Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Differential gene expression analysis of human cumulus cells

  • Demiray, Sirin Bakti (Assisted Reproduction Unit, Tepecik Education and Research Hospital) ;
  • Goker, Ege Nazan Tavmergen (Department of Obstetrics and Gynecology, Ege University Faculty of Medicine) ;
  • Tavmergen, Erol (Department of Obstetrics and Gynecology, Ege University Faculty of Medicine) ;
  • Yilmaz, Ozlem (Department of Histology and Embryology, Ege University Faculty of Medicine) ;
  • Calimlioglu, Nilufer (Department of Obstetrics and Gynecology, Ege University Faculty of Medicine) ;
  • Soykam, Huseyin Okan (Epigenetiks Genetics Bioinformatics Software Inc.) ;
  • Oktem, Gulperi (Department of Histology and Embryology, Ege University Faculty of Medicine) ;
  • Sezerman, Ugur (Department of Biostatistics and Bioinformatics, Acibadem Mehmet Ali Aydinlar University, Institute of Health Sciences)
  • Received : 2018.11.16
  • Accepted : 2019.02.02
  • Published : 2019.06.30
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Abstract

Objective: This study was performed to explore the possibility that each oocyte and its surrounding cumulus cells might have different genetic expression patterns that could affect human reproduction. Methods: Differential gene expression analysis was performed for 10 clusters of cumulus cells obtained from 10 cumulus-oocyte complexes from 10 patients. Same procedures related to oocyte maturation, microinjection, and microarray analyses were performed for each group of cumulus cells. Two differential gene expression analyses were performed: one for the outcome of clinical pregnancy and one for the outcome of live birth. Results: Significant genes resulting from these analyses were selected and the top 20 affected pathways in each group were analyzed. Circadian entrainment is determined to be the most affected pathway for clinical pregnancy, and proteoglycans in cancer pathway is the most affected pathway for live birth. Circadian entrainment is also amongst the 12 pathways that are found to be in top 20 affected pathways for both outcomes, and has both lowest p-value and highest number of times found count. Conclusion: Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy.

Keywords

References

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