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Acclimation of Ectomycorrhizal Pine (Pinus densiflora) Seedlings Inoculated with Tricholoma matsutake by the Treatment of PDMP and Tween Solutions  

Park, Hyun (Korea Forest Research Institute)
Lee, Bong-Hun (Korea Forest Research Institute)
Ka, Kang-Hyeon (Korea Forest Research Institute)
Ryu, Sung-Ryul (Korea Forest Research Institute)
Bak, Won-Chull (Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.98, no.4, 2009 , pp. 357-362 More about this Journal
Abstract
Trials to find a method for overcoming the depletion of Tricholoma matsutake mycorrhiza from the artificially inoculated pine seedling (Pinus densiflora) when it is transplanted in situ were carried out. The culturing jars containing mycorrhizal seedlings were punched at the bottom to make the jar like a pot with keeping the soil structure of the original medium in vitro, and 8 kinds of irrigation solution were applied. Irrigation of distilled water caused disappearance of T. matsutake mycorrhizae, but biweekly irrigation of 2.5% PDMP (24 g potato dextrose broth, 3 g malt extract, 1 g peptone) solution or 0.5% Tween 80 solution until 3 months of acclimation could keep mycorrhizal association stronger. The percentage of mycorrhizal roots out of total length of roots was increased during the acclimation, and the acclimation rate reached up to 70%. Thus, we recommend that the mycorrhizal pine seedlings inoculated with T. matsutake would be acclimated before transplanting into the field.
Keywords
Tricholoma matsutake; Pinus densiflora; mycorrhiza; acclimation; PDMP; Tween 80;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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