The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Angelica Species Roots Using Taste Sensor and DNA Sequencing Analysis

미각센서와 DNA 염기서열을 이용한 당귀류 비교

  • Kim, Young Hwa (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Lee, Hye Won (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Lee, Gwan Ho (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Chae, Seong Wook (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Yun Hee (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Lee, Mi Young (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
  • 김영화 (한국한의학연구원 한의신약연구그룹) ;
  • 최고야 (한의기초연구그룹) ;
  • 이혜원 (한국한의학연구원 한의신약연구그룹) ;
  • 이관호 (한국한의학연구원 한의신약연구그룹) ;
  • 채성욱 (한국한의학연구원 한의신약연구그룹) ;
  • 김윤희 (한국한의학연구원 한의신약연구그룹) ;
  • 이미영 (한국한의학연구원 한의신약연구그룹)
  • Received : 2012.10.12
  • Accepted : 2012.11.05
  • Published : 2012.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Angelica Gigantis Radix is prescribed as the root of different Angelica species on the pharmacopoeia in Korea, Japan and China. Chemical components and their biological activities were also different according to their species. A study for the development of simple method to compare Angelica roots was needed. In order to classify them, the methods such as DNA sequencing analysis and taste sensor were applied to three Angelica species like Angelica gigas, Angelica acutiloba and Angelica sinensis. Methods : PCR amplification of intergenic transcribed spacer (ITS) region was performed using ITS1 and ITS4 primer from nine Angelica roots, and then nucleotide sequence was determined. Taste pattern of samples were measured using the taste-sensing system SA402B equipped with a sensing unit, which consists of artificial lipid membrane sensor probes of anionic bitterness, astringency, saltiness, umami, and cationic bitterness (C00, AE1, CT0, AAE, and AN0, respectively). Results : As a result of comparing the similarity of the ITS region sequences, A. sinensis was discriminated from the others (A. gigas and A. acutiloba). Equally this genetic result, A. gigas and A. acutiloba showed similar taste pattern as compared to A. sinensis. Sourness, bitterness, aftertaste of bitterness, astringency, and aftertaste of astringency of A. sinensis were significantly high as compared with A. gigas and A. acutiloba. In contrast, richness was significantly low. Conclusions : These taste pattern can be used as a way of comparison of Angelica species and this technic could be applied to establish a taste pattern marker for standardization of herbs in various purposes.

Keywords

References

  1. Korea Food and Drug Administration. The 9th Korean Herbal Pharmacopoeia. Seoul : Korea Food and Drug Administration. 2008 : 916.
  2. Ministry of Health. Labour and Welfare. The 16th Japanese Pharmacopoeia, Tokyo : Minister of Health, Labour and Welfare. 2011 : 1668.
  3. Chinese pharmacopoeia commission. The People's Republic of China pharmacopoeia, Beijing : China Medical Science and Technology Press. 2010 : 124.
  4. Han SB, Kim YH, Lee CW, Park SW, Lee HY, Ahn KS, Kim IH, Kim HM. Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai. Immunopharmacology. 1998 ; 40(1) : 39-48. https://doi.org/10.1016/S0162-3109(98)00026-5
  5. Cho CH, Kim SJ, Kim HJ. Comparative studies on the discrimination of Angelicae Gigantis Radix by near-infrared spectroscopy, electronic nose and X-ray fluorescence spectrometry. Yakhak Hoeji. 2002 ; 46(3) : 161-7.
  6. Sung JS, Bang KH, Park CH, Park CG, Yu HS, Park HW, Seong NS. Discrimination of Angelicae Radix based on anatomical characters. Korean J of Medicinal Crop Sci. 2004 ; 12(1) : 67-72.
  7. Seo YN. A study on the discrimination of Angelica species roots by dyeing. Korean J of Plant Res. 2007 ; 20(3) : 247-50.
  8. Kobayashi S, Putri SP, Yamamoto Y, Donghyo K, Bamba T, Fukusaki E. Gas chromatography-mass spectrometry based metabolic profiling for the identification of discrimination markers of Angelicae Radix and its application to gas chromatography-flame ionization detector system. J Biosci Bioeng. 2012 ; 114(2) : 232-6. https://doi.org/10.1016/j.jbiosc.2012.03.022
  9. Noh BS, Oh SY, Kim SJ. Pattern analysis of volatile components for domestic and imported Angelica gigas Nakai using GC based on SAW sensor. Korean J Food Sci Technol. 2003 ; 35(1) : 144-8.
  10. Park WS, Lee CH, Soh KS, Lee YJ, Lee CY, Lee TH, Kim YS, Kim DH. Study on biophoton emission from roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K. Korean J Herbol. 2007 ; 22(4) : 95-100.
  11. Lee JJ, Kim AR, Seo YN, Lee MY. Comparison of physicochemical composition of three species of genus Angelica. Korean J Food Preserv. 2009 ; 16(1) : 94-100.
  12. Choi HY, Choi YJ, Lee JH, Ham Ih. Sequencing analysis on the ITS region and AFLP analysis to identify dried medicinal Angelica species. Korean J Herbol. 2004 ; 19(2) : 91-9.
  13. Hosokawa K, Hishida A, Nakamura I, Shibata T. The sequences of the spacer region between the atpF and atpA genes in the plastid genome allows discrimination among three varieties of medicinal Angelica. Planta Med. 2006 ; 72(6) : 570-1. https://doi.org/10.1055/s-2005-916257
  14. Kobayashi Y, Habara M, Ikezazki H, Chen R, Naito Y, Toko K. Advanced taste sensors based on artificial lipids with global selectivity to basic taste qualities and high correlation to sensory scores. Sensors (Basel). 2010 ; 10(4) : 3411-43. https://doi.org/10.3390/s100403411
  15. Anjiki N, Hosoe J, Fuchino H, Kiuchi F, Sekita S, Ikezaki H, Mikage M, Kawahara N, Goda Y. Evaluation of the taste of crude drug and Kampo formula by a taste-sensing system (4): taste of Processed Aconite Root. J Nat Med. 2011 ; 65(2) : 293-300. https://doi.org/10.1007/s11418-010-0489-3
  16. Hayashi N, Chen R, Hiraoka M, Ujihara T, Ikezaki H. Beta-cyclodextrin/surface plasmon resonance detection system for sensing bitter-astringent taste intensity of green tea catechins. J Agric Food Chem. 2010 ; 58(14) : 8351-6. https://doi.org/10.1021/jf1012693
  17. Choi GY, Kim YH, Chae SW, Lee HW, Ko GS, Lee MY. Discrimination of Chinese Glycyrrhiza uralensis and Uzbek Glycyrrhiza glabra using taste sensor. Korean J Herbol. 2011 ; 26(1) : 35-9.
  18. White TJ, Bruns T, Lee S, Taylor T. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In : Innis MA, Gelfand DH, Shinsky JJ, White TJ eds. In PCR Protocols : A guide to methods and applications. San Diego : Academic Press. 1990 ; 315-22.
  19. Zhao KJ, Dong TT, Tu PF, Song ZH, Lo CK, Tsim KW. Molecular genetic and chemical assessment of radix Angelica (Danggui) in China. J Agric Food Chem. 2003 ; 51(9) : 2576-83. https://doi.org/10.1021/jf026178h
  20. Jeon WK, Yoo BK, Kim YE, Park SO, Parl SM, Ko BS. Effect of extracts for herbal medicines on the inhibition of whole blood aggregation. J Korean Soc Appl Biol Chem. 2007 ; 50(4) : 352-7.
  21. Choi MI, Lee HJ, Yun JB, Kim JY, Kang KS, Shin CG, Ju YS. The approach of properties-flavours theory and the study of morphological standard in CORNI FRUCTUS. Korean J Herbol. 2006 ; 21(2) : 109-19.