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http://dx.doi.org/10.5483/BMBRep.2010.43.1.040

Transduced PEP-1-AMPK inhibits the LPS-induced expression of COX-2 and iNOS in Raw264.7 cells  

Shin, Min-Jea (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
Lee, Yeom-Pyo (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
Kim, Dae-Won (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
An, Jae-Jin (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
Jang, Sang-Ho (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
Cho, Sung-Min (Department of Neurosurgery, Hallym University Medical Center)
Sheen, Seung-Hoon (Department of Neurosurgery, Hallym University Medical Center)
Lee, Hae-Ran (Department of Pediatrics, Hallym University Medical Center)
Kweon, Hae-Yong (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA)
Kang, Seok-Woo (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA)
Lee, Kwang-Gill (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA)
Park, Jin-Seu (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
Eum, Won-Sik (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
Cho, Yong-Jun (Department of Neurosurgery, Hallym University Medical Center)
Choi, Soo-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
Publication Information
BMB Reports / v.43, no.1, 2010 , pp. 40-45 More about this Journal
Abstract
AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that plays a central role in cellular metabolic stress. Modulation of nitric oxide (NO) and cyclooxygenase-2 (COX-2) is considered a promising approach for the treatment of inflammation and neuronal diseases. In this study, the AMPK gene was fused in-frame with PEP-1 peptide in a bacterial expression vector to produce a PEP-1-AMPK fusion protein. Expressed and purified PEP-1-AMPK fusion proteins were transduced efficiently into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced PEP-1-AMPK fusion protein markedly inhibited LPS-induced iNOS and COX-2 expression. These results suggest that the PEP-1-AMPK fusion protein can be used for the protein therapy of COX-2 and NO-related disorders such as inflammation and neuronal diseases.
Keywords
AMPK; Cyclooxygenase-2 (COX-2); Inducible nitric oxide synthase (iNOS); Lipopolysaccaride (LPS); Protein transduction;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
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