• Korean Journal of Korean Medical Institute of Dermatology and Aesthetics
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• v.1 no.1
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• pp.53-90
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• 2005

Purpose: Contents of astragaloside I, II and IV, cytotoxicity, anticancer activity, immunomodulatory activity and antioxidant capacity were to be compared as a function of the cultivated years as one, three, five and seven years. Method: Major components of Astragali membranacei Radix were separated as astragaloside I, astragaloside II, astragaloside IV by HPLC analysis. Cytotoxicity and anticancer activities were measured by MTT and SRB assay. For immunomodulatory activity, the secretion of IL -6 and $TNF-{\alpha}$, NK cell activation and macrophage activation were observed as well as kinetics of responding to human T cells by a microphysiometer. In vitro antioxidant activities were measured by several radical scavenging activities of superoxide anion radican, DPPH, LDL and linoleic acid. For in vivo activity, the activation of SOD, GSH-px, catalase, ALDH and ADH was measured as well the relative weight of liver. Result : 1. For HPLC analysis, the contents of all of astragaloside I, astragaloside II, astragaloside IV were in order of three, five, one and seven years. 2. The cytotoxicity of normal human lung cell line, HEL299 showed lower than 18% in adding 0.25 mg/ml, and 28.9% in adding 1.0 mg/ml of water extract of seven year root. For methanol extracts, three year root showed highest cytotoxicity as 35.2 % and there was no difference between the cultivated years. 3. For anticancer activities, methanol extracts of one and three year roots showed relatively high inhibition of human stomach cancer cells, AGS, breast cancer cells, MCF-7, lung cancer cells, A549 and liver cancer cell, Hep3B as well as high selectivities. 4. The water extract of seven year root could yield high secretion of IL-6 from both human Band T cells while the methanol extracts of three and five year roots secreted high amounts of IL-6 and $TNF-{\alpha}$ from both Band T cells. 5. As a result of in vitro antioxidant activities, both water and methanol extracts from five and seven year roots showed high activities for superoxide anion radical scavenging activity, inhibiting linoleic acid peroxide and contents of total phenols. 6. For in vivo tests, Mn-SOD and GSH-px activities and weight of liver were better in adding seven year root. For ALDH activity one year root was better and for ADH activity five year root. Overall speaking, seven year root showed relatively better antioxidant activities. Conclusion:There was difference of the contents of astragaloside I, astragaloside II, astragaloside IV according to cultivation year. Methanol extract showed better activities of anticancer and immune activation rather than water extract Interestingly enough, for methanol extracts, overall activities were improved as the cultivation year increased. There might be further investigation required for the clinical uses of the results as several biological activities varied according to the cultivated year of Astragali membranacei Radix.

• Journal of Bio-Environment Control
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• v.28 no.1
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• pp.9-15
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• 2019

This study aimed to examine the effects of different degrees of defoliation during harvest season on hydroponically cultured 'Seolhyang' strawberry's fruit characteristics, plant growth, and changes in nonstructural carbohydrates, and find an effective defoliation method. On November 29, 2013, some of the 'Seolhyang' leaves were non-defoliated and the others were defoliated remaining 9 and 5 leaves. The number of fruits and fruit weight were not significantly different in the first flower cluster but in the second, third, and fourth flower clusters decreased as the level of defoliation increased. The soluble solids content and acidity of fruits decreased in all the clusters as the level of defoliation increased. The leaf area and leaf dry weight of strawberry plant in all the treatment groups decreased from January to March and root dry weight sharply decreased during February. Thereafter, during April, the growth of plant increased. As defoliation increased, the dry weight of fruits, flower clusters, crowns, and roots decreased, and during late growing period, difference in dry weight according to the degree of defoliation was considerable. The content of carbohydrate was greater in the leaves than the fruits excepting January 30 and February 28, 2014 and in the case of strawberry plant that continuously produce fruits, the content of carbohydrate decreased in the leaves and roots. As defoliation increased, the content of carbohydrate in fruits, flower clusters, crowns, and roots decreased. Defoliation for strawberry plants is carefully done during harvest season. Twelve leaves during December and 14 leaves from January to March should be maintained, and in April when the number of leaves increases, old leaves should be defoliated.

• The Korea Journal of Herbology
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• v.27 no.6
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• pp.37-42
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• 2012

Objectives : Angelica Gigantis Radix is prescribed as the root of different Angelica species on the pharmacopoeia in Korea, Japan and China. Chemical components and their biological activities were also different according to their species. A study for the development of simple method to compare Angelica roots was needed. In order to classify them, the methods such as DNA sequencing analysis and taste sensor were applied to three Angelica species like Angelica gigas, Angelica acutiloba and Angelica sinensis. Methods : PCR amplification of intergenic transcribed spacer (ITS) region was performed using ITS1 and ITS4 primer from nine Angelica roots, and then nucleotide sequence was determined. Taste pattern of samples were measured using the taste-sensing system SA402B equipped with a sensing unit, which consists of artificial lipid membrane sensor probes of anionic bitterness, astringency, saltiness, umami, and cationic bitterness (C00, AE1, CT0, AAE, and AN0, respectively). Results : As a result of comparing the similarity of the ITS region sequences, A. sinensis was discriminated from the others (A. gigas and A. acutiloba). Equally this genetic result, A. gigas and A. acutiloba showed similar taste pattern as compared to A. sinensis. Sourness, bitterness, aftertaste of bitterness, astringency, and aftertaste of astringency of A. sinensis were significantly high as compared with A. gigas and A. acutiloba. In contrast, richness was significantly low. Conclusions : These taste pattern can be used as a way of comparison of Angelica species and this technic could be applied to establish a taste pattern marker for standardization of herbs in various purposes.

• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.357-362
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• 2009

Trials to find a method for overcoming the depletion of Tricholoma matsutake mycorrhiza from the artificially inoculated pine seedling (Pinus densiflora) when it is transplanted in situ were carried out. The culturing jars containing mycorrhizal seedlings were punched at the bottom to make the jar like a pot with keeping the soil structure of the original medium in vitro, and 8 kinds of irrigation solution were applied. Irrigation of distilled water caused disappearance of T. matsutake mycorrhizae, but biweekly irrigation of 2.5% PDMP (24 g potato dextrose broth, 3 g malt extract, 1 g peptone) solution or 0.5% Tween 80 solution until 3 months of acclimation could keep mycorrhizal association stronger. The percentage of mycorrhizal roots out of total length of roots was increased during the acclimation, and the acclimation rate reached up to 70%. Thus, we recommend that the mycorrhizal pine seedlings inoculated with T. matsutake would be acclimated before transplanting into the field.

• Journal of Plant Biology
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• v.34 no.2
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• pp.121-127
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• 1991

Total soluble proteins from three developmental stages of induced root nodules of Canavalia lineata were compared with those of non-nodulated roots by SDS-PAGE and two dimensional (2-D) gel electrophoresis. Thirteen nodule-specific protein (nodulin) bands were identified by the former and 30 nodule specific protein spots were detected by the latter method respectively. Some of the nodulins were detected differentially depending on the nodule's developmental stages. For example, only three leghemoglobin (Lb)-like protein spots appeared at stage I (d<2 mm), but two additional Lb-like protein spots appeared at stage II (d <4-5 mm). pI value and molecular weight of nomomers of Lb-like protein were narrower and greater than those of soybean, ranging from 4.4 to 5.0 and 15.7 kd respectively. Northern blot hybridization of total RNAs from roots and root nodules using soybean Lb cDNA as a probe made it clear that Lb gene was expressed tissue-specifically only in the root nodules.odules.

• Korean Journal of Plant Resources
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• v.13 no.1
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• pp.11-17
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• 2000

In an effort to produce high quality Angelica gigas in the southern area of Korea, a variety of mulching materials were used to prevent withering during the summer. The experimental results are summarized as follows. Soil water content and temperature under various mulching materials were higher than those without mulching, in all the cases studied. Among various mulching materials P. E.(polyethylene) mulching material showed the highest effect. The appearance of weeds was the lowest when using the black P. E. mulching, while growth of weeds was the greatest when using transparent mulching. Growth and development of the plant were better with P. E. mulching than straw mulching or no mulching, allowed for more and bigger leaves. The bolting rate was about 2∼5%, with a slightly larger value under P. E. mulching. Under P. E. mulching, probably due to good physical properties of the soil the number of roots was large, and the length and diameter were good. Active growth of roots under P. E. mulching resulted in a yield increase of the plant. The black P. E. mulching material showed a prominent effect on suppression of weed growth, and the black P. E. material was considered to be the most recommendable among various P. E. mulching materials when there is a lack of labor.

• Korean Journal of Pharmacognosy
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• v.39 no.4
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• pp.357-364
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• 2008

The roots of Saposhnikovia divaricata Schischk. (Umbelliferae) have been known to possess analgesic, anti-inflammatory, anti-parasitic and anti-bacterial activities, and used for curing headaches, fever and arthralgia. In this study, we aimed to isolate active constituents to provide phytochemical data for the quality control of this plant. Nine coumarins, eight chromones, three sterols and a coumarolignan were isolated from EtOAc-soluble fraction of the roots of S. divaricata through repetive column chromatography method using silica gel, ODS gel, Sephadex-LH 20, MPLC and HPLC. By analyses of spectroscopic data and comparison of their data with those of published values, the compounds were identified as 3'-O-angeloylhamaudol (1), ${\beta}$-sitosterol (2), marmesin (3), phellopterin (4), anomalin (5), imperatorin (6), xanthotoxin (7), deltoin (8), bergapten (9), stigmasterol (10), ledebouriellol (11), hamaudol (12), 8'-epicleomiscosin A (13), xanthoarnol (14), cimifugin (15), 5-O-methylvisamminol (16), daucosterol (17), 4'-O-${\beta}$-D-glucosyl-5-O-methylvisamminol (18), nodakenin (19), sec-O-glucosylhamaudol (20), prim-O-glucosylcimifugin (21). Among them, 8'-epicleomiscosin (13) was firstly reported from Umbelliferae family and xanthoarnol (14) and nodakenin (19) were isolated from this plant for the first time.

• Journal of Biosystems Engineering
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• v.17 no.3
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• pp.213-222
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• 1992

To develop the wireless remote opreating system of walking rice transplanter, this study was carried out for manufacturing the wireless remote control system acting upon the transplanter. The first report of this study contained about the construction and operating method of a developed remote control system for 2-row walking type transplanter. It is the second report about the field test for an experimental transplanter being attached a wireless control system and for a conventional transplanter. The test was executed to investigate the characteristics of velocity, rectilinearity, turning time, interval of roots and of rows, and field capacity in accordance with various engine speeds for an experimental transplanter. The measurements of this test were compared with those of the conventional transplanter. The obtained results were as follows : 1. The operating velocity of the experimental transplanter was faster than the conventional one when the high speed range of the engine but the variances were very large. 2. The rectilinearity, interval of raws and of roots, and the turning time of the experimental transplanter was inferior to the conventional one at some speed ranges of the engine. 3. Field capacity of the experimental transplanter was superior to the conventional one at high speed of the engine. 4. There were some disadvantages for the experimental transplanter to operate, because it was constructed without any aid devices, such as float and hydraulic system for instance. But if the remote control system of this study equip at the modern walking transplanter, it will be improved and utilized without defects which was revealed from this test.

• Imaging Science in Dentistry
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• v.44 no.2
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• pp.89-94
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• 2014

Purpose: Vertical root fracture (VRF) is a common complication in endodontically treated teeth. Considering the poor prognosis of VRF, a reliable and valid detection method is necessary. Cone beam computed tomography (CBCT) has been reported to be a reliable tool for the detection of VRF; however, the presence of metallic intracanal posts can decrease the diagnostic values of CBCT systems. This study evaluated and compared the effects of intracanal stainless steel or titanium posts on the sensitivity, specificity, and accuracy of VRF detection using a NewTom VG CBCT system. Materials and Methods: Eighty extracted single-rooted teeth were selected and sectioned at the cemento-enamel junction. The roots were divided into two groups of 40. Root fracture was induced in the test group by using an Instron machine, while the control group was kept intact. Roots were randomly embedded in acrylic blocks and radiographed with the NewTom VG, both with titanium and stainless steel posts and also without posts. Sensitivity, specificity, and accuracy values were calculated as compared to the gold standard. Results: The sensitivity, specificity, and accuracy of VRF diagnosis were significantly lower in teeth with stainless steel and titanium posts than in those without posts. Interobserver agreement was the highest in teeth without posts, followed by stainless steel posts, and then titanium posts. Conclusion: Intracanal posts significantly decreased the VRF diagnostic values of CBCT. The stainless steel posts decreased the diagnostic values more than the titanium posts.

• The Journal of Korean Medical History
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• v.33 no.1
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• pp.43-57
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• 2020

Essence of the Hwangchil tree (黃漆樹 Dendropanax morbiferus H.Lév.) has been used for various purposes, like waxing emperor's armors, covering an astrology board, or, during the Silla Dynasty, burying it to block something bad in the soil symbolically. Essence of the Hwangchil tree was known to have remarkable preservation effects such as waterproofing, damp-proofing, rust-proofing, and moth-proofing as well as not being easily peeled off from even soft surfaces like paper. There is a record in Prescriptions for Epidemic diseases of Cows, Horses, Sheep, and Pigs (牛馬羊猪染疫病治療方), published in 1541, of Hwangchil that is local to Jeju Island being used instead of benzoin (安息香), of burning Hwangchil, and of making cows inhale its smoke to prevent plague among them. Along the same lines, there are records in the Local Chronicle of Tamra (耽羅志) and the Book of Earth Geography (輿地圖書) that identify Hwangchil with benzoin. In Seonghosaseol (星湖僿說), a book written by Lee Yik in around 1760, it is acknowledged that Hwangchil could be medicinal herb. In 2000, Ahn Duk-Kyun registered the roots and branches of Hwangchil tree as 'Boncho' (本草 herbal medicine) in the Pictorial Book of Korean Medicinals (韓國本草圖鑑) and presented the method of taking it for medical purpose. Researchers have suggested that Hwangchil essence as well as diverse parts of the plant such as its roots, branches, leaves, flowers, fruits, and gum have various meaningful medicinal properties. Regarding the history and recent researches of using Hwangchil tree, it has various medicinal probabilities such as, 'dispersing miasma' (辟邪), 'opening holes' (開竅), 'waking the heart' (醒心), 'smoothing spirits' (安神), 'piercing the block' (疎泄), 'removing the old and welcoming the new' (去故生新). This paper contributes ideas about how to expand the uses of Hwangchil Tree.