• Title/Summary/Keyword: SH2B1

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Isolation and Characterization of Plant Growth Promoting Bacteria Pseudomonas sp. SH-26 from Peat Soil (이탄 토양으로부터 식물생육촉진세균 Pseudomonas sp. SH-26의 분리 및 특성)

  • Ho-Young Shin;Da-Son Kim;Chang-Ho Lee;Dong-Soek Lee;Song-Ih Han
    • Journal of the Korean Applied Science and Technology
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    • v.41 no.2
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    • pp.199-207
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    • 2024
  • We conducted to investigate both plant growth-promoting and plant disease-controlling activities of bacterial strains isolated from soil. Among the 48 isolated strains, SH-23, SH-26, SH-29, and SH-33 were identified as excellent strains for the production of β-glucosidase, cellulase, amylase, and protease. These 4 strains exhibited antifungal activity against plant pathogenic fungi (Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Colletotrichum acutatum). Strain SH-26, which exhibited excellent organic matter decomposition and antifungal activity against plant pathogenic fungi, was selected as the final superior strain. Upon determining the 16S rRNA gene sequence of the selected SH-26 strain, it exhibited 100% similarity with Pseudomonas knackmussii HG322950 B13T, Pseudomonas citronellolis BCZY01000096 NBRC 103043T, and Pseudomonas delhiensis jgi.1118306 RLD-1T. Furthermore, it was confirmed that the Pseudomonas sp. SH-26 exhibited siderophore production, nitrogen fixation ability, and the production of Indole-3-acetic acid.

Effect of Embryo Maturity and Medium on Callus Formation and Plant Regeneration from Immature Embryo of Eleutherococcus senticosus (가시오갈피 미숙배로부터 Callus 형성 및 식물체 재분화에 미치는 배의 성숙정도 및 배지의 효과)

  • 유창연
    • Korean Journal of Plant Resources
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    • v.10 no.2
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    • pp.122-127
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    • 1997
  • This study was conducted to establish mass propagation system from the tissue culture using immature embroys in Eleutherococus senticosus. Immature embroys from seeds were removed under the microscope and placed on modified SH and WPM medium containing several plant growth regulators. The calli were well formed on media containing 1mg/l of 2,4-D on modified SH medium and 1mg/l of 2,4-D and 3mg/l of TDZ on WPM medium. Shoot regeneration was better on modified SH or WPM medium with combination of high concentration of TDZ and low concentration of 2,4-D. Treatment of 2,4-D alone was better than treatment of TDZ alone in callus induction on modified SH medium but plant regeneration reversed. Treatment of 2.4-D and TDZ combination was better than treatment of 2,4-D alone in callus induction on WPM medium. The results of callus formation and shoot regeneration on WPM media differed to those of SH media. The rate of callus formation was nearly 83% when 2,4-D was added to SH medium on concentration of 1mg/l. The rate of callus formation was nearly 38% when combination treatment of 2,4-D 1mg/l and TDZ 3mg/l was added to WPM medium. Also, plant regeneration differed depending on the mature degree of immature embryo.

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Anti-Inflammatory Activity of Ethanol Extracts from Hizikia fusiformis Fermented with Lactic Acid Bacteria in LPS-Stimulated RAW264.7 Macrophages (유산균 종류에 따른 발효톳 추출물의 항염증 활성)

  • Kwon, Myeong Sook;Mun, Ok-Ju;Bae, Min Joo;Lee, Seul-Gi;Kim, Mihyang;Lee, Sang-Hyeon;Yu, Ki Hwan;Kim, Yuck Yong;Kong, Chang-Suk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.10
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    • pp.1450-1457
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    • 2015
  • The anti-inflammatory effect of ethanol extracts from Hizikia fusiformis fermented with and without lactic acid bacteria was compared in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. The fermentation was done using Weissella sp. SH-1 and Lactobacillus casei in a mixture of glucose and lactate source at $30^{\circ}C$ for 30 days. As a result, we confirmed that the fermentation of H. fusiformis with lactic acid bacteria inhibited LPS-stimulated nitric oxide (NO) production and the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, interleukin (IL)-6, tumor necrosis factor ${\alpha}$, and IL-$1{\beta}$ as important inflammatory factors. During a comparison analysis, we found that L. casei fermented groups significantly suppressed NO production by regulating iNOS and COX-2 expression. Also, the effective suppression of pro-inflammatory cytokine and LPS-induced activation of mitogen- activated protein kinase indicated that the fermentation using Weissella sp. SH-1 and L. casei may provide an increment towards the extraction of active components, which are effective anti-inflammatory agents.

Performance Investigation of Solar-Heating Ocean Thermal Energy Conversion (SH-OTEC) in Korea (태양열 이용 해양온도차발전시스템의 성능 예측)

  • Nguyen, Van Hap;Lee, Geun Sik
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.1
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    • pp.43-49
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    • 2013
  • The use of ocean thermal energy conversion (OTEC) to generate electricity is one of the methods proposed to utilize renewable energy and to protect the environment. In this study, simulations were performed to investigate the effect of weather conditions in the Ulsan region, Korea, on the efficiency of a solar-heating OTEC (SH-OTEC) system. This system utilizes solar thermal energy as the secondary heat source. Various working fluids were also simulated to select one that is suitable for this system. The results showed that R152A, R600, and R600A, in that order, were the most suitable working fluids. The effective area of the solar collector for a $20^{\circ}C$ increase in the collector outlet temperature fluctuated from 50 to $97m^2$ owing to the change in the monthly average solar gain. The annual average efficiency of the SH-OTEC increases to 6.23%, compared to that of a typical conventional OTEC, which is 2-4%.

Synthesis and In Vitro Cytotoxic Activities of 2-Alkyl-2,3-dihydro-1H-2-azacyclopenta[b]anthracene-5,10-diones (2-알킬-2,3-다이하이드로-1H-2-아자사이클로펜타[b]안트라센-5,10-디온계 유도체 합성 및 세포독성)

  • Kwak, Jae-Hwan;Jeong, Eun-Kyeong;Oh, Ju-Hoon;Jung, Jae-Kyung;Hong, Jin-Tae;Lee, Hee-Soon
    • YAKHAK HOEJI
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    • v.53 no.1
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    • pp.41-44
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    • 2009
  • A series of 2-alkyl-2,3-dihydro-1H-2-azacyclopenta[b]anthracene-5,10-diones ($3a{\sim}h$) were synthesized and evaluated in vitro cytotoxicity against colon cancer cell lines (HCT116 and SW620) and nuroblastoma cell lines (SK-N-SH and SK-N-MC). Among them, compound 3f showed significant cytotoxic activity ($IC_{50}$ against SK-N-SH; $14.8{\mu}M$, $IC_{50}$ against SK-N-MC; $11.3{\mu}M$).

Evaluation of Resistance to Rice Sheath Blight (Rhizoctonia solani Kühn) of Rice Germplasms at Seedling Stage (유묘접종에 의한 벼 유전자원의 벼잎집무늬마름병 저항성 평가)

  • Kim, Jeong-Ju;Baek, Man-Kee;Won, Yong-Jae;Cho, Young-Chan;Kim, Bo-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.4
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    • pp.376-382
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    • 2013
  • Rice sheath blight (ShB), caused by Rhizoctonia solani K$\ddot{u}$hn, is one of the serious fungal disease resulting in severe yield loss in rice field worldwide. There are limited sources of genetic resistance and no commercial cultivar with high level of ShB resistance is currently available in Korea. In order to seek available resources with high level of resistance to ShB, 40 rice germplasms were used to evaluate disease reactions including ShB, leaf blast and bacterial leaf blight and these germplasms also examined agronomic traits such as days to heading, culm length, panicle length, No. of panicles, No. of spikelets per panicle and so on. There is wide variation in agronomic characters and disease reactions. Rice germplasms also showed considerably different ShB reaction caused by inoculation at seedling stage. Areumbyeo, Gayabyeo, IR579-Es44 and IR64 showed more strong reaction to ShB than the others. Especially, Gayabyeo is considerably available to develop a new variety with resistance to ShB in Korea.

Relationships between solar/interplanetary (IP) parameters and Dst index, according to IP sources

  • Ji, Eun-Young;Moon, Yong-Jae;Lee, Dong-Hun
    • Bulletin of the Korean Space Science Society
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    • 2010.04a
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    • pp.39.1-39.1
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    • 2010
  • We have investigated interplanetary (IP) structures of 82 intense geomagnetic storms (Dst $\leq$ -100 nT) that occurred from 1998 to 2006. According to their interplanetary origins, we classified them as four groups: 20 sMC events (IP shock and MC), 19 SH events (sheath field), 12 SH+MC events (Sheath field and MC), and 8 nonMC events (non-MC type ICME). For each group, we examined the relationships between Dst index and solar/IP parameters, namely, direction parameter (DP), CME speed ($V_{CME}$), solar wind speed ($V_{SW}$), minimum of IMF $B_z$ component($Bz_{min}$), and maximum of $E_y$ component ($Ey_{max}$).We found that the relationships strongly depend on their IP source. Our main results can be summarized as follows: 1) The correlation between Dst and DP is the best for the SH+MC events (r = -0.61). 2) The relationship between Dst and $V_{CME}$ gives the best correlation for the sMC events (r = -0.56). 3) There is the best correlation between Dst and $V_{SW}$ for the sMC events (r = -0.61), while there is a very weak correlation (r=-0.17) for the SH events. 4) The relationship between Dst and $Bz_{min}$ gives the best correlation (r = -0.87) for the SH+MC events. 5) The correlation between Dst and $Ey_{max}$ is the best for the SH+MC events (r = -0.87). Summing up, the sMC and SH+MC events give us good correlations, but the SH events, weak correlations. From this study, we suggest that this tendency should be caused by the characteristics of IMF southward components, e.g., smooth field rotations for the MC events and highly IMF fluctuations for the SH events.

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Culture Conditions Affecting the Molecular Weight Distribution of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Synthesized by Alcaligenaes sp. SH-69

  • Yoon, Joo Seok;Park, Sang Kyu;Kim Young Baek;Maeng, Hack Young;Rhee, Young Ha
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.279-283
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    • 1996
  • The weight average molecular weight of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesized by Alcaligenes sp. SH-69 was altered between $3.2$\times$10^5$ and $1.1$\times$10^6$ depending upon various culture conditions. It appeared that culture conditions favorable for the efficient production of copolyesters promoted the formation of higher molecular weight copolyesters. Polydispersity indices of isolated copolyesters were in the range of 1.5 to 2.5.

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Effects of Daegeum-eumja, Igwi-tang and Sihosogan-san on Gastric Mucosal Lesions Induced by Alcohol, Indomethacin and Burn-stress in Mice. (Alcohol, Indomethacin 및 Burn-stress로 유발된 생쥐의 위점막 손상에 대한 대금음자(對金飮子), 익위탕(益胃湯), 시호소간산(柴胡疎肝散)의 효과)

  • Kong, Kyung-Hwan
    • The Journal of Korean Medicine
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    • v.28 no.2 s.70
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    • pp.166-184
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    • 2007
  • Objectives : This study was conducted to investigate the effects of Daegeum-eumja, Igwi-tang, and Sihosogan-san on gastric mucosal lesions induced by alcohol, indomethacin, and burn-stress in mice. Methods : Experimental mice were divided into six groups. The normal group (NOR) did not receive any treatment to elicit gastropathy. In the control group (GE), gastropathy was elicited by alcohol, indomethacin, and stress. In the misoprostol group (MS), misoprostol was administered after gastropathy was elicited by alcohol, indomethacin, and stress. In the Daegeum-eumja group (DG), Daegeum-eumja was administered after gastropathy was elicited by alcohol, indomethacin, and stress. In the Igwi-tang group (IW), Igwi-tang was administered after gastropathy was elicited by alcohol, indomethacin, and stress. In the Sihosogan-san group (SH), Sihosogan-san was administered after gastropathy waselicited by alcohol, indomethacin, and stress. The effects on gastric mucosal lesions were evaluated by the morphological change of gastric mucosa, the anti-oxidant effect, HSP 70, $NF-{\kappa}B$ p65, $I{\kappa}B$, COX-1, PNA, BrdU, and iNOS. Results : Hemorrhage erosion, HSP70, and $NF-{\kappa}B$ in the DG, IW and SH groups decreased more than that of the control. The $I{\kappa}B$, COX-1, PNA, BrdU, and iNOS in the DG, IW, and SH groups increased more than that of the control. DG showed the most effect against gastric mucosal lesions induced by alcohol; IW against gastric mucosal lesions induced by indomethacin; and SH against gastric mucosal lesions induced by burn-stress. Conclusions: Daegeum-eumja, Igwi-tang, and Sihosogan-san extracts have excellent effects on gastric mucosal lesions induced by alcohol, indomethacin, and burn-stress, respectively.

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Biological Characteristics of the Shigella Species Isolated from Various Areas in Korea, 1985 (1985년 한국 각지에서 분리한 이질균속의 특성에 관한 연구)

  • Choi, Jae-Doo;Lee, Yun-Tai;Jung, Tae-Hwoa
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.1
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    • pp.79-93
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    • 1987
  • The result of various researches mainly in search of 194 Shigella strains, isolated by the Health Research Centers(situated in Seoul city, Inchon city, Pusan city, Kyonggi-Do, Kangwon-Do, Chungchongnam and Buk-Do, Kyongsangnam and Buk-Do, Jollanam and Buk-Do, and Jaeju-Do) in addition to those clinical laboratories of all the general hospitals situated down twon Seoul, conducted during the month of Jan. through Dec. 85, through the reisolating-activity program following its transportation into the laboratory, particularly for a complete check on its correctiveness, are as follows: 1. Isolation processes were performed with the 194 strains obtained from each placeduring the period of investigation: 164 Strains(84.5%) of Sh. flexneri, B group; 6 Strains(3.1%) of Sh. boydii, C group; 24 Strains(12.3%) of Sh. sonnei, D group, which means there's quite a lot in B group while Sh. dysenteriae, A group was not isolated at all. 2. The isolation rate of the 164, B group for subserotype was 1b, 84(51.2%) the highest one, 2(1.2%) on 3a the lowest one, 4, on C group; In D group subserotype II showed 14(58.4%) more than subserotype I. 3. The biological data on sexuality regarding the isolation-strain showed traditional particularity. But the subserotype 1b in B group 2(2.4%) showed gas-growth from glucose. In subserotype 1a, the indole-growth was 88.9% on masculine which was considerably a good one. In the test of arginine dihydrolase subserotype I among D group showed 100% masculine rate. The subserotype 6 among B group showed 92.5% masculine. In the dissolution test of manitol, all subserotypes showed 100% maculine except subserotype 1b. In the dissolutioning test of rhamnose, the subserotype I among D group showed 100% masculine which is the unusual one. 4. Interms of the area among 13 districts examined, Kangwon-Do had 41(21.1%) which is the highest one on its ratio. 5. In terms of season on the strain isolation category, 44(22.7%) is the number isolated in April which is the highest one. 6. In terms of ages, the strain isolation ratio was notably high above the ages of 60 which was 34(17.5%). Next one was 29(14.9%) which was under the ages of 4. 7. In terms of sex, female was 113 or 58.2% while male was 74 or 38.2%, which means the female had more than the male. 8. The result of the resisting capability on the usage of 12 antibiotic medication was; 100% on chloramphenicol; 94.3% on tetracycline, 82.0% on streptomycin, 76.3% on carbenicillin, 74.7% on ampicillin, in regular order. The strain source bearing multimedication resisivity against the 5 antibiotic medication is as many as 117 or 60.3%. Of which 43.3% of 1b sub serotype, B group was the best one, and thus the resistivity against the antistrain medication seems the tendency is being changed. The summing up of the above result shows the total specific strains isolated in each branch in Korea is 194, of which the main type is Sh. flexneri 84.5%. The isolating rate is almost evenly spreading, although the Kangwon-Do showed the highest rate on the above data. It also shows female is higher than male on its statistics. The tendency on age category showed both on old and infancy generations high. However, the resistant capability against antibacteria medication or vaccine was still remaining on habitual one, particularly tending towards multimedication or vaccine trend.

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