• 대한가정학회지
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• 제32권5호
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• pp.165-182
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• 1994

Korea's clothing industry which has been country's leading export industry and basic strategical industry is now faced with many difficulties both domestically and internationally. Domestically it is faced with continuing shortage of manpower in both production line and management high labour cost causing increase in price putting more weight on behavior of consumers resulting in change of industrial environment and continuing structural problems of industry itself. Internationally it is faced with strengthening of import regulations and protectionism of developed countries and rapid emergence of underdeveloped countries as leading exporting nations. In reality export plays the most essential role in our economy and is especially sensitive to the external environmental factors. Already economic bloc phonomenon can be seen everywhere and is continuing to accelerate in place such as E. U in Europ, North & South America as NAFTA, and South East Asian contries which recent tendency of economic unity effort is present. These countries of such economic blocs are imposing heavy custom duties reinforcing provision of country origin and acting out strict inspection regulations in order to protect the interest of their own industry. Therefore it is vital to manufacture excellent quality goods For these reasons study in this area has brought attention in Korea as well as worldwide in the recent years. Apparel industry which requires professional technology and ability is the most competitive international business. In order to challenge the international market the high level of intelligence is most required to produce high quality goods. The purpose of this study is to analyze the relationship between functions and roles of marketing and to approach problems in more efficient manner. Apparel industry is composed of various programs such as design pattern making merchandising and textile science. To succeed in the business is to give the highest satisfaction to the targeted market. Hence this study will example the factors that determine the Cost Quality and Performance of apparel products. The study will involve following steps; firstly establish relationship between the quality concept and productivity of apparel products Secondly inquire in to marketing strategy laying stress on apparel production related factors focusing on merchandising marketing production and operations Thirdly prospect 21st century apparel industry focusing on garment production and trade and also other countries structural improvement Fourthly establish the new dimension of competitive factors by grasping the actual circumstance of Korea's apparel industry in the international market. The research method will include; First reality approach method by analysing the present state of industry Second literal analysis such as marketing comparisons between leading apparel exporting countries.

• Tuberculosis and Respiratory Diseases
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• 제75권1호
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• pp.9-17
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• 2013

Background: In cancer cells, autophagy is generally induced as a pro-survival mechanism in response to treatment-associated genotoxic and metabolic stress. Thus, concurrent autophagy inhibition can be expected to have a synergistic effect with chemotherapy on cancer cell death. Monensin, a polyether antibiotic, is known as an autophagy inhibitor, which interferes with the fusion of autophagosome and lysosome. There have been a few reports of its effect in combination with anticancer drugs. We performed this study to investigate whether erlotinib, an epidermal growth factor receptor inhibitor, or rapamycin, an mammalian target of rapamycin (mTOR) inhibitor, is effective in combination therapy with monensin in non-small cell lung cancer cells. Methods: NCI-H1299 cells were treated with rapamycin or erlotinib, with or without monensin pretreatment, and then subjected to growth inhibition assay, apoptosis analysis by flow cytometry, and cell cycle analysis on the basis of the DNA contents histogram. Finally, a Western blot analysis was done to examine the changes of proteins related to apoptosis and cell cycle control. Results: Monensin synergistically increases growth inhibition and apoptosis induced by rapamycin or erlotinib. The number of cells in the sub-$G_1$ phase increases noticeably after the combination treatment. Increase of proapoptotic proteins, including bax, cleaved caspase 3, and cleaved poly(ADP-ribose) polymerase, and decrease of anti-apoptotic proteins, bcl-2 and bcl-xL, are augmented by the combination treatment with monensin. The promoters of cell cycle progression, notch3 and skp2, decrease and p21, a cyclin-dependent kinase inhibitor, accumulates within the cell during this process. Conclusion: Our findings suggest that concurrent autophagy inhibition could have a role in lung cancer treatment.

• 콘크리트학회논문집
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• 제15권2호
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• pp.288-296
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• 2003

최근 말뚝기초 공법으로서 강관말뚝의 활용이 토목 및 건축 분야에서 증대됨에 따라 이종의 재질을 가진 강관 말뚝과 콘크리트 확대 기초의 연결방법에 대한 기술개발 및 실제거동 파악에 대한 관심이 매우 높아지고 있다. 연결부에 대한 국내 시방서는 두 가지(A, B-type) 연결방법을 제시하고 있지만 실제적으로는 B-type 방법이 주로 활용되고 있는 상황이다. 본 논문에서는 강관 말뚝의 B-type 연결방법의 구조거동을 파악하기 위하여 말뚝에 작용하는 대표적인 하중인 연직하중에 대하여 3종류와 수평 하중에 대하여 두 종류의 실험체에 대한 재하시험을 실시하였다. 또한 재하실험 결과값에 대한 비교 분석을 위하여 3차원 유한 요소해석을 수행하였다. 실험과 수치해석 결과를 비교 분석한 바 현재 강관 말뚝 연결부의 설계법으로 적용되고 있는 가상 철근 콘크리트 기둥의 거동을 확인하였다. 또한 콘크리트 확대기초에 작용하는 하중을 강관으로 전달하기 위하여 사용되는 미끌림 방지턱의 역할과 이러한 방지턱이 강관과 콘크리트의 합성거동에 미치는 영향을 확인하였다. 본 연구를 통하여 향후 합리적인 강관 말뚝 연결부에 대한 설계 방법 및 기준을 정립함에 있어 기초 연구자료와 강관 말뚝 연결부의 구조거동을 파악하기 위한 실험방법을 제시하였다.

• BMB Reports
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• 제49권5호
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• pp.297-302
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• 2016

Loss of pancreatic β-cells by oxidative stress or cytokines is associated with diabetes mellitus (DM). DJ-1 is known to as a multifunctional protein, which plays an important role in cell survival. We prepared cell permeable wild type (WT) and mutant type (M26I) Tat-DJ-1 proteins to investigate the effects of DJ-1 against combined cytokines (IL-1β, IFN-γ and TNF-α)-induced RINm5F cell death. Both Tat-DJ-1 proteins were transduced into RINm5F cells. WT Tat-DJ-1 proteins significantly protected against cell death from cytokines by reducing intracellular toxicities. Also, WT Tat-DJ-1 proteins markedly regulated cytokines-induced pro- and anti-apoptosis proteins. However, M26I Tat-DJ-1 protein showed relatively low protective effects, as compared to WT Tat-DJ-1 protein. Our experiments demonstrated that WT Tat-DJ-1 protein protects against cytokine-induced RINm5F cell death by suppressing intracellular toxicities and regulating apoptosisrelated protein expression. Thus, WT Tat-DJ-1 protein could potentially serve as a therapeutic agent for DM and cytokine related diseases.

• BMB Reports
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• 제43권1호
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• pp.40-45
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• 2010

AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that plays a central role in cellular metabolic stress. Modulation of nitric oxide (NO) and cyclooxygenase-2 (COX-2) is considered a promising approach for the treatment of inflammation and neuronal diseases. In this study, the AMPK gene was fused in-frame with PEP-1 peptide in a bacterial expression vector to produce a PEP-1-AMPK fusion protein. Expressed and purified PEP-1-AMPK fusion proteins were transduced efficiently into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced PEP-1-AMPK fusion protein markedly inhibited LPS-induced iNOS and COX-2 expression. These results suggest that the PEP-1-AMPK fusion protein can be used for the protein therapy of COX-2 and NO-related disorders such as inflammation and neuronal diseases.

• 미생물학회지
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• 제50권1호
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• pp.27-32
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• 2014

자가포식현상(autophagy)은 세포 내 또는 세포 외의 스트레스나 영양분의 고갈, 그리고 병원체 감염에 의해 유도되는 기전으로, 병원균, 손상된 단백질이나 세포 소기관을 autophagosome으로 격리하여 리소좀(lysosome)과 융합하여 분해시키는 기전이다. Salmonella enterica serovar Typhimurium (S. Typhimurium)은 세포 내로 감염되는 세균으로 급성 위장염과 식중독을 야기한다. S. Typhimurium 감염 시 세포 내에서 자가포식현상이 유도되며 이는 감염을 제어하는데 중요하다는 연구 논문들을 통해 본 연구에서는 자가포식현상 유도제인 rapamycin으로 자가포식현상을 유도했을 때, S. Typhimurium의 감염을 조절할 수 있는지 알아보고자 하였다. 자가포식현상 유도제인 rapamycin과 저해제인 3-methyladenine(3-MA)를 각각 처리한 후 쥐의 큰포식세포주인 RAW 264.7 세포에 S. Typhimurium을 감염시켰다. rapamycin을 전처리한 후 S. Typhimurium을 감염시켰을 때, 세포 내에서 S. Typhimurium의 성장률이 감소한 반면 3-MA의 전처리는 S. Typhimurium의 성장을 촉진시켰다. 또한, RAW 264.7 세포에 rapamycin을 처리 후 감염시켰을 때, 자가포식현상 관련 단백질의 발현이 유의하게 증가하였다. Rapamycin에 의하여 유도된 자가포식현상이 활성산소종(reactive oxygen species, ROS)과 활성 산화질소종(nitric oxide, NO)의 생성을 통해 감염을 제어하는지를 확인하기 위하여 이 두 물질을 측정하였다. 감염 전 rapamycin 처리 시 RAW 264.7 세포에서 NO의 생성은 증가하였으나 ROS의 생성에는 별다른 차이가 없었다. 이상의 결과는 쥐의 큰포식세포주에서 rapamycin처리로 유도된 자가포식현상은 NO 생성을 통해 항박테리아능을 나타낸다고 할 수 있다.

• 한국수정란이식학회지
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• 제32권4호
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• pp.257-265
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• 2017

Ferulic Acid (FA) is a metabolite of phenylalanine and tyrosine, a phenolic compound commonly found in fruits and vegetables. Several studies have shown that FA has various functions such as antioxidant effect, prevention of cell damage from irradiation, protection from cell damage caused by oxygen deficiency, anti-inflammatory action, anti-aging action, liver protective effect and anti-cancer action. In this study, we investigated the maturation rate, intracellular glutathione (GSH) and reactive oxygen species (ROS) of porcine oocytes by adding FA to the in vitro maturation (IVM) medium and examined subsequent embryonic developmental competence at 5% oxygen through parthenogenesis. There is no significant difference between the control group ($0{\mu}M$) and treatment groups ($5{\mu}M$, $10{\mu}M$, $20{\mu}M$) on maturation rates. Intracellular GSH levels in oocyte treated with $5{\mu}M$ of FA significantly increased (P < 0.05), and $20{\mu}M$ of FA revealed significant decrease (P < 0.05) in intracellular ROS levels compared with the control group. Oocytes treated with FA exhibited significantly higher cleavage rates (79.01% vs 89.19%, 92.20%, 90.89%, respectively) than the control group. Oocytes treated with $10{\mu}M$ showed significantly higher blastocyst formation rates (28.3% vs 40.3%, respectively) after PA than the control group. Total cell numbers in blastocyst of $10{\mu}M$ FA displayed significantly higher (39.4 vs 51.9, respectively) than the control group. In conclusion, these results suggested that treatment with FA during IVM improved the developmental potential of porcine embryos by increasing intracellular GSH synthesis and reducing ROS levels. Also, there was an improvement of cleavage rate, blastocyst formation and total cell numbers in blastocysts. It might be associated with Keap1-Nrf2 pathway as an antioxidant regulate pathway that plays a crucial role in determining the sensitivity of cells to oxidative damages by regulating the basal and inducible expression of enzymes which is related to detoxification and anti-oxidative effects, stress response enzymes and/or proteins and ABC transporters.

• Plant Biotechnology Reports
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• 제3권2호
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• pp.147-155
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• 2009

Oryza grandiglumis Chitinase IVa (OgChitIVa) cDNA encoding a class IV chitinase was cloned from wild rice (Oryza grandiglumis). OgChitIVa cDNA contains an open reading frame of 867 nucleotides encoding 288 amino acid residues with a predicted molecular weight of 30.4 kDa and isoelectric point of 8.48. Deduced amino acid sequences of OgChitIVa include the signal peptide and chitin-binding domain in the N-terminal domain and conserved catalytic domain. OgChitIVa showed significant similarity at the amino acid level with related monocotyledonous rice and maize chitinase, but low similarity with dicotyledoneous chitinase. Southern blot analysis showed that OgChitIVa genes are present as two copies in the wild rice genome. It was shown that RNA expression of OgChitIVa was induced by defense/stress signaling chemicals, such as jasmonic acid, salicylic acid, and ethephon or cantharidin and endothall or wounding, and yeast extract. It was demonstrated that overexpression of OgChitIVa in Arabidopsis resulted in mild resistance against the fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. RT-PCR analysis showed that PR-1 and PR-2 RNA expression was induced in the transgenic lines. Here, we suggest that a novel OgChitIVa gene may play a role in signal transduction process in defense response against B. cinerea in plants.

• Applied Microscopy
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• 제37권3호
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• pp.167-174
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• 2007

Heat shock protein 70 (HSP70)은 다양한 질병상태와 치명적인 열 손상에서 세포 및 조직을 보호하는데 중요한 역할을 하며, 또한 외부의 stress로부터 세포내 단백질의 파괴와 변화를 감소시키는 단백질로 알려져 있다. 본 연구에서는 오랜 기간 동안 조혈기관의 치료제로 콩팥에서도 세포보호효과가 있는 것으로 알려진 Erythropoietin(EPO)을 투여하여 콩팥내의 HSP70의 발현변화를 세포수준에서 관찰하고자 하였다. Sprague-Dawley계 흰쥐를 사용하여 전자현미경적 면역조직화학법으로 rHuEPO투여군과 대조군에서 HSP70의 발현변화를 관찰하였다. 대조군에서 HSP70은 콩팥의 바깥수질과 속수질에서 관찰되었으며, 특히 속수질에서 강하게 발현되었고 그 부위는 속수질집합관세포와 헨레고리의 내림가는 부분이었다. EPO 투여군에서는 속수질과 바깥수질의 내림가는 부분에서는 발현변화가 관찰되지 않았으나, 바깥수질의 집합관 세포에서 발현이 급격히 증가함을 관찰되었다. 특히 대조군에서의 핵주변부위뿐 아니라 세포내 핵상부분을 비롯한 세포막주변부위에도 강한 면역 염색성을 나타내었다. 이러한 결과는 콩팥의 바깥수질에서 stress성 단백질인 HSP70의 조절기전이 EPO에 의해 매개됨을 보여주면, 세포 stress및 질병상태에서도 이러한 기전이 작용할 것으로 생각된다.

• 정보화정책
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• 제26권4호
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• pp.36-61
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• 2019

스마트기기는 업무의 시공간적 제약을 줄이고 신속한 처리를 가능하게 함에 따라 업무 효율성 및 생산성에 긍정적인 영향을 줄 수 있는 반면에, 직장과 개인생활 간 불균형 초래로 인한 부작용이 뒤따를 수 있다. 최근 스마트기기의 업무활용이 증가하면서 그 어느때보다도 관련 현상의 이해와 원인 발견, 그에 맞는 적절한 방안들을 모색하는 것이 필요한 상황이다. 본 연구는 스마트기기의 효용성을 대표하는 기술적 특성('IT기반 업무 유연성')이 '일-삶 갈등'과 같은 부정적인 결과로 어떻게 전환되는지를 보여주는 이론적 모델을 개발하고 이를 검증하였다. 직장인을 대상으로 수집된 데이터를 분석한 결과, 주요 요인들('IT기반 업무 유연성', '근무시간 외 업무 유연성', '업무 과부하', '일-삶 갈등') 간 다양한 영향 관계, 그리고 ''일-삶 분리' 조직 지원'의 중요성과 관련하여 연구자는 물론이고 현업 종사자와 정책 입안자들에게도 의미있는 시사점이 도출되었다.