• Korean Journal of Plant Tissue Culture
• /
• v.25 no.3
• /
• pp.183-187
• /
• 1998

Chlorophyll mutants were produced by treating the rhizome of Cymbidium kanran with mutagen, EMS(ethyl methan sulfonate). The germination ratio of Cymbidium kanran seeds was 5.5 times higher when the seeds were treated with ultrasonic treatment for 20 minutes than untreated control. Fifty to sixty percent of the rhizomes became dark brown when they were cultured in a liquid growth medium containing 0.2% EMS for three weeks. When the dark-brown rhizomes were cultured in a solified MS medium, new rhizomes were developed from a part of the old ones. Chlorophyll mutant rhizomes were obtained from a meristem tissue by a subculturing the cuts of these new rhizomes for a year. Of the chlorophyll mutants, a zigzag-striped type of rhizome was dominant and light-yellow and albino ones were also produced. While the zigzag-striped type rhizomes were differentiated into green and striped plant, the light yellow and the white rhizomes produced yellow-striped and albino plants repectively.These results indicate that the EMS treatment on the rhizome is an effective means to induce a chlorophyll mutant. We believe that this method may be useful to produce variegated plants chlorophyll mutants from other orchids.

• Korean Journal of Plant Resources
• /
• v.12 no.4
• /
• pp.276-281
• /
• 1999

This study was conducted to determine the appropriate transplanting time for growing one year old rhizome of Atractylodes macrocephala Koidz. Transplanting on March 20 and April 5 showed the highest in plant height and shoot fresh weight but dry rhizome weight was the highest in the transplanting time of April 5. As the growth time of 209 days showed was the highest yield of dry rhizome. As a result, it seemed that the appropriate planting time was April 5 to 10 in the north area of the Kyongsangbuk-do. The shortest emergence time was that the accumulated temperature of early growth period was 267$^{\circ}C$, and that of highest dry rhizome yield was 3$600^{\circ}C$ for whole growth period.

• Journal of Ginseng Research
• /
• v.21 no.3
• /
• pp.187-195
• /
• 1997

The Chinese ginseng roots were collected at twelve places of Jilin Province and two places of Liaoning Province in China and their appearances were compared with those of the Korean ginseng roots. The color of the most of the Chinese red ginseng was brown or dark brown and the color of many of the Chinese dried ginseng was pale yellow and the root-age of the most of the Chinese red ginseng as well as the Chinese dried ginseng was evaluated five or six year regardless of the collection places, so it cannot be easily concluded that the color and the root-age of the Chinese ginseng roots are different from those of the Korean ginseng roots. However the rhizomes and the lateral roots of the Chinese ginseng roots were poorly developed and many of them did not have either rhizome or lateral roots. Moreover the rhizomes of the Chinese red ginseng as well as the Chinese dried ginseng were much more easily removed than those of the Korean red ginseng and the Korean white ginseng. Therefore it is thought that the development status of the rhizome and the lateral roots of the Chinese ginseng roots are quite different from those of the Korean ginseng roots.

• Natural Product Sciences
• /
• v.17 no.4
• /
• pp.285-290
• /
• 2011

The rhizome of Alisma orientale Juzep (Alismataceae) has been used as a crude drug for diabetes, edema, inflammation and urinary disturbances in oriental medicine. Recent animal studies have shown that the extract of Alisma orientale rhizome (AOR) can potently lower high levels of serum lipids and improve insulin resistance, which are usually detected in patients and animals with non-alcoholic fatty liver disease. So, we studied the antioxidative effects of AOR extracts and fraction in vitro and their protective effects against acute hepatotoxicity induced by $CCl_4$ in vivo.. We then investigated the effects of each fraction on hepatotoxicity induced by tert-butyl hydroperoxide (t-BHP). DAOR (dichloromethane fraction of the Alisma orientale rhizome) scavenged free radicals and superoxide anions. DAOR protected against $CCl_4$ induced hepatotoxicity. DAOR had hepatoprotective and antioxidative effects against t-BHP-induced hepatotoxicity in HepG2 cells and in rats.

• Korean Journal of Plant Resources
• /
• v.16 no.2
• /
• pp.118-122
• /
• 2003

These studies were carried out to clarify the effects of black P.E. mulching and planting density on the growth and rhizome yield of Jihwang #1, a new high yielding Rehmannia glutinosa variety. Emergence ratio was higher 3.1％ in mulching than in non-mulching, but was not affected by planting density. Plant height and fresh leaf weight per plant were increased by mulching and at lower planting density. However, the fresh leaf weight per unit area was increased at higher planting density. Dry rhizome weight was increased 28% in mulching than that of non-mulching. Dry rhizome weight per plant was increased, but that of per unit area was decreased at lower planting density.

• Korean Journal of Pharmacognosy
• /
• v.13 no.4
• /
• pp.145-152
• /
• 1982

Three species of genus Polygonum, namely P. cuspidatum, P. sachalinense and P. ellipticum are distributed in Korea. Polygonum ellipticum Migo is a perennial herb in Polygonaceous plants. The root of the plant (Polygoni Rhizoma, 'Ho-jang') have been used as laxative, diuretic and for the treatment suppurative dermatitis in the oriental medicine. As the part of the study for the comparison of the three species in their components, the authors attempted to isolate the anthraquinones and stilbene derivative from the rhizome of P. ellipticum. The methanolic extract of dried rhizome of this plant was fractionated into ether soluble and insoluble fraction and each fraction was applied to column chromatography to isolate above mentioned components. Anthraquinone derivatives were isolated first; comp. I, mp $204{\sim}205^{\circ}$ (physcion), comp. II, mp $254{\sim}255^{\circ}$ (emodin), comp. IV, mp $191{\sim}192^{\circ}$ $(emodin-8-O-{\beta}-D-glucoside)$ and comp. III, mp $280{\sim}282^{\circ}$ $({\beta}-sitosterol-glucoside)$. They were identified by chemical properties and UV, IR and NMR spectra and by the direct comparison with authentic samples. Stilbene derivative was isolated secondly; comp. V, mp $255{\sim}256^{\circ}$ which was reported to possess antibacterial and antifungal activities.

• Food Science and Biotechnology
• /
• v.14 no.5
• /
• pp.685-688
• /
• 2005

Antibacterial and antiplatelet activities of Acorus gramineus rhizome-derived asaronaldehyde and asaron were analyzed using platelet aggregometer and six human intestinal bacteria. Active constituent of A. gramineus rhizome was isolated and characterized as asaronaldehyde by spectral analyses. At 2 and 1 mg/disk, asaronaldehyde exhibited strong inhibition of Clostridium perfringens and C. difficile without adverse effects on growth of beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus acidophilus, and L. casei. Asaron also revealed moderate growth inhibition against C. perfringens and C. difficile at 2 mg/disk, no growth-inhibiting activity was observed on B. bifidum, L. acidophilus, L. casei, and E. coli. At 50% inhibitory concentration ($IC_{50}$) value, asaronaldehyde was effective in inhibiting platelet aggregation induced by collagen ($IC_{50}$, $27.6\;{\mu}M$) and arachidonic acid ($IC_{50}$, $53.7\;{\mu}M$). These results suggest asaronaldehyde may be useful as lead compound for inhibiting platelet aggregation induced by collagen and arachidonic acid.

• Journal of Wetlands Research
• /
• v.14 no.2
• /
• pp.193-198
• /
• 2012

To enhance the proliferation efficiency of Menyanthes trifoliata L. endangered species in Korea, various rhizome cuttings were tested based on cutting length and node numbers. Growth length and increased volume of rhizomes were highest on long cuttings of 10-18cm length. But relative growth length of rhizomes were higher in short cuttings of 2-4cm and medium cutting of 4-10cm length than in long cuttings. Relative increased volume of rhizomes was highest in medium cuttings. The node numbers were not contributed to the efficiency of relative growth of rhizomes. This study suggests that medium length cutting is the best practice for the proliferation of M. trifoliata.

• Food Science and Biotechnology
• /
• v.16 no.2
• /
• pp.290-293
• /
• 2007

Cassumunar ginger (Zingiber montanum Roxb.) was grown in the experimental field at the Department of Horticulture, Kasetsart University, Thailand. The antioxidant activity and volatile oil content of rhizomes of varying age were measured. Antioxidant activity as determined using the DPPH (diphenyl-2-picrylhydrazyl) method differed significantly between samples of different ages. Antioxidant activity and rhizome age were positively correlated, with 22-month old rhizomes showing the highest radical scavenging activity (79.19%). Volatile oil was obtained by steam distillation of fresh rhizomes. The extraction yield of volatile oil was highest in l6-month old rhizomes (13.02 mL/kg). GC-FID data indicated the presence of three major compounds, sabinene, terpinen-4-ol and (E)-1-(3',4'-dimethylphenyl) butadiene (DMPBD), however none of the major components were correlated with the age of rhizome.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.5 no.1
• /
• pp.59-65
• /
• 2002

The community of common reed (Phragmites australis) is expanded by the development of new shoots from nodes of rhizomes and old shoots in natural environments. We developed the useful technique to construct reed mats of floating islands developed from shoot nodes of reeds for the purpose of the rapid stabilization of vegetation and the application of adapted plants with their specific environmental conditions. The vegetation development was compared in the floating islands planted with the different reed samples. The reed samples were consisted of four types : long shoot in the length of 100 cm (AG I), short shoot of 15 cm (AG II), long rhizome of 100 cm (BG I) and short rhizome of 15 cm (BG II). Shooting started earlier in BG I and BG II than in AG I and AG II. But the rate of shooting was higher in AG I and AG II than in BG I and BG II. After four weeks, AG I and AG II were higher than BG I and BG II in the biomass and density of newly developed shoots. These results showed that the construction of sod mats by planting only reed shoots was a useful technique to develop vegetation on the floating islands.