• Korean Journal of Animal Reproduction
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• v.26 no.4
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• pp.347-351
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• 2002

Transgenes in HSY-TK gene driven by the lck promoter was tested for the expression in immune cells (Jurkat cells) to apply xenotransplantation of human cells into transgenic animals for the potential use of the proliferation or differentiation of human stem cells in the large animal such as an pig. Also, lck-CFP gene was used for transfection experiment into Jurkat cell to confirm the proper regulation of lck promoter for transgene expression in the T cells. Transfection of lck-GFP gene into Jurkat ceils induced CFP expression in transfected cells. The expression of Ick-TK and Ick-CFP genes was confirmed by RT-PCR using RNAs extracted from Jurkat cells, When Jurkat cells transfected with TK and CFP genes were selected against G418 or gancyclovir treatments, Jurkat cells transfected with TK gene were not proliferated in G4i8 and gancyclovir medium while intact cells or cells transfected with CFP gene could grow in gancyclovir medium. However, Jurkat cells transfected with TK or GFP gene were proliferated in G418 medium probably due to Neo$^{r}$ gene in the vector. Gancyclovir treatment destroyed Jurkat cells expressing TK gene indicating that T-cells expressing TK gene can be selectively eliminated by TK gene expression driven by lck promoter.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.3
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• pp.367-373
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• 2014

In this study, we investigated the anticancer activity of methyl gallate (MG), which is the major biologically active component of Galla Rhois, in RC-58T/h/SA#4 human prostate cancer cells. MG inhibited cell proliferation in a dose-dependent manner. Cell death induced by MG increased the population of cells in sub-G1 phase, formation of apoptotic bodies, nuclear condensation, and DNA fragmentation. Apoptosis induced by MG was associated with activation of initiator caspases-8 and -9 as well as effector caspase-3. Endocrine disruptors such as dioxin and bisphenol A increased growth of RC-58T/h/SA#4 cells in charcoal-treated FBS (cFBS) medium. Cell proliferation was highest upon treatment with 1 nM and $0.1{\mu}M$ dioxin and bisphenol A, respectively. MG also dose-dependently inhibited cell proliferation in RC-58T/h/SA#4 cells treated with endocrine disruptors. These results indicate that MG exerts anticancer effects on RC-58T/h/SA#4 primary human prostate cancer cells.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2009.05c
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• pp.1-6
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• 2009

The HwaSungSungYouk is one of the construction project in viewpoint of today's construction management and various information how to build the HwaSung at the last of the eighteen century had been recorded in HwaSungSungYoukEuGye. The purpose of this study is to analyse the data in HwaSungSungYoukEuGye in viewpoint of today's construction management for estimating the resources of PalDal-mun construction. In the record, there are a 10t of information relate to time management, cost management and human resource management, and the concept of modern construction management will be also use to figure out the construction data of the HwaSungSungYoukEuGye.

• The Korean Journal of Health Service Management
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• v.13 no.3
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• pp.27-38
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• 2019

Objectives: The purpose of this study was to examine the influence of human rights sensitivity and nursing work environment on workplace bullying victimization among nurses in small- and medium-sized hospitals. Methods: The participants were 255 nurses from 5 general hospitals in Busan. A dataset was collected using a structured self-reporting questionnaire during the month of July 2018. The data were analyzed with SPSS WIN 23.0 software (IBM Corp., Armonk, NY, USA) using independent t-test, one-way analysis of variance, Pearson's correlation coefficient, and multiple regression analysis. Results: Nursing work environment showed negative correlation with workplace bullying victimization. Being a witness to bullying, organizational support, head nurse's leadership, and relationships with peers were found to influence workplace bullying victimization, and these 4 variables explained 37% of workplace bullying victimization. Conclusions: Formal procedures for cases of bullying and improvements in the leadership of head nurses and peer relationships are crucial to reducing workplace bullying victimization among nurses in small- and medium-sized hospitals. Hospital executives' efforts to provide sufficient physical and human resources for nursing services and to improve the welfare of nurses are also needed.

• International Journal of Quality Innovation
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• v.6 no.1
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• pp.24-34
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• 2005

This research topic evaluates the effectiveness and importance of environmental protection in the 21st century in light of the increasing demand on the earth's natural resources and the pressures on economic and industrial development to provide dynamic power. As the world and our fellow citizens become more conscious of environmental protection, companies are under greater pressure whilst pursuing economic growth. Therefore, domestic manufacturers have been devoting efforts to promote environmental management. This research conducts survey using questionnaires on the operational performance of the manufacturers who have ISO 14000 series accreditation and certification. The survey considers five dimensions/functions within a manufacturer, financial management, human resources management, production management, and marketing management. A total of 35 indices are used for analysis of the effects that the location, history, industry, number of employees, amount of capital, and revenue may have on the performance. This research targets the manufacturers approved of ISO 14000 series certification by Environment Administration Association. The statistical methods deployed are descriptive statistics, T-test, and single factor analysis of variance used for analysis. The conclusions reveal that a certain level of performance has been achieved in every dimension. After T-test, all the indices have reached a significant level. The indications are that ISO 14001 benefits all manufacturers the level of benefits however varies from company to company.

• Journal of Korea Water Resources Association
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• v.48 no.11
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• pp.945-955
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• 2015

The technology of the SWG (Smart Water Grid) as one of most important national projects results in significant assignment that is closely associated with systematic management and effective operation. The individual component technics are required to establish directory and classification for the purpose of effectively managing their information related to research and development (R&D). The national science technology (S&T) standard classification tree which results in the representative example has been established with an intention to manage R&D information, human resource, and budget. It has been also revised every five years and then used in the various fields related to the evaluation, administration, and prediction of the national R&D projects. In addition, the standard classification system for R&D projects has been widely used in the UNESCO (United Nations Educational, Scientific and Cultural Organization) and EU (European Union) since the Frascati Manual was established in the Organization for Economic Cooperation and Development (OECD). Therefore, it is necessary for SWG techniques to develop the standard S&T classification tree for research management and evaluation. For this, it is essential to draw the core techniques for the SWG, which are incorporated with IT (Information Technology), NT (Nano Technology), and BT (Biology Technology).

• Korean Journal of Plant Resources
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• v.32 no.2
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• pp.109-115
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• 2019

In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of branches from Taxillus yadoriki parasitic to Neolitsea sericea (TN-NS-B) against human lung cancer cells, A549. TY-NS-B dose-dependently suppressed the growth of A549 cells. TY-NS-B decreased ${\beta}$-catenin protein level, but not mRNA level in A549 cells. The downregulation of ${\beta}$-catenin protein level by TY-NS-B was attenuated in the presence of MG132. Although TY-NS-B phosphorylated ${\beta}$-catenin protein, the inhibition of $GSK3{\beta}$ by LiCl did not blocked the reduction of ${\beta}$-catenin by TY-NS-B. In addition, TY-NS-B decreased ${\beta}$-catenin protein in A549 cells transfected with Flag-tagged wild type ${\beta}$-catenin or Flag-tagged S33/S37/T41 mutant ${\beta}$-catenin construct. Our results suggested that TN-NS-B may downregulate ${\beta}$-catenin protein level independent on $GSK3{\beta}$-induced ${\beta}$-catenin phosphorylation. Based on these findings, TY-NS-B may be a potential candidate for the development of chemopreventive or therapeutic agents for human lung cancer.

• Korean Journal of Human Ecology
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• v.1 no.2
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• pp.12-22
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• 1998

The purpose of this study was to examine the effects of householder's job status m the family life style. Data was collected from 475 parents in Chonbuk province by using a self administered questionnaire from June 10 to 30, 1998. Frequencies, percentages, and averages were calculated T-test, Pearson's correlation coefficients, and stepwise multiple regression analysis were followed. The results were as follows; 1. The families who suffered householders' job loss were 1/4 of the respondents and over 4/5 of the families felt economic stresses. 1/3 of the respondents had debts and 1/5 had not any savings. Most of the families were in good or normal marriage relationships, experienced economic crises, and had sparing habit. 2. In out-of-job families, couple cohension, couple satisfaction, and total marriage relationships were lower than in-job families. Also, out-of-job families spared more in buying and using clothes and foods, total clothes living and total food living. 3. In out-of-job families, the economic pressures on marriage relationships, clothes living, and food living were stronger than in-job families. 4. Family resources such as savings, ordinary marriage relationships, and sparing habit effected on the marriage relationships, clothes living, and food living in out-of-job families and in-job families. (Korean J Human Ecology 1(2):12∼22. 1998)

• Applied Biological Chemistry
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• v.47 no.2
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• pp.202-207
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• 2004

This study was performed to investigate the inhibitory effect of the water-extract from fermented compositions containing Inonotus obliquus with added Houttuynia cordata on the growth of either human AGS gastric and HCT-15 colon cancer cells or NIH3T3 normal mouse fibroblast cells. Cytotoxic activity on cancer cells was investigated by viable cell count, MTT assay and morphological observation. Mixtures of Inonotus obliquus with added Houttuynia cordata were fermented at $30{\sim}37^{\circ}C$, $50{\sim}60%$ humidity for 30 days, extracted with water, freeze dried, powered, and then dissolved in water for the experiment. In MTT assay, the fermented compositions exhibited inhibitory effects of 13, 25, 40, 67 and 78% for AGS and 22, 40, 50, 69 and 76% for HCT-15 at 0.16, 0.4, 0.8, 1.6 and 4.0 mg/ml, respectively. However, normal NIH3T3 cells were exhibited 86% survival under the same experimental condition. Fermented compositions showed highly inhibitory effect against human cancer cell line HCT-15 and AGS, but not on normal cell line NIH3T3.

• Korean Journal of Human Ecology
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• v.5 no.1
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• pp.1-13
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• 2002

The purpose of this study was to verify the effectiveness of the parent education program through play to improve the relationships between mothers and children. The subject of this study consisted of 30 mothers with 4-7 aged child attending S-day-care center located in Ik-San. In order to examine the effectiveness of the parent education program through play, two groups were divided into 15 participants each. The experimental group participated in the parent education program for total 8 sessions(2 sessions per week over the 4 weeks) while the control group didn't. The instrument were 'Maternal Affection & Control Resources Scale developed by Cho, Bok Hee(1991) and 'Teacher-Child relationship Scale' by Pinata & Steinberg. Teacher-Child relationship Scale was modified to be appropriate for use with this program. Parental acceptance level was measured by modified Parental Acceptance Scale (P.P.A.S.) by porter(1954). The data were analyzed by SPSSWIN program. The statistical methods for data analysis were frequency, t-test. paired t-test. The main results of this study were as follows : First, the mothers who participated in the parent education program through play showed improvement in affectionate attitudes than before. Second, the mothers who participated in the parent education program through play have been improved in familiarity and have been decreased in conflict of relationships of mother-child than those who did not participated in. Third, the mothers who participated in parent education program through play improve the recognition on respect to children's emotion, emotional expression right, uniqueness of children's temperament and needs to children's independency and autonomy.