• Investigative Magnetic Resonance Imaging
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• 제25권4호
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• pp.300-312
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• 2021

Compressed sensing (CS) has been investigated in magnetic resonance (MR) parametric mapping to reduce scan time. However, the relatively long reconstruction time restricts its widespread applications in the clinic. Recently, deep learning-based methods have shown great potential in accelerating reconstruction time and improving imaging quality in fast MR imaging, although their adaptation to parametric mapping is still in an early stage. In this paper, we proposed a novel deep learning-based framework DEMO for fast and robust MR parametric mapping. Different from current deep learning-based methods, DEMO trains the network in an unsupervised way, which is more practical given that it is difficult to acquire large fully sampled training data of parametric-weighted images. Specifically, a CS-based loss function is used in DEMO to avoid the necessity of using fully sampled k-space data as the label, thus making it an unsupervised learning approach. DEMO reconstructs parametric weighted images and generates a parametric map simultaneously by unrolling an interaction approach in conventional fast MR parametric mapping, which enables multi-tasking learning. Experimental results showed promising performance of the proposed DEMO framework in quantitative MR T1ρ mapping.

• Journal of Nutrition and Health
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• 제45권4호
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• pp.315-323
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• 2012

Korean undergraduate students attempt to lose weight but often fail due to utilizing ineffective weight loss strategies. Some diet programs have succeeded, yet, they have not provided adequate skills for long-term weight maintenance. The aim of the study was to determine the effect of a low calorie diet and exercise with nutritional education on weight loss, serum lipid profiles, nutrient intakes, and dietary behavior modification in overweight and obese undergraduate students. The subjects in the low calorie diet group (LCD; n = 12) and the low calorie diet plus exercise group (LCDE; n = 13) had the same goal of losing 4 kg during a 12 week program. Nutrient intakes were assessed by the 24 hour recall method. Also, food habits and dietary behaviors were investigated by self-administered questionnaires before and after the weight control program and one month after completing the program. LCD and LCDE groups lowered body weight by 2 kg and 1 kg, respectively, although they decreased calorie intake by 355 and 287 kcal per day compared to intakes prior to the study. Body fat mass decreased in both the LCD and LCDE groups; however, the decrease was greater in the LCDE group. In addition, only the LCDE group increased muscle mass. The LCD group had a slightly better effect in reducing body weight, body fat, and waist circumference than the LCDE group. However, their decrease was reversed after the mid-study check in the LCD group; the reduction was better maintained and decreased more in the LCDE group. However, serum lipid profiles were already in borderline prior to the study; moreover, they were not modified after losing weight. The dietary behavior program helped students to develop better dietary habits. In conclusion, the combination of a low calorie diet and exercise is necessary in order to maintain longer weight loss by increasing muscle mass and decreasing body fat.

• 한국산업보건학회지
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• 제31권2호
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• pp.111-118
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• 2021

Objectives: The necessity of samples for analysis requested by working environment monitoring institutes (WMIs) has grown recently. The collection of samples of a few chemical substances requested by WMIs is allowed under the current occupational safety and health act in Korea, leading to an expansion of samples for analysis requested by institutes (SRIs). The objective of this study was to identify the number of institutes for analyzing requested samples (IARS), SRIs, and their participation in a non-mandatory proficiency test. Methods: Questionnaires were completed by all WMIs. The collected information was quantity of analysis equipment, sorting of chemicals from SRIs, and the number of SRIs. This was compared in terms of the participation in the non-mandatory proficiency test. Results: All WMIs in Korea responded to the survey, establishing a 100% response rate. There were 52 (29%) IARS among the 179 WMIs in Korea. The total number of samples of acid for ion chromatograph (IC) analysis requested by WMIs was 21,165, which is the most. Even the number of IARS for crystalline silicon oxide was less than other top-five IARS. The total amount of samples was 13,863, which was the second most. The calculated participation score for IARS was significantly higher than other WMIs (p<0.001). According to participation in the non-mandatory proficiency test by type of substance, such as crystalline silicon oxide and formaldehyde among IARS, the number of SRIs from those IARS was significantly higher than IARS that did not participate in the proficiency test (p<0.05). Conclusions: IARS had a high frequency of participation in the non-mandatory proficiency test and the number of SRIs at IARS participating in the proficiency test was higher among IARS. With the revision of the occupational health and safety act in Korea, the number of IARS participating in the non-mandatory proficiency test might increase.

• 기술혁신연구
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• 제20권2호
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• pp.1-28
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• 2012

그동안 출연연은 과학기술 발전을 선도하는 핵심 역할을 담당해 왔으나 최근에는 조직의 경쟁력 문제를 포함하여 그 미션과 역할까지 새롭게 정립해야 하는 시점에 있다. 본 연구는 ETRI를 결정적인 사례로 하여 현재 출연연이 직면하고 있는 구조적 한계와 어려움을 살펴보았다. 분석 결과 출연연의 자체 변화 노력도 문제이지만 출연연이 예산, 인력, 평가 등 통제가 어려운 구조적인 문제에 봉착되어 있음을 알 수 있다. ETRI의 경우 연구개발이나 특정기술 수준에서는 추격에서 탈추격으로 넘어서고 있으나 우리나라 출연(연) 연구 환경과 실제 일하는 방식은 추격형 체제에 머물러 있는 상황이다. 추격의 대상과 수단이 어느 정도 확실했던 추격기를 넘어 어디로 가야하며 무엇을 해야 할 것인가가 불확실한 탈추격 혁신 상황에서는 출연연의 미션 및 역할, 예산 인력 평가 체계, 사업기획 체계 및 프로세스 방식 등이 새롭게 변화될 필요가 있다.

• KSBB Journal
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• 제17권4호
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• pp.370-375
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• 2002

새로운 유전자를 클로닝하고 그 발현양상을 결정하는 것은 유전자의 기능을 이해하는데 필수적이다. 인간유전자 12q13의 고해상 물리지도를 작성하면서 이 지역의 D12S359와 D12S1618 사이에 내재하는 것으로 mapping된 stSG 3435 EST의 유전자를 클로닝하고 그 발현양상을 조사하였다. NIBI library를 조사하여 stSG 3435를 포함하는 클론 325E4를 분리하여 순차적 결실 방법으로 클로닝하여 자동염기서열분석으로 염기서열을 결정하였다. 1,331 bp의 염기서열을 가진 이 유전자는 Blast search에 의하면 376 개의 아미노산으로 이루어진 단백질로써 인간의 MYGI과 동일하며 마우스의Gamml, melanocyte proliferation gene 1과 86%의 동질성을 보였다. MYGI은 인간염색체의 12에 내재하며 마우스의 Gamml은 syntenic 부위인 마우스 염색체 15에 내재하므로 마우스의 Gamml의 homolog으로 간주된다. Northern blot analysis 결과 MYG1은 인간의 모든 조직에서 발현되며 정소에서 가장 강한 발현을 보였다. 이 유전자의 세포내 발현을 green fluorescence protein과 융합시켜 발현 귀착지를 confocal 현미경으로 동정한 결과 MYG1 단백질은 핵과 리소좀을 제외한 소기관에서 발현되는 것을 관찰하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1999년도 하계학술대회 논문집 B
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• pp.606-608
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• 1999

Mutual Recognition Agreement(MRA) between national metrology institutes is started on October 1999 and International Key Comparison is essentially to be used as technical basis for the agreement. High resistance is one of the Key Comparison and high resistance measurement system is established in KRISS for the purpose of the Key Comparison. Total combined uncertainty of the system is about 4ppm in $10M{\Omega}$ and 8ppm in $1G{\Omega}$. With the system, the difference of comparison results for $10M{\Omega}$ and $1G{\Omega}$ among KRISS, VNIIM in Russia and NIM in China is agreed about 6ppm within total combined uncertainty of three institutes.

• 기술혁신학회지
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• 제8권2호
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• pp.832-859
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• 2005

Government-sponsored Research Institutes (GRIs) have made a great contribution to the development of Korea's Science and Technology (S&T). However, recently, GRIs are facing the challenge of many problems, for instance, absence of reasonable strategies, insufficient resources, and so on. Therefore, many GRIs are required for solving these problems by enhancing their strategic management capabilities. For this purpose, this paper suggests a Strategic Management Audit Model for Korean GRIs. The concept of Strategic Management Audit Model has not been widely adopted in Korean GRIs. In this paper, we suggest GRI-specific Strategic Management Model and apply it to Korea Basic Science Institute (KBSI), which is under the Korea Research Council of Fundamental Science and Technology (KRCF). Based on our strategic management audit, we propose several potential strategies for Korean Basic Research Institutes (KBSI). Our model and its implications will also be helpful for other Korean GRIs.

• Industrial Engineering and Management Systems
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• 제6권1호
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• pp.49-54
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• 2007

This study deals with technology planning-based management for Basic R&D Programs of government supported research institutes (GRIs). The Korean Research Council for Industrial Science and Technology which consists of major GRIs needs to implement new government policies which focus on the dissemination of R&D outcome, collaborative research, and performance-oriented R&D investment. The major characteristics of technology planning-based management include the hierarchy of market-oriented technology plans and the valuation of core technologies. We also suggest technology classifications and project network diagrams.

• 대한미생물학회지
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• 제21권1호
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• pp.151-161
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• 1986

In order to develop sensitive and sepcific assay methods for E. coli heat labile enterotoxin(LT) hybridoma cell lines secreting LT specific monoclonal antibody were obtained. LT was purified from cell lysate of E. coli O15H11. The steps included disruption of bacteria by French pressure, DEAE Sephacel ion exchange chromatography, Sephadex G200 gel filtration, and second DEAE Sephacel ion exchange chromatography, successively. Spleen cells from Balb/c mice immunized with the purified LT and $HGPRT^{(-)}$ plasmacytomas, $P3{\times}63Ag8.V653$ were mixed and fused by 50% (w/v) PEG. Hybrid cells were grown in 308 wells out of 360 wells, and 13 wells out of them secreted antibodies reacting to LT. Among these hybridoma cell 1G8-1D1 cell line was selected since it had produced high-titered monoclonal antibody continuously. By using culture supernatant and ascites from 1G8-1D1 cells the monoclonal antibody was characterized, and an assay system for detecting enterotoxigenic E. coli was established by double sandwich enzyme-linked immunosorbent assay (ELISA). The following results were obtained. 1. Antibody titers of culture supernatant and ascites from 1G8-1D1 hybridoma cells were 512, and 102, 400, respectively by GM1-ELISA and its immunoglobulin class was IgM. 2. The maximum absorption ratio of 1G8-1D1 cell culture supernatant to LT was 90% at $300\;{\mu}g/ml$ of LT concentration. LT concentration shown at 50% absorption ratio was $103.45{\mu}g$ and the absorption ratio was decreased with tile reduction of LT concentration. This result suggests that monoclonal antibody from 1G8-1D1 hybridoma cell bound with LT specifically. 3. The reactivities of 1G8-1D1 cell culture supernatant to LT and V. cholerae enterotoxin(CT) were 0.886 and 0.142(O.D. at 492nm) measured by the GM1-ELISA, indicating 1G8-1D1 monoclonal antibody reacted specifically with LT but not with CT. 4. The addition of 0.1ml of ascites to 0.6mg and 0.12mg of LT decreased the vascular permeability factor to 41% and 44% respectively, but it did not completely neutralize LT. 5. By double sandwich ELISA using monoclonal antibody, as little as 75ng of the purified LT per ml could be detected. 6. The results by assay of detecting LT in culture supernatants of 14 wild strains E. coli isolated from diarrhea patients by the double sandwich ELISA were almost the same level as those by reverse passive latex agglutination.

• 디지털융복합연구
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• 제15권5호
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• pp.161-172
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• 2017

본 연구의 목적은 공공연구기관 연구자의 연구자율성 구성요소를 새로운 관점에서 정의함과 동시에 그러한 구성요소들이 조직효과성에 미치는 영향을 살펴봄으로써, 궁극적으로 조직효과성 제고를 위해 공공연구기관 연구자에게 보장되어야 할 연구자율성 요소는 무엇인지를 확인하고 정책적인 지원 방안을 제시하는 데 있다. 이를 위한 기초 작업으로 연구자율성 수준을 구성 형태에 따라 개인 및 조직 수준으로, 연구자율성 유형을 본질적 및 비본질적 자율성으로 구분하였으며, 조직효과성에 미치는 영향을 알아보기 위해 조직몰입도와 직무만족도를 측정하였다. 연구 결과 본질적 자율성이 비본질적 자율성에 비해 조직효과성에 미치는 영향이 매우 높게 나타났다. 이는 연구자의 연구수행을 위한 간접적인 지원환경도 중요하지만 연구과제 선정, 자유로운 의견 교환, 연구방법론 선택, 연구결과 활용 등 연구 본연의 활동에 있어서 연구자들의 의사결정 및 재량권이 월등히 중요하다고 할 수 있을 것이다.