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http://dx.doi.org/10.7732/kjpr.2019.32.2.109

Inhibitory Effect of the Branch Extracts from Taxillus yadoriki Parasitic to Neolitsea sericea against the Cell Proliferation in Human Lung Cancer Cells, A549  

Park, Su Bin (Department of Medicinal Plant Resources, Andong National University)
Kim, Ha Na (Department of Medicinal Plant Resources, Andong National University)
Kim, Jeong Dong (Department of Medicinal Plant Resources, Andong National University)
Park, Gwang Hun (Forest Medicinal Resources Research Center, National Institute of Forest Science)
Eo, Hyun Ji (Forest Medicinal Resources Research Center, National Institute of Forest Science)
An, Mi-Yun (Department of Medicinal Plant Resources, Andong National University)
Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University)
Publication Information
Korean Journal of Plant Resources / v.32, no.2, 2019 , pp. 109-115 More about this Journal
Abstract
In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of branches from Taxillus yadoriki parasitic to Neolitsea sericea (TN-NS-B) against human lung cancer cells, A549. TY-NS-B dose-dependently suppressed the growth of A549 cells. TY-NS-B decreased ${\beta}$-catenin protein level, but not mRNA level in A549 cells. The downregulation of ${\beta}$-catenin protein level by TY-NS-B was attenuated in the presence of MG132. Although TY-NS-B phosphorylated ${\beta}$-catenin protein, the inhibition of $GSK3{\beta}$ by LiCl did not blocked the reduction of ${\beta}$-catenin by TY-NS-B. In addition, TY-NS-B decreased ${\beta}$-catenin protein in A549 cells transfected with Flag-tagged wild type ${\beta}$-catenin or Flag-tagged S33/S37/T41 mutant ${\beta}$-catenin construct. Our results suggested that TN-NS-B may downregulate ${\beta}$-catenin protein level independent on $GSK3{\beta}$-induced ${\beta}$-catenin phosphorylation. Based on these findings, TY-NS-B may be a potential candidate for the development of chemopreventive or therapeutic agents for human lung cancer.
Keywords
Anticancer activity; ${\beta}$-catenin; Cancer chemoprevention; Cell growth; Taxillus yadoriki;
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