• Title/Summary/Keyword: Root System

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The Danger-Associated Peptide PEP1 Directs Cellular Reprogramming in the Arabidopsis Root Vascular System

  • Dhar, Souvik;Kim, Hyoujin;Segonzac, Cecile;Lee, Ji-Young
    • Molecules and Cells
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    • v.44 no.11
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    • pp.830-842
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    • 2021
  • When perceiving microbe-associated molecular patterns (MAMPs) or plant-derived damage-associated molecular patterns (DAMPs), plants alter their root growth and development by displaying a reduction in the root length and the formation of root hairs and lateral roots. The exogenous application of a MAMP peptide, flg22, was shown to affect root growth by suppressing meristem activity. In addition to MAMPs, the DAMP peptide PEP1 suppresses root growth while also promoting root hair formation. However, the question of whether and how these elicitor peptides affect the development of the vascular system in the root has not been explored. The cellular receptors of PEP1, PEPR1 and PEPR2 are highly expressed in the root vascular system, while the receptors of flg22 (FLS2) and elf18 (EFR) are not. Consistent with the expression patterns of PEP1 receptors, we found that exogenously applied PEP1 has a strong impact on the division of stele cells, leading to a reduction of these cells. We also observed the alteration in the number and organization of cells that differentiate into xylem vessels. These PEP1-mediated developmental changes appear to be linked to the blockage of symplastic connections triggered by PEP1. PEP1 dramatically disrupts the symplastic movement of free green fluorescence protein (GFP) from phloem sieve elements to neighboring cells in the root meristem, leading to the deposition of a high level of callose between cells. Taken together, our first survey of PEP1-mediated vascular tissue development provides new insights into the PEP1 function as a regulator of cellular reprogramming in the Arabidopsis root vascular system.

A Standard Test Methods of Resistance to Root Penetration for Waterproofing and Rootproofing Membrane Using Green Roof System (인공지반녹화용 멤브레인 방수 및 방근재료의 방근성능 평가 방법 제안 연구)

  • Lee, Jung-Hoon;Seon, Yun-Suk;Kwak, Kyu Sung;Oh, Sang-Keu
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2009.11a
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    • pp.141-148
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    • 2009
  • The purpose of this paper is to propose a standard test methods of resistance to root penetration for waterproofing and rootproofing membrane using green roof system. Green roof system is considered to be an important subject in construction industry for green growth project. At the same time, we have to consider the counterplan for protection the damage of waterproofing layer and concrete substrate from the penetration of plant root. But many kinds of materials for protection from root penetration are using in construction field. But the performance of those materials is not clear, and there is not test methods for the evaluation of performance. So in this paper, based on the research results of 4 institutes during four years and foreign cases, we made a standard test methods of resistance to root penetration for waterproofing and rootproofing membrane using green roof system. This test method deals with about environmental condition of laboratory, experimental facilities, kinds of plant, specimen of test, management methods, evaluation duration and documents, etc.

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A Study on the Mock-up Test of Waterproofing/Root Penetration Resistance Technology for the Application to an Eco-bridge (생태교량 적용을 위한 방수·방근공법의 Mock-up 시험 연구)

  • Seon, Yun-Suk;Choi, Su-Young;Kim, Meong-Ji;Choi, Sung-Min;Oh, Sang-Keun
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2014.05a
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    • pp.230-231
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    • 2014
  • This study is concerned with the root penetration resistance competence and constructability of a waterproofing and root penetration resistance layer of an eco-bridge which is applied with various types of seeding. For the review of root penetration resistance competence, the competence was tested using herbs, woody plants, and tall trees. As a result, there was no tear of or damage to the waterproofing and root penetration resistance layer as well as the sweeping-down phenomenon of a waterproofing and root penetration resistance layer by soil. Also, the effect of the root growth of herbs, woody plants, and tall trees on the root penetration resistance system was confirmed.

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The Effect of Reinforcing Soil Shear Strength by a Root System Developed from Direct Sticking of Salix gracilistyla Miq (삽목에 의한 갯버들 근계의 토양전단강도 보강효과)

  • 이춘석;임승빈
    • Journal of the Korean Institute of Landscape Architecture
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    • v.31 no.5
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    • pp.1-10
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    • 2003
  • The purpose of this study was to verify the shore margin protection effect of a root system developed from direct sticking of Salix gracilistyla Miq., focusing on the reinforcement of soil shear strength. The materials were 20cm long sticks whose average diameter and weight were 7.52mm and 14.58g respectively, and sandy loam(Sand 60.36%, Silt 28%, Clay 11.64%), whose maximum dry weight(${\gamma}$$_{dmax}$) was 1.59gf/㎤ at the water ratio( $W_{opt}$) 13.8%. The direct shearing test(KS F 2343) was applied to cylindric columms(diameter 132mm) of pure soil and two years old root reinforced soil. At each condition of vertical stress, 10N/$ extrm{cm}^2$, 14.41N/$\textrm{cm}^2$ and 18.82 N/$\textrm{cm}^2$, five soil+root columns were sheared. After shear tests, the root area ratio and soil moisture on the shear plane were measured. The results of this research were as follows: 1. The average of root area ratio was 1.86% and the soil moisture 14.67%. 2. Two years old root system was found to increase the soil shear strength of pure soil in terms of Cohesion(C) and Inner friction Angle($\phi$) as follows. 3. The relationship between root area ratio and the increased shear strength can be presented with the following equation, $\Delta$S ≒ 0.33ㆍ TrㆍAs/A $\Delta$S : Increased Shear Strength Tr : Average Tension Strength of Root, Ar/A : Root Area Ratioioage Tension Strength of Root, Ar/A : Root Area Ratio

Obturation of the Cleaned and Shaped Root Canal System

  • Lee, Se-Joon
    • Proceedings of the KACD Conference
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    • 2001.11a
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    • pp.571-571
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    • 2001
  • The purpose of obturating the prepared root canal can be simply stated as to eliminate all avenues of leakage from the oral cavity or the periradicular tissues into the root canal system and to seal within the system any irritants that cannot be fully removed during canal cleaning and shaping procedures. The ability to achieve three-dimensional obturation of the root canal system is primarily dependent on the quality of the canal cleaning and shaping and the skill of the clinician.(omitted)

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LightSpeed and SimpliFil : Designed for Successful Endodontics

  • Senia, Steve
    • Proceedings of the KACD Conference
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    • 2001.05a
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    • pp.259-259
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    • 2001
  • ;A new root canal instrument and instrumentation technique: a preliminary report. Cleaning and shaping the root canal system has been and continues to be a challenge for even the most experienced endodontist. Curved. narrow canals. in particular. cause difficulties for the beginner as well as the specialist. A new instrument designed to incorporate new concepts was developed to ameliorate the problems in cleaning and shaping root canal systems. This new instrument has been given the name of SW (Senia and Wildey). The new SW instrument uses controlled right and left rotational forces. This motion was used to clean and shape simulated root canals in plastic blocks and root canals in extracted teeth. Instrumentation appeared to be easier. faster. and more precise than with conventional instruments. especially in curved canals where there was remarkable reduction of canal transportation. A mechanical version of the SW instrument was also developed. It was used to flare the coronal portion of the root canal system. Wildey WL. Senia ES., Oral Surg Oral Med Oral Pathol1989 Feb:67(2):198-207 Another look at root canal instrumentation. Several aspects of root canal instrumentation need additional research. Various factors must be considered in an analysis of instrumentation of the root canal system: the dentin that is cut: the technique used to cut it: the design of the instruments: the material and manufacturing process used to make the instruments: the irrigant used during the procedure: and the anatomic configuration of the root canal system. An analysis of these factors clearly indicates that existing root canal instruments and techniques are less than ideal and. in fact. do not accomplish what is expected of them. Root canals must be properly. but. at the same time. destructive and unnecessary removal of dentin should be kept to a minimum. The Flex-R and Canal Master instruments were developed to address some of the shortcomings of existing instruments and techniques. More scientifically based research is needed to fully evaluate these new instruments and techniques and to develop future instruments. Wildey WL; Senia ES. Montgomery S., Oral Surg Oral Med Oral Pathol1992 Oct:74(4):499-50799-507

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Performance Evaluation System for Construction Environment of the Unified Waterproofing-Root Resistance Membrane layer of the Green Roof System (인공지반 녹화용 방수방근 복합공법의 시공환경을 고려한 성능평가 시스템 연구)

  • Park, Chang-Hwa;Oh, Sang-Keun;Lim, Nam-Gi
    • Journal of the Korea Institute of Building Construction
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    • v.11 no.2
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    • pp.189-199
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    • 2011
  • Installation of a proper root barrier in a green roof system is very important in order to protect the concrete slab of roof and the root penetration in the waterproofing layer. To select the proper root barrier materials and methods, it is necessary to understand the environmental conditions affecting the waterproofing-root barrier system in green roof construction site. Therefore, we suggested as the environmental performance indexes four kinds of performance requirements; root penetration, chemical attack by chemical agent or fertilizer, load impact by soil depth and size of plant, and water pressure. The related four test methods were suggested for the inspection of these performance indexes. In this research, we could suggest for kinds of test methods as standard test methods to evaluate the environmental performance of waterproofing-root barrier for greening roof system.

CLEANSING EFFECT OF AIR-DRIVEN SONIC SYSTEM IN ROOT CANAL PREPARATION (공기진동(空氣振動) 근관형성법(根管形成法)의 근관정화효과(根管淨化效果))

  • Kim, Yong-Duk;Cho, Kyew-Zeung
    • Restorative Dentistry and Endodontics
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    • v.13 no.2
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    • pp.253-263
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    • 1988
  • In order to study the cleansing effect of air-driven sonic system, the author prepared root canals on 48 extracted human permanent single rooted teeth using hand instrumentation and aforementioned method, half and half of the cases. In order for comparison of the degree of remnant debris and cleanliness, the cross sections at 3mm and 7mm levels from the apex were stereomicroscopically observed and scored in accordance with predetermined degree. The obtained results were as follows: 1. Air-driven sonic system was more effective in cleansing root canal than hand instrumentation at apical 3mm level. (p < 0.01) 2. Air-driven sonic system was more effective in cleansing root canal than hand instrumentation at apical 7mm level. (p < 0.01) 3. In hand instrumentation, Helisonic file was more effective in cleansing root canal than Rispisonic file. (p < 0.05) In air-driven sonic system, on the other hand, there were no significant difference between the two files.

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Spot Cooling System Development for Ever-bearing Strawberry by Using Low Density Polyethylene Pipe (연질 PE관을 이용한 여름딸기 부분냉방기술 개발)

  • Moon, Jong Pil;Kang, Geum Choon;Kwon, Jin Kyung;Lee, Su Jang;Lee, Jong Nam
    • Journal of The Korean Society of Agricultural Engineers
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    • v.56 no.6
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    • pp.149-158
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    • 2014
  • The effects of spot cooling on growing ever-bearing strawberry in hydroponic cultivation during summer by spot cooling system was estimated in plastic greenhouse located in Pyeongchang. The temperature of cooling water was controlled by heat pump and maintained at the range of $15{\sim}20^{\circ}C$. Cooling pipes were installed in root zone and very close to crown. Spot cooling effect was estimated by applying system in three cases which were cooling root zone, crown plus root zone, and crown only. White low density polyethylene pipe in nominal diameter of 16 mm was installed on crown spot, and Stainless steel flexible pipe in nominal diameter of 15A was installed in root zone. Crown and root zone cooling water circulation was continuously performed at flowrates of 300 ~ 600 L/hr all day long. Strawberry yields by test beds were surveyed from Aug. 1 to Sep. 30. The accumulated yield growth rate compared with a control bed of crown cooling bed was 25 % and that of crown plus root zone cooling bed was 25 % and that of root zone cooling bed was 20 %. The temperatures of root spot in root zone cooling was maintained at $18{\sim}23.0^{\circ}C$ and that of crown spot in crown cooling was maintained at $19{\sim}24^{\circ}C$. Also, the temperatures of root spot in crown plus root zone cooling bed was maintained at $17.0{\sim}22.0^{\circ}C$ and that of crown spot was maintained at $19{\sim}25^{\circ}C$.

Literature Review of Construction Guide and Design Standard for Root Barrier Design of Green Roof System (인공지반녹화용 방근 설계 및 품질 향상을 위한 가이드라인 및 기준 고찰)

  • Suh, Jung-Il;Kim, Young-Sam;Shin, Hong-chol
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2019.11a
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    • pp.239-240
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    • 2019
  • The existing evaluation of root barrier resistance takes at least two years as an evaluation based on plant growth, and it is difficult to determine early performance in the process of quality control in the field. In this study, thus, the direction of the root barrier resistance evaluation system was presented by considering the construction specifications and design standards for root barrier design. As a result, it was necessary to establish a test method for reproducing simple and consistent test results for evaluating the durability of root barrier materials.

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