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Introduction of Denitrification Method for Nitrogen and Oxygen Stable Isotopes (δ15N-NO3 and δ18O-NO3) in Nitrate and Case Study for Tracing Nitrogen Source (탈질미생물을 이용한 질산성 질소의 산소 및 질소 동위원소 분석법 소개)

  • Lim, Bo-La;Kim, Min-Seob;Yoon, Suk-Hee;Park, Jaeseon;Park, Hyunwoo;Chung, Hyen-Mi;Choi, Jong-Woo
    • Korean Journal of Ecology and Environment
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    • v.50 no.4
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    • pp.459-469
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    • 2017
  • Nitrogen (N) loading from domestic, agricultural and industrial sources can lead to excessive growth of macrophytes or phytoplankton in aquatic environment. Many studies have used stable isotope ratios to identify anthropogenic nitrogen in aquatic systems as a useful method for studying nitrogen cycle. In this study to evaluate the precision and accuracy of denitrification bacteria method (Pseudomonas chlororaphis ssp. Aureofaciens ($ATCC^{(R)}$ 13985)), three reference (IAEA-NO-3 (Potassium nitrate $KNO_3$), USGS34 (Potassium nitrate $KNO_3$), USGS35 (Sodium nitrate $KNO_3$)) were analyzed 5 times repeatedly. Measured the ${\delta}^{15}N-NO_3$ and ${\delta}^{18}O-NO_3$ values of IAEA-NO-3, USGS 34 and USGS35 were ${\delta}^{15}N:4.7{\pm}0.1$${\delta}^{18}O:25.6{\pm}0.5$‰, ${\delta}^{15}N:-1.8{\pm}0.1$${\delta}^{18}O:-27.8{\pm}0.4$‰, and ${\delta}^{15}N:2.7{\pm}0.2$${\delta}^{18}O:57.5{\pm}0.7$‰, respectively, which are within recommended values of analytical uncertainties. Also, we investigated isotope values of potential nitrogen source (soil, synthetic fertilizer and organic-animal manures) and temporal patterns of ${\delta}^{15}N-NO_3$ and ${\delta}^{18}O-NO_3$ values in river samples during from May to December. ${\delta}^{15}N-NO_3$ and ${\delta}^{18}O-NO_3$ values are enriched in December suggesting that organic-animal manures should be one of the main N sources in those areas. The current study clarifies the reliability of denitrification bacteria method and the usefulness of stable isotopic techniques to trace the anthropogenic nitrogen source in freshwater ecosystem.

Present Status and Future Prospect of Satellite Image Uses in Water Resources Area (수자원분야의 위성영상 활용 현황과 전망)

  • Kim, Seongjoon;Lee, Yonggwan
    • Korean Journal of Ecology and Environment
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    • v.51 no.1
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    • pp.105-123
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    • 2018
  • Currently, satellite images act as essential and important data in water resources, environment, and ecology as well as information of geographic information system. In this paper, we will investigate basic characteristics of satellite images, especially application examples in water resources. In recent years, researches on spatial and temporal characteristics of large-scale regions utilizing the advantages of satellite imagery have been actively conducted for fundamental hydrological components such as evapotranspiration, soil moisture and natural disasters such as drought, flood, and heavy snow. Furthermore, it is possible to analyze temporal and spatial characteristics such as vegetation characteristics, plant production, net primary production, turbidity of water bodies, chlorophyll concentration, and water quality by using various image information utilizing various sensor information of satellites. Korea is planning to launch a satellite for water resources and environment in the near future, so various researches are expected to be activated on this field.

Effect of Carbon Dioxide Concentration, Temperature, and Relative Drought on Growth Responses and Yield in Spring Potato (Solanum tuberosum L.) (이산화탄소와 온도 그리고 한발 영향에 따른 감자의 생육과 수량반응)

  • Lee, Yun-Ho;Cho, Hyeoun-Suk;Kim, Jun-Hwan;Sang, Wan-Gyu;Shin, Pyong;Baek, Jae-Kyeong;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.2
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    • pp.149-158
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    • 2018
  • Agriculture is strongly influenced by climate change such as increased temperature and carbon dioxide ($CO_2$). This study describes the effects of climate change elevated $CO_2$, temperature, and relative drought on growth responses and yield in potato (Solanum tuberosum L.). The assessment was conducted for spring seasons in Soil-Plant-Atmosphere Research (SPAR) chamber at National Institute of Crop Science (NICS). Potatoes exhibit a positive response to $CO_2$ enrichment but water stress primarily reduces potato canopy and tuber yield. Elevated $CO_2$ and temperature increased both dry weight and tuber yield. Elevated $CO_2$ and temperature influenced SPAR 2 plants to a larger, and tuber increased yield up to 28% of than in SPAR 1(30-year average temperature at 450 ppm of $CO_2$). Our study findings indicate that tuber yield increase in potato under high $CO_2$ concentration was due to an increase in the size of individual tubers rather than in the number of the tubers per plant. On other hand, SPAR 3(30-year average temperature $+2.8^{\circ}C$ at 700 ppm of $CO_2$ under water stress) was lower than SPAR 2(30-year average temperature $+2.8^{\circ}C$ at 700 ppm of $CO_2$) nearly 56% of tuber yield due to drought. The results confirm potato drought sensitivity in terms of yield response. The experiment also showed that, in the conditions of climate change, climate change scenarios that improve cropping systems with potato.

Growth and Yield Related Characteristics of Soybeans for the Estimation of Grain Yield in Upland and Drained-Paddy Field (콩 논.밭 재배에서 수랑예측을 위한 생육과 수량 관련 형질의 비교)

  • Cho, Young-Son;Park, Ho-Gi;Kim, Wook-Han;Kim, Sok-Dong;Seo, Jong-Ho;Shin, Jin-Chul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.7
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    • pp.599-607
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    • 2006
  • The experiments were carried out to develop simulation model for estimating the yield of soybean in upland and paddy field condition. Field experiments were done at National Institute of Crop Science in 2005. The evaluated soybean cultivars were Taekwangkong, Daewonkong, and Hwangkeumkong. Soybean seeds were planted by hill seeding with 3-4 seeds and row and hill spacing were $60{\times}10cm$ in upland and $60{\times}15cm$ in paddy field. Seeds were sown on row (without making ridge) and on the top of ridge in upland and paddy field, respectively. Field parameters were measured yield components ($plants/m^{2}$, pod no./plant, and 100-seed weight, seed yield and growth characteristics (stem length, leaf area at each stage, and dry weight of shoot) and after measuring they were compared the relationships with seed yield and yield components and seed yield and growth characteristics. Seed yield of soybean was affected by cultivars and planting density. Seed yield was higher in upland than paddy field due to the higher planting density in upland field. The upland soybeans generally had lower 100-seed weight than that of paddy field. Seed yield of soybean in a paddy field was greatest in Taekwangkong and followed by Daewonkong and Hwangkeumkong. The harvest index of taekwangkong and Hwanggumkong was higher in upland than paddy field, however, it was higher in paddy field than upland in Daewonkong. Seed yield was greatest in Daewonkong in both experimental fields. The greatest stem length was observed in taekwangkong and Hwanggumkong (R6) in late growth stage in paddy field. Dry weight of shoot and pod, pod number, stem length, and stem diameter were higher grown in paddy field than grown in upland. Crop growth rate (CGR) of cultivars was higher in paddy field after 8 WAS(weeks after sowing) and it was greatest at 13 WAS in Daewonkong among the cultivars. In upland field, CGR was greatest in Taekwangkong and then followed by Daewonkong and Hwanggumkong during 12 and 15 WAS. There was no significant relationships between 100-seed weight and seed yield in both experimental fields. A significant positive relationship was observed between seed number and seed yield. The correlation coefficients between leaf area and shoot dry weight were about 0.8 during the whole growth stage except 5 WAS and 4-5 WAS in paddy field and upland, respectively. This experiment was done just one year and drained paddy field condition was not satisfied drained condition successfully at 7th leaf age of soybean by the heavy rain, so we suggest that the excessive soil water reduced seed yield in paddy field and the weather condition should be considered for utilizing of these results.

Assessment of Hydrochemistry and Irrigation Water Quality of Wicheon Watershed in the Gyeongsangbuk-do (경상북도 위천수계의 수리화학적 특성 및 관개용수 수질평가)

  • Lee, Gi-Chang;Park, Moung-Sub;Kim, Jae-Sik;Jang, Tae-Kwon;Kim, Hyo-Sun;Lee, Hwa-Sung;Son, Jin-Chang
    • Korean Journal of Environmental Agriculture
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    • v.39 no.1
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    • pp.36-43
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    • 2020
  • BACKGROUND: Wicheon watershed has the largest irrigation area among the mid-watershed of Nakdong river. However, no investigation of irrigation water quality has been conducted on the Wicheon watershed, which evaluates the effects on the soil quality and crop cultivation. Therefore, this study aims to provide various assessments of water quality of Wicheon watershed as the scientific basic data for efficient agricultural activities. METHODS AND RESULTS: Water sampling was performed in five locations of the first tributaries of Wicheon. Wicheon watershed showed clean water quality with very low organic matters and safe water quality from metals at all points of investigation. It was estimated that the natural chemical components of Wicheon watershed were originated from water-rock interaction in Gibbs diagram. All samples were concentrated in the type of Ca-HCO3-Cl in the Piper diagram. The quality of irrigation water was evaluated with sodium adsorption ratio (SAR), residual sodium carbonate (RSC), permeability index (PI), and percent sodium (%Na). The values of these water quality indices were in the range of 0.37-0.67, -2.11--0.24, 41.13-84.52% and 11.28-21.84%, respectively, and were classified as good grades at all sites. CONCLUSION: The water quality of Wicheon watershed was very low in salt, indicating good irrigation water suitable for growing agricultural products. We hope that the results of this study will be used as the basic data for the cultivation of agricultural products and promotion of their excellence.

Responses of Resistant Genes to Barley Yellow Mosaic Virus (BaYMV) Strains in Korea (국내 분포 보리호위축바이러스(Barley Yellow Mosaic Virus) strain에 대한 저항성 유전자 반응)

  • Park, Jong-Chul;Noh, Tae-Hwan;Park, Chul-Soo;Kang, Chun-Sik;Kang, Mi-Hyung;Lee, Eun-Sook;Lee, Jun-Hee;Lee, Jung-Joon;Kim, Tae-Soo
    • Research in Plant Disease
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    • v.15 no.2
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    • pp.72-76
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    • 2009
  • It was investigated the responses of BaYMV resistant genes to Korean BaYMV(Barley yellow mosaic virus) strains. BaYMV was distributed dominantly with about 51% detection ratio among the three investigated virus such as BaYMV, BaMMV(Barley mild mosaic virus) and SBWMV(Soil-borne wheat mosaic virus) in ELISA test. Double infection with BaYMV and BaMMV was detected also higher as 38.8%, however, BaMMV sole infection ratio was lower with only 1.4%. The 11 BaYMV resistant genes were tested their responses to four Korean BaYMV strains, BaYMV-N, H, I and M. Generally, rym 3 genes showed resistant to Korean BaYMV strains and rym 4m and 5a also was better. Three genes, rym 1+5(Mokusekko-3), rym 3(Ea 52, Baitori) and rym 5a(Solan) showed resistant responses to BaYMV-N type. In -H strain test, seven genes that rym 2(Mihori Hadaka 3), rym 3(Ea 52, Haganemugi, Baitori), rym 4m(Diana, Franka), rym 5a(Solan), rym 7(Hor 3365), rym 9(Bulgarian 347), rym 12(Jochiwon Covered 2) were considered as resistant. The three genes that rym 1+5, rym 3 and rym 5a was effective to -I strain, and rym 3, rym 4m and rym 5a showed resistant to -M strain.

Influences of the Plant Growth under Beta-Rays Irradiation at Low Dose (저 선량 베타선의 조사에 의한 식물의 생장에 미치는 영향)

  • Lee, Byung-Koo;Im, In-Chul;Kim, Jong-Eon
    • Journal of radiological science and technology
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    • v.33 no.2
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    • pp.143-148
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    • 2010
  • This study is to analyze effects of the growth of Chunhyang Young Radish (CYR) and Altari Radish (AR) according to the exposure for 31 days at low dose ${\beta}$-rays. This test has one contrast sample and eleven test samples each as to AR and CYR. The seeds from contrast and test sample were planted in the culture soil after 8 seeds were chosen from each with identical condition. The accumulated dose of test samples has been measured at consistent time on a daily basis for 31 days. The growing process and germination have been measured twice at consistent time in each week. The number of leaves, length of first leave and weight have been acquired average value by measuring for 20 and 25 days, respectively after being planted. The result of test sample in case of 25 days shows that 5% increase in length and 36% increase in weight for AR each at accumulated dose 0.01 Gy compared to the contrast sample. And the length of CYR has increased by 13~17% and 1% at accumulated dose 0.01~0.08 Gy and 0.3 Gy compared to the contrast sample. For the weight at accumulated dose 0.05 Gy and 0.23 Gy has increased by 36% and 2% compared to contrast sample. As to the number of leaves, AR has increased by 0~50% at accumulated dose 0.01-0.32 Gy compared to contrast sample. It also shows that the CYR has increased to 0~67% at accumulated dose 0.01-0.62 Gy compared to contrast sample. As a result of this study, it indicates that both AR and CYR has generally increased in their length, weight, and the number of leaves at low level accumulated dose part 0.01~0.2 Gy. The size of cell, area of nucleus and density of cell for test sample has been observed quite similar to the ones from contrast sample through microscope. In conclusion, AR and CYR irradiated by ${\beta}$-rays have estimated that they are achieved a rapid growth at low level accumulated dose region corresponding to its radiation hormesis theory. Further studies need to confirm the correlation between the radiation hormesis and the growth of the plants.

Germination of Two Rice Cultivars and Several Weed Species (벼와 수종(數種) 논잡초(雜草)의 휴면성(休眠性)과 발아성(發芽性))

  • Kim, Soon-Chul;Moody, Keith
    • Korean Journal of Weed Science
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    • v.9 no.2
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    • pp.116-122
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    • 1989
  • An experiment was carried out at the International Rice Research Institute in 1987 to understand the seed dormancy and germination habit of rice and several weed species. The germinability of the weed seeds just after harvest was variable depending on the species and ranged from 0 to 72%. Two rice cultivars, IR64(lowland type) and UPLRi-5(upland type) had higher than 95% in germination ability throughout the experimental period due to the fact that the rice seeds came from the harvest of the previous season and dormancy had already been overcome. The length of the storage period needed to overcome dormancy at room temperature($25{\pm}2^{\circ}C$) was about 50 days for Echinochloa glabrescens Munro ex Hook, f., more than 60 days for E. crus-galli ssp. hispidula (Retz.) Honda and 20 days for Ludwigia octovalvis(Jacq.) Raven. Seeds of E. colona(L.) Link, Monochoria vaginalis(Burm. f.) Presl, Fimbristylis miliacea(L.) Vahl and Cyperus difformis L. appeared to have no dormancy. Among the nine species M. vaginalis had the lowest germination of less than 1% throughout the experimental period. However, its seed germinated easily when planted in soil. The low germinability of E. glabrescens, E. crus-galli ssp. hispidula and L. octovalvis just after harvest could be overcome through pretreatment of seeds either by soaking in nitric acid(0.1N) for 1 day or removal of the hull in the grass species, the nitric acid treatment being superior. The results imply that germination habit of weed species varied depending on the species through their differential dormancy period or differential germination strategy.

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Status of Paddy Weed Flora and Community Dynamics in Korea (한국(韓國)의 논잡초분포(雜草分布) 및 군락현황(群落現況))

  • Kim, Soon-Chul
    • Korean Journal of Weed Science
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    • v.3 no.2
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    • pp.223-245
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    • 1983
  • Nationwide weed survey of paddy rice field conducted in 1981 and 1971 was compared and determined major dominant weed community types distributed by province. Based on the similarity coefficients between 1971 and 1981 the floristic composition based on the degree of dominance was greatly dissimilar while kind of weed flora were not much differed. Degree of dominance were concentrated to perennial weeds in 1981 while these were at animal weeds in 1971. The moat important 10 weed species and their dominance in 1981 were Monochoria vaginalis Presl.(22.2%), Sagittaria pygmaea Miquel(17.5%), S. trifolia L.(9.0%), Poramogeton distinctus Benn.(9.0%), Cyperus serotinus Rottb.(8.5%), Rotala indica Koehne(6.0%), Aneilema japonica Kunth(4.4%), Lindernia procumbens Philcox(3.9%), Eleocharis kuroguwai Ohwi(3.4%) and Ludwigia proarrara Roxb(3.0%), respectively while these for 1971 were R. indica(34.5%), Eleocharia acicularis Roem, et Schult (11.9%), M. vaginalis(11.1%), Cyperua difformis L.(8.7%), Echinochloa crus-galli Beauv.(6.9%), L. procumbens(3.3%), P. distinctus(3.1%), A. Japonica (2.4%), E. kuroguwai(1.8%) and P. hydropiper(1.8%), respectively. Weed occurrence was also closely related with soil type, cropping pattern and cultural practices. Particularly, the occurrence of P. distincrus was negatively correlated with the degree of land utilization. Weed community types dafined by two-dimensional ordination analysis were 11 for Jeonnam province, 9 for Gyeoaggi, Gangweon, Chungbuk and Gyeongnam provinces, 8 for Jeonbuk and Gyeongbuk provinces, 7 for Chungnam province and 4 for Jeju province, respectively.

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Characterization of Weed Occurrence in Major Horticultural Crops - III. Phenological Aspects of Major Weeds (원예경작지(園藝耕作地)에서의 잡초발생(雜草發生) 특성에 관(關)한 연구(硏究) - III. 주요잡초종(主要雜草種)의 발생계절성(發生季節性))

  • Woo, I.S.;Pyon, J.Y.;Guh, J.O.
    • Korean Journal of Weed Science
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    • v.9 no.2
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    • pp.130-140
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    • 1989
  • 1) Dormancy brocken weed seeds were planted in soil at 15 days interval from June to December in 1986 and 1987 and test of normality and normal distribution curve were made to determine seasonal distribution characteristics of weed emergence in fields. Monthly emergence distribution pattern of each species can be concluded as following normal distribution equations. E. crusgalli $y={\frac{1}{2.52{\sqrt{2{\pi}}}}}e^{-{\frac{(x-10.057)^2}{12.7}}}$ E. indica $y={\frac{1}{2.17{\sqrt{2{\pi}}}}}e^{-{\frac{(x-9.16)^2}{9.45}}}$ A. lividus $y={\frac{1}{7.74{\sqrt{2{\pi}}}}}e^{-{\frac{(x-10.06)^2}{15.46}}}$ S. nigrum $y={\frac{1}{2.7{\sqrt{2{\pi}}}}}e^{-{\frac{(x-9.695)^2}{14.58}}}$ C. busrsa-pastoris $y={\frac{1}{2.83{\sqrt{2{\pi}}}}}e^{-{\frac{(x-9.02)^2}{16.02}}}$ D. sanguinalis $y={\frac{1}{2.8{\sqrt{2{\pi}}}}}e^{-{\frac{(x-8.58)^2}{15.67}}}$ S. viridis $y={\frac{1}{2.72{\sqrt{2{\pi}}}}}e^{-{\frac{(x-9.36)^2}{14.8}}}$ C. album $y={\frac{1}{2.596{\sqrt{2{\pi}}}}}e^{-{\frac{(x-8.07)^2}{13.48}}}$ P. oleraeda $y={\frac{1}{2.45{\sqrt{2{\pi}}}}}e^{-{\frac{(x-10.83)^2}{12.01}}}$ 2) Emergence peak period of weed species tested were from the end of May to early August and yearly variation of emergence was observed in E. crus-galli, S. viridis, S, nigrum, and P. oleracea and this fact may more related to rainfall pattern rather than temperature.

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