• Journal of Life Science
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• v.25 no.1
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• pp.29-36
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• 2015

Prodigiosin, a member of natural red pigment family, is produced by Serratia marcescens, and characterized by a common pyrrolylpyrromethane skeleton. This pigment has been reported with the effects of anticancer, immunosuppressant, antifungal, and algicidal activities. Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital infections. In this study, anti-MRSA properties of prodigiosin isolated from Serratia sp. PDGS 120915 were investigated. We identified and purified prodigiosin using high performance liquid chromatography (HPLC) and evaluated anti-MRSA activity. Purified prodigiosin inhibited the growth of MRSA. The minimum inhibitory concentrations (MICs) of prodigiosin were determined to $32{\mu}g/ml$ against the MRSA strains. Fractional inhibitory concentration (FIC) indices of ampicillin and penicillin were indicated synergistic effects of prodigiosin on MRSA.

• Korean Journal of Clinical Laboratory Science
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• v.39 no.1
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• pp.7-18
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• 2007

A total of 1,780 isolates of Enterococcus spp. were isolated from 63,133 clinical specimens from Dec 1, 2005 to Nov 1, 2006 in "C" hospital. Isolation frequencies of Enterococcus spp. were 50.9% for E. faecalis, 41.7% for E. faecium, and 7.4% for other Enterococcus spp. containing E. avium, E. gallinarum, E. casseliflavus, E. durans, E. hirae, and E. raffinosus. There were no significant difference between gender, but according to the age group analysis, Enterococcus spp. were more frequently isolated in patients over 50 years old (20.0~24.6%) than those isolated from the patients under the age of 0~49 (1.3~9.4%). In monthly analysis, Enterococcus spp. were the most frequently isolated in April (11.9%), but presented at lowest frequency in February (5.2%). Seasonal analysis did not show a significant difference. Over half of enterococci were isolated from random urine (44.9%) and catherterized urine (15.7%). Frequencies of vancomycin resistant E. faecalis and E. faecium were 0.1% and 31.0%, respectively. Teicoplanin resistant Enterococcus was 13.3% in E. faecalis, 17.6 % in E. faecium. The Enterococcus species showing over 80% susceptibility against antimicrobial agents were E. faecalis, E. durans and E. hirae in vancomycin; E. faecalis, E. gallinarum, E. casseliflavus, E. durans and E. hirae in ampicillin. The antimicrobial agent showing susceptibility against whole group of Enterococcus species was only linezolid (95.9%), and a selection of antimicrobial agent is necessary to do essential performance identification and susceptibility tests.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2003.05a
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• pp.139-142
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• 2003

Traditionally, the thickness of fire protection materials of structural elements such as beam and column have been decided by fire test using the predominant steel section of $H-300{\times}300{\times}10{\times}15$ for column and $H-400{\times}200{\times}8{\times}13$ for beam in Korea. But this way of determination of fire protection thickness yields very unduly results. Because the temperature-increment rate of structural steel elements depends mainly on magnitude of their cross-areas. In general, the thicker size of cross-areas for structural elements, the lower temperature shows up. It had already proved that the fire protection thickness only depends on the size of cross-areas and the fire protection method for three-fide or four-side exposed conditions in European countries, the United State of America and so on. To demonstrate there would be differences among various cross-areas for structural elements, we conducted several fire tests with full-scale specimens of beams and columns. For the determination of critical temperature for steel section when the fire resistant performance is needed to be decided, we conducted with a loaded fire test for beam and column, respectively. The small column in 1.0 meter length and beam in 1.5 meter length were used in order to deprive the rational fire protection thickness of structural elements such as beam and column, respectively. After test, we could obtain there were significant temperature lass between higher cross-areas and lower cross-areas. The critical temperature of steel as a criterion is used 538$^{\circ}C$ for column and 593$^{\circ}C$ for beam which is from ASTM E 119 because we don't make provisions as critical temperature by elements. We could consider that the best way of determination of fire protection thickness is using the following multi-regression equation which was deprived from several fire tests using the concept of section factor, FR(column) = 0.17 +5191.49t A/Hp + 40.77t, FR(beam) = 0.25 +6899.31t A/Hp + 32.60t(where, FR means fire resistant time, t means thickness, A means cross-area and Hp means heated parameter).

• Fire Science and Engineering
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• v.24 no.4
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• pp.86-91
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• 2010

In event of a tunnel fire, all kinds of equipment can be destroyed in high temperature that can exceed $1300^{\circ}C$, fatal structural demage can be caused by spalling of concrete structural elements. To make matters worse, there is a high possibility of the secondary damage which can lead to the collapse of the shear resisting structure. Accordingly, it is time that we developed the technology to counter fires in connection with the fire-resistant design of a tunnel structure. To secure the reliability of the fire-resistance performance of a tunnel structure, it is necessary to assess the fire's behavior on every structural element exposed to the fire as well as to calculate the tunnel fire intensity and the quantity of heat released. In this study, we drew out the fire damage range of each structural element of a tunnel and the minimum thickness of concrete cover for each fire-resistant material through some actual experiments of fire behavior on the structural elements of a tunnel.

• Fire Science and Engineering
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• v.34 no.4
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• pp.87-95
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• 2020

This study evaluates the safety of an asylum through a fire simulation of building Type traditional markets. We derive the building's indoor temperature, use the observed variation in temperature gradient to calculate the temperature of the main structure, and finally compares the time required to attain the limit temperature of the structure its time of escape. To ensure improved security of the asylum, the government has proposed a fire-resistance improvement plan for the major structural parts of buildings are not safe with thickness of 0.01 m and 0.035 m. F.ire-resistance reinforcement for small - and medium-sized vehicles is more than 0.025 m, in thickness; moreover safety can be ensured for medium and large-sized vehicles fire using fire resistant reinforcement of over 0.035 m. Accordingly, in order to ensure the safety of an asylum, fire-resistant reinforcement measures may be considered.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.21 no.3
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• pp.265-273
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• 2008

The problem controlling lateral drift by the wind and the earthquake is very important in high rise buildings. But, outrigger system, generally used for residential tall buildings in Korea, has weak points with the occupancy of special space, the difficult construction and the long duration of works. On the other hand, the damper reduces story drifts of building structure by absorbing vibration energy induced by the dynamic loads and the application of damper systems is relatively simple. Also, the lateral drift control system such as outrigger system may raise the wind vibration problem of serviceability like human comfort and this problem may need another vibration control devices. Accordingly, we analyze the effect of the drift control using various dampers to substitute for outrigger system as the efficient system in residential tall buildings.

• Journal of the Korea Safety Management & Science
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• v.12 no.3
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• pp.121-128
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• 2010

This is to show some basic data for introducing both circulated aggregate and recycled powder producing waste concrete. Standard-mixing design for 24MPa has been basically used and added and replaced normal aggregate with recycled powder made of waste concrete. In addition, polycarboxylate high-range water reducing agent has been used because recycled powder is missing adhesive strength and it is not compare with cement's adhesive strength. Compressive strength with powder mixture of 2%, 4%, 6%, 8%, and 10% has been decreased down to 80% of normal concrete material strength without recycled powder mixture. $200^{\circ}C$, $400^{\circ}C$ and $600^{\circ}C$ heated concrete were compressively tested in order to find out concrete strength resistant to high temperature. heat capacity was also tested, based on the expectancy of its low conductivity. In addition, thermal conduction test was tested in order to find out concrete insulation. According to this test, when concrete was tested by fire resistance, it using the circulation aggregate was same resulted by concrete using the natural aggregate. also, recycle powder was not effecting insulation performance. but it is fit to standard on concrete insulation of building law.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.2
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• pp.60-66
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• 2016

Polyurethane foam index a of the cell structure and the absorption change by using the foam stabilizer of six to investigate the polyurethane foam index producing the agent to the siloxane analyzed with silicon foam stabilizer with FE-SEM in accordance with the characteristics of the silicon-based foam stabilizer cell structure of the primary DC-193 on the chain ends is PO n dog bond, DC-2585, DC-5125, DC-198 has been confirmed as a close cell, silicone surfactant is combined EO n dog to a siloxane main chain terminus DC-5043 and DC-5598 that appeared to open cell structure. In addition, most absorption of the DC-5043 appeared was the size of the open cell greatest formed by the absorption of the cell structure change this absorption of the size of the close cell most detailed and uniform DC-193 appeared small household water-resistant best many showed. The performance test of the water was found to be excellent.

• Nuclear Engineering and Technology
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• v.50 no.3
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• pp.470-477
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• 2018

With a highly functional methyl vinyl silicone rubber (VMQ) matrix and filler materials of $B_4C$, PbO, and benzophenone (BP) and through powder surface modification, silicone rubber mixing, and vulcanized molding, a flexible radiation shielding and resistant composite was prepared in the study. The dispersion property of the powder in the matrix filler was improved by powder surface modification. BP was added into the matrix to enhance the radiation resistance performance of the composites. After irradiation, the tensile strength, elongation, and tear strength of the composites decreased, while the Shore hardness of the composites and the crosslinking density of the VMQ matrix increased. Moreover, the composites with BP showed better mechanical properties and smaller crosslinking density than those without BP after irradiation. The initial degradation temperatures of the composites containing BP before and after irradiation were $323.6^{\circ}C$ and $335.3^{\circ}C$, respectively. The transmission of neutrons for a 2-mm thick sample was only 0.12 for an Am-Be neutron source. The transmission of ${\gamma}$-rays with energies of 0.662, 1.173, and 1.332 MeV for 2-cm thick samples were 0.7, 0.782, and 0.795, respectively.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.205-212
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• 2009

Wheat (Triticum aestivum L.) grain texture is an important determinant of milling properties and end product use. Two linked genes, puroindoline a (PINA) and puroindoline b (PINB), control most of the genetic variation in wheat grain texture. Wheat seed proteins were examined to identify PINA and PINB gene using two pre-harvest sprouting wheat cultivars; Jinpum (resistant) and Keumgang (susceptible).Wheat seed proteins were separated by two-dimensional electrophoresis with IEF gels over pH ranges: pH 3-10. A total of 73 spots were digested with trypsin resulting peptide fragmentation were analyzed by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS). Mass spectra were automatically processed and searched through NCBInr, SWISS-PORT and MSDB database with mono isotopic masses and complete gene sequence were found by UniProt database. Puroindoline a and puroindoline b that is responsible for grain texture related with baking performance and roughness. Two spots were found Pin b (16.7 kDa) and Pin a (16.3 kDa) in Jinpum compare to seven spots were identified Pin a (16.1 kDa, 16.3 kDa) and Pin b (16.7 kDa, 9.5 kDa and 14.4 kDa) in Keumgang. Some selected spots were identified puroindoline like grain softness protein (16.9 kDa, 17 kDa and 18.1 kDa) in Keumgang. Moreover, to gain a better inferring the identification of puroindoline related proteins using proteomics, we accomplished a complete gene sequence of PINA and PINB gene in pre-harvesting sprouting wheat seeds between resistant (Jinpum) and susceptible (Keumgang).