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Effect of Fermented Compositions Containing Inonotus obliquus with Houttuynia cordata on Growth of Human AGS Gastric and HCT-15 Colon Cancer Cells  

Cha, Jae-Young (Dept. of Biotechnolgy, College of Natural Resources and Life Science, Dong-A University)
Jeon, Beong-Sam (Dept. of Microbiology, College of Medicine, Gyeongsang National University)
Park, Jeong-Won (Dept. of Microbiology, College of Medicine, Gyeongsang National University)
Moon, Jae-Chul (Hong Jae Green Co., Ltd.)
Cho, Young-Su (Dept. of Biotechnolgy, College of Natural Resources and Life Science, Dong-A University)
Publication Information
Applied Biological Chemistry / v.47, no.2, 2004 , pp. 202-207 More about this Journal
Abstract
This study was performed to investigate the inhibitory effect of the water-extract from fermented compositions containing Inonotus obliquus with added Houttuynia cordata on the growth of either human AGS gastric and HCT-15 colon cancer cells or NIH3T3 normal mouse fibroblast cells. Cytotoxic activity on cancer cells was investigated by viable cell count, MTT assay and morphological observation. Mixtures of Inonotus obliquus with added Houttuynia cordata were fermented at $30{\sim}37^{\circ}C$, $50{\sim}60%$ humidity for 30 days, extracted with water, freeze dried, powered, and then dissolved in water for the experiment. In MTT assay, the fermented compositions exhibited inhibitory effects of 13, 25, 40, 67 and 78% for AGS and 22, 40, 50, 69 and 76% for HCT-15 at 0.16, 0.4, 0.8, 1.6 and 4.0 mg/ml, respectively. However, normal NIH3T3 cells were exhibited 86% survival under the same experimental condition. Fermented compositions showed highly inhibitory effect against human cancer cell line HCT-15 and AGS, but not on normal cell line NIH3T3.
Keywords
Inonotus obliquus; Houttuynia cordata; HCT-15; AGS; NIH3T3;
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