• Journal of Plant Biology
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• v.30 no.1
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• pp.43-57
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• 1987

The epidermal structure and stomatal types in vegetative and reproductive organs of three species of Bryophyllum(B. crenatum, B. diagremontion, B. tubiflorum) were described. The epidermal cells were polygonal, isodiametric, and rectangular in the leaves and stems, and elongated cells in the stamens, styles, and ovaries. These cells were commonly thick, and arched or sinuous in the leaves, epiphylous bunds, petals and ovaries. They were straight in the stems, petioles, pedicels, and peduncles. In both vegetative and reproductive organs, the subsidiary cell walls were commonly thin and mostly arched in all the organs. The great majority of the mature stomata in all the organs were helicocytic type with a helix of four to six subsidiary cells. The mature stomata varied from organ to organ with regard to the number and arangement of subsidiary cells. The ontogenetic type of stomata in all the organs was mostly helico-eumesogenous type. This type was subdivided into three subtypes such as parahelico-eumesogenous, anomohelico-eumesogenous, and diahelico-eumesogenous stomata on the basis of the division angle fo the guard mother cell. Sometimes, the anisoeumesogenous type was found in various organs. This type was subdivided into three subtypes such as paraniso-eumesogenous, anomoaniso-eumesogenous, and dianisoeummesogenous stomata. The tetra-eumesogenous and duplotetra-eumesogenous types were rarely found; the former in the leaf of B. crenatum and the latter in the leaf of B. diagremontiana. Anomometric patterns in the mesogenous categorry of stomatal types was observed in a few organs of all the materials. A new stomatal type with tetra-eumesogenous stoma within a girdle of three subsidiary cells fo aniso-eumesogenous in the leaf of B. diagremontiana was firstly observed in the vascular plants. This stoma was termed the cotetra-aniso-eumesogenous type. Anormal stomata such as aborted stomata, single guard cells, stoma with a constricted part in the middle of large guard cells, and arrested stomata were found in the various organs of all the materials.

• The Korea Journal of Herbology
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• v.27 no.2
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• pp.37-42
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• 2012

Objectives : The purpose of this study is to examine the antioxidant effects on male mouse reproductive cells of the extract of Platycladi Semen. Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay, lipid peroxidation by malondialdehyde (MDA) formation and super oxide dismutase (SOD), respectively. Results : The results showed that the extract scavenged DPPH radical in a dose-dependent manner by up to 74.87%. The cell viability of the extract was within 72~96% on Leydig cells and GC-2 cells at concentrations of 1, 5, 10, 50 and 100 ug/ml. The hydrogen peroxide-induced cytotoxicity of Leydig cells was protected to 72.09% by the extract at concentration of 100 ${\mu}g/ml$. The hydrogen peroxide-induced lipid peroxidation of MDA formation was decreased to 1.80 and 1.65 nmoles/mg protein by the extract at concentrations of 50 and 100 ${\mu}g/ml$. The extract at all concentrations, SOD activity was not significantly changed. Conclusions : In conclusion, the extract of Platycladi Semen has antioxidant effects on Leydig cells and protect male reproductive system against oxidative stress.

• Clinical and Experimental Reproductive Medicine
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• v.46 no.2
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• pp.76-86
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• 2019

Objective: This study was performed to explore the possibility that each oocyte and its surrounding cumulus cells might have different genetic expression patterns that could affect human reproduction. Methods: Differential gene expression analysis was performed for 10 clusters of cumulus cells obtained from 10 cumulus-oocyte complexes from 10 patients. Same procedures related to oocyte maturation, microinjection, and microarray analyses were performed for each group of cumulus cells. Two differential gene expression analyses were performed: one for the outcome of clinical pregnancy and one for the outcome of live birth. Results: Significant genes resulting from these analyses were selected and the top 20 affected pathways in each group were analyzed. Circadian entrainment is determined to be the most affected pathway for clinical pregnancy, and proteoglycans in cancer pathway is the most affected pathway for live birth. Circadian entrainment is also amongst the 12 pathways that are found to be in top 20 affected pathways for both outcomes, and has both lowest p-value and highest number of times found count. Conclusion: Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy.

• 대한생식의학회:학술대회논문집
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• 2007.11a
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• pp.15-22
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• 2007

• 대한생식의학회:학술대회논문집
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• 2007.06a
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• pp.15-16
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• 2007

• 대한생식의학회:학술대회논문집
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• 2001.03a
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• pp.237-247
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• 2001

• 대한생식의학회:학술대회논문집
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• 2006.06a
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• pp.58-59
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• 2006

• Journal of Embryo Transfer
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• v.24 no.3
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• pp.139-143
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• 2009

Most captive canids and felids at Zoos in advanced countries have been examined enough to apply artificial reproductive techniques to them. We investigated reproductive hormones and vaginal epithelial cells of a 6-year-old, female coyote, hoping these data could eventually be extended to artificial insemination with frozen-thawed conspecific semen at Seoul Zoo. As a relative of pet dogs, coyote exhibited a similar appearance with only minor differences. In vaginal smear, an increase in the number of superficial cells suggests that the bitch has reached a state close to estrus. A sudden decrease of estradiol and increase of progesterone is considered as a preovulatory event. Vaginal epithelial cells and hormones might be useful for determining the optimal time of artificial insemination in coyotes' breeding.

• Journal of Plant Biology
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• v.37 no.4
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• pp.421-428
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• 1994

The vegetative and male reproductive anatomy of a marine alga, Laurencia intercalaris sp. nov. (Rhodomelaceae, Rhodophyta), is described from subtidal habitats of eastern and southern Korea. This species has terete thalli with entangled fibrous holdfasts and regularly alternate branching of ultimate branchlets, and is inseparable from L. okamurae Yamada on the basis of habit. Vegetative axial cells produce a trichoblast and four pericentral cells in an alternating sequence. Spermatangia are produced intercalary or subterminally from one of two laterals on suprabasal cells of trichoblasts arising from axial cells in apical pits of branchlets. The other lateral remains sterile. In this sterile lateral, budding-like regeneration occurs on older segments that are oabscised. Comparison is made with other related Laurencia species, particularly those with terete thalli. The vegetative anatomy and the regeneration in sterile laterals of male trichoblasts, with the mode of spermatangial formation, distinguish the new species from previously described species of Laurencia including L. okamurae.

• Journal of Embryo Transfer
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• v.18 no.2
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• pp.109-113
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• 2003

The study was carried out to investigate the effects of morphology, preservation and reproductive cycle of oocytes in vitro maturation of cats oocytes and development of IVM embryos. The results were summarized as follows : 1. Nuclear status of GV and MI of in vitro cultured(24 h) oocytes with and whithout cumulus cells were 74.3% and 25.7%, 28.6% and 11.4%, 77.1% and 5.7%, respectively, The rate of oocytes with cumulus cells was higher than that of denuded oocytes. 2. Nuclear status of GV and MI of in vitro cultured(24 h) oocytes recovered from ovaries collected at different stages of the reproductive cycle(inactive, follicular and luteal) were 88.6% and 6.5%, 60.0% and 11.4%, 77.1% and 5.7%, respectively. 3. Nuclear status of fresh and salts-stored oocytes with and whithout cumulus cells were 74.3%, 25.7% and 37.1%, 11.4% and 57.1%, 13.3%, 17.1%, 3.3%, respectively. The rate of oocytes with cumulus cells(13.3%∼74.3%) was higher than that of denuded oocytes(3.3%∼57.1%).