• 한국가축번식학회지
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• 제26권4호
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• pp.347-351
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• 2002

Transgenes in HSY-TK gene driven by the lck promoter was tested for the expression in immune cells (Jurkat cells) to apply xenotransplantation of human cells into transgenic animals for the potential use of the proliferation or differentiation of human stem cells in the large animal such as an pig. Also, lck-CFP gene was used for transfection experiment into Jurkat cell to confirm the proper regulation of lck promoter for transgene expression in the T cells. Transfection of lck-GFP gene into Jurkat ceils induced CFP expression in transfected cells. The expression of Ick-TK and Ick-CFP genes was confirmed by RT-PCR using RNAs extracted from Jurkat cells, When Jurkat cells transfected with TK and CFP genes were selected against G418 or gancyclovir treatments, Jurkat cells transfected with TK gene were not proliferated in G4i8 and gancyclovir medium while intact cells or cells transfected with CFP gene could grow in gancyclovir medium. However, Jurkat cells transfected with TK or GFP gene were proliferated in G418 medium probably due to Neo$^{r}$ gene in the vector. Gancyclovir treatment destroyed Jurkat cells expressing TK gene indicating that T-cells expressing TK gene can be selectively eliminated by TK gene expression driven by lck promoter.

• 한국식품영양과학회지
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• 제43권3호
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• pp.367-373
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• 2014

본 연구에서는 MG의 RC-58T/h/SA#4 인체 전립선 암세포에 대한 증식 억제 및 apoptosis 유도효과에 대하여 확인하였다. MG는 RC-58T/h/SA#4 세포의 증식을 농도 의존적으로 억제하였다. 이러한 MG에 의한 RC-58T/h/SA#4 세포의 사멸이 apoptosis에 의해 일어나는지를 sub-G1 함량 측정 및 Hoechst 33258 염색을 이용하여 확인하였다. 그 결과 MG를 처리한 군의 sub-G1의 함량이 대조군에 비하여 증가하였으며, methyl gallate를 처리한 군에서 핵의 응축과 apoptotic body 형성을 Hoechst 33258 염색을 통하여 관찰할 수 있었다. 또한 RC-58T/h/SA#4 세포에서 MG가 유도하는 apoptosis의 기전을 확인하기 위하여 다양한 실험을 실시하였다. MG는 DNA의 분절량 증가 및 caspase 활성을 유도하였으며, 활성화된 caspase-8, -9 및 -3에 의해 PARP와 Bid 단백질의 분절을 발현시켰으며, Bcl-2 family 단백질의 발현에 영향을 미쳐 apoptosis를 유도하였음을 확인하였다. 한편 환경호르몬인 dioxin과 bisphenol A를 다양한 농도로 처리한 결과 각각 1 nM, $0.1{\mu}M$ 농도에서 가장 높은 전립선 암세포 과다증식을 유도하였으며, 이를 바탕으로 MG를 농도별로 처리한 결과 환경호르몬에 의해 유도된 인체 전립선 암세포의 증식을 농도 의존적으로 억제시켰다. 본 연구 결과 MG는 RC-58T/h/SA#4 전립선 암세포에서 apoptosis 유도를 통한 암세포 성장 억제효과를 가지고 있음을 확인하였으며, 환경호르몬에 의해 유발될 수 있는 암에 대해서도 유사한 보호효과를 가지고 있음을 증명하였다.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2009년도 춘계 학술논문 발표대회 산업계
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• pp.1-6
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• 2009

The HwaSungSungYouk is one of the construction project in viewpoint of today's construction management and various information how to build the HwaSung at the last of the eighteen century had been recorded in HwaSungSungYoukEuGye. The purpose of this study is to analyse the data in HwaSungSungYoukEuGye in viewpoint of today's construction management for estimating the resources of PalDal-mun construction. In the record, there are a 10t of information relate to time management, cost management and human resource management, and the concept of modern construction management will be also use to figure out the construction data of the HwaSungSungYoukEuGye.

• 보건의료산업학회지
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• 제13권3호
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• pp.27-38
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• 2019

Objectives: The purpose of this study was to examine the influence of human rights sensitivity and nursing work environment on workplace bullying victimization among nurses in small- and medium-sized hospitals. Methods: The participants were 255 nurses from 5 general hospitals in Busan. A dataset was collected using a structured self-reporting questionnaire during the month of July 2018. The data were analyzed with SPSS WIN 23.0 software (IBM Corp., Armonk, NY, USA) using independent t-test, one-way analysis of variance, Pearson's correlation coefficient, and multiple regression analysis. Results: Nursing work environment showed negative correlation with workplace bullying victimization. Being a witness to bullying, organizational support, head nurse's leadership, and relationships with peers were found to influence workplace bullying victimization, and these 4 variables explained 37% of workplace bullying victimization. Conclusions: Formal procedures for cases of bullying and improvements in the leadership of head nurses and peer relationships are crucial to reducing workplace bullying victimization among nurses in small- and medium-sized hospitals. Hospital executives' efforts to provide sufficient physical and human resources for nursing services and to improve the welfare of nurses are also needed.

• International Journal of Quality Innovation
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• 제6권1호
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• pp.24-34
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• 2005

This research topic evaluates the effectiveness and importance of environmental protection in the 21st century in light of the increasing demand on the earth's natural resources and the pressures on economic and industrial development to provide dynamic power. As the world and our fellow citizens become more conscious of environmental protection, companies are under greater pressure whilst pursuing economic growth. Therefore, domestic manufacturers have been devoting efforts to promote environmental management. This research conducts survey using questionnaires on the operational performance of the manufacturers who have ISO 14000 series accreditation and certification. The survey considers five dimensions/functions within a manufacturer, financial management, human resources management, production management, and marketing management. A total of 35 indices are used for analysis of the effects that the location, history, industry, number of employees, amount of capital, and revenue may have on the performance. This research targets the manufacturers approved of ISO 14000 series certification by Environment Administration Association. The statistical methods deployed are descriptive statistics, T-test, and single factor analysis of variance used for analysis. The conclusions reveal that a certain level of performance has been achieved in every dimension. After T-test, all the indices have reached a significant level. The indications are that ISO 14001 benefits all manufacturers the level of benefits however varies from company to company.

• 한국수자원학회논문집
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• 제48권11호
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• pp.945-955
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• 2015

The technology of the SWG (Smart Water Grid) as one of most important national projects results in significant assignment that is closely associated with systematic management and effective operation. The individual component technics are required to establish directory and classification for the purpose of effectively managing their information related to research and development (R&D). The national science technology (S&T) standard classification tree which results in the representative example has been established with an intention to manage R&D information, human resource, and budget. It has been also revised every five years and then used in the various fields related to the evaluation, administration, and prediction of the national R&D projects. In addition, the standard classification system for R&D projects has been widely used in the UNESCO (United Nations Educational, Scientific and Cultural Organization) and EU (European Union) since the Frascati Manual was established in the Organization for Economic Cooperation and Development (OECD). Therefore, it is necessary for SWG techniques to develop the standard S&T classification tree for research management and evaluation. For this, it is essential to draw the core techniques for the SWG, which are incorporated with IT (Information Technology), NT (Nano Technology), and BT (Biology Technology).

• 한국자원식물학회지
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• 제32권2호
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• pp.109-115
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• 2019

이상의 연구 결과로 미루어 볼 때, 참식나무 기주 참나무겨우살이(TY-NS-B)는 $GSK3{\beta}$에 의한 ${\beta}$-catenin 인산화에 의존하지 않는 ${\beta}$-catenin의 분해 유도를 통해 암세포의 생육과 관련된 c-Myc의 발현을 억제하며, 이것은 비소세포성 폐암의 생육억제와 관련이 있는 것으로 판단된다. 또한, 본 결과는 항암을 위한 대체보완소재로 참식나무 기주 참나무겨우살이의 활용이 가능할 것으로 판단된다. 그러나 추가적 연구를 통해 참식나무 기주 참나무겨우살이가 어떻게 ${\beta}$-catenin의 분해 유도에 관여하는지에 대한 기전연구와 관련 활성물질의 분석연구가 필요할 것으로 사료된다.

• 한국가정과학회지
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• 제1권2호
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• pp.12-22
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• 1998

The purpose of this study was to examine the effects of householder's job status m the family life style. Data was collected from 475 parents in Chonbuk province by using a self administered questionnaire from June 10 to 30, 1998. Frequencies, percentages, and averages were calculated T-test, Pearson's correlation coefficients, and stepwise multiple regression analysis were followed. The results were as follows; 1. The families who suffered householders' job loss were 1/4 of the respondents and over 4/5 of the families felt economic stresses. 1/3 of the respondents had debts and 1/5 had not any savings. Most of the families were in good or normal marriage relationships, experienced economic crises, and had sparing habit. 2. In out-of-job families, couple cohension, couple satisfaction, and total marriage relationships were lower than in-job families. Also, out-of-job families spared more in buying and using clothes and foods, total clothes living and total food living. 3. In out-of-job families, the economic pressures on marriage relationships, clothes living, and food living were stronger than in-job families. 4. Family resources such as savings, ordinary marriage relationships, and sparing habit effected on the marriage relationships, clothes living, and food living in out-of-job families and in-job families. (Korean J Human Ecology 1(2):12∼22. 1998)

• Applied Biological Chemistry
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• 제47권2호
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• pp.202-207
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• 2004

어성초 분말을 포함하는 차가버섯 발효 조성물의 물 추출물이 인체 위암 세포 AGS 및 대장암 세포 HCT-15, 그리고 정상세포 NIH3T3 fibroblast의 세포 생육에 미치는 영향을 검토하였다. 세포독성 실험은 세포수 count와 MTT 방법으로 측정하였다. 어성초 분말을 포함하는 차가버섯 발효 조성물의 제조는 차가버섯과 어성초 분말을 혼합하고, 여기에 대두 발효 미생물원을 다시 혼합시켜 습도 $50{\sim}60%$,온도 $30{\sim}37^{\circ}C$에서 30일 정도 발효시킨 후 건조 시킨 분말에서 5% 물 추출물을 얻어 동결건조 시켜 실험에 제공하였다. MTT assay 방법에서 차가버섯 발효 조성물의 수용성 추출물 0.16, 0.4, 0.8, 1.6 및 4.0 mg/ml 첨가 농도에서 AGS 세포 생육은 13, 25, 40, 67 및78% 억제 되었으며, HCT-15세포 생육은 22, 40, 50, 69및 76%씩 각각 억제되었다. 그러나 동일한 실험조건에서 정상 세포수 NIH3T3은 86% 이상의 생존율을 나타내었다. 차가버섯 발효 조성물의 수용성 추출물은 인체 대장암 세포주 HCT-15와 위암 세포수 AGS에 대해 생육억제 작용이 강한 반면, 정상세포 NIH3T3에 대해서는 세포 독성을 거의 나타내지 않아 가장 바람직한 암예방 또는 항암식품 개발가능성을 제시하였다.

• 한국가정과학회지
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• 제5권1호
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• pp.1-13
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• 2002

The purpose of this study was to verify the effectiveness of the parent education program through play to improve the relationships between mothers and children. The subject of this study consisted of 30 mothers with 4-7 aged child attending S-day-care center located in Ik-San. In order to examine the effectiveness of the parent education program through play, two groups were divided into 15 participants each. The experimental group participated in the parent education program for total 8 sessions(2 sessions per week over the 4 weeks) while the control group didn't. The instrument were 'Maternal Affection & Control Resources Scale developed by Cho, Bok Hee(1991) and 'Teacher-Child relationship Scale' by Pinata & Steinberg. Teacher-Child relationship Scale was modified to be appropriate for use with this program. Parental acceptance level was measured by modified Parental Acceptance Scale (P.P.A.S.) by porter(1954). The data were analyzed by SPSSWIN program. The statistical methods for data analysis were frequency, t-test. paired t-test. The main results of this study were as follows : First, the mothers who participated in the parent education program through play showed improvement in affectionate attitudes than before. Second, the mothers who participated in the parent education program through play have been improved in familiarity and have been decreased in conflict of relationships of mother-child than those who did not participated in. Third, the mothers who participated in parent education program through play improve the recognition on respect to children's emotion, emotional expression right, uniqueness of children's temperament and needs to children's independency and autonomy.