The Journal of the Korean Society for Microbiology (대한미생물학회지)
- Volume 21 Issue 1
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- Pages.151-161
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- 1986
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- 0253-3162(pISSN)
Development of Assay Methods for Enterotoxin of Escherichia coli Employing the Hybridoma Technology
잡종세포종기법을 이용한 대장균의 장독소 측정법 개발
- Kim, Moon-Kyo (Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
- Cho, Myung-Je (Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
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Park, Kyung-Hee
(Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
- Lee, Woo-Kon (Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
- Kim, Yoon-Won (Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
- Choi, Myung-Sik (Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
- Park, Joong-Soo (Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
- Cha, Chang-Yong (Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
- Chang, Woo-Hyun (Department of Microbiology, Cancer Research Institutes, College of Medicine, Seoul National University) ;
- Chung, Hong-Keun (Department of Biochemistry, Cancer Research Institutes, College of Medicine, Seoul National University)
- 김문교 (서울대학교 의과대학 미생물학교실 및 암연구소) ;
- 조명제 (서울대학교 의과대학 미생물학교실 및 암연구소) ;
-
박경희
(서울대학교 의과대학 미생물학교실 및 암연구소) ;
- 이우곤 (서울대학교 의과대학 미생물학교실 및 암연구소) ;
- 김윤원 (서울대학교 의과대학 미생물학교실 및 암연구소) ;
- 최명식 (서울대학교 의과대학 미생물학교실 및 암연구소) ;
- 박중수 (서울대학교 의과대학 미생물학교실 및 암연구소) ;
- 차창용 (서울대학교 의과대학 미생물학교실 및 암연구소) ;
- 장우현 (서울대학교 의과대학 미생물학교실 및 암연구소) ;
- 정홍근 (서울대학교 의과대학 생화학교실 및 암연구소)
- Published : 1986.03.31
Abstract
In order to develop sensitive and sepcific assay methods for E. coli heat labile enterotoxin(LT) hybridoma cell lines secreting LT specific monoclonal antibody were obtained. LT was purified from cell lysate of E. coli O15H11. The steps included disruption of bacteria by French pressure, DEAE Sephacel ion exchange chromatography, Sephadex G200 gel filtration, and second DEAE Sephacel ion exchange chromatography, successively. Spleen cells from Balb/c mice immunized with the purified LT and
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