0, 5, 10, and 20 µM concentration. The developmental competence of the embryos and the number of cells expressing marker proteins (SOX2 for ICM and SOX17 for PrE) were measured after the treatments. The expression levels of the marker genes with the inhibitor were examined during embryo development. Results: Microinjection targeting the PDGF receptor (PDGFR) A reduced the number of SOX17-positive cell populations in a subset of day 7 blastocysts (n = 9/12). However, microinjection accompanied diminution of Epi cells in the blastocyst. The PDGF receptor inhibitor AG1296 (5 µM) suppressed SOX17-positive cells without reducing SOX2-positive cells in both parthenogenetic activated and in vitro fertilized embryos. Within the transcriptional target of PDGF signaling, the inhibitor significantly upregulated the Txnip gene in embryos. Conclusion: We identified that PDGF signaling is important to sustain the PrE population in porcine blastocysts. Additionally, treatment with inhibitors was a better method to suppress PrE cells than CRISPR/Cas9 microinjection of anti-PDGF receptor α gene, because microinjection suppressed number of Epi cells. The PDGF receptor might control the number of PrE cells by repressing the proapoptotic gene Txnip. Our results can help to isolate Epi-specific cell lines from blastocysts.

Assessment and Estimation of Particulate Matter Formation Potential and Respiratory Effects from Air Emission Matters in Industrial Sectors and Cities/Regions (국내 산업 및 시도별 대기오염물질 배출량자료를 이용한 미세먼지 형성 가능성 및 인체 호흡기 영향 평가추정)

  • Kim, Junbeum
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.4
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    • pp.220-228
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    • 2017
  • Since the fine particulate matters occurred from mainly combustion in industry and road transport effect to human respiratory health, the interest and importance are getting increased. In 2013, the World Health Organization (WHO) concluded that outdoor air pollution is carcinogenic to humans, with the particulate matter component ($PM_{10}$ and $PM_{2.5}$) of air pollution most closely associated with increased cancer incidence, especially cancer of the lung. Therefore, many researches have been studied in the quantification and data development of fine particulate matters. Currently, the Ministry of Environment and cities/regions are developing the fine particulate matter data and air emission information. Particularly just $PM_{10}$ and $PM_{2.5}$ data is used in the fine particulate matters warning and alert. The data of NOx, SOx, $NH_3$, which have the particulate matter formation potential are not well considered. Also, the researches related with particulate matter formation potential and respiratory effects by industrial sectors and cities/regions are not conducted well. Therefore, the purpose of this study is to evaluate and calculate particulate matter formation potential and respiratory effects in 11 industrial sectors and cities using NOx, SOx, $PM_{10}$, $NH_3$ data (developed by Ministry of Environment and National Institute of Environmental Research) in 2001 and 2013. The results of this study will be provided the particulate matter formation potential and respiratory effects and will be used for future the fine particulate matter researches.

A Study on Estimating Air Pullution in the Port of Incheon (인천항의 대기오염물질 배출량 산정 연구)

  • Lee, Jeong-Uk;Lee, Hyang-Suk
    • Journal of Korea Port Economic Association
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    • v.37 no.1
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    • pp.143-157
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    • 2021
  • International organizations such as the World Health Organization, the Organization for Economic Development and Cooperation, and major developed countries recognize the seriousness of air pollution. International organizations such as the International Maritime Organization have also implemented various regulations to reduce air pollution from ships. In line with this international trend, the government has also enacted a special law on improving air quality in port areas, and is making efforts to reduce air pollution caused by ports. The purpose of the Special Act is to implement comprehensive policies to improve air quality in port areas. This study sought to identify the emissions of each source of air pollutants originating from the port and prepare basic data on setting the policy priorities. To this end, the analysis was conducted in six categories: ships, vehicles, loading and unloading equipment, railways, unloading/wild ash dust, road ash dust, and the methodology presented by the European Environment Agency(EEA) and the United States Environmental Protection Agency(EPA). The pollutants subject to analysis were analyzed for carbon monoxide(CO), nitrogen oxides (NOX), sulfur oxides(SOX), total airborne materials(TSP), particulate matter(PM10, PM2.5), and ammonia(NH3). The analysis showed a total of 7,122 tons of emissions. By substance, NOX accounted for the largest portion of 5,084 tons, followed by CO (984 tons), SOX (530 tons), and TSP (335 tons). By source of emissions, ships accounted for the largest portion with 4,107 tons, followed by vehicles with 2,622 tons, showing high emissions. This proved to be the main cause of port air pollution, with 57.6% and 36.8% of total emissions, respectively, suggesting the need for countermeasures against these sources.

A Study on Estimating Ship Emission - Focusing on Gwangyang Port and Ulsan Port (선박에 기인한 대기오염물질 배출량 산정 연구 -광양항과 울산항을 중심으로)

  • Zhao, Ting-Ting;Yun, Kyong-Jun;Lee, Hyang-Sook
    • Journal of Korea Port Economic Association
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    • v.35 no.2
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    • pp.93-108
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    • 2019
  • Recently, air pollution from the marine ports has become a serious issue all over the world. Because marine trade accounts for 99.7% of Korea's trade, efforts are required to recognize the level of port pollution and establish environmental policies. This study estimates air pollution emitted during the berthing process in the Gwangyang and Ulsan ports. Data on ship activity and characteristics are collected and reasonable methodologies and factors from EEA and EPA are adopted. The results show that 253.09 tons of CO, 1986.61 tons of NOx, 684.01 tons of SOx, 47.88 tons of $PM_{10}$, and 44.69 tons of $PM_{2.5}$ are emitted at the Gwangyang port. Further, the Ulsan port emitted 212.28 tons of CO, 1712.54 tons of NOx, 573.72 tons of SOx, 40.16 tons of $PM_{10}$, and 37.48 tons of $PM_{2.5}$. A stage-by-stage plan for installing AMP infrastructure is suggested as part of a green port policy. This research provides the current pollution status and contributes guidelines for the direction of future policy.

An Evaluation on the Combustion Characteristics of Heavy Oil-Water Emulsions (중질유-물 유화연료의 연소특성 평가)

  • Lee, Yong-Il
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.26 no.12
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    • pp.1722-1728
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    • 2002
  • Most researches regarding emulsified fuel were in the areas of emulsifier, emulsified fuel manufacturing and emulsified fuel droplet combustion, but there were little papers published regarding emulsified fuel combustion and boiler efficiency in an industrial boiler. The main purpose of this study is to clarify whether improvements in the boiler efficiency and the reduction of pollutants such as CO, NOx, SOx and smoke exist or not when emulsified fuels are combusted in the commercial boiler. Main experimental parameters were water content in heavy oil , excess $O_2$, and boiler load. The fuels used in this experiment were 0.5 B-C, and 5 kinds of 0.5 B-C/water emulsified fuels. The combustion characteristics of heavy oil and its emulsions with water were investigated in an industrial boiler. The combustion stability was monitored and exhaust gases such as CO, NOx, SOx and smoke were measured with excess $O_2$ and combustion load. In case of emulsified fuel combustion, flame stability was poor and boiler efficiency was lowered by 1.6~5.7%, but emission levels of CO and smoke were improved.

Subpopulations of miniature pig mesenchymal stromal cells with different differentiation potentials differ in the expression of octamer-binding transcription factor 4 and sex determining region Y-box 2

  • Jeon, Ryounghoon;Park, Sungjo;Lee, Sung-Lim;Rho, Gyu-Jin
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.3
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    • pp.515-524
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    • 2020
  • Objective: Human mesenchymal stromal cells (MSCs) exhibit variable differentiation potential and can be divided accordingly into distinct subpopulations whose ratios vary with donor age. However, it is unknown whether the same is true in pigs. This study investigated MSC subpopulations in miniature pig and compared their characteristics in young (2 to 3 months) and adult (27 to 35 months) pigs. Methods: Osteogenic, chondrogenic, and adipogenic capacity of isolated MSCs was evaluated by von Kossa, Alcian blue, and oil red O staining, respectively. Cell surface antigen expression was determined by flow cytometry. Proliferative capacity was assessed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Expression of marker genes was detected by quantitative real-time polymerase chain reaction. Results: Porcine MSCs comprised cells with trilineage and bilineage differentiation potential (tMSCs and bMSCs, respectively) and non-differentiating stromal cells (NDSCs). The tMSC and bMSC fractions were smaller in adult than in young pigs (63.0% vs 71.2% and 11.6% vs 24.0%, respectively, p<0.05); NDSCs showed the opposite trend (25.4% vs 4.8%; p<0.05). Subpopulations showed no differences in morphology, cell surface antigen expression, or proliferative capacity, but octamer-binding transcription factor 4 (OCT4) expression was higher in tMSCs than in bMSCs and NDSCs (p<0.05), whereas sex determining region Y-box 2 (SOX2) expression was higher in tMSCs and bMSCs than in NDSCs (p<0.05). Aging had no effect on these trends. Conclusion: Porcine MSCs comprise distinct subpopulations that differ in their differentiation potential and OCT4 and SOX2 expression. Aging does not affect the characteristics of each subpopulation but alters their ratios.