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Proteomics Approach on Puroindoline Gene of Pre-harvest Sprouting Wheat  

Kamal, Abu Hena Mostafa (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Park, Cheol-Soo (Honam Agricultural Research Institute, National Institute of Crop Science)
Heo, Hwa-Young (Breeding Resource Development, National Institute of Crop Science)
Chung, Keun-Yook (Department of Agricultural Chemistry, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Cho, Yong-Gu (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Kim, Hong-Sig (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Song, Beom-Heon (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Lee, Chul-Won (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Woo, Sun-Hee (Department of Crop Science, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Publication Information
Korean Journal of Breeding Science / v.41, no.3, 2009 , pp. 205-212 More about this Journal
Abstract
Wheat (Triticum aestivum L.) grain texture is an important determinant of milling properties and end product use. Two linked genes, puroindoline a (PINA) and puroindoline b (PINB), control most of the genetic variation in wheat grain texture. Wheat seed proteins were examined to identify PINA and PINB gene using two pre-harvest sprouting wheat cultivars; Jinpum (resistant) and Keumgang (susceptible).Wheat seed proteins were separated by two-dimensional electrophoresis with IEF gels over pH ranges: pH 3-10. A total of 73 spots were digested with trypsin resulting peptide fragmentation were analyzed by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS). Mass spectra were automatically processed and searched through NCBInr, SWISS-PORT and MSDB database with mono isotopic masses and complete gene sequence were found by UniProt database. Puroindoline a and puroindoline b that is responsible for grain texture related with baking performance and roughness. Two spots were found Pin b (16.7 kDa) and Pin a (16.3 kDa) in Jinpum compare to seven spots were identified Pin a (16.1 kDa, 16.3 kDa) and Pin b (16.7 kDa, 9.5 kDa and 14.4 kDa) in Keumgang. Some selected spots were identified puroindoline like grain softness protein (16.9 kDa, 17 kDa and 18.1 kDa) in Keumgang. Moreover, to gain a better inferring the identification of puroindoline related proteins using proteomics, we accomplished a complete gene sequence of PINA and PINB gene in pre-harvesting sprouting wheat seeds between resistant (Jinpum) and susceptible (Keumgang).
Keywords
Proteomics; Puroindoline; Seed protein; Wheat (Triticum aestivum L.);
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